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All Journal Jurnal Ilmu dan Teknologi Kayu Tropis

Rita K Sari

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Author-ID : 3652413

Agriculture, Biological Sciences & Forestry

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Aktivitas Antioksidan dan Tabir Surya Ekstrak Daun Gyrinops versteegii (Antioxidant activity and Sunscreen of Gyrinops versteegii Leaf Extract) Maeda Wahyuningrum; Rita K Sari; Mohamad Rafi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 16, No 2 (2018): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The aim of this study was to determine the yield and phytochemicals, antioxidant activity, and sunscreen of the Gyrinops versteegii leaf extracts. The leaf simplicia was extracted using soxhletation method with multilevel polarities of solvent (n-hexane, ethyl acetate, and metanol). The analysis of phytochemical extracts has been carried out the qualitatively and quantitatively. The antioxidant activity testing was performed in vitro through the effective concentration (EC50) extract in capturing DPPH radicals. Sunscreen activity has been done through testing sun protection factor (SPF). The result showed that the yield of n-hexane extract, ethyl acetate, and methanol extracts were 7.83, 5.46, and 6.77% respectively. The phytochemical analysis showed that the ethyl acetate and methanol extracts were strongly detected containing antioxidant compounds such as p-hydroquinone, flavonoid, and tannins with the total phenol of the ethyl acetate and methanol extracts were 3.40 and 4.27% respectively. The n-hexane extract detected contains weakly the antioxidant compounds with the total phenol was 0.45%. The methanol extract is the highest antioxidant activity (EC50 14.46 μg ml-1) and has ultra sunscreen activity (SPF>15).

Chemical Composition and Anticancer Activity Of the Essential Oil Of Surian Heartwood Rita K Sari; Wasrin Syafii; Suminar S Achmadi; Muhammad Hanafi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 9, No 2 (2011): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Chemical constituents of the essential oil of water and steam distillates of surian heartwood (Toona sinensis) were analyzed by a gas chromatograph-mass spectrometer. Then, the oil was investigated in vitro anticancer bioassay for its possible antioxidant activity by DPPH free radical scavenging and antiproliferative effects by MTT method using Raji lymphoma cancer cell lines (ATCC CCL 86), HeLa cervical cancer cell lines (ATCC CCL2), and Vero normal cell lines (ATCC CCL 81). The essential oil yield from water and steam distillates of surian heartwood was 0.4% (w/w). Thirty-two compounds were identified, that consisted mainly of oxygenated sesquiterpenes (77%). The most representative compounds were spathulenol (21%), isospathulenol (7%), α-cadinol (7%), β-cedrenoxide (6%), and torreyol (5%). The oil showed less effective antioxidant, but the oil had high antiproliferative effects on Raji and HeLa cancer cells with IC50 28 and 134 μg ml-1, respectively when compared to Vero normal cells (IC50 1412 μg ml-1). Our findings suggest that the essential oil of surian heartwood might be considered as a potentially anticancer agent on human cancer cells, especially lymphoma cancer cells.Key words: antioxidant activity, antiproliferative effects, cancer cell lines, essential oil, Toona sinensis

Perubahan Kadar Komponen Kimia Bambu Andong akibat Perlakuan Steam (Change of Chemical Components Content in Andong Bamboo due to Steam Treatment) Muhammad I Maulana; Deded S Nawawi; Nyoman J Wistara; Rita K Sari; Siti Nikmatin; Sena Maulana; Se-Hwi Park; Fauzi Febrianto
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 16, No 1 (2018): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Steam and rinsing treatment with water and 1% NaOH solution of andong bamboo strands have been known to improve physical and mechanical properties of its oriented strand board (OSB). The improvement of bamboo OSB properties might be caused by the change of chemical components content during steam and rinsing process. This study aims to measure the changes of chemical components content in andong bamboo (Gigantochloa pseudoarundinacea) due to steam treatment and rinsing with water or sodium hydroxide solution. Samples was taken from andong bamboo stem without bark and node part. The treatments of samples were steam at 126 ° C for 1 hour, steam and rinsing with water, and, steam and rinsing with 1% NaOH, respectively. Air-dried samples were, then, subjected to mill in order to get 40-60 mesh particles size, which was used for chemical components analyses. The results showed that steam and rinsing with water or 1% NaOH solution treatment reduced hemicellulose and extractive contents (dissolved in cold water, hot water, ethanol-benzene and NaOH 1% solution), whereas alpha-cellulose and lignin were relatively stable. Steam and rinsing treatment generally increased the pH value of bamboo samples.

Eksplorasi Tumbuhan Obat Hutan Berkhasiat Inhibitor HMG-KoA Reduktase (Exploration of Medicinal Forest Plants with HMG-CoA Reductase Inhibitory Activity) Sulistiyani Sulistiyani; Rita K Sari; Wulan Triwahyuni
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 15, No 2 (2017): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Five medicinal forest plants are potential candidates to be developed as cholesterol-reducing herbal product. The objective of this research is to explore the potency of the leaf of pulai ((Alstonia scholaris, R.Br), jabon merah (Antocephalus macrophyllus), jati belanda (Guazuma ulmifolia), mindi (Melia azedarach), and surian (Toona sinensis) based on their main secondary metabolic contents and to study their activity as HMG-CoA reductase inhibitor, an enzyme key in the regulation of cholesterol biosynthesis. Dried leaves materials were prepared from the each plant by standard procedure and their pythochemicals contents were determined qualitatively. Subsequently, flavonoid fractions from the leaves of all five plants were prepared, and alkaloid fraction was prepared from the leaf of pulai only. The flavonoid content was determined using quercetin as standard. The effect of each samples on the HMG-CoA reductase activity were analyzed using HMGR in vitro assay kit. At concentration of 20 ppm jati belanda leaf extract, however, showed the highest inhibitory activity which was similar to quercetin (5 ppm) as well as pravastatin control inhibitor. Further analysis showed that extracts from leaves of all plants tested were shown to inhibit the HMG-CoA reductase with IC50 values ranging from 7.53 to 52.62 ppm.Keywords: HMG-KoA reductase inhibitor, in vitro assay, medicinal forest plant exploration

Characterization of Cat’s Eye Dammar for Revision of Indonesia National Standard Rita K Sari; Nyoman J Wistara; Arif Wijayanto; Totok K Waluyo
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 11, No 1 (2013): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The present works was aimed to examine the concordance between trader quality classification and related SNI classification on dammar resin. The quality of six quality classes of dammar resin by trader classification was evaluated based on SNI 2900-1-2012 on visual classification and SNI 01-2900-1999 on physical and chemical characteristics, and then the resulting data were analyzed with ANOVA. The quality concordance of dammar resin based on trader classification with these of SNI for visual appearance and the size of agglomeration was 50 and 75-94%, respectively, and these of insolubility in toluene, melting point, ash content, and acid number were 61, 72, 11, and 100%, respectively. The characteristics of dammar resin for the a, b, and c quality classification were the same for the Krui traders, BKG Ltd., and WGM Ltd. However, they were different for the lower quality classification of d, e, and powder. It was also found the existence of dammar resin with characteristic values beyond the range of these established by SNI. SNI should not classify dammar resin quality based on visual appearance, but merely based on its physical and chemical characteristics. Physical and chemical characteristics values required by SNI should be revised.Key words: cat’s eye dammar, Indonesia National Standard, physical and chemical characteristics, quality classification, Shorea javanica

Anticancer Activity and Chemical Compounds of Suren Heartwood Extract (Toona sureni) Rita K Sari; Wasrin Syafii; Suminar A Achmadi; Muhammad Hanafi; Yanotama T Laksana
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 10, No 1 (2012): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The aims of this research were to determine the yield extracts from continuous extraction of suren heatwood (Toona sureni) in n-hexane, ethyl acetate and methanol solvents, to evaluate this extracts using in vitro anticancer tests (antioxidant, brine shrimp lethality test /BSLT, antiproliferative effects to HeLa servical cancer cell lines, Raji limphoma cancer cell lines, and Vero normal cell lines), and to analyze the best extract based on the anticancer activities. This experiment showed that the yield of methanolic, ethyl acetate, and n-hexane extracts were 0.43%, 0.25%, and 0.18% respectively. The methanolic and ethyl acetate extracts had the high antioxidant activities with EC50 51 and 68 μg ml-1 respectively. Based on BSLT, the ethyl acetate extract was the most active extract (LC50 40 μg ml-1), it followed by the methanolic extract (LC50 116 68 μg ml-1 ), and the n-hexane extract (LC50 161 68 μg ml-1). Further testing showed that the ethyl acetate extract had high antiproliferative effects to Raji (IC50 31 μg ml-1) and HeLa (IC50 65 μg ml-1), but it was more secure against Vero cell lines (IC50 105 μg ml-1). Whereas compounds such as catechol, linalool, and sitosterol contributed to the high anticancer activities of this ethyl acetate extract.Key words: anticancer activity, HeLa cell lines, Raji cell lines, Toona sureni, Vero cell lines

Co-Authors Arif Wijayanto Deded Sarip Nawawi Fauzi Febrianto I Nyoman Jaya Wistara Maeda Wahyuningrum Maulana, Sena Mohamad Rafi Muhammad Hanafi Muhammad Iqbal Maulana Se-Hwi Park Siti Nikmatin SULISTIYANI SULISTIYANI Suminar A Achmadi Suminar S Achmadi Totok K Waluyo Wasrin Syafii dan Kurnia Sofyan Gustan Pari Buchari Wulan Triwahyuni Yanotama T Laksana

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