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The Effects of Xanthorrhizol on the Morphology of Candida Cells Examined by Scanning Electron Microscopy YAYA RUKAYADI; JAE-KWAN HWANG
Microbiology Indonesia Vol. 1 No. 2 (2007): August 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1504.15 KB) | DOI: 10.5454/mi.1.2.11

Abstract

The effects of xanthorrhizol, a natural anticandidal agent isolated from the rhizome of temulawak or java turmeric (Curcuma xanthorrhiza Roxb.) on the morphology of four human pathogenic Candida species, i.e., C. albicans, C. glabrata, C. guilliermondii, and C. parapsilosis was examined by scanning electron microscopy (SEM). The SEM analysis showed that, unlike control cells representing normal oval to spherical with smooth surface, treatment of Candida strains with xanthorrhizol at 1 x MICs (minimum inhibitory concentration) significantly affected the external morphology, exhibiting deformation, and protrusions on the cell surface. The potent anticandidal activity of xanthorrhizol may support the use of medicinal plants for the treatment of candidal infections.
Time-Kill Assay of 4-Hydroxypanduratin A Isolated from Kaempferia Pandurata Against Foodborne Pathogens Soerya Dewi Marliyana; Didin Mujahidin; Yana M Syah; Yaya Rukayadi
Molekul Vol 12, No 2 (2017)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.658 KB) | DOI: 10.20884/1.jm.2017.12.2.363

Abstract

Time–kill assay was performed for 4-hydroxypanduratin A that was isolated from Kaempferia pandurata rizhome against four important foodborne pathogens, namely Bacillus cereus ATCC 21772, Bacillu subtilis ATCC 6633, Staphylococcus aureus ATCC 29737,  and  Proteus mirabilis ATCC 21100. The methods have been investigated in term of Minimum Inhibitory Concentration (MIC) and killing time curve using methods of Clinical and Laboratory Standards Institute (CLSI) guidelines. The results showed that 4-hydroxypanduratin A rapid acting in killing bacteria as follow: B. cereus : 1×MIC for 4 h, P. mirabilis: 4×MIC for 0.5 h, meanwhile  B. subtilis and S. aureus were 1×MIC for 2 h. In conclusion, 4-hydroxypanduratin A showed strong antimicrobial activity against four important foodborne pathogens.