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Sri Nopita Primawati
Program Studi Pendidikan Biologi FPMIPA IKIP Mataram Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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EFEKTIVITAS SENYAWA BIOAKTIF EKSTRAK KENCUR (KAEMPFERIA GALANGA L.) MENGGUNAKAN PELARUT AQUADES DENGAN PELARUT METANOL Sri Nopita Primawati; Nofisulastri Nofisulastri; Baiq Asma Nufida
Bioscientist : Jurnal Ilmiah Biologi Vol 1, No 2 (2013): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bjib.v1i2.788

Abstract

Kaempferia galanga L. capability as antibacterial has been proven by the formation of drag zone in some pathogenic bacteria such as Staphylococcus aureus, Streptococcus pyogenes and Klebsiella pneumonia by disc-diffusion method. The secondary metabolite content of Kaempferia galanga L., which is efficacious as antibacterial include essential oil, sesquiterpenoid, flavonoid, phenolic or polyfenol compounds and alkaloids. Therefore, it is necessary to do this research to isolate antibacterial compounds from kencur with various solvents such as aquadest with methanol. This research was a laboratory experiment with complete randomized design (RAL) with extract concentration of 50%, 75% and 100% of aquadest solvent with methanol five times repetition. Antibacterial test using agar diffusion method with wells. The inhibit zone formed is tabulated and analyzed using one-way analysis of variance (Anova). It was found that there was a significant difference between the extract with the aquadest solvent and the methanol solvent extract on Salmonella typhimurium growth at a significant level of 5% (Fcount (282.86) > Ftable (2.62)). However the drag zone formed in the weak category is below 15 mm. So it can be concluded that the extract methanol kencur showed weak antibacterial activity seen from the increase in diameter of inhibit zone of Salmonella typhimurium bacteria significantly along with increasing the concentration of methanol extract of kencur rhizome but not on the extract of aquadest Kaempferia galanga L. kale rhizome.
STUDI KEBERADAAN SENYAWA BIOAKTIF IMUNOSUPRESAN PADA EKSTRAK TESTIS SAPI BALI (Bos sondaicus) MELALUI UJI IN VITRO Sri Nopita Primawati
Bioscientist : Jurnal Ilmiah Biologi Vol 1, No 1 (2013): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bjib.v1i1.775

Abstract

A study of the existence of Bioactive Immunosuppressant Compounds on Balinese Testis Extract (Bos sondaicus) through In Vitro Test. This study aims to study the provision of bioactive compounds immunosuppressant that has been extracted from cow testes against inhibition of lymphocyte cell ploriferation. The effects of these immunosuppressant compounds can be observed from ploriferation of Mus musculus lymphocytes cells to injections (exposure) of Bali Cow Testicular Extract exposed together or without concanavalin A (Con A, as a trigger for lymphocyte cell proliferation) and Bovine Serum Albumin (BSA) as standard solution . This research was designed with Completely Randomized Design (RAL). The data obtained were analyzed by ANOVA analysis using F-test. The results of this study indicate that injection of Balinese Cervical Test Extract exposed simultaneously with BSA (ET + BSA) can increase the proliferation of Balb / c lymphocyte cell culture in In Vitro (p <0.05) compared with control and exposure of BSA and Con A together for 72 hours. However, when exposed without Con A and BSA, the Bali Cattle Testicular Extract significantly suppressed (immunosuppressed) lymphocyte cell proliferation (p <0.05) compared with exposure to Con A.
Co-Authors Baiq Asma Nufida Nofisulastri Nofisulastri