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FITOREMEDIASI MENGGUNAKAN KAYU APU (Pistia stratiotes) UNTUK MENURUNKAN KADAR COD LIMBAH CAIR TEKSTIL Utami Sari Dewi; Slamet Santoso; Elly Proklamasiningsih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4375

Waste water treatment is needed to avoid the impact of pollution to the aquatic environment. Techniques for treating waste water, among others, are through phytoremediation using the help of water lettuce (Pistia stratiotes).This research aims to determine the effect of the percent covering area of water lettuce, the length of exposure time, and interaction of both in reducing levels COD, and to determine the percent of covering area, the length of exposure time, and interaction of both that is the most efficient in reducing The result of this research showed that the percent covering area, and the length of exposure time had a very significant effect of decreasing levels of COD. The most efficient percent covering area in reducing COD was in treatment of 75%. The most efficient length of exposure time in reducing COD was in treatment 9 days. The most efficient interaction of both in reducing COD levels was at treatment of 100% coverage area with exposure time of 9 days.

Respon Penambahan Asam Humat terhadap beberapa Kandungan Senyawa Aktif Pegagan (Centella asiatica) triani hardiyati; Elly Proklamasiningsih; Iman Budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3536

The aim of this research is to know the respon of humic acid and some growth media on the contain of active biological substance in Centella asiatica (L.) Urb. Hopefully, through the application of humic acid in the combination of media, the growth and plant metabolism will improve to produce active biological substance as primar metabolism. The growth media used were : soil & sand (1 : 1) ; soil & husk (1 : 1 ) ; and soil & zeolite (1 : 2). The humic acid treatment were 4 concentration, 0 g/kg media, 4 g/kg media, 8 g/kg media and 12 g/kg media. The research method was Completely Randomized Design, with factorial as treatment design. The variable observed were alkaloid content, flavonoid content, the presence of terpenoid, and biomass weight. The research result showed that the high weight of biomass and alkaloid contain got in the addition of 8 g humic acid in media soil and sand. The high flavonoid contain got in addition of 4 g/kg media in media soil and zeolite. There was brawn ring in the extract analysis dissolved in chloroform, acetic acid, and strong sulfate acid.

Dampak Pemanasan Global terhadap Fenologi Tanaman Kecipir (Psopocarpus tetragonolobus) dan Hubungannya dengan Serangga Pollinator Yuni Rokhdita Rahayuningtyas; Eming Eming Sudiana; Elly Proklamasiningsih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3171

Global warming or often referred to as global warming is a form of imbalance of ecosystems on earth due to the process of increasing the average temperature of the atmosphere, sea, and land on earth. The impact of global warming can make agricultural plants flower faster while pollinating insects are not ready so that the reproductive cycle is disrupted. Studying temperature changes as a result of global warming on an organism can be represented by an altitude gradient. The study aims to determine the effect of temperature changes on the phenology of winged bean plants (Psophocarpus tetragonolobus) which is described by the gradient of altitude and to determine the diversity of pollinator insects. The independent variable in this study is the difference in the gradient in elevation of the place, while the dependent variable is the phenology of the development of winged bean flowers and pollinator insects. The results of the study of abiotic factors showed that air temperature and sunlight intensity decreased in line with the increase in altitude from the surface of the seawater, while the humidity increased. The phenological analysis of winged bean plants showed the influence of altitude on plant height, number of leaves and branches, time of the first appearance, number of flowers and flower size as well as the diversity of pollinator insects.

Pertumbuhan dan kandungan flavonoid bayam merah (Alternanthera amoena Voss) pada media tanam dengan pemberian asam humat dan urea Rezza Adianti; Elly Proklamasiningsih; Nurtjahjo Dwi Sasongko
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Bayam merah (Alternanthera amoena Voss) merupakan tanaman sayur yang mengandung banyak serat, vitamin, mineral serta flavonoid yang berpotensi sebagai antioksidan. Tujuan dari penelitian ini untuk mengetahui pengaruh pemberian pupuk organikasam humat dan anorganik ureaterhadap pertumbuhan dan kandungan flavonoid tanaman bayam merah dan mengetahui konsentrasiasam humat dan urea yang paling berpengaruh, serta interaksi keduanya dalam meningkatanpertumbuhan dan kandungan flavonoid tanaman bayam merah. Penelitian termasuk eksperimental denganRancangan Acak Lengkap (RAL) Faktorial yang terdiri atas dua faktor dengan 4 taraf. Faktor pertama pemberian asam humat konsentrasi 0 (kontrol); 4 g.kg-1; 8 g.kg-1; dan 12 g.kg-1. Faktor kedua berupa pemberian urea dengan taraf konsentrasi 0 (kontrol); 0,4 g.kg-1; 0,6 g.kg-1; dan0,8 g.kg-1;masing masing perlakuan diulang sebanyak tiga kali.Data dianalisis dengan ANOVA (Analysis of Variance kemudian dilanjutkan dengan uji BNT. Pemberian pupuk urea pada konsentrasi 4 g.kg-1adalah konsentrasi yang paling efektif dalam meningkatkan bobot basah dan bobot kering, namun jika urea yang dikombinasikan dengan asam humat pada semua konsentrasi yang diterapkan tidak menunjukkan adanyaperbedaan yang signifikan untuk semua parameter, yaitu bobot basah, bobot kering serta kandungan flavonoid. Kata kunci: Alternanthera amoena Voss, asam humat, flavonoid, pertumbuhan, urea

Pertumbuhan Dan Kandungan Vitamin C Tanaman Kangkung Darat (Ipomoea reptans Poir) Pada Media Zeolit Dengan Penambahan Asam Humat Adhelia Syefanis; Elly Proklamasiningsih; Iman Budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Ipomoea reptans Poir is the most favoured vegetable that mostly known by the people because in addition having good nutrition content, it is one type of vitamin C good source other than fruits. Vitamin C is an antioxidant which is required by the body to maintain the immune of body from the damaging effect of free radicals. The addition of organic compound in the form of humic acid that has a complex structure with high molecular weight and contains an active group that can increase the growth and productivity of plants. The high availability of nutrients require a carrier such as zeolite. Zeolite is a silicate mineral which have a high cation exchange capacity (CEC) (varying between 80-180 meq/100g). The addition of zeolite as a carrier is expected to increase the production of food crops such as corn. This study aimed to know the effect of humic acid with zeolite growth media for the growth and vitamin C content in Ipomoea reptans Poir and also and to determine the concentration of humic acid that most effect to the growth and vitamin C content in Ipomoea reptans Poir. The research was conducted at the Laboratory of Plant Physiology and Greenhouse, Faculty of Biology, Jenderal Soedirman University, Purwokerto by using experimental methods. The research was conducted from January 2019 to March 2019 with using completely randomized design (CRD). The parameters were observed in this study are the number of leaves, plant fresh weight, dry weight of plants, and vitamin C content. The data were analyzed by analysis of variance at the level of 95% and 99%, then following by analytical analysis BNT 95%. The results of this research showed that the addition of humic acid with a concentration of 4 g.kg-1 zeolite growing media can increase Ipomoea reptans Poir growth and the addition of humic acid to the zeolite growth media cannot increase the vitamin C content of Ipomoea reptans Poir.

Pengaruh Pemberian Asam Humat pada Media Tanam terhadap Pertumbuhan dan Kandungan Polifenol Daun Binahong (Anredera cordifolia) FNU Riyandi; Elly Proklamasiningsih; Rochmatino Rochmatino
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Tanaman Binahong (Anredera cordifolia) merupakan salah satu tanaman obat yang bermanfaat sebagai antioksidan. Kandungan senyawa aktif utama pada daun Binahong yaitu flavonoid yang merupakan salah satu senyawa golongan polifenol. Jenis flavonoid yang terkandung pada daun Binahong adalah flavonol. Penggunaan asam humat dapat meningkatkan kualitas media tanam sehingga pertumbuhan tanaman dapat meningkat. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh asam humat terhadap pertumbuhan dan kandungan polifenol daun Binahong dan menentukan konsentrasi asam humat yang efektif untuk meningkatkan pertumbuhan dan kandungan polifenol daun Binahong. Penelitian ini dilakukan di Greenhouse dan Laboratorium Fisiologi Tumbuhan Fakultas Biologi Universitas Jenderal Soedirman. Metode yang digunakan pada penelitian ini adalah metode eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari empat perlakuan asam humat pada media pasir dengan konsentrasi 0 g. kg-1; 4 g. kg-1; 8 g. kg-1; 12 g. kg-1 dan diulang sebanyak tiga kali. Parameter yang diamati pada penelitian ini adalah pertumbuhan tanaman meliputi jumlah daun, berat basah dan berat kering tanaman, serta kandungan polifenol pada daun Binahong. Data yang diperoleh dianalisis ragam atau Analisis of variance (ANOVA) dengan taraf uji 1%, kemudian dilanjutkan dengan uji Beda Nyata Terkecil (BNT) dengan taraf uji 5%. Hasil penelitian menunjukkan bahwa pemberian asam humat pada media tanam mampu meningkatkan pertumbuhan dan kandungan polifenol daun Binahong dengan konsentrasi 12 g.kg-1.

INOKULASI RIZOBAKTERIA PADA TANAMAN CABAI (Capsicum annum L.) DI TANAH SALIN Monica Widi anti; Oedjijono Oedjijono; Elly Proklamasiningsih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

A red chili plant (Capsicum spp.) is one of the horticultural commodities in Indonesia which has good economic value, so it has priority to be developed. Agriculture practices in Java island have partly switched to coastal areas which have characteristics of saline soils. Saline soils are alkaline and nutrient-poor, especially essential nutrients such as P and N. One way to restore fertility in saline soils used beneficial and fertilizing bacteria such as PGPR. Plant growth-promoting rhizobacteria (PGPR) are free-living, soil-borne bacteria, which are capable of enhancing the growth of the plant either directly or indirectly. The objectives of this research were to determain the effect of PGPR isolates to the growth of red chili plants in saline sandy soil and to determine the best PGPR isolate capable to improve the growth of red chili plant in saline sandy soil. The result showed that the inoculation of rhizobacteria significantly affected to plant height and root length of the red chili plant (Capsicum annum). The combination of 3 PGPR isolates (Azospirillum PSA 10, Azotobacter PSA 8, and Marinococcus PSA 1) was the best result in increasing the height and root length of the chili plant (Capsicum annum).

Pengaruh Asam Humat pada Media Tanam Zeolit terhadap Pertumbuhan dan Kandungan Vitamin C Sawi Hijau (Brassica juncea) Istiq Fauziah; Elly Proklamasiningsih; Iman Budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Green mustard (Brassica juncea) as one of the commonly consumed vegetables and acts as one of the main sources of C vitamin in addition to fruits. C vitamin is needed for the body because it has antioxidant. Humic acid is a component of organic acid soil which can increase the production of food crops and plantations. Zeolites have a hollow structure that allows use as a carrier of humic acid. Aside from being a career zeolite which is applied with humic acid can act as an ameliorant material, this is useful in increasing crop production. The objectives of this study are to determine the effect of humic acid with zeolite planting media on the growth and content of C vitamin on green mustard plants and determine the concentration of humic acid which most influences the growth and content of C vitamin in green mustard plants. The research was conducted at the Laboratory of Plant Physiology and Green House, Faculty of Biology, Jenderal Soedirman University, Purwokerto using an experimental method. The study was carried out from January to February 2019 using an experimental design namely Completely Randomized Design (CRD). The parameters were the number of leaves, wet weight and dry weight of roots, stems, leaves and C vitamin. The data obtained will be analyzed by analysis of variance at the confidence level of 95% and 99%, if the results are significantly different then continued with LSD at 95%. The results showed that application of humic acid in zeolite planting media had a very significant effect on growth and C vitamin content of green mustard greens. Humic acid with a concentration of 4 g.kg-1 is the most efficient concentration in increasing growth and C vitamin content of green mustard greens.

Rizofiltrasi Logam Pb (Plumbum) pada beberapa Jenis Tumbuhan Air Elly Proklamasiningsih; Hernayanti Hernayanti
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 1 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.1.189

The research about rhizofiltration of Pb in several species of aquatic plant had been conducted in a greenhouse of Biology Faculty, Jenderal Soedirman University. The aims of this research were to evaluate the aquatic plant species which has Pb accumulative characteristic and to determine Pb concentration tolerated by those plants. This research was used experimental method with split plot design. The main plot was kind of aquatic plant of Hydrilla verticillata (L.f), Pistia stratiotes L, and Salvinia molesta D.S.Mitchell. The Pb (NO3)2 concentrations of 0, 5, 10, 15 mg/l were as sub plot. Each treatment was replicated for 3 times. The parameter observed were biomass (raw weight and dry weight), the Pb absorbed whether in root and shoot, and chlorophyll content. Data collected were analyzed using F test continued by LSD. The result of this research showed that both Pistia and Salvinia planted in 10 mg/l media were able to accumulate high Pb concentration. However, Pistia were able to accumulate Pb concentration mostly in root and only part be translocated in shoot. Whereas Salvinia was able to translocate Pb concentration in shoot higher than those in of Pistia. The implications of these results for rhizofiltration are discussed.

PERTUMBUHAN DAN KANDUNGAN POLIFENOL TANAMAN KALE PADA MEDIA TANAM DENGAN PENAMBAHAN ASAM HUMAT Muhammad Faiq Fadhllurohman; Elly Proklamasiningsih; Nurtjahjo Dwi Sasongko
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 2 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.2.4750

Kale (Brassica oleracea) contains some nutritional values like: vitamin C, pro vitamin A (carotenoids), and some compound belong to polyphenol are flavonoids that benefit to its consumer for the growth and health of the body. Polyphenols are bioactive molecules which play an essential role in plants response to ultraviolet irradiation, and high temperature. Flavonoids comprise the most studied group of polyphenols. This group has a common basic structure consisting of two aromatic rings bound together by three carbon atoms that form an oxygenated heterocycle. In order to support growth and increase it’s productivity, cultivation of Kale supposed to be done in good planting media. The addition of organic subtances like humic acid in planting media has proven to increase plant growth and increase productivity in several plants. The objectives of this research were to know the influence of the influence of humic acid on the growth of plant varieties and polyphenol content and to determine the best concentration of humic acid in increasing growth and polyphenol content. The current research method was carried out experimentally a completely randomize design in a split plot method. The main plot was plant’s varieties in 2 levels: V1: Kale Nero toscana (Brassica oleracea var. palmifolia), V2: Siberian dwarf Kale (Brassica oleracea Var. Sabellica). while the sub plot was the HA concentrations in 4 levels i.e 0 g.kg-1, 4 g.kg-1, 8 g.kg-1, and 12 g.kg-1 of planting medium, The parameters used in this study are fresh and dry weight. These data were obtained by weighing the roots and shoots; root’s length, chlorophyll content, and polyphenol content in kale. The data of polyphenol content was obtained by extracting the plant material following a maceration method then calculated using the Folin ciochalteau spectrophotometric. The data of plant’s growth and polyphenol content obtained was analyzed using (F test) with standard deviation rate of 5% and 1%. The results of the analysis of variance which has a significant different, followed by the least significant difference test (LSD). And for the result is the treatment of humic acid increase the growth and polyphenol content of Kale Nero (B. oleracea var. Palmifolia) and Siberian dwarf kale (B. oleracea Var. Sabellica).

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