Garuda - Garba Rujukan Digital

p-Index From 2019 - 2024

2.374

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan Jurnal Pengolahan Hasil Perikanan Indonesia JURNAL KIMIA SAINS DAN APLIKASI Current Biochemistry Journal Indonesian Journal of Human Nutrition Journal of Mathematical and Fundamental Sciences Indonesian Journal of Pharmaceutical Science and Technology Warta IHP (Warta Industri Hasil Pertanian) Chemistry Indonesian Journal of Chemical Research Indonesian Journal of Applied Research (IJAR) Wood Research Journal : Journal of Indonesian Wood Research Society Wood Research Journal : Journal of Indonesian Wood Research Society

MEGA SAFITHRI

Departement Of Biochemistry, Faculty Of Mathematics And Natural Sciences, IPB University, Jl. Raya Dramaga IPB Campus Dramaga Bogor, West Java, 16680, Indonesia

Author-ID : 368003

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Physics

Published : 31 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4

Antibacterial Activity of Ethanol Extract of Spirulina platensis Prayoga Pannindrya; Mega Safithri; Kustiariyah Tarman
Current Biochemistry Vol. 7 No. 2 (2020)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.7.1.1

Spirulina is a microalgae that has been widely used as various supplements and medicines because of its high nutritional content. The need for new antibacterial sources to solve the problem of antibiotic resistance, makes the potential of Spirulina as an antibacterial agent necessary to be researched. This study aims to analyze the antibacterial activity of ethanol extract of Spirulina platensis obtained from the Indonesian region against Staphylococcus aureus and Escherichia coli. Spirulina samples were macerated in ethanol solvent in a ratio of 1:10 (w / v). The antibacterial test used was the disc diffusion method with clindamycin positive control. Antibacterial test results showed that S. platensis in this study did not have antibacterial activity against E. coli and S. aureus.

Molecular Docking of Active Compounds Piper crocatum on the A-Glucosidase Enzyme as Antidiabetic Mustika Weni; Mega Safithri; Djarot Sasongko Hami Seno
Indonesian Journal of Pharmaceutical Science and Technology Vol 7, No 2 (2020)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v7i2.21120

Ethanol extract of Piper crocatum leaves has inhibitory activity of α-glucosidase enzyme. Ethyl acetate fraction from Piper crocatum leaves has the highest antioxidant activity. Previous research has provided information that the ethyl acetate fraction of Piper crocatum leaves has an inhibition of α-glucosidase containing 6XO32ZSP1D, Ethyl L-serinate hydrochloride compound, Schisandrin B compound, Columbin compound, 4- (4-methoxy-phenylamino) -2 compound, 3-dihydro-1H-4a, 9-diazacyclopenta (b) fluorine-10-carbonitrile, compound 6-Amino-4- [3- (benzyloxy) phenyl] -3-tert-butyl-2,4-dihydropyrano [2, 3-c] pyrazole-5-carbonitrile, compound 4 - {{4.6-Bis [(3R, 5S) -3,5-diamino-1-piperydinyl] -1,3,5-triazine-2-yl} amino) benzenesulfonamide and compound 1.1 '- (1,4-butanediyl) bis {2,6-dimethyl-4 - [(3-methyl-1,3-benzothiazol-2 (3H) ylidene) methyl] pyridinium. This study aims to study the interaction between bioactive compounds contained in ethyl acetate fraction of Piper crocatum leaves with α-glucosidase enzyme in In Silico using AutoDock Vina, Columbin shows the lowest binding energy with binding sites with amino acids Ser240, Asp242, His280, Arg315, Glu411, Phe159, Arg442, Tyr158 and Phe303. Columbin has the stability and inhibits the α-glucosidase enzyme from S. cerevisiae better than the seven other compounds, because it has OH and CH3 groups which play a role in the interaction with around the active side of the α-glucosidase enzyme.Keywords: Columbin, In Silico, α-Glucosidase

Aktivitas Penurunan Kadar Glukosa pada Tikus yang Diinduksi Aloksan dari Ekstrak Air Angkak, Bekatul, dan Kombinasinya Hasim Hasim; Didah Nur Faridah; Mega Safithri; Husnawati Husnawati; Agus Setiyono; Hanif Alamudin Manshur
Warta Industri Hasil Pertanian Vol 37, No 2 (2020)
Publisher : Balai Besar Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32765/wartaihp.v37i2.5460

ABSTRAK: Angkak dan bekatul mengandung komponen bioaktif sebagai antihiperglikemik. Namun penelitian mengenai aktivitas antihiperglikemik angkak dan bekatul secara in vivo belum tersedia. Tujuan penelitian ini adalah menguji efektivitas pemberian ekstrak air angkak, ekstrak air bekatul serta kombinasinya dalam proses penurunan jumlah glukosa dalam darah tikus (Rattus norvegicus) strain Sprague Dawley yang diinduksi aloksan. Penelitian dilakukan dengan melakukan preparasi sampel yaitu ekstraksi air angkak dan bekatul, pengkondisian hewan uji yaitu tikus (Rattus norvegicus) strain Sprague Dawley dengan induksi aloksan, pembagian tikus menjadi sembilan kelompok. Hasil penelitian menunjukkan penurunan kadar glukosa darah tikus paling rendah adalah pada tikus kelompok V (angkak 2) dengan pemberian ekstrak air angkak 100 mg/Kg bb, dengan penurunan 61,43% lebih tinggi dibandingkan dengan kelompok III, tikus yang diberi obat glibenklamid yang turun sebesar 40,88%. Pada kelompok V, kadar glukosa darah pada hari ke-3 sebesar 256,67±174,77 mg/dL dan turun pada hari ke-12 hingga mencapai glukosa darah normal sebesar 99,00±9,64 mg/dL. Penurunan kadar glukosa darah tikus baik kelompok pemberian ekstrak bekatul maupun dengan kombinasi ekstrak air angkak dan ekstrak air bekatul tidak menunjukkan penurunan kadar glukosa darah yang signifikan.Kata kunci: aloksan, angkak, bekatul, glukosa darahABSTRACT: Bioactive compounds in red yeast rice (traditionally known as angkak) and rice bran are scientifically evidenced to exert antihyperglycemic properties. Nevertheless, in vivo studies on antihyperglycemic activity of red yeast rice and rice bran have not been reported. This current work aimed to evaluate glucose-lowering effects on alloxan-induced rats (Rattus norvegicus) strain Sprague Dawley treated with water extract of angkak, rice bran and combination of both extracts. The research was first carried out by extraction of RYR and RB, acclimatization of rats induced by alloxan, then separating them into 9 groups. The results showed that the lowest decrease in blood glucose, i.e. 61,43%, was attributed to group 5, namely rats administered with water extract of angkak (100 mg/Kg bw), being much higher compared to group 3 (rats treated with glibenclamide) reaching up to 40,88%. In terms of group 5, the level of blood glucose exhibited a gradual attenuation, from 256,67±174,77 mg/dL on day-3 to 99,00±9,64 mg/dL on day-12, which is a normal blood glucose concentration. It is noteworthy that administration of rice bran extract and combined treatment showed no significant effects on reduction of blood glucose.Keywords: alloxan, blood glucose, red yeast rice, rice bran, antihyperglycemic

Antihyperglycemic Activity of Piper crocatum Leaves and Cinnamomum burmannii Bark Mixture Extract in Streptozotocin-induced Diabetic Rats Mega Safithri Hasibuan; Sedarnawati Yasni; Maria Bintang; Anna Setiadi Ranti
Journal of Mathematical and Fundamental Sciences Vol. 48 No. 2 (2016)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2016.48.2.8

Indonesia presently has the fourth largest diabetic mellitus prevalence of all countries in the world. In a previous study, a mixture extract of Piper crocatum leaves and Cinnamomum burmannii bark showed in vitro antihyperglycemic activity. It acted as inhibitor of the a-glucosidase enzyme and had no toxic effect when it was administered orally to male and female rats for 28 days. In the present study, mixture extracts of P. crocatum leaves and C. burmannii bark were used to observe antihyperglycemic activity in diabetic rats induced with streptozotocin. Mixture extracts of P. crocatum leaves and C. burmannii bark were orally given to diabetic Sprague Dawley rats at various doses for 16 days. The results showed that the treatment led to a reduction of the blood glucose level, an increase in blood insulin level up to 170.75% at 1260 mg/kg body weight, maintaining the blood lipid level of the diabetic rats at a normal level, and an increase of pancreatic ÃŸ cells in the islets of Langerhans up to 2.2-fold at 1260 mg/kg body weight. The mixture extracts of P. crocatum and C. burmannii have antihyperglycemic activity, which enhances the number of pancreatic Î² cells.

In Silico, to Determine The Active Compounds of Black Tea and Turmeric In Increasing The Activity of The Enzyme Sod Akhmad Endang Zainal Hasan; Mega Safithri; Aziz Syamsul Huda; Rini Kurniasih
Indonesian Journal of Applied Research (IJAR) Vol. 3 No. 1 (2022): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v3i1.187

Damage to cells caused by stress can be reduced by the presence of antioxidants, one of which is Superoxide dismutase (SOD). The role of the active ingredients of black tea and turmeric will be studied using the in-silico method to identify the active compounds as components in SOD activation. The bioavailability and toxicity of the active compounds of black tea and turmeric were studied and followed by molecular docking and virtual games. The parameters studied are Gibbs's free energy (∆G) and binding site similarity (BSS). The results were analyzed using Gibbs's free energy (∆G) and binding site similarity (BSS) parameters. It was found that those that could increase the activity of Cu/Zn SOD enzymes were Epicatechin gallate (black tea) and curcumin (turmeric), with values of -9.5 and -7.4 Kcal/mol and the same BSS value of 81.8%. The control ligand used was beta amyrin. According to Lipinski's rules, Epicatechin gallate and curcumin compounds can be absorbed well and are safe for consumption. This study concludes that Epicatechin gallate, an active compound of black tea, and curcumin, an active compound of turmeric rhizome, have the best potential to increase the activity of Cu/Zn SOD enzymes based on the results of virtual screening and molecular docking. Epicatechin gallate and curcumin are predicted to be well absorbed by the body because they qualify Lipinski's rules and are not toxic and safe for consumption.

Antibacterial Effectiveness of Syzygium cumini (L.) Skeels Leaves to Escherichia coli pBR322 Janrigo Klaumegio Mere; Maria Bintang; Mega Safithri
Indonesian Journal of Chemical Research Vol 9 No 1 (2021): Edition for May 2021
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598//ijcr.2020.9-jan

Bacterial resistance is a serious problem that until now still has become a global problem. The search for new antibacterial compounds is needed to overcome this problem. Syzygium cumini (L.) Skeels is a plant known to contain bioactive compounds that can be used as an antibacterial agent. This study aims to explore the leaves of this plant as an antibacterial against Escherichia coli pBR322 resistant bacteria. Based on the antibacterial test results, extracts and leaf fractions of this plant can inhibit bacterial growth. Ethyl acetate fraction at a concentration of 800 ppm showed strong antibacterial activity with an inhibition zone diameter of 10.36Â±0.02 mm followed by ethanol extract and other fractions, namely 8.43Â±0.01 mm (ethanol extract), 7.24Â±0.01 mm (water fraction), and 1.45Â±0.01 mm (n-hexane fraction). The results of spectrophotometric MIC determination also showed inhibition against bacterial growth, which was marked by a decrease in the absorbance value at the lowest antibacterial concentration of 600 ppm from 0.454 to 0.070 at wavenumber 600 nm after 24 hours of incubation. The decrease in the absorbance value indicated that the antibacterial properties of the plant leaves at this concentration were effective in inhibiting bacterial growth.

Penambatan Molekuler Senyawa Bioaktif Daun Wungu (Graptophyllum Pictum (L) Griff) sebagai Inhibitor Tirosinase Abdul Aziz; Dimas Andrianto; Mega Safithri
Indonesian Journal of Pharmaceutical Science and Technology Vol 9, No. 2, 2022
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v9i2.36219

Melasma is a skin disorder characterized by hyperpigmentation of the skin in areas exposed to sunlight and is included in the dominant skin disorder category in several countries. Meanwhile, data from the Food and Drug Administration shows that cosmetic products have progressed from 0.01% to 57.4% in terms of registered products over the last six years. Currently, melasma treatment and cosmetic applications as a whitening agent were carried out by regulating tyrosinase activity through the use of compounds such as kojic acid which poses a health risk. Therefore, it was necessary to have other alternatives that can regulate tyrosinase activity without harming health. This study aimed to determine inhibitory effects of wungu leaf (Graptophyllum pictum (L) Griff) bioactive component as an agent to inhibit tyrosinase activity through a molecular docking approach. A total of 28 wungu leaves bioactive components as a result of the analysis of Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography-Flame Ionization Detector (GC-FID) were tested for their inhibitory effects on tyrosinase activity. Linolenic acid and phytol were ligands that have the best inhibition on tyrosinase enzyme activity with affinity energies of -5.8 kcal/mol and -5.4 kcal/mol, respectively, and inhibition constants of 55.718 M and 109.489 M.

In Silico Screening of Cinnamon (Cinnamomum burmannii) Bioactive Compounds as Acetylcholinesterase Inhibitors Zatta Yumni Ihdhar Syarafina; Mega Safithri; Maria Bintang; Rini Kurniasih
Jurnal Kimia Sains dan Aplikasi Vol 25, No 3 (2022): Volume 25 Issue 3 Year 2022
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Alzheimer’s is a progressive and neurodegenerative disease that mainly affects people aged 65 years and older. The pathophysiology of Alzheimer’s is possibly related to the depletion of the neurotransmitter acetylcholine (ACh) due to beta-amyloid plaques and neurofibrillary tangles. Secondary metabolites found in cinnamon bark (Cinnamomum burmannii) have the potential as anticholinesterases to treat Alzheimer’s symptoms. This study aimed to identify the potency of bioactive compounds from cinnamon bark as AChE inhibitors in silico through analysis of binding energy, inhibition constants, and types of interactions. The research was conducted by screening virtually 60 test ligands using the PyRx program and molecular docking using the Autodock Tools program. The results of the ligand-receptor interaction analysis showed that 12 of the 15 tested ligands had potential as AChE inhibitors. Epicatechin and medioresinol are the ligands with the best potential for AChE inhibition with affinity close to the natural ligand or donepezil. Epicatechin has a binding energy of −10.0 kcal/mol and inhibition constant of 0.0459 M, with four hydrogen bonds and seven hydrophobic bonds. Meanwhile, medioresinol has −9.9 kcal/mol binding energy and inhibition constant of 0.0543 M, with one hydrogen bond and thirteen hydrophobic bonds.

Molecular Docking of Red Betel (Piper crocatum Ruiz & Pav) Bioactive Compounds as HMG-CoA Reductase Inhibitor Bella Fatima Dora Zaelani; Mega Safithri; Dimas Andrianto
Jurnal Kimia Sains dan Aplikasi Vol 24, No 3 (2021): Volume 24 Issue 3 Year 2021
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Cholesterol plaque buildup in artery walls occurs due to oxidation of Low-Density Lipoprotein (LDL) molecules by free radicals, which are a risk factor for coronary heart disease. Piper crocatum contains active compounds that can act as HMG-CoA reductase inhibitors, such as flavonoids, alkaloids, polyphenols, tannins, and essential oils. This study aimed to predict the potential of Piper crocatum extract and fraction compounds as HMG-CoA reductase inhibitors by investigating the ligand affinity to the HMG-CoA reductase enzyme. Ligand and receptor preparation was conducted using BIOVIA Discovery Studio Visualizer v16.1.0.15350 and AutoDock Tools v.1.5.6. Molecular docking used AutoDock Vina, while ligand visualization and receptor binding used PyMOL(TM) 1.7.4.5.Edu. The receptor used was HMG-CoA reductase (PDB code: 1HWK) with atorvastatin as a control ligand. Catechin, schisandrin B, and CHEMBL216163 had the highest inhibition with affinity energies of -7.9 kcal/mol, -8.2 kcal/mol, -8.3 kcal/mol, respectively. Amino acid residues that played a role in ligand and receptor interactions were Ser684, Asp690, Lys691, Lys692.

Effect of Microencapsulation Techniques on Physical and Chemical Characteristics of Functional Beverage Based on Red Betel Leaf Extract (Piper crocatum) Mega Safithri; Susi Indariani; Rosalina Yuliani
Jurnal Kimia Sains dan Aplikasi Vol 23, No 8 (2020): Volume 23 Issue 8 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Functional drinks based on red betel leaf extract have antioxidant activity, but they still have a bitter taste. This study aims to determine the effect of microencapsulation on phenol content, antioxidant activity, and sensory quality of functional drinks based on betel leaf extract. Microencapsulation of functional drinks was made using maltodextrin coatings with concentrations of 10% and 20%. Antioxidant activity was tested by the CUPRAC method. The ready to drink (RTD) functional drink has a total phenolic content and antioxidant activity of 782.30 ± 2.54 mg GAE/g and 1660.19 ± 31.67 µmol Tr/g, respectively. These values are higher than microencapsulated functional drinks with maltodextrin (MM). The microencapsulated functional drink with 10% maltodextrin coating (MM10) is the chosen formulation since it has the smallest particle size (1.283 µm), total phenolic content of 12.90 ± 0.01 mg GAE/g and antioxidant activity of 189.41 ± 1.88 µmol Tr/g. Microencapsulated functional drinks provide sensory quality that is not significantly different (p <0.05) from ready to drink (RTD) drinks.

Co-Authors . SURYANI Abdul Aziz Agus Setiyono Akhmad Endang Zainal Hasan ANNA SETIADI RANTI Anna Setiadi Ranti Aziz Syamsul Huda Aziz Syamsul Huda Bella Fatima Dora Zaelani C Hanny Wijaya Didah Nur Faridah DIMAS ANDRIANTO Djarot Sasongko Hami Seno Dwitasari, Oczhinvia Dwitasari, Oczhinvia Fachriyan H Pasaribu Faoziduhu Bawamenewi Farah Fahma Dept. TIN Fitria Tika Pradita Hanif Alamudin Manshur Hasanah, Uswatun Hasim Hasim Hendra Susanto Hendra Susanto Husnawati Husnawati Indariani, Susi Iriani Setyaningsih Irma Rahmayani Janrigo Klaumegio Mere Kustiariyah Tarman LAKSMI AMBARSARI Mala Nurilmala Maria Bintang MARIA BINTANG Maria Bintang MARIA BINTANG Maria Bintang Maria Bintang Meydia Meydia Monita Rekasih Muhammad Assyar Mustika Weni Neni Widowati Nirmala Peni Sugiharti Oczhinvia Dwitasari Paramitha Wirdani Ningsih Marlina Pipih Suptijah Prayoga Pannindrya Puspa Julistia Puspita Rini Kurniasih Rini Kurniasih Rizsa Mustika Pertiwi Rori Theresia Rori Theresia Rosalina Yuliani SEDARNAWATI YASNI Septiyani, Dinie Susanto, Hendra Susi Indariani Syamsul Falah Takeshi Katayama Tjahja Muhandri Toshisada Suzuki Vitriyanna Mutiara Yuhendri Yundari, Yundari Zatta Yumni Ihdhar Syarafina

Title

Found 31 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 31 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 31 Documents
Search