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KEANEKARAGAMAN ASPERGILLUS PADA BERBAGAI TRADISIONAL SIMPLISIA JAMU Rukmi, Isworo
JURNAL SAINS DAN MATEMATIKA Volume 17 Issue 2 Year 2009
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

ABSTRACT---The  presence  of Aspergillas sp. in the simplisia  commonly consumed by Javanese people as a  traditional  medicine has been done. The  eight  simplisia  examined were:  kunyit  rhizome (Cucurma domesfica Val.), temuireng  rhizome (Cucurma aeroginosae), daun sambiloto (Andrographis paniculata), temulawak rhizome (Cucurma xanthorhizu), and mahkota  dewa (Phaleria macrocarpa). Sixteen Aspergillus  species were found from the samples, came from the niger,  flavus, versicolor, wentii, fumigatus, ochraceus dan ornatus  group.  Isolates found  in  this  study  were A. parositicus, A. flavas,  A. oryzae, A. tamarin, A. clavato-flavus, A. flavofurcatis, A. tubingiensis, A. awamori, A. ochraceus, A. sulphureus, A. melleus, A. ornatus, A. brunneo-uniseriatus, A. versicolor, A.  wentii, and A. fumigatus,  the first six are the member of flavus group  that  found  in  all  simplisia samples. Nine species of the isolatesknown as mycotoxin producers.Keywords : Aspergillus, simplisia, mikotoksin
Flavonoids Production Capability Test of Tea Mistletoe (Scurrula atropurpurea BL . Dans) Endophytic Bacteria Isolates Priyanto, Jepri Agung; Pujiyanto, Sri; Rukmi, Isworo
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 4 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Tea Mistletoe (S. Atropurpurea BL. Dans) is a medicinal plant species used as anticancer because it contains some flavonoids compounds are chalcones, c-glycoflavonols, chatechin and quercetin. The ability of endophytic bacteria to produce similar bioactive compounds with its host plant is potential source to get flavonoids compounds. This research aims to know ability of endophytic bacteria in produce flavonoids compounds in vitro. Each isolate was fermented in submerged culture with 0.1% soluble starch, 0.5% pepton, and 0.15% yeast extract medium for 5 days, then extracted with ethyl acetate. Flavonoid content of the extract then was tested qualitatively and confirmation test used thin layer chromatography. Qualitative test results showed that crude extract from isolates B4, B5, B10, B17, and B19 positive containing flavonoids. The most potent extract were B10 and B19 tested by thin layer chromatography. Two of these extracts had the same Rf value with quercetin, thus endophytic bacteria from tea mistletoe can produce flavonoids in vitro.
Indigenous Trichoderma harzianum as Biocontrol toward Blight Late Disease and Biomodulator in Potato Plant Productivity Purwantisari, Susiana; Sitepu, Harum; Rukmi, Isworo; Lunggani, Arina Tri; Budihardjo, Kadarwati
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.26706

Abstract

Indigenous Trichoderma has widely used in late-blight epidemic areas in Magelang, Indonesia as biocontrol and biomodulator. But it application still lacks of scientific proof, even though it is potently to be developed. Therefore, this study aims to identify and evaluate indigenous Trichoderma application in eradicating late blight and increase productivity. This research was an experimental posttest-only using six experimental plots, with P0 plot with no treatment. The solution was made by dissolve indigenous Trichoderma solid-starter into water (m/v), then used for treatment. The treatment plots including: two groups of sprayed-only plots with doses of 100 g/ 10 L of solution for P1 and 150 g/ 10 L of solution for P2; and two group treated with sprayed-poured method using doses of 100 g/ 10 L of solution for P3 and 150 g/ 10 L of solution for P4. The positive control group was P5 group, treated using chemical pesticides and tuber booster. The microscopic identification revealed that the local bioagent was Trichoderma harzianum species. Application of T. harzianum in P4 was significantly increased the productivity. However, it was not effective in increasing growth, but able to reduce the intensity of late blight disease. The research revealed that native Trichoderma is able to be used as anti-infectious agent and potentially improve the quality of potato plants. In the future, this research may worthwhile for farmers to develop and produce trustworthy and proven Trichoderma-based biocontrol and help them increase the potato production economically.
Aktivitas Enzimatis Biakan Kapang Aspergillus Section Nigri DUCC (Diponegoro University Culture Collection) Dan Identifikasi Molekuler Isolat Potensial Sabrini, Zalia; Rukmi, Isworo; Ferniah, Rejeki Siti
Bioma : Berkala Ilmiah Biologi Vol. 23, No 1, Tahun 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.23.1.1-5

Abstract

The enzyme amylase, cellulase and protease are an extracellular enzymes are many produced by microorganisms such as fungi Aspergillus section Nigri. This research aims to find out the potential biakanes of Aspergillus section Nigri DUCC collection (Diponegoro University Culture Collection) which is capable to produce some enzymes that consists of amylase, protease and celullase and also identification in molecular based. Activity enzymatic assay of Aspergillus section Nigri with  semi-quantitative method using selective medium, CMC for cellulolytic, Starch agar 1% for amylolytic and Skim Milk agar for proteolytic. Biakane potential is determined by looking at the Enzymatic Index (EI) is highest for all of three enzyme. Molecular identification is using the universal primer ITS4 and ITS5. The results showed that the culture of DUCC K207  has high activity for all of three enzyme. Index enzymatic of isolate DUCC K207 , 1.55 mm for amylolytic, 1.49 mm for cellulolytic and 1, 24 mm for proteolytic. Result of molecular identification DUCC K207  known as Aspergillus niger that has 100% similarity with Aspergillus niger MH 109325.1.
Indigenous Trichoderma harzianum as Biocontrol toward Blight Late Disease and Biomodulator in Potato Plant Productivity Purwantisari, Susiana; Sitepu, Harum; Rukmi, Isworo; Lunggani, Arina Tri; Budihardjo, Kadarwati
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.26706

Abstract

Indigenous Trichoderma has widely used in late-blight epidemic areas in Magelang, Indonesia as biocontrol and biomodulator. But it application still lacks of scientific proof, even though it is potently to be developed. Therefore, this study aims to identify and evaluate indigenous Trichoderma application in eradicating late blight and increase productivity. This research was an experimental posttest-only using six experimental plots, with P0 plot with no treatment. The solution was made by dissolve indigenous Trichoderma solid-starter into water (m/v), then used for treatment. The treatment plots including: two groups of sprayed-only plots with doses of 100 g/ 10 L of solution for P1 and 150 g/ 10 L of solution for P2; and two group treated with sprayed-poured method using doses of 100 g/ 10 L of solution for P3 and 150 g/ 10 L of solution for P4. The positive control group was P5 group, treated using chemical pesticides and tuber booster. The microscopic identification revealed that the local bioagent was Trichoderma harzianum species. Application of T. harzianum in P4 was significantly increased the productivity. However, it was not effective in increasing growth, but able to reduce the intensity of late blight disease. The research revealed that native Trichoderma is able to be used as anti-infectious agent and potentially improve the quality of potato plants. In the future, this research may worthwhile for farmers to develop and produce trustworthy and proven Trichoderma-based biocontrol and help them increase the potato production economically.
Pengaruh Vitamin B Kompleks Pada Produksi Senyawa Antimicrobial Peptides dari Pediococcus pentosaceus Serta Uji Aktivitasnya Terhadap Bacillus cereus dan Eschericia coli Febrianty, Debby Ananda; Wijanarka, W; Rukmi, Isworo
Bioma : Berkala Ilmiah Biologi Vol. 23, No 2, Tahun 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.23.2.133-142

Abstract

Both eukaryotic and prokaryotic organisms can produce antimicrobial peptides (AMPs) which function as a self-defense mechanism against other harmful organisms in the same ecological niche. Pediococcus pentosaceus is a species of Lactic Acid Bacteria (LAB) capable of producing AMPs in the form of bacteriocins and bacterocin-like inhibitory substances. The compounds it produces have received Generally Regarded as Safe status by the Food and Drug Association (FDA) and have potential as biological preservatives in the food sector. The production of these compounds can be influenced by environmental factors and growth medium of producing bacteria, so that optimization studies for the production of bacteriocin have been developed, in order to obtain optimal its activity. Vitamin B Complex is one of the growth factors needed by living things including bacteria to support their metabolism. This study aims to determine whether the addition of vitamin B complex affects the production and activity of AMPs from P. pentosaceus. Vitamin B Complex concentrations of 0 ppm, 0.1 ppm, 1 ppm and 10 ppm were added to the production medium of P. pentosaceus. Cell-free supernatants were harvested by centrifugation, then their activity was tested against Bacillus cereus and Eschericia coli using the Kirby Bauer method and analyzed using one way ANOVA parametric statistical test with a significance level of 0.05 and Duncan's post hoc test. The results showed that the addition of vitamin B complex was not significantly different to the activity of AMPs compounds, but in higher concentrations could reduce the activity of these compounds.