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INDEK GLIKEMIK UWI, GADUNG DAN TALAS YANG DIBERIKAN PADA TIKUS Sari, Ika Puspita; Lukitaningsih, Endang; Rumiyati, Rumiyati; Setiawan, Irfan Muris
Majalah Obat Tradisional Vol 18, No 3 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.68 KB) | DOI: 10.14499/mot-TradMedJ18iss3pp127-131

Abstract

Indonesia kaya akan umbi lokal yang semakin hari semakin dilupakan oleh generasi sekarang sebagai sumber bahan pangan yang sehat. Seiring dengan makin meningkatnya penyakit degeneratif terkait tingginya asupan glukosa, perlu digali kembali kekayaan lokal umbi-umbian dan pembuktian kandungan karbohidrat sehat di dalamnya yang dapat dimanfaatkan sebagai pangan di masa depan. Penelitian ini bertujuan untuk mengetahui indek glikemik (IG)  umbi-umbian yang diberikan secara oral pada tikus. Umbi yang diteliti antara lain, Uwi (Dioscorea alata), Gadung (Dioscorea hispida) dan Talas (Colocasia esculenta). Tikus dikelompokkan menjadi 9 kelompok masing-masing terdiri 4 ekor. Umbi diberikan dalam bentuk serbuk yang disuspensikan ke dalam Na CMC 0,1%  dengan dosis 2,5 g/kg berat badan (BB), kontrol positif berupa glukosa pemberian oral dosis 2,5 g/kg BB. Dilakukan pengambilan sampel darah hewan uji pada jam ke 0 (sebelum pemberian senyawa uji atau kontrol), 0,5; 1 dan jam ke-2 setelah pemberian senyawa uji.  Serum darah selanjutnya ditetapkan kadar glukosanya menggunakan GOD-PAP kit. Data berupa kadar glukosa darah hewan uji versus waktu dianalisis menjadi Area Under Curve (AUC) kadar glukosa versus waktu (0-2 jam). Perhitungan IG dilakukan dengan membandingkan AUC (0-2jam) senyawa uji terhadap AUC (0-2jam) glukosa. Hasil penelitian menunjukkan bahwa ketiga umbi yang diteliti memiliki nilai IG rendah (14-22). Umbi lokal dapat dikembangkan sebagai sumber karbohidrat yang sehat.
DETEKSI GAMMA AMINO BUTYRIC ACID (GABA) PADA DAUN Artocarpus altilis Indrowati, Meti; Astuti, Pudji; Pratiwi, Rarastoeti; Rumiyati, Rumiyati
Prosiding SNPS (Seminar Nasional Pendidikan Sains) 2015: Prosiding SNPS (Seminar Nasional Pendidikan Sains)
Publisher : Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (33.496 KB)

Abstract

Senyawa aktif Gamma Amino Butyric Acid (GABA) merupakan asam amino yang terdapat di otak mammalia dan berperan sebagai neurotransmiter.  Selain pada sistem saraf pusat, GABA juga terdapat dalam konsentrasi tinggi di sel β pankreas serta memiliki potensi sebagai antidiabetes. GABA dapat disintesis oleh hewan, bakteri asam laktat dan tumbuhan.  Penelitian dilakukan dengan tujuan mendeteksi keberadaan GABA dalam bagian tumbuhan yaitu daun Artocarpus altilis.  Deteksi dilakukan dengan Kromatografi Lapis Tipis (KLT) menggunakan fase diam plat silika, fase gerak BAW(3:1:1), dan GABA murni sebagai pembanding.  Larutan GABA dibuat dengan pelarut etanol:air (7:3).  Hasil penelitian menunjukkan GABA terdeteksi pada ekstrak daun Artocarpus altilis.
The Application of Geculan Bocah Dance in Improving Children’s Social Emotional Development Aged 5-6 Years in Pertiwi Kindergarten, Pakis Sub District, Magelang Regency Rumiyati, Rumiyati; Khamidun, Khamidun
BELIA: Early Childhood Education Papers Vol 6 No 2 (2017): November 2017
Publisher : Jurusan PGPAUD FIP UNNES

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Abstract

Children’s social emotional development aged 5-6 year in Permendikbud number 137 year2014 on national education early childhood standards there are level of achievement in child development (STPPA) PAUD 2013 curriculum which consists of self-awareness, a sense of responsibility for themselves and other people, and social behavior. Self-awareness covering on how to show the ability of themselves based on situation, shows prudence to the unknown people (then brought belief in a right adults), and knowing his own feelings and work them in (to exercise restraint in appropriately). A sense of responsibility of themselves and others covering on how she knows her right, obey the class rules (rules, activities), organize themselves, and responsible for her own doing for her own goodness. The act of pro-social is by playing with their peer, knowing others feeling and response it appropriately, sharing with others, appreciate others’ right/argument/and works, using acceptable norm in solving the problem (using their logical thinking in solving their problems), being cooperative with their friends, being tolerant, expressing their true feeling (happy, sad, enthusiastic etc), and acknowledge themselves with norms in their surroundings.   The Purpose of this study is to find an improvement in children’s social emotional development aged 5-6 years in kindergarten in Pertiwi kindergarten Pakis sub district Magelang regency through Geculan Bocah Dance treatment. A method of this research is experimental research with one group pretest posttest design. In this case treatment is given to children aged 5-6 years which consist of 33 children. Technique of analyzing data is using paired sample t-test. Children’s social emotional development aged 5-6 years in Pertiwi kindergarten Pakis sub district at first of 47,24 %. Then Geculan Bocah dance treatment was given. Children’s social emotional development aged 5-6 years in Pertiwi kindergarten Pakis sub district increased to 82,12 %.The size of the increase occurring of 34,88%. Conclusion that obtained in this research is: there has been increasing on children’s social emotional development aged 5-6 years in Pertiwi kindergarten Pakis sub district Magelang regency.
INDUCTION AND GROWTH KINETICS CALLUS OF TOMATO (SOLANUM LYCOPERSICUM) Setiaji, Arkan; Annisa, RR Rifka; Rumiyati, Rumiyati; Semiarti, Endang
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.21704

Abstract

Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ?Permata?) for optimum callus induction. Seeds were grown on ¼ MS medium for 10-15 days. Hypocotyl was excised and cultured on MS medium + 2 mg/l 2,4-D for 15 days as the explants for callus induction. Callus was transferred to MS medium with 8 variations of PGRs including the combination of BAP + NAA, and 2,4-D. Both fresh and dry weight was measured every 5 days over 60 days to establish the growth kinetics and growth efficiency of callus. Anatomic characters of calli were examined through paraffin-embedded method. The result showed of MS medium supplemented with 2.0 mg/l NAA and 0.2 mg/l BAP is optimum for tomato callus induction, based on highest number of the absolute growth rate on fresh weight (73.77% per day), dry weight (3.84% per day), and callus initiation time (5.56 days) achieved by the medium. Cells in the ground tissue of tomato hypocotyl are competent to be dedifferentiated into a callus. This research results were expected to find out suitable methods for tomato callus production in preparation for skincare uses.
The Application of Geculan Bocah Dance in Improving Children’s Social Emotional Development Aged 5-6 Years in Pertiwi Kindergarten, Pakis Sub District, Magelang Regency Rumiyati, Rumiyati; Khamidun, Khamidun
BELIA: Early Childhood Education Papers Vol 6 No 2 (2017): November 2017
Publisher : Jurusan PGPAUD FIP UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/belia.v6i2.17930

Abstract

Children’s social emotional development aged 5-6 year in Permendikbud number 137 year2014 on national education early childhood standards there are level of achievement in child development (STPPA) PAUD 2013 curriculum which consists of self-awareness, a sense of responsibility for themselves and other people, and social behavior. Self-awareness covering on how to show the ability of themselves based on situation, shows prudence to the unknown people (then brought belief in a right adults), and knowing his own feelings and work them in (to exercise restraint in appropriately). A sense of responsibility of themselves and others covering on how she knows her right, obey the class rules (rules, activities), organize themselves, and responsible for her own doing for her own goodness. The act of pro-social is by playing with their peer, knowing others feeling and response it appropriately, sharing with others, appreciate others’ right/argument/and works, using acceptable norm in solving the problem (using their logical thinking in solving their problems), being cooperative with their friends, being tolerant, expressing their true feeling (happy, sad, enthusiastic etc), and acknowledge themselves with norms in their surroundings. The Purpose of this study is to find an improvement in children’s social emotional development aged 5-6 years in kindergarten in Pertiwi kindergarten Pakis sub district Magelang regency through Geculan Bocah Dance treatment. A method of this research is experimental research with one group pretest posttest design. In this case treatment is given to children aged 5-6 years which consist of 33 children. Technique of analyzing data is using paired sample t-test. Children’s social emotional development aged 5-6 years in Pertiwi kindergarten Pakis sub district at first of 47,24 %. Then Geculan Bocah dance treatment was given. Children’s social emotional development aged 5-6 years in Pertiwi kindergarten Pakis sub district increased to 82,12 %.The size of the increase occurring of 34,88%. Conclusion that obtained in this research is: there has been increasing on children’s social emotional development aged 5-6 years in Pertiwi kindergarten Pakis sub district Magelang regency.
Validasi Metode Real-Time Polymerase Chain Reaction untuk Deteksi DNA Babi (Sus Scrofa Domestica) dan Celeng (Sus Barbatus) pada Sosis Sapi Mariyani, Mariyani; Sismindari, Sismindari; Rumiyati, Rumiyati
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : CV. Ridwan Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.369 KB) | DOI: 10.36418/syntax-literate.v6i8.3806

Abstract

Babi (Sus scrofa domestica) dan celeng (Sus barbatus) hewan satu spesies yang memiliki kemiripan sangat tinggi, perbedaan keduanya dilihat dari susunan basa DNA yang dimiliki. Metode analisis DNA antara babi dan celeng banyak yang belum berhasil membedakan kedua spesies tersebut seperti pada penelitian yang dilakukan Hikmah (2019) untuk membedakan babi dan celeng menggunakan primer Nk-ND1 dengan metode real time PCR namun berhasil mendapatkan perbedaan basa antara fragmen DNA babi dan celeng dari hasil sekuensing sehingga perbedaan urutan basa tersebut dijadikan sebagai primer spesifik babi untuk membedakan kedua spesies babi dan celeng dengan metode real time PCR. Lima spesies hewan yang digunakan yaitu babi, celeng, sapi, kambing dan ayam, primer babi forward 5’-GATGCCCTAAAACTATTCACC-3’ dari hasil sekuensing dan primer babi reverse yaitu 5’-TAGTGCTAGGGATAAGGCTAGG-3’ desain Hikmah (2019), SsoFast™ EvaGreen® Supermix (Bio-Rad), proteinase-K (Invitrogen), isolasi DNA menggunakan Geneaid Genomic DNA Mini Kit. Validasi metode real time PCR dengan uji spesifitas primer terhadap 5 isolat DNA jaringan segar, uji efisiensi dan sensitivitas pada 6 konsentrasi DNA (50; 5; 0,5; 0,05; 0,005 dan 0,0005 ng) dan sosis referensi campuran daging sapi:babi (100%; 50%; 25%; 10%; 5% dan 1%), uji keterulangan dilakukan sebanyak 5 kali replikasi. Hasil analisis menunjukkan sepasang primer spesifik terhadap DNA babi dan celeng. Efisiensi DNA babi yaitu 109,5% dan R2 = 0,967, efisiensi celeng 99,1% dan R2 = 0,999. Batas deteksi absolut DNA babi dan celeng yaitu 0,05 ng/µL dan 0,5 ng/µL, batas deteksi relatif sosis babi yaitu 5% dan sosis celeng 1%. Nilai RSD rata-rata pada analisis keterulangan DNA babi dan celeng yaitu 4,210% dan 3,611%. Pada sampel tidak terdteeksi adanya DNA babi dan celeng, secara kuantitatif metode ini tidak memenuhi persyaratan validasi.