Anwar Rosyidi
Faculty Of Animal Husbandry, University Of Mataram, Jl Majapahit No 62 Mataram, Nusa Tenggara Barat, Indonesia 83125

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Campylobacter jejuni seroepidemiology in native chicken Rosyidi, Anwar; Budiharta, S; Asmara, W; Yudhabuntara, D
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 3 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.581 KB) | DOI: 10.14334/jitv.v17i3.706

Abstract

Campylobacter jejuni is responsible for about 90% of cases of Campylobacteriosis in humans with gastroenteritis. Healthy chickens can carry Campylobacter spp. in the intestinal tract. Efforts to reduce exposure to Campylobacteriosis by humans may be enhanced by knowledge of its prevalence in poultry. This study aimed to identify factors associated with seropositive response to C. jejuni in native chickens in Mataram. Detection of C. jejuni was accomplished using an immunochromatographic serological method. Association between Campylobacter jejuni seropositive response as the dependent variable with various independent variables was analyzed using χ² (Chi square) and Odds Ratio (OR). A total of 216 chicken samples were examined and 44 chicken owners were interviewed and their farms examined. Results showed the prevalence of serological response to C. jejuni in chicken samples to be as high as 35.6% and that as many as 70.5% of farms had affected chickens.  Age of the chicken was the variable most closely associated with incidence of seropositive response, birds older than 3 months more likely to be affected. Variables at the farm level associated with variation in seropositive response were cage type, cage floor material, and origin of drinking water, surface water sources being less desirable. Key Words: Campylobacter jejuni, Seroepidemiology, Native Chicken, Mataram
Skrening Bakteri Asam Laktat yang Diisolasi dari Usus Ayam Broiler sebagai Kandidat Probiotik untuk Unggas Nurbaiti Nurbaiti; Anwar Rosyidi; Muhamad Ali
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 2 No 1 (2016): Jurnal Ilmu dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Scie
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (77.093 KB) | DOI: 10.29303/jitpi.v2i1.25

Abstract

The use of probiotics is increasingly popular as an alternative replacement to antibiotic uses in stimulating healthand growth of broiler chickens. The use of antibiotics may disturb the balance of the gut microbiota and play asignificant role in the emerging of antibiotic resistance pathogens. Thus, the objective of this study was to isolateand screen lactic acid bacteria (LAB) from the intestine of broiler chickens as probiotic candidates. LAB wereinitially screened by phenotypic assay. Furthermore, those strains which belonged to LAB were screened forinhibitory activity against E. coli. Results showed that a total of 4 isolates were considered as members of lacticacid bacteria indicating by the results of phenotypic assays: gram positive, catalase and oxidase negative, and ableto perform carbohydrate fermentation. Out of the 4 isolates, only one isolate showed an inhibitory activity againstthe targeted pathogen. Further research is still needed to confirm the potential strain for probiotic in poultry.
Endoparasitic Infestation Case on Buffalo (Bubalus bubalis) in West Praya District of Central Lombok Muhammad Hipzul Mursyid; Anwar Rosyidi; Wayan Wariata; Made Sriasih
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 6 No 1 (2020): Jurnal Ilmu dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Scie
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.222 KB) | DOI: 10.29303/jitpi.v5i2.66

Abstract

The purpose of this study was to determine endoparasitic infestation on Buffaloes in Praya Barat district, Central Lombok. Stools samples were taken from 61 buffaloes from 5 villages and examined at Banyumulek Animal Health Laboratory of Animal Husbandry and Animal Health Service of NTB Province using the floating and sedimentation methods to perceive the presence of parasitic eggs. The data obtained were analyzed descriptively. The results show that from 61 fecal samples analyzed, 20 samples (32.7%) were positively infected with endoparasites with a single type of infection and multi-species infection. A total of 13 samples (21.3%) were infected with Nematodes, 2 samples (3.27%) were infected with Cestode, 2 samples (3.27%) were infected with Trematode and 11 samples (18.03%) were infected with Protozoa. The degree of endoparasitic infection in buffaloes in West Praya district is included in the category of mild infection. Endoparasites that infect buffaloes in Praya Barat are coming from family Toxocaridae, Trichostrongylidae, Cooperidae, Anoplocephalidae, Strongylidae, Strongyloididae, Chabertidae, Fasciolidae and Eimeriidae. The highest prevalence for worm parasite was Toxocaridae with the percentage of 11.46%, whilst for the protozoa was the family of Eimeriidae accounted for 18.01%.
Skrening Resistensi Antibiotik Pada Bakteri Asam Laktat yang Diisolasi dari Usus Ayam Pedaging Muhamad Ali; Nurbaiti Nurbaiti; Anwar Rosyidi; Muhamad Ichsan
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 4 No 1 (2018): Jurnal Ilmu dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Scie
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.02 KB) | DOI: 10.29303/jitpi.v3i1.39

Abstract

Antibiotic use in poultry production is commonly performed as the feed additive for promoting the health and growth of the chicken. However, excessive use of antibiotics may lead to the emergence of antibiotic resistance in Escherichia coli, lactic acid bacteria, and pathogenic bacteria. In this research, screening of antibiotic resistance was assayed on lactic acid bacteria obtained from broiler intestine. Among the 14 lactic acid isolates, 2 (14%) isolates were found to be resistant to chloramphenicol. Resistance was also observed to ampicillin and kanamycin (7%). The result of this research indicated that the overuse and misuse of antibiotics in broiler farm tend to generate antibiotic resistance in broiler’s intestine lactic acid bacteria. Therefore, some advice for safety measure of antibiotic application in poultry farmer is strongly necessary.
Infection and Distribution of Liver Fluke (Fasciola Sp) Zoonotic Parasite on Cattle in Central Lombok and East Lombok Districts Wayan Wariata; Made Sriasih; Anwar Rosyidi; Muhamad Ali; Sulaiman N. Depamede
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 5 No 2 (2019): Jurnal Ilmu dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Scie
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.317 KB) | DOI: 10.29303/jitpi.v5i1.55

Abstract

Salah satu faktor yang sangat mempengaruhi populasi dan produksi ternak adalah masalah penyakit dan parasit. Gejala serangan parasit seringkali tidak tampak dan kadang-kadang petani kurang menyadarinya. Cacing hati (Fasciola sp.) merupakan parasit zoonosis yang dapat menginfeksi berbagai macam hewan ternak ruminansia terutama sapi. Fasciolamemiliki dua spesies utama yaitu hepatica dan gigantica. Penyebaran Fasciola sp. adalah daerah beriklim tropis basah yaitu di Asia dan Afrika. Fasciola sp. yang sering dijumpai di Indonesia adalah spesies gigantica, sedangkan spesies hepatica sering terdeteksi pada sapi-sapi impor. Untuk mengetahui infeksidan tingkat penyebaran Fasciola giganticadan Fasciola hepaticapada ternak sapi di kabupaten Lombok Tengahdan Lombok Timurmaka dilakukan survei, pengamatan dan pengambilan sampel organ hati sapi di pasar-pasar daging yang ada pada dua daerah tersebut. Pada masing-masing daerah diambil 3 lokasi pasar daging, yaitu di kabupaten Lombok Tengah: pasar Praya, pasar Mantangdan pasar Kopang; di kabupaten Lombok Timur: pasarSelong, pasarMasbagikdan pasarTerara. Pada masing-masing pasar, pengamatan dan pengambilan sampel diulang sebanyak 3 kalipada 3 orang pedagang.Selanjutnya sampel diamati di laboratorium untuk mengetahui adanya cacing hati dan kerusakan hati sapi. Hasil penelitian menunjukkan bahwa, sapi-sapi dan daging sapi yang diperjual belikan di pasar-pasar Lombok Tengah dan Lombok Timur terinfeksi parasit cacing hati (Fasciola sp.), dengan tingkat infeksi yang masih rendah, yaitu antara 1,3 sampai 2,3. Di samping itu, warna dan tekstur hati sapi yang diperjual belikan di pasar-pasar Lombok Tengah dan Lombok Timur relatif masih baik dan layak dikonsumsi.
Campylobacter jejuni seroepidemiology in native chicken Anwar Rosyidi; S Budiharta; W Asmara; D Yudhabuntara
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 3 (2012): SEPTEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.581 KB) | DOI: 10.14334/jitv.v17i3.706

Abstract

Campylobacter jejuni is responsible for about 90% of cases of Campylobacteriosis in humans with gastroenteritis. Healthy chickens can carry Campylobacter spp. in the intestinal tract. Efforts to reduce exposure to Campylobacteriosis by humans may be enhanced by knowledge of its prevalence in poultry. This study aimed to identify factors associated with seropositive response to C. jejuni in native chickens in Mataram. Detection of C. jejuni was accomplished using an immunochromatographic serological method. Association between Campylobacter jejuni seropositive response as the dependent variable with various independent variables was analyzed using χ² (Chi square) and Odds Ratio (OR). A total of 216 chicken samples were examined and 44 chicken owners were interviewed and their farms examined. Results showed the prevalence of serological response to C. jejuni in chicken samples to be as high as 35.6% and that as many as 70.5% of farms had affected chickens.  Age of the chicken was the variable most closely associated with incidence of seropositive response, birds older than 3 months more likely to be affected. Variables at the farm level associated with variation in seropositive response were cage type, cage floor material, and origin of drinking water, surface water sources being less desirable. Key Words: Campylobacter jejuni, Seroepidemiology, Native Chicken, Mataram
Pengaruh Penyemprotan Air Panas, Asam Asetat Dan Asam La Ktat Terhadap Perkembangan Jumlah Mikroba Pada Karkas Kambing Anwar Rosyidi; Doodi Yudhabuntara; Setyawan Budiharta
Jurnal Sain Veteriner Vol 23, No 1 (2005): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1166.824 KB) | DOI: 10.22146/jsv.357

Abstract

Penelitian ini bertujuan mengetahui sejauh mana efektivitas air panas, asam asetat dan asam laktat dalam menghambat perkembangan mikroba pada karkas kambing. Paha kambing diperoleh dari rumah pemotongan hewan Ngampilan Yogyakarta. Masing-masing kelompok diberi perlakuan sebagai berikut : kelompok I tanpa penyemprotan (kontrol), kelompok II disemprot dengan asam asetat 1,8 %, kelompok III disemprot dengan dengan asam laktat 1,5 % (v/v), kelompok IV disemprot dengan air panas 80 °C diikuti asam laktat 1,5 % (v/v) dan kelompok V disemprot dengan asam asetat 1,8 % (vlv) diikuti dengan asam laktat 1,5 % (vlv). Hasil penelitian menunjukkan bahwa perkembangan jumlah total koliform pada kelompok kontrol lebih tinggi dibandingkan pada keempat kelompok perlakuan.. Perkembangan jumlah total koliform lebih tinggi pada kelompok asam laktat 1,5 %, kelompok asam asetat 1,8 % dibandingkan dengan kelompok kombinasi asam asetat 1,8 % dan asam laktat 1,5 % (P
Pengembangan Metode Imunokromatografi untuk Deteksi secara Serologi Campylobacter jejuni pada Ayam DEVELOPMENT OF IMMUNOCHROMATOGRAPHIC METHOD FOR DETECTING Campylobacter jejuni ON CHICKEN SEROLOGICALLY) Anwar Rosyidi; Setyawan Budiharta; Widya Asmara; Doddi Yudhabuntara
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.599 KB)

Abstract

Campylobacter spp cause infection in human in the form of gastroenteritis and Guillain-Barre syndrome.Campylobacter jejuni is one of  Campylobacter spp responsible for about 90% of cases of Campylobacteriosisin human with gastroenteritis.  Efforts to prevent the incidence of Campylobacteriosis in humans should bestarted with an assessment on its level of poultry. This study aims to develop a diagnostic test for C. jejunion chicken using immunochromatographic serological method. Stages of the research consist of thepreparation of test card, antigen preparation, optimization of the antigen and serum. The results showedthat immmunochromatographic card can distinguish infected serum from the uninfected.The minimumamount of antigen  was found to be 1,5 ng/µl. It needs 10 µl serum to perform the test properly.
Potensi Air Liur Sebagai Perantara dalam Pemeriksaan Noninvasive pada Hewan Piaraan (POTENTIAL OF SALIVA AS A MEDIATOR FOR THE NONINVASIVE EXAMINATION OF DOMESTIC ANIMALS) Sulaiman Ngongu Depamede; Anwar Rosyidi; Made Sriasih; Dahlanuddin .; Enny Yulianti; Suparman .
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.584 KB)

Abstract

Saliva as unique biological components of the oral cavity has potential as a mediator for noninvasivebiological test. For future livestock industry the application of noninvasive test is strongly necessary dueto animal welfare porpuse. Until now the research and development of the using saliva as a mediator forinvasive test is predominant for humans, while for livestock or domestic animals is still limited. Techniquesand methods that are commonly used are related to proteomics analysis. With this method a lot of thingsthat can be brought about proteomics of saliva are beneficial to the development of saliva-based biomarkers.Although this method is expensive, researches on the use of saliva in the field of livestock industries areurgently needed. It is expected that noninvasive biological test methods based on saliva as a mediator canbe performed immediately.
Rerative Risk of Management Factors which Influence Infertility of Artificial Insemination in Bali Cattle in East Lombok Anwar Rosyidi; Adji Santoso Dradjat; Lalu Ahmad Zainuri; Lalu Wirapribadi; Tjok. Suwendra Binetra; Haryanto Haryanto
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.299 KB) | DOI: 10.19087/jveteriner.2021.22.2.292

Abstract

The aim of this study was to evaluate odds ratio (OR) of management factors on infertility of artificial insemination results. Three traditional farmer groups were purposively choosed, based on percentage of calf born of AI program. Group I, with calves born of 61.22%, Group II with calves born of 38,89 %, and group III with calves born of 30,0%. The management factors evaluated were 1. ownerships of cattle, 2. raise cattle in separate pen with other, 3. cattle raised in pen dirty pen, 4. not enough feed given 5. body condition score. The data was obtained by dichotomy questioner and observation then evaluated using odds ratio (OR), when OR more than 1 indicated the high risk of factor to infertility. The results of OR in Group I showed that ownership (1,18), separated pen (0,54), dirty pen (2,11), less feed (3,20) and body condition (0,22). The results in Group II showed that ownership (0,83) , separated pen (1,12), dirty pen (0,14), less feed (2,08) and body condition (2,00). The results of odds ratios in Group III showed that ownership (1,63), separated pen (3,83), dirty pen (0,88), less feed (1,00) and body condition (0,12). These data indicated that ownerships, dirty pen, less feed and body condition were factors which may confounding with other factors. However less OR of separate pen were, in Group I (0,54), Group II (1,12) and Group III (3,83) consistently with number of calves produced. It can be concluded that separate pen was a factor which influence calves produced in using artificial insemination.