Claim Missing Document
Check
Articles

Found 19 Documents
Search

Analisis Kuantitatif 15-F2t-isoprostan dari Plasma Pasien Obstructive Sleep Apnea (OSA) dengan Metode Enzyme Linked Immunosorbent Assay (ELISA) menggunakan Teknik Ekstraksi Imunoafinitas Rusdi, Bertha; Gusdinar, Tutus; Fattah, Miswar
Indonesian Journal of Clinical Pharmacy Vol 1, No 1 (2012)
Publisher : Indonesian Journal of Clinical Pharmacy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (792.299 KB)

Abstract

15-F2t-isoprostan merupakan penanda stres oksidatif yang kadarnya dalam cairan biologis relatif rendah serta memiliki banyak isomer sehingga diperlukan ekstraksi sampel sebelum dilakukan pengukuran kadar. Metode ekstraksi yang digunakan diantaranya ekstraksi fase solid/Solid Phase Extraction (SPE) serta ekstraksi imunoafinitas. Perbaikan teknik ekstraksi SPE dan teknik ekstraksi imunoafinitas dilakukan untuk membandingkan hasil perolehan kembalinya. Pengukuran dilakukan dengan metode Enzyme Linked Immunosorbent Assay (ELISA). Penilaian efektivitas proses ekstraksi diamati melalui hasil perolehan kembali dari kedua teknik ekstraksi. Teknik ekstraksi dengan perolehan kembali tertinggi digunakan untuk mengukur kadar 15-F2t-isoprostan dari penderita Obstructive Sleep Apnea (OSA). Teknik ekstraksi imunoafinitas menghasilkan perolehan kembali 15-F2t-isoprostan yang cukup baik. Pada penderita OSA kadar 15-F2t-isoprostan dalam plasma cenderung tinggi sehingga memiliki risiko untuk menderita penyakit yang berhubungan dengan aktivitas biologis 15-F2t-isoprostan seperti arteriosklerosis.Kata kunci: 15-F2t-isoprostan, Solid Phase Extraction (SPE), ekstraksi imunoafinitas, ObstructiveSleep Apnea (OSA) Quantitative Analysis of Free 15-F2t-Isoprostane from Plasma of Obstructive Sleep Apnea Patients Using Enzyme Linked Immunosorbent Assay Method with Immunoaffinity Extraction TechniqueAbstract15-F2t-isoprostane is a biomarker in assessment of oxidative stress status that due to its relatively low concentration in biological fluid and also has many isomers, the 15-F2t-isoprostane sample need to be extracted prior to the quantifying processes. Extraction techniques commonly used to extract 15-F2t-isoprostane are solid phase extraction (SPE) and immunoaffinity extraction. Improvements to the SPE and immunoaffinity extraction techniques had been conducted, and the recovery results was then compared. The quantification of 15-F2t-isoprostane then was conducted using Enzyme Linked Immunosorbent Assay (ELISA) method. Then followed by the examination of the plasma recovery results. Extraction technique which had the highest recovery then was used to quantify 15-F2t-isoprostane from plasma of Obstructive Sleep Apnea (OSA) patients. Immunoaffinity extraction technique has a good recovery result. OSA patients have the tendency to have high 15-F2t-isoprostane concentrations in the plasma, therefore have a potential risk to get diseases related to the biological activities of 15-F2t-isoprostane, such as arteriosclerosis.Key words: 15-F2t-isoprostane, Solid Phase Extraction (SPE), immunoaffinity extraction, ObstructiveSleep Apnea
Analisis Kualitas Tepung Ampas Tahu Rusdi, Bertha; Maulana, Indra Topik; Kodir, Reza Abdul
Jurnal Matematika dan Sains Vol 18 No 2 (2013)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Analisis kualitas tepung ampas tahu yang meliputi parameter kualitas umum telah dilakukan. Parameter kualitas yang dianalisis antara lain kadar abu, nutrisi, dan cemaran. Nutrisi meliputi kadar protein dan karbohidrat, sedangkan analisis cemaran meliputi logam berat, yakni Pb dan Cd, mikroba (Bacillus cereus, Salmonella sp. E.coli), dan angka lempeng total (ALT). Tepung ampas tahu divariasikan menjadi dua, yaitu melalui pencucian dan tanpa pencucian. Hasil olahan tepung ampas tahu tanpa pencucian memiliki kandungan nutrisi yang lebih tinggi dan cemaran yang lebih rendah dibandingkan dengan yang terlebih dahulu melalui proses pencucian. Tepung ampas tahu tanpa pencucian selanjutnya diolah menjadi kue brownies, di mana brownies yang dihasilkan memiliki penampilan dan rasa yang cukup baik. Kata kunci: tepung ampas tahu, nutrisi, cemaran   Quality analysis of Tofu Waste Flour Abstract Quality analysis of Tofu waste flour has been conducted, including ash level, nutrition, and contaminat. Nutrition which was analyzed were protein and carbohydrate concentration. And contaminat analyze including heavy metal contaminant (Pb and Cd) and microbiological contaminant (Bacillus cereus, Salmonella sp., E. Coli and total plate count). Unwashed tofu waste flour has higher nutrition value and less contaminant. Tofu waste flour can be used in brownies product. Brownies from this flour has a good flavor and appearance. Key words: tofu waste, nutrition, contaminant
Analisis Kuantitatif 15-F2t-isoprostan dari Plasma Pasien Obstructive Sleep Apnea (OSA) dengan Metode Enzyme Linked Immunosorbent Assay (ELISA) menggunakan Teknik Ekstraksi Imunoafinitas Bertha Rusdi; Tutus Gusdinar; Miswar Fattah
Indonesian Journal of Clinical Pharmacy Vol 1, No 1 (2012)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (792.299 KB)

Abstract

15-F2t-isoprostan merupakan penanda stres oksidatif yang kadarnya dalam cairan biologis relatif rendah serta memiliki banyak isomer sehingga diperlukan ekstraksi sampel sebelum dilakukan pengukuran kadar. Metode ekstraksi yang digunakan diantaranya ekstraksi fase solid/Solid Phase Extraction (SPE) serta ekstraksi imunoafinitas. Perbaikan teknik ekstraksi SPE dan teknik ekstraksi imunoafinitas dilakukan untuk membandingkan hasil perolehan kembalinya. Pengukuran dilakukan dengan metode Enzyme Linked Immunosorbent Assay (ELISA). Penilaian efektivitas proses ekstraksi diamati melalui hasil perolehan kembali dari kedua teknik ekstraksi. Teknik ekstraksi dengan perolehan kembali tertinggi digunakan untuk mengukur kadar 15-F2t-isoprostan dari penderita Obstructive Sleep Apnea (OSA). Teknik ekstraksi imunoafinitas menghasilkan perolehan kembali 15-F2t-isoprostan yang cukup baik. Pada penderita OSA kadar 15-F2t-isoprostan dalam plasma cenderung tinggi sehingga memiliki risiko untuk menderita penyakit yang berhubungan dengan aktivitas biologis 15-F2t-isoprostan seperti arteriosklerosis.Kata kunci: 15-F2t-isoprostan, Solid Phase Extraction (SPE), ekstraksi imunoafinitas, ObstructiveSleep Apnea (OSA) Quantitative Analysis of Free 15-F2t-Isoprostane from Plasma of Obstructive Sleep Apnea Patients Using Enzyme Linked Immunosorbent Assay Method with Immunoaffinity Extraction TechniqueAbstract15-F2t-isoprostane is a biomarker in assessment of oxidative stress status that due to its relatively low concentration in biological fluid and also has many isomers, the 15-F2t-isoprostane sample need to be extracted prior to the quantifying processes. Extraction techniques commonly used to extract 15-F2t-isoprostane are solid phase extraction (SPE) and immunoaffinity extraction. Improvements to the SPE and immunoaffinity extraction techniques had been conducted, and the recovery results was then compared. The quantification of 15-F2t-isoprostane then was conducted using Enzyme Linked Immunosorbent Assay (ELISA) method. Then followed by the examination of the plasma recovery results. Extraction technique which had the highest recovery then was used to quantify 15-F2t-isoprostane from plasma of Obstructive Sleep Apnea (OSA) patients. Immunoaffinity extraction technique has a good recovery result. OSA patients have the tendency to have high 15-F2t-isoprostane concentrations in the plasma, therefore have a potential risk to get diseases related to the biological activities of 15-F2t-isoprostane, such as arteriosclerosis.Key words: 15-F2t-isoprostane, Solid Phase Extraction (SPE), immunoaffinity extraction, ObstructiveSleep Apnea
Aktivitas Jus Jeruk Nipis pada Mencit Jantan Swiss Webster yang Diinduksi Prokarbazin Hidroklorida Suwendar Suwendar; Bertha Rusdi; Kiki Mulkiya Yuliawati
MIMBAR (Jurnal Sosial dan Pembangunan) Volume 24, No. 1, Year 2008
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (778.25 KB) | DOI: 10.29313/mimbar.v24i1.253

Abstract

Anti thrombocytopenia activity of the Citrus aurantifolia fruit juice using direct counting methods on adrenaline-induced mice has been carried out.  The induction effect tests of chloramphenicol, phenylbutazone and adrenaline were studied.  Results showed that adrenaline at the dosage of 0.08 mg/ kg body weight given intravenously, gave thrombocytopenia effect after 9 and 12 minutes. Anti thrombocytopenia effect of the juice of Citrus  aurantifolia fruit given orally into mice induced by adrenaline has been studied. Results showed that the juice of Citrus aurantifolia fruit at the dosage of  18 ml / kg of  body weight during seven days increased the thrombocyte count significantly (P<0.05). The juice at 9 and 4,5 ml / kg of body weight inhibited the decrease of trombocyte count.
Studi Literatur Senyawa Aktif Antibakteri dari Ekstrak Daun Salam Koja (Murraya koenigii (Linn) Spreng) Muhammad Khalid Anshari; Bertha Rusdi
Jurnal Riset Farmasi Volume 1, No. 2, Desember 2021, Jurnal Riset Farmasi (JRF)
Publisher : UPT Publikasi Ilmiah Unisba

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (694.86 KB) | DOI: 10.29313/jrf.v1i2.571

Abstract

Abstract. Koja bay leaves (Murraya koenigii (Linn) Spreng) which are widely found in Indonesia are scientifically proven to have antibacterial effects. In this study, a Systematic Literature Review was conducted on the antibacterial effect of bay leaf koja so that it is known that bay leaf extract has antibacterial activity against Gram positive and Gram negative bacteria, the value of Minimum Inhibition Concentratiton (MIC) against these bacteria and the antibacterial compounds contained therein. From the results of a search of 17 journals published in the last 10 years, it is known that koja bay leaf extract can inhibit Gram-positive bacteria among others Bacillus cereus, Bacillus megaterium, Bacillus subtilis, Staphylococcus aureus, Staphylococcus subfava, Staphylococcus epidermidis, Staphylococcus subfava, Streptococcus mutans, Lactobacillus acidophilus, Lactobacillus casie, and Gram-negative bacteria among others Alcaligenesfecalis, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella abony, dan Salmonella thyphi with MIC values between 0,090 to 175 mg/mL. Bay leaf extracts that have antibacterial activity are extracts of water, ethanol, petroleum ether, hexane, chloroform, ethyl acetate, and methanol. Chemical compounds that have antibacterial activity in bay leaf extract include acetophenone, alkaloid, carbazole alkaloid, crystalline, girinimbine, iso mahanimbine, koenidine, koenimbine, koenine, mahanimbine, mahanimbicine, dan mahanine. Abstrak. Daun salam koja (Murraya koenigii (Linn) Spreng) yang banyak ditemukan di Indonesia secara ilmiah terbukti memiliki efek antibakteri. Pada penelitian ini dilakukan Systematic Literature Review mengenai efek antibakteri dari daun salam koja sehingga diketahui ekstrak daun salam koja memiliki aktivitas antibakteri terhadap bakteri Gram positif dan Gram negatif, nilai Minimum Inhibition Concentration (MIC) terhadap bakteri tersebut, dan senyawa yang memiliki aktivitas antibakteri di dalam ekstrak daun salam koja. Dari hasil penelusuran terhadap 17 jurnal yang diterbitkan dalam kurun waktu 10 tahun terakhir menyatakan bahwa ekstrak daun salam koja dapat menghambat bakteri Gram positif antara lain Bacillus cereus, Bacillus megaterium, Bacillus subtilis, Staphylococcus aureus, Staphylococcus subfava, Staphylococcus epidermidis, Staphylococcus subfava, Streptococcus mutans, Lactobacillus acidophilus, Lactobacillus casie, dan dapat menghambat bakteri Gram negatif antara lain Alcaligenesfecalis, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella abony, dan Salmonella thyphi dengan nilai MIC yang diperoleh antara 0,090 hingga 175 mg/mL. Ekstrak daun salam koja yang memiliki aktivitas antibakteri adalah ekstrak air, etanol, petroleum eter, heksana, kloroform, etil asetat, dan metanol. Senyawa kimia yang memiliki aktivitas antibakteri di dalam ekstrak daun salam koja meliputi acetophenone, carbazole alkaloid, crystalline, girinimbine, iso mahanimbine, koenidine, koenimbine, koenine, mahanimbine, mahanimbicine, dan mahanine.
Studi Bioinformatika Mutasi Genetik pada Gen Pengkode Protein Spike dari Virus Sars-Cov-2 di Indonesia Muhammad Ilham Hardian; Bertha Rusdi
Bandung Conference Series: Pharmacy Vol. 1 No. 1 (2021): Bandung Conference Series: Pharmacy
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.524 KB) | DOI: 10.29313/bcsp.v1i1.89

Abstract

Abstract. SARS-CoV-2 is a new type of virus that causes the current pandemic. Corona virus gene mutations have become popular since the discovery of SARS-CoV-2 variants in the UK (B.1.1.7), South Africa (B.1.351), India (B.1.617.1 and B.1.617.2). The purpose of this study was to analyze the genetic mutations of L452R and N501Y in SARS-CoV-2 virus isolates found in Indonesia to obtain an overview of their role in the ACE2 receptor binding site. The benefits of this research are expected to provide scientific information regarding genetic mutations of SARS-CoV-2 isolates that are useful for vaccine development and various other studies. A bioinformatic study that began with the search for the sequence of genes encoding the SARSCoV2 protein S that had mutations from Indonesian patient isolates on the Global Initiative on Sharing All Influenza Data (GISAID) page. Furthermore, the SARSCoV2 genome sequence that was first reported in Wuhan (Wuhan-1) was extracted from the National Center for Biotechnology Information (NCBI) page for comparison. The sequence of the gene encoding the protein S that had been obtained by GISAID was compared to the sequence of the gene encoding the protein S of the Wuhan-1 isolate using the Clustal Omega software. To compare the amino acid sequences of these isolates, the Coding Sequences (CDS) of protein S of the wuhan-1 isolate were translated into amino acid sequences using Skaminsky115. The nucleotide and amino acid sequences of protein S that have been obtained are then analyzed to map the position of RBD related to the mutation that occurs. The results of the study are genomic data that have been carried out by aligning the amino acid sequences of several Indonesian isolates and Wuhan-Hu-1 isolates. Then found mutations in the sequence of DNA bases and amino acid bases that are thought to be in the area around the RBD, namely in the order of (471, 472, 478, 480, 534). Abstrak. SARS-CoV-2 merupakan jenis virus baru penyebab pandemi saat ini. Mutasi gen virus corona menjadi populer semenjak ditemukannya varian SARS-CoV-2 di Inggris (B.1.1.7), Afrika Selatan (B.1.351), India (B.1.617.1 dan B.1.617.2). Tujuan dari penelitian ini adalah menganalisis mutasi genetik L452R dan N501Y pada isolat virus SARS-CoV-2 yang ditemukan di Indonesia untuk mendapatkan gambaran terhadap perannya pada daerah pengikatan reseptor ACE2. Manfaat penelitian ini diharapkan dapat memberikan informasi ilmiah mengenai mutasi genetik isolat SARS-CoV-2 yang berguna untuk pengembangan vaksin dan berbagai penelitian lainnya. Studi bioinformatik yang diawali dengan pencarian urutan gen pengkode protein S SARSCoV2 yang mengalami mutasi dari isolat pasien Indonesia pada laman Global Initiative on Sharing All Influenza Data (GISAID). Selanjutnya urutan genom SARSCoV2 yang dilaporkan pertama kali di Wuhan (Wuhan-1) diekstrak dari laman National Center for Biotechnology Information (NCBI) untuk dijadikan pembanding. Urutan gen pengkode protein S yang telah diperoleh dr GISAID dibandingkan terhadap urutan gen pengkode protein S isolat Wuhan-1 menggunakan perangkat lunak Clustal Omega. Untuk membandingkan urutan asam amino isolat-isolat tersebut, Coding Sequens (CDS) protein S isolat wuhan-1 diterjemahkan menjadi urutan asam amino menggunakan Skaminsky115. Urutan nukleotida dan asam amino protein S yang telah diperoleh kemudian dianalisis untuk memetakan posisi RBD berkaitan dengan mutasi yg terjadi. Hasil penelitian berupa data genomik yang telah dilakukan penyejajaran urutan asam amino dari beberapa isolat Indonesia dan isolat Wuhan-Hu-1. Kemudian ditemukan mutasi pada urutan basa DNA dan basa asam amino yang diduga berada pada daerah sekitar RBD yaitu pada urutan ke-(471, 472, 478, 480, 534).
Studi Literatur Perbandingan Sensitivitas dan Spesifisitas Berbagai Metode Deteksi Virus SARS-CoV-2 Reisya Nabila; Hilda Aprilia; Bertha Rusdi
Bandung Conference Series: Pharmacy Vol. 2 No. 2 (2022): Bandung Conference Series: Pharmacy
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (499.945 KB) | DOI: 10.29313/bcsp.v2i2.3956

Abstract

Abstract. SARS-CoV-2 is a virus that causes COVID-19 disease, this disease has become an epidemic and quickly transmitted disease and has even mutated into several variants. This is the main idea for the need of a detection methods that can detect viruses properly. This study aims to find out: (1) what are the methods that can be used to detect the SARS-CoV-2 virus? (2) what are the advantages and disadvantages of the SARS-CoV-2 virus detection method that has been developed in terms of sensitivity and specificity? (3) what methods can be used to detect the new SARS-CoV-2 genetic variants?. The method used is a systematic literature by reviewing several journals by taking a research data from official website of Science Direct and PubMed publications using the keywords “COVID-19”, “SARS-CoV-2 detection”, “detection of COVID-19”, “SARS-CoV-2 new method detection sensitivity and specificity”. Furthermore, the selection of journals is based on several inclusion and exclusion criteria. Based on the results of research on 9 journals, the SARS-CoV-2 virus can be detected using the RT-PCR, CRISPR-top, LAMP, ELISA, LFA, LEAD, Serology/ Rapid, GeNose, and Colorimetric Sensors methods. Good sensitivity and specificity values exist in the RT-PCR method (97.3%/97.5%), LAMP (87.0%/ 98.5%) but this method is more specific, and ELISA (94.5% /99.4%). The RT-PCR method remains the gold standart for the methods used to detect SARS-CoV-2 virus sensitively and specifically, while the LAMP method can be detect specific Omicron variants. Abstrak. SARS-CoV-2 adalah virus penyebab penyakit COVID-19, penyakit ini telah menjadi epidemi dan penyakit yang menular dengan cepat bahkan telah bermutasi menjadi beberapa varian. Hal inilah yang menjadi pokok pemikiran perlunya suatu metode deteksi yang dapat mendeteksi virus dengan baik. Penelitian ini bertujuan untuk mengetahui: (1) Apa saja metode yang dapat digunakan untuk mendeteksi virus SARS-CoV-2? (2) Apa kelebihan dan kekurangan metode deteksi virus SARS-CoV-2 yang telah dikembangkan dilihat dari sensitivitas dan spesifisitas? (3) Apa metode yang dapat digunakan untuk mendeteksi varian genetik SARS-CoV-2 terbaru?. Metode yang digunakan adalah sistematika literatur dengan me-review beberapa jurnal dan mengambil data penelitian melalui website resmi publikasi Science Direct dan PubMed menggunakan kata kunci “COVID-19”, “deteksi SARS-CoV-2”, “deteksi COVID-19”, “sensitivitas dan spesifisitas metode deteksi baru SARS-CoV-2”. Selanjutnya, pemilihan jurnal didasarkan pada beberapa kriteria inklusi dan eksklusi. Berdasarkan hasil penelitian pada 9 jurnal, virus SARS-CoV-2 dapat dideteksi menggunakan metode RT-PCR, CRISPR-top, LAMP, ELISA, LFA, LEAD, Serology/Rapid, GeNose, dan Colorimetric Sensors. Nilai sensitivitas dan spesifisitas yang baik terdapat pada metode RT-PCR (97,3%/97.5%), LAMP (87,0%/ 98,5%) namun metode ini lebih spesifik, dan ELISA (94.5%/99.4%). Metode RT-PCR tetap menjadi standar emas untuk metode yang digunakan untuk mendeteksi virus SARS-CoV-2 secara sensitif dan spesifik, sedangkan metode LAMP dapat mendeteksi varian Omicron secara spesifik COVID-19, deteksi SARS-CoV-2, deteksi COVID-19, sensitivitas dan spesifisitas metode deteksi baru SARS-CoV-2.
Studi Literatur Potensi Tanaman Jintan Hitam (Nigella sativa Linn.) dalam Penanganan Demensia Aulia Lairanisa; Bertha Rusdi
Bandung Conference Series: Pharmacy Vol. 2 No. 2 (2022): Bandung Conference Series: Pharmacy
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.49 KB) | DOI: 10.29313/bcsp.v2i2.3961

Abstract

Abstract. Dementia is a syndrome of severe intellectual decline that can interfere with a person's daily activities. Until now, drugs that can cure dementia have not been found, while the existing dementia therapy generally uses drugs with an acetylcholinesterase inhibitor mechanism that can cause unwanted cholinergic effects. Therefore, it is necessary to explore more about other sources of drugs that are expected to have better aspects of safety, efficacy, and quality. One of them is black cumin. Based on research journals and available empirical information, black cumin has potential as an alternative strategy for the treatment of dementia. Therefore, a literature study was conducted on the potential of black cumin plants in the treatment of dementia. There were 15 research journals, consisting of 13 research journals based on in vivo, as well as 2 in vitro research journals. The conclusion obtained is that the compounds contained in black cumin have the potential to treat dementia with thymoquinone (TQ) as the main active compound. In general, black cumin can reduce adverse effects significantly through anti-inflammatory mechanisms, decrease levels of amyloid protein which is one of the causes of Alzheimer's dementia, as well as a neuroprotector. Abstrak. Demensia merupakan sindrom penurunan fungsi intelektual yang cukup berat yang dapat mengganggu aktivitas keseharian seseorang. Hingga saat ini, obat yang mampu menyembuhkan demensia belum ditemukan, sedangkan terapi demensia yang ada umumnya menggunakan obat dengan mekanisme penghambat asetilkolinesterase yang dapat menyebabkan efek kolinergik yang tidak diinginkan. Maka perlu untuk menggali lebih mengenai sumber obat lain yang diharap memiliki aspek keamanan, khasiat, serta kualitas yang lebih baik. Salah satunya adalah jintan hitam (Nigella sativa Linn.). Berdasarkan jurnal penelitian serta informasi empiris yang ada, jintan hitam memiliki potensi sebagai strategi alternatif untuk pengobatan demensia. Maka dari itu, dilakukan studi literatur potensi tanaman jintan hitam dalam penanganan demensia. Terdapat 15 jurnal yang diteliti, terdiri atas 13 jurnal penelitian berdasarkan in vivo, serta 2 jurnal penelitian in vitro. Kesimpulan yang didapatkan adalah senyawa yang terkandung dalam jintan hitam memiliki potensi dalam penangan demensia dengan timokuinon (TQ) sebagai senyawa aktif utamanya. Secara umum, jintan hitam dapat menurunkan efek merugikan secara signifikan melalui mekanisme antiinflamasi, penurunan kadar protein amiloid yang merupakan salah satu penyebab demensia alzheimer, serta sebagai neuroprotektor.
Kajian Pustaka Potensi Kulit Nanas (Ananas comosus (L.) Merr) sebagai Bahan Baku Pembuatan Bioetanol Fika Nurul Hafidzoh; Bertha Rusdi; Kiki Mulkiya Yuliawati
Bandung Conference Series: Pharmacy Vol. 2 No. 2 (2022): Bandung Conference Series: Pharmacy
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (642.566 KB) | DOI: 10.29313/bcsp.v2i2.4425

Abstract

Abstract. One type of fruit that is widely cultivated in Indonesia is pineapple. Pineapple fruit that is produced produces a high enough waste, and if it is not used it will cause an unpleasant odor due to the process of decay. Pineapple peel is one of the agricultural wastes that can produce bioethanol because it contains carbohydrates such as reducing sugars. The presence of high carbohydrate and sugar content is converted into bioethanol through a fermentation process with the help of microorganisms. The purpose of this study was to determine how the mechanism of making bioethanol from pineapple peels, to find out what factors influence the levels of bioethanol produced from pineapple peel fermentation, and to find out what the optimal conditions are to obtain the highest levels of bioethanol from pineapple peels.This study uses a systematic literature review method with stages, namely library research, screening based on inclusion and exclusion criteria and data extraction from 6 journals. The results of this study indicate that the mechanism for making bioethanol starts from the process of hydrolysis, fermentation, then distillation. The factors that influence the fermentation process are the type and number of microbes used, length of fermentation time, pH, additives, and temperature. Among the six journals, the highest bioethanol content obtained was 38%, using Saccharomyces cerevisiae mobilized in alginate granules with a fermentation time of 4 days, and 14 grams of yeast used. Abstrak. Salah satu jenis buah-buahan yang banyak dibudidayakan di Indonesia yaitu buah nanas. Buah nanas yang diproduksi menghasilkan limbah yang cukup tinggi, dan jika tidak dimanfaatkan maka akan menimbulkan bau yang tidak sedap karena terjadi proses pembusukan. Kulit nanas merupakan salah satu limbah pertanian yang dapat menghasilkan bioetanol karena mengandung karbohidrat seperti gula reduksi. Adanya kandungan karbohidrat dan gula yang cukup tinggi diubah menjadi bioetanol melalui proses fermentasi dengan bantuan mikroorganisme. Tujuan dari penelitian ini untuk mengetahui bagaimana mekanisme pembuatan bioetanol daari kulit nanas, untuk mengetahui apa saja faktor yang mempengaruhi kadar bioetanol yang dihasilkan dari fermentasi kulit nanas, serta untuk mengetahui bagaimana kondisi optimal untuk memperoleh bioetanol dengan kadar tertinggi dari kulit nanas. Penelitian ini menggunakan metode kajian pustaka sistematis dengan tahapan yaitu penelusuran pustaka, penyaringan berdasarkan kriteria inklusi dan eksklusi serta ekstraksi data dari 6 jurnal. Hasil penelitian ini menunjukkan bahwa mekanisme pembuatan bioetanol dimulai dari proses hidrolisis, fermentasi, kemudian destilasi.Faktor-faktor yang mempengaruhi proses fermentasi adalah jenis dan jumlah mikroba yang digunakan, lamanya waktu fermentasi, pH, zat tambahan, dan suhu. Diantara keenam jurnal kadar bioetanol tertinggi yang diperoleh yaitu sebesar 38%, menggunakan Saccharomyces cerevisiae termobilisasi dalam butiran alginat dengan waktu fermentasi selama 4 hari, dan khamir yang digunakan sebnayak 14 gram.
Studi Literatur Ekstraksi Pekti Dari Kulit Buah Naga (Hylocereus polyrhizus) Marwah; Bertha Rusdi; Diar Herawati
Bandung Conference Series: Pharmacy Vol. 2 No. 2 (2022): Bandung Conference Series: Pharmacy
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (594.262 KB) | DOI: 10.29313/bcsp.v2i2.4696

Abstract

Abstract. Pectin is a natural substance found in most plants that acts as an adhesive and maintains tissue stability. This study aims to determine the pectin extraction metodh that can be used to extract pectin from dragon fruir feel by producing an optimum yield, and to determine the advantages and disadvantages of the dragon fruit peel pectin extraction metodh. In this study, a literature review study was carried out which was taken from several research journals that had extracted pectin from dragon fruit peel using several extraction metodhs, namely conventional, microwave, and ultrasonic extraction. The results obtained from this literature review study are that using the microwave extraction metodh is able to extact pectin from dragon fruit peel by giving a higher yield of about 23%, using a small amount of solvent and using a very short extraction time. Abstrak. Pektin merupakan substansi alami yang terdapat pada sebagian besar tanaman yang berperan sebagai perekat dan menjaga stabilitas jaringan. Penelitian ini bertujuan untuk mengetahui metode ekstraksi pektin yang dapat digunakan untuk mengekstraksi pektin dari kulit buah naga dengan menghasilkan rendemen yang optimum, serta mengetahui kelebihan dan kekurangan dari metode ekstraksi pekti kulit buah naga tersebut. Pada penelitian ini dilakukan secara studi literatur review yang diambil dari beberapa jurnal penelitian yang telah melakukan ekstraksi pektin dari kulit buah naga dengan menggunakan beberapa metode ekstraksi yaitu ekstraksi konvensional, microwave dan ultrasonic. Hasil yang diperoleh dari studi literatur review ini adalah dengan menggunakan metode ekstraksi microwave mampu mengekstraksi pektin dari kulit buah naga dengan memberikan hasi rendemen yang lebih banyak sekitar 23%, dengan menggunakan jumlah pelarut yang sedikit dan menggunakan waktu ekstraksi yang sangat singkat.