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Apoptotic Potential of Secretome from Interleukin-Induced Natural Killer Cells toward Breast Cancer Cell Line by Transwell Assay Wahyu Widowati; Diana Krisanti Jasaputra; Teresa Liliana Wargasetia; The Fransiska Eltania; Alya Mardhotillah Azizah; Mawar Subangkit; I Nyoman Ehrich Lister; Chrismis Novalinda Ginting; Ermi Girsang; Ahmad Faried
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.186

Breast cancer (BC) is the number one cause of deaths from cancer in women. Metastasis in BC is caused by immunosurveillance deficiency, including impairment of Natural Killer (NK) cell maturation, low NK activity, and decreasing cytotoxicity. This study was performed to improve activating receptors and cytotoxicity of NK cells using interleukin 15 (IL15) against BC cells. Human recombinant IL15 was used to induce NK cells. To evaluate the potential of IL15 in inducing NK cells, we measured the activating and inhibiting receptors (NKG2D, NKG2A), apoptotic potency of NK cells on BC cells (MCF7) using transwell assay. The IL15 inducer on the NK cell were measured NKG2D, NKG2A gene expression with quantitative polymerase chain reaction (qPCR), (GzmB) secretion using ELISA, apoptotic gene expression of MCF7 using qPCR. IL15 increased NKG2D expression 4.01-9.13%, but IL15 could not affect toward NKG2A expression on NK cells. IL15-activated NK cells, inhibited BC cells proliferation, induced apoptotic BC cells 25.89-32.19%, induced apoptotic genes of BC cells bax, p53. IL15 increase NK activating receptor (NKG2D), inhibit BC cells proliferation, induce apoptotic percentage and induce apoptotic gene expression.

AKUMULASI LIPID HATI DAN PROFIL LIPID DARAH TIKUS Sprague Dawley YANG DIINTERVENSI MINYAK SUPER OLEIN DAN OLEIN Naufal Muharam Nurdin; Rimbawan Rimbawan; Sri Anna Marliyati; Drajat Martianto; Mawar Subangkit
Jurnal Gizi dan Pangan Vol. 11 No. 1 (2016)
Publisher : Food and Nutrition Society of Indonesia

The objective of this research was to analyze the effect of olein and super olein palm oil on blood lipid profile (total cholesterol, LDL cholesterol, HDL cholesterol, and triglyceride) and fatty liver condition of Sprague Dawley rats. The design used was pre and post test with control group design. The intervention run for 8 weeks using 20 rats which were divided into four treatments i.e: (1) standard-low fat feed and water (negative control/KN), (2) standard-low fat feed and dried egg yolk (positive control/KP), (3)standard-low fat feed and olein palm oil (B), and (4) standard-low fat feed and super olein palm oil (A). Results showed that blood lipid profile of rats at baseline were not different among treatment groups, while at midline super olein group has total cholesterol, triglyceride, and indirect LDL cholesterol lower than olein group. At endline, HDL and indirect LDL cholesterol of super olein group were different compared to olein group, while total cholesterol and triglyceride were not different between both groups. Super olein group had HDL cholesterol higher and indirect LDL cholesterol lower than olein group. Based on histopathology, lipid accumulation in the liver of Sprague Dawley rats in super olein group were lower than in olein group. The data showed that percentage of lipid droplet in super olein group (A) was 22.01%, while in olein group (B) was 40.43%.Keywords: blood lipid profile, fatty liver, palm oil, rats

Spatial learning and memory of young and aging rats following injection with human Wharton’s jelly‐mesenchymal stem cells Berry Juliandi; Wildan Mubarok; Dian Anggraini; Arief Boediono; Mawar Subangkit; Indra Bachtiar; Harry Murti; Kelvin Yaprianto; Boenjamin Setiawan
Indonesian Journal of Biotechnology Vol 26, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.64734

Human Wharton’s jelly‐mesenchymal stem cells (hWJ‐MSC) are an emerging potential source of stem cells derived from the umbilical cord. Previous studies have shown their potential as treatment for traumatic brain injury and Parkinson’s disease. However, no study has yet investigated the effect of hWJ‐MSC injections in countering spatial learning and memory impairment in aging rats. The effect of hWJ‐MSC injection on young rats is also unknown. The objective of this research was to analyze the effect of an hWJ‐MSC injection on spatial learning, memory, density of putative neural progenitor cells (pNPC), and neuronal apoptosis in the dentate gyrus (DG) of young and aging rats. Injection of hWJ‐MSC did not change spatial learning and memory in young rats until two months post‐injection. This might be due to retained pNPC density and neuronal apoptosis in the DG of young rats after injection of hWJ‐MSC. In contrast, injection of hWJ‐MSC promoted both spatial learning and memory in aging rats, a finding that might be attributable to the increased pNPC density and attenuated neuronal apoptosis in DG of aging rats during the two months post‐injection. Our study suggests that a single injection of hWJ‐MSC might be sufficient to promote improvement in long‐term learning and memory in aging rats.

Stabilitas Fisikokimia dan Sifat Antipenuaan Kulit Formula Krim Berbahan Aktif Alami (Physicochemical stabilities and Skin Antiaging Properties of Bioaktive-based Cream Formulas) Rita K Sari; Nyoman J Wistara; Deded S Nawawi; Nopen Meisaroh; Ietje Wientarsih; D R Agungpriyono; L N Sutardi; Mawar Subangkit; Vetnizah Juniantito
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 15, No 1 (2017): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The purpose of this study was to analyze the physicochemical stabilities and in vivo antiaging properties of the cream formulas of F1, F2, and F3 which contained the active compound of 0.1% 0.18, and 0.36%, respectively. The active compound was the combination of ethyl acetate fraction of Toona sinensis, Centella asiatica, and Acacia mangium leaf (1:2:1). For physicochemical stability tests (color, odor, acidity, and antioxidant activity), the creams were stored for 21 days in the refrigerator, room temperature, and extreme temperature (±40 ⁰C). For in vivo antiaging test, the mice skin were smeared with cream and UV irradiated for 15 minutes per day. After two and four weeks, the mice skins were biopsied. The results showed that the physicochemical properties of creams stored in the refrigerator were stable. The storage of creams at room and extreme temperature decreased the physicochemical properties. The application of cream formulas on mice skin after two to four weeks were able to increase the thickness of epidermis, but has not been able to increase the collagen skin of mice.Keywords: Acacia mangium, Centella asiatica, ethyl acetate fraction of leaf, physicochemical and antiaging properties of cream, Toona sinensis

Aktivitas Antioksidan Ekstrak Daun Surian, Mangium, dan Pegagan serta Kombinasinya dalam Formula Krim (Antioxidant Activities of Mangium, Surian, and Pegagan Leaf Extracts and Its Combination in Cream Formulas) Rita K Sari; Nyoman J Wistara; Deded S Nawawi; Itje Wintarsih; Dewi R Agungpriyono; L N Sutardi; Mawar Subangkit; Vetnizah Juniantito
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 14, No 2 (2016): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The simplicia properties of toona (TS), mangium (AM), and centella (CA) leaves and antioxidant activities of their ethanolic leave extract and their combinations in cream formulas were examined in the present reserach. The ethanolic extracts were partitioned with n-hexane and ethyl acetate. The antioxidant activity of the fractions and their combinations were tested against DPPH. The best combination was formulated in F1, F2, and F3 cream formulas with 0.1, 0.18, and 0.36% of extract, respectively. The formulas antioxidant activity was then tested. The results showed that the water content, ash content, and acidic insoluble ash content of TS, AM, and CA simplicias were ±10%, 3.8-9.7%, and 0.9-1.2% respectively. The yields of n-hexane (FN), ethyl acetate (FEA), and residues (FR) fractions were 6-9, 2, and 5-6% respectively. The antioxidant activity of FEA, FR, and FN was very strong, strong, and weak to inactive, respectively. The best combination was the mixture of 2/3CA, 1/6AM, and 1/6TS (IC50 9 μg ml-1). The antioxidant activity of all cream formulas were higher than refered commercial cream. The IC50 values of formula 1, 2, and 3 were 1109, 596, and 202 μg ml-1, respectively, while the IC50 of refered commercial cream was 2372 μg ml-1.Keywords: antioxidant, centella, cream, extract, mangium, toona

Distribusi Penyebaran Bovine Viral Diarrhea Virus (BVDV) pada Sapi Potong Impor asal Australia Di Sukabumi Berdasarkan Uji Serologis Aditya Primawidyawan; Retno Wulansari; Surachmi Setiyaningsih; Mawar Subangkit; Bambang Pontjo Priosoeryanto
Acta VETERINARIA Indonesiana Vol. 11 No. 1 (2023): Maret 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.1.51-62

Virus Bovine Viral Diarrhea (BVDV) secara luas diakui memiliki dampak ekonomi yang signifikan pada ternak yang terinfeksi. Kerugian penyakit ini meliputi, hewan Persistenly Infection (PI) yang kurang sehat, penyakit reproduksi, penurunan produksi, pertumbuhan yang buruk, dan berpengaruh immunosupressif terhadap hewan ternak. Penelitian ini bertujuan untuk melakukan suatu kajian pola distribusi penyebaran dengan karakterisasi gejala klinis serta mendeteksi adanya kaitan pemeliharaan kandang sebagai faktor risiko sumber penularan penyakit BVD pada sapi potong impor. Jumlah hewan yang diambil 100 ekor sapi yang didapatkan dengan metode penghitungan kajian lintas seksional dengan pertimbangan penyakit ini sudah ada di Indonesia. Persebaran penyakit pada setiap kandang kandang A sampai H dengan total 80 ekor sapi terlihat sampel positif merata sebanyak 21 sampel pada posisi nilai S-N sebesar 0,3-0,7 di pengambilan awal dan meningkat pada nilai S-N sebesar 0,9-1,5 sebanyak 53 sampel. Peningkatan penyebaran pada kandang diluar kandang karantina mencapai 2,5x lipat mengindikasikan penyebaran BVDV cukup tinggi. Pengelolaan limbah buruk akan berpeluang 2,483 kali lebih besar menimbulkan hasil ELISA antigen positif bila dibandingkan dengan peternakan yang memiliki pengelolaan limbah yang baik (OR=2,483; CI=1,066-5,783). Program biosekuriti buruk akan berpeluang 2,667 kali lebih besar menimbulkan hasil ELISA Ag BVD positif dibandingkan dengan peternakan yang memiliki program biosekuriti yang baik (OR=2,667; CI=1,145-6,210). Bangsa Brahman Cross berpeluang tiga kali lebih besar memiliki peluang hasil positif bila dibandingkan dengan sapi yang memiliki ras non-Brahman Cross (OR=3; CI=1,269-7,091). Sedangkan umur sapi > dua tahun akan berpeluang 3,241 dibandingkan dengan umur sapi yang < dua tahun (OR= 3,241; CI=1,411-8,912). Impak studi penelitian terhadap jurnal ini adalah memberikan informasi distribusi penyebaran penyakit BVD asal dari hewan sapi impor dari Australia yang berguna sebagai pertimbangan tindakan preventif dalam upaya pengendalian penyakit.

ACUTE TOXICITY TEST OF ETHANOLIC EXTRACT OF Acalypha hispida LEAVES IN FEMALE RATS: A PHYSIOLOGICAL AND HISTOLOGICAL STUDY Hamzah Alfarisi; Mawar Subangkit; Siti Sa’diah; Tutik Wresdiyati
Jurnal Kedokteran Hewan Vol 14, No 3 (2020): September
Publisher : Universitas Syiah Kuala

This research aims to evaluate the safety of ethanolic extract of Acalypha hispida (A. hispida) leaves with acute toxicity test using 15 female rats strain Sprague-Dawley. A single dose of different doses of extract (2, 4, 8, and 16 g/kg body weight) was administrated orally, and theobservation was conducted for 14 days. The results revealed that the ethanolic extract of A. hispida leaves was relatively harmless (LD50 16 g/kg BW), did not affect body weight, and did not show clinical signs of toxicity during the observation periods. The parameters of blood serumbiochemistry of all extract-treated groups (alanine aminotransferase, aspartate aminotransferase, creatinine, and urea) did not change significantly compared to the control group. The histological observation of the liver showed a significant increase in eosinophilic cytoplasm and basophilic nuclei at all doses. However, the ethanolic extract of A. hispida leaves did not significantly affect glomerulus/Bowman’s capsule ratio, glomerular cell density, and the proportion of normal cell tubule. In conclusion, the ethanolic extract of A. hispida leaves was relatively harmless with LD5016 g/kg BW and seems to be safe in low doses (2 g/kg BW).

ACUTE TOXICITY TEST OF ETHANOLIC EXTRACT OF Acalypha hispida LEAVES IN FEMALE RATS: A PHYSIOLOGICAL AND HISTOLOGICAL STUDY Hamzah Alfarisi; Mawar Subangkit; Siti Sa’diah; Tutik Wresdiyati
Jurnal Kedokteran Hewan Vol 14, No 3 (2020): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i3.16176

This research aims to evaluate the safety of ethanolic extract of Acalypha hispida (A. hispida) leaves with acute toxicity test using 15 female rats strain Sprague-Dawley. A single dose of different doses of extract (2, 4, 8, and 16 g/kg body weight) was administrated orally, and theobservation was conducted for 14 days. The results revealed that the ethanolic extract of A. hispida leaves was relatively harmless (LD50 16 g/kg BW), did not affect body weight, and did not show clinical signs of toxicity during the observation periods. The parameters of blood serumbiochemistry of all extract-treated groups (alanine aminotransferase, aspartate aminotransferase, creatinine, and urea) did not change significantly compared to the control group. The histological observation of the liver showed a significant increase in eosinophilic cytoplasm and basophilic nuclei at all doses. However, the ethanolic extract of A. hispida leaves did not significantly affect glomerulus/Bowman’s capsule ratio, glomerular cell density, and the proportion of normal cell tubule. In conclusion, the ethanolic extract of A. hispida leaves was relatively harmless with LD5016 g/kg BW and seems to be safe in low doses (2 g/kg BW).

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