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Isolasi dan Aktivitas Antibakteri Aktinomisetes Asal Tanah Gambut Riau Linda, Tetty Marta; Roza, Rodesia Mustika; Yuliati, Rola; Wahyuliyanti, Wahyuliyanti
Jurnal Natur Indonesia Vol 10, No 1 (2007)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.074 KB) | DOI: 10.31258/jnat.10.1.18-23

Abstract

The aims of this study are to isolate actinomycetes from peat soil samples, to determine the ability of actinomycetes to inhibit the growth of Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus) and Gram negativebacteria (Escherichia coli and Pseudomonas sp.). A total of 14 actinomycetes strains were recovered from peat soil samples using pour plate method with Starch Casein Agar. The results showed that 11 isolates were active against B. subtilis, 8 isolates against S. aureus, 8 isolates were active against E. coli and 8 isolates againstPseudomonas sp. Two isolates (SM 1.3 and SM 1.6) were active against all bacterial targets.
Seleksi Potensi Bakteri Probiotik Indigenus Riau sebagai Agen Biopreservatif Bahan Pangan WULANDARI, NUR ASYIAH; FIBRIARTI, BERNADETA LENI; ROZA, RODESIA MUSTIKA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

The use of synthetic preservatives is not safe for consumption and very dangerous for health.Lactic acid bacteria (LAB) is a probiotic bacterium that can produce bacteriocins asbiopreservatives agents. Bacteriocins have been widely applied as a food preservative, but itscommercial availability is still low and very expensive. This study was conducted to test the abilityof 23 isolates of local probiotic bacteria in Riau to produce bacteriocins. LAB total population wascalculated by Total Plate Count (TPC) method. LAB inhibition test against indicator bacteriaStaphylococcus aureus FNCC 0047 was conducted using well diffusion method. Bacteriocinsantimicrobial confirmatory test was performed at 100OC for 15 minutes, 30 minutes, and 60minutes. The result showed that the highest LAB population was PSG4 (22.45 x 106 cfu/ml) andthe lowest was SLK2 (4.75 x 106 cfu/ml). The greatest inhibitory activity against indicator bacteriaS. aureus FNCC 0047 was found in PSG2 (15.1 mm) and the smallest was in SLK2 (10.75 mm)with the activity of bacteriocins 2575 mm2/ml and 792.6 mm2/ml respectively. The bacteriocinsconfirmatory test showed clear zone of isolates JN5, PSG2, and SLK2 with the largest inhibitionzone was during heating for 30 minutes and 60 minutes (19.2 mm, 19.5 mm, and 16.8 mm) whilethe smallest was for 60 minutes and 15 minutes (12.8 mm, 15.6mm, and 14.7 mm).Keywords: Probiotic bacteria, biopreservatives, bacteriocins, Staphylococcus aureus FNCC 0047.
UJI POTENSI ANTIFUNGI AKTINOMISETES SELULOLITIK DAN LIGNINOLITIK DAN BAKTERI LIGNOSELULOLITIK ISOLAT LOKAL TERHADAP PERTUMBUHAN JAMUR Ganoderma boninense DAN Colletotrichum capsici Dede Martin; Atria Martina; Rodesia Mustika Roza
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Colletotrichum capsici fungi, causing agent of anthracnose in chili, and Ganoderma boninense, fungi causing agent of basal stem rotten in oil palm, can reduce the productivity of chili and oil palm. These disease are increasing so that it is necessary to find local biological agents that are environmentally friendly. The purpose of this research was to determine the potential isolates of lignocellulolyltic bacteria and cellulolytic and ligninolytic actinomycetes from peat soil of Rimbo Panjang Kampar, Riau as an antifungal agent to inhibit the growth of C. capsici and G. boninense.Antifungal activity was screened using agar disc method by measured the inhibition zone for seven days. The results showed that 13 isolates of actinomycetes haveantifungal activity against C. capsici with the highest inhibition zone 13,3 mm by RB2S40. Six isolates of actinomycetes have antifungal activity against G. boninense with the highest inhibition zone 29,15 mm by RB1S4. Five isolates have the ability to inhibit both fungi which were targeted.
Inovasi teknologi hidroponik melalui pemanfaatan barang bekas dan nutrisi buatan sendiri sebagai usaha peningkatan pendapatan petani di Desa Kualu Kecamatan Tambang Kabupaten Kampar Fitmawati Fitmawati; Mayta Novaliza Isda; Isnaini Isnaini; Nery Sofiyanti; Rodesia Mustika Roza
Unri Conference Series: Community Engagement Vol 1 (2019): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.1.499-505

Abstract

The hydroponic technology of the wick system is one of the innovations in vegetable cultivation without soil with the innovation of utilizing used goods and household waste such as styrofoam and used cans as a container for planting. The purpose of this activity is to reduce waste in the community, improve the family's economic capacity, and people's interest in consuming clean and hygienic vegetables in Kualu Village, Tambang District, Kampar Regency. This hydroponic technique is made of relatively inexpensive and affordable materials so that the limitations of people's financial capabilities can be overcome. This system is a hydroponic system that uses axes to absorb organic nutrients. To minimize the cost of purchasing nutrients, it can be overcome by innovating nutrient solutions from household waste into liquid fertilizer. The implementation of community service uses counselling, discussion and making hydroponic technology. The community is very enthusiastic in joining this program, especially housewives who want to know the application of Wick's hydroponic technology so that they can play a large role in meeting food needs and increasing people's income.
Antioxidant Activity of Dominant Plants Species in Obat Pahit from Lingga Malay Ethnic in Riau Archipelago Fitmawati, Fitmawati; Sofiyanti, Nery; Roza, Rodesia Mustika; Isnaini, Isnaini; Irawan, Yulisa Resti; Winata, Dhaniel Ridho; Dewi, Awal Prichatin Kusumo
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9808

Abstract

Obat Pahit is a potion that has been long commonly consumed by Lingga Malay society for generations as stamina keeper. The most dominant plants found in the packaging of the Obat Pahit were namely Bauhunia semibifida, Cnestis palala and Penawa Root (3 species). This research aimed to investigate and determine activity of antioxidant contents in Obat Pahit from five Traditional Medicine Practitioners (TMPs) in the district of Lingga. The tested samples were mashed then being soaked into 2 types of solvent: distilled water and methanol, containing HCl 1%. DPPH method was also used in this research. Quantitatively antioxidant activity test of Obat Pahit from the five TMPs by using methanol solvent had extremely highest activity compared to the distilled water solvent. The test, using TLC plate by spraying the extract from three dominant plants with 0.1 mM of DPPH solution, produced a pale-yellow spots at a wavelength of 366 nm. On the other hand, the test using HPLC at wavelengths of 230 nm and 280 nm showed the presence of two dominant secondary metabolites contents: flavonoid and phenolic. IC50 (ppm) of Bauhinia semibifida (6.6247), Penawa Root (5.0124) and Cnestis palala (5.9968) were much lower than IC50 of mangosteens rind (41.7675), vitamin C (6.6612) and Stimuno drug (8.333). This antioxidant analysis has not been reported previously. This proof contributed greatly to uncovering potentially native natural resources as an indigenous Indonesian drug which is expected to decrease dependence on imported drugs especially imunomodulator, antihypertensive, antidiabet etc. This research would be beneficial and excellent manifestation for the development of natural antioxidant-based medicines from traditional knowledge of Indonesias local ethnicities.
Immunomodulatory Effectiveness of Aqueous Obat Pahit Extract of Lingga Malay Ethnic on White Rats (Rattus novergicus) Fitmawati, Fitmawati; Roza, Rodesia Mustika; Sofiyanti, Nery; Isnaini, Isnaini; Fitri, Febrian Lailatul; Paramita, Desi; Dewi, Awal Prichatin Kusumo
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10496

Abstract

Obat pahit has been generally known and believed by Lingga Malay society as anti-aging agent. However, the study of Obat pahit is not scientifically proven. This research was aimed to prove immunomodulatory ability of Obat pahit potion from Lingga, Riau Archipelago. This study used white rats as an animal modelling, and Staphylococcus aureus as bacteria tester. The rats had been treated with aqueous Obat pahit extract from three TMPs on dose scales of 0.09, 0.18 and 0.27 mL/200g of body weight through oral administration for 7 days. Furthermore, on the 8th days, the experiment animals were injected by the preparation of bacteria tester through intraperitoneal administration in the amount of 0.5 mL/200 gram of body weigth and subsequently incubated for 1 hour after the injection. There were 2 observed parameters on this study, i.e efectivity and capacity of phagocytosis by leukocytes. The observation of leukocytes-phagocytocis activity was carried out by making a smear preparat samples of peritoneum fluid from rats. After the observation under microscope on a magnification of 100 times. The result was obtained the Obat pahit from Kalan PMT swere more effective on dose 2, while from SP4 and Linau TMPs were much more effective on dose 1. It is therefore, using these data of the results, the advanced doses scale of this Obat pahit would not be necessary. Obat pahit potion from Malay Lingga Malay Ethnic could become raw materials of immunomodulatory herbal medicine based on traditional knowledge. It also potentially as a standardized herbal.
Seleksi Isolat Aktinomisetes Asal Tanah Gambut Desa Rimbo Panjang Kabupaten Kampar Dalam Menghasilkan Hormon IAA (Indole Acetic Acid) MAWARTI, INDAH; FIBRIARTI, BERNADETA LENI; ZUL, DELITA; ROZA, RODESIA MUSTIKA; MARTINA, ATRIA; LINDA, TETTY MARTA
Jurnal Riau Biologia Vol 2, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Hormon IAA (Indole Acetic Acid) termasuk fitohormon golongan auksin yang berperan sebagai zat pemacu pertumbuhan tanaman. Selain tumbuhan, mikroba juga diketahui mampu menghasilkan IAA. Penelitian ini bertujuan untuk menyeleksi kemampuan koleksi isolat aktinomisetes Laboratorium Mikrobiologi FMIPA UR dalam menghasilkan IAA. Uji produksi IAA oleh aktinomisetes dilakukan dengan penambahan reagen Salkowski menggunakan metode kolorimetri dalam medium SCB (Starch Casein Broth) yang diperkaya dengan triptofan sebagai prekursor dan tanpa triptofan. Hasil penelitan ini diperoleh sebanyak 50 dari 85 isolat aktinomisetes yang memiliki kemampuan dalam menghasilkan hormon IAA. Produksi IAA tertinggi dihasilkan oleh RB5S78 sebesar 35,97 ppm dan konsentrasi terendah dihasilkan oleh RB4S67 sebesar 0,24 ppm dalam medium SCB yang diperkaya triptofan. Pada medium tanpa triptofan hasil tertinggi diperoleh isolat aktinomisetes RB5S78 dengan konsentrasi sebesar 15,28 ppm dan konsentrasi terendah dihasilkan oleh RB4S65 sebesar 0,16 ppm. Isolat aktinomisetes yang menghasilkan IAA dengan kriteria tinggi yang diperoleh termasuk dalam genus Streptomyces dan Rhodococcus.
The Potential of Wild Mango Leaves from Sumatera as the Immunostimulant Agent Fitmawati, Fitmawati; Juliantari, Erwina; Saputra, Agus; Sinaga, Patar; Roza, Rodesia Mustika; Isda, Mayta Novaliza
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (740.186 KB) | DOI: 10.15294/biosaintifika.v10i3.16549

Abstract

Wild Mango from Sumatera is potentially new immunostimulant. It contains mangiferin, which is potentially become the immunostimulant candidate. This study aimed to prove the immunostimulatory effect of wild mango leaves extract on white rat peritoneum fluid induced by Staphylococcus aureus bacteria. The immunostimulatory effect was determined based on the activity and capacity of macrophage phagocytosis. This study used 54 male white rats about 130 g - 290 g. Based on the results,the highest phagocytosis activity of macrophage cells was found in ethanol extract of Mangga Hutan leaves dose 2 with a percentage of phagocytosis activity was 84%. The highest phagocytosis capacity of the macrophage cells was also found in ethanol extract of Mangga Hutan leaves dose 2 with an average phagocytic capacity of 171.67 from 50 active macrophages. Results of this study indicated that wild mango leaves from Sumatera has the potential immunostimulant activity. This indicates that the wild mango have the potential for therapeutic efficacy for the prevention of degenerative diseases caused by immune deficiencies.
AKTIVITAS ANTIBAKTERI EKSTRAK DAUN Cyclea barbata (L.)Miers. TERHADAP BAKTERI Escherichia coli DAN Salmonella typhi Arifan Asmardi; Rodesia Mustika Roza; Fitmawati '
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Indonesia has many medicinal plant species that are distributed in many regions. Green grass jelly (Cyclea barbata (L.) Miers.)is one of medicinal plant that used in traditional medicine by Indonesian people. This species used to treat inflammation, fever and others. The main content of green grass jelly are saponins, flavonoids and alkaloids. Escherichia coli and Salmonella typhi can infect the human body, but the green grass jelly can inhibit the growth of these bacteria. This study was performed using four methods, maceration, fresh leaf grinding, fresh and dried leave boiling, the dilution series (10%, 25%, 50%, 75% and 100%) were applied for each method. The best result among four methods was the maceration method with 100% concentration dilution. The treatment using E. coli showed the widest diameter of inhibition zone was 17.23 mm and the lowest diameter  was 7,70 mm. while the treatment using S. typhi showed the widest diameter was 15.89 mm and the lowest diameter was 6,85 mm. The higher  the concentrations used, the wider the inhibition zone diameter was.
ISOLASI DAN SELEKSI BAKTERI ASAM LAKTAT DARI YOGHURT PRODUKSI INDUSTRI RUMAH TANGGA DI PEKANBARU YANG BERSIFAT ANTIBAKTERI TERHADAP Escherichia coli DAN Salmonella typhi Lili Yani; Rodesia Mustika Roza; Atria Martina
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

The occurance of Lactic Acid Bacteria (LAB) in yoghurt produced by home industry inPekanbaru as probiotic potentially improve intestinal physiological function, useful forhealth as well as prevention of the growth of pathogenic bacteria. The purposes of thisstudy were to calculate the total LAB population of yoghurt with the effect of mango andstrawberry juice during different storage process, and also to determine the ability ofantibacterial activity against Escherichia coli and Salmonella typhi. The population of LABin yoghurt was 4,0 × 10 7 – 2,9 × 10 8 CFU/ml. Antimicrobial activity assay used the welldiffusion method. The mango juice yoghurt incubated in 7 day was the most effective forEscherichia coli with the inhibition zone 27,1 mm. While the mango juice yoghurtincubated in 3 day was the most effective for Salmonella typhi with inhibition zone 29,9mm.