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Single Nucleotide Polymorphism within the Promoter Region of Cynomolgus Monkey LDLR Gene Achmad - Taher; Dedy Duryadi Solihin; Sulistiyani Sulistiyani; Dewi Apri Astuti; Dondin Sajuthi
Makara Journal of Science Vol 22, No 1 (2018): March
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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Abstract

The cynomolgus monkey (Macaca fascicularis) is one of the most common nonhuman primates used as an animal model in biomedical research related to atherosclerosis. However, little is known about genetic variation in the low density lipoprotein receptor (LDLR) gene, its effect on cholesterol levels, and associated risks of atherosclerosis. Therefore this study aimed to identify genetic polymorphisms, namely single nucleotide polymorphisms (SNPs), within the promoter region of LDLR and their relationship with animal responsiveness to hypercholesterolemia due to an atherogenic diet. In this research, SNPs were studied using DNA isolated from 22 cynomolgus monkeys obtained from a previous study, consisting of two hyporesponders, 19 hyperresponders, and one extreme case. The result showed that two SNPs existed in the promoter region, namely g.−169T>C and g.−265G>A. SNP g.−265G>A showed linkages with extreme responsiveness and can be used as a potential genetic marker for extreme animals. The result of this study has extended our knowledge of polymorphism in the LDLR gene and its use in the selection of cynomolgus monkeys as animal models in research on hypercholesterolemia and atherosclerosis.
Antioksidan Beluntas Wijaya, C. Hany; Hardjosworo, Peni Suprapti; Sajuthi, Dondin
REKAPANGAN Vol 5, No 1 (2011): REKAPANGAN
Publisher : UPN VETERAN JAWA TIMUR

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Abstract

Beluntas  (Pluchea  indica  Less)  is  a  herb  that  commonly  used  as  traditional medicine  and  food.    Its  leaves  have  many  biological  activities,  because  they  contain various  phenolic  compounds.  The  phenol  concentration  of  beluntas  leaves  depends on leaf segment, earth condition, fertilizer and environmental stress.  This research is focused  to  determine   antioxidant  activity  of  three  groups  of    beluntas  leaf  segments that   includes 1-3, 4-6 and > 6.  They were ground, dried at ambient temperature for  7  days, maserated by petroleum eter (1:4 b/v) at ambient temperature for 24 hours and soxhlet  extracted  by  methanol  (1:15  b/v)  at  65oC  for  3   hours.  The  result  shows  that the  extract  contains  phytochemical   compounds  such  as  tannin,  sterol,  phenol hydroquinone  and  flavonoid.  This  extract  has  antioxidant  activity  to  scavenging  free radical  DPPH   (2,2-phenyl-1-picrylhydrazyl).  The extract of  1-3  beluntas  leaf  segment  was   more  potential  antioxidant  source  than  4-6  and  >  6  beluntas  leaf   segments,  because it had the least IC50 (inhibitor concentration) and the  highest total phenol and  total flavonoid. The IC50, total  phenol and flavonoid  total of  it were 3.71 mg/L,  234.65  mg GAE/100 g samples  and 2163.59 mg QE/ 100 g samples, respectively.
PURIFIKASI DAN PENCIRIAN ENZIM PROTEASE FIBRINOLITIK DARI EKSTRAK JAMUR MERANG Dondin Sajuthi; Irma Suparto; Yanti .; Willy Praira
Makara Journal of Science Vol 14, No 2 (2010): November
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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Abstract

Jamur merang (Volvariela volvaceae) merupakan jamur pangan yang secara empiris diduga dapat melancarkan peredaran darah sehubungan dengan kandungan enzim fibrinolitik yang dimilikinya. Penelitian ini bertujuan untuk mendapatkan enzim protease murni yang bersifat fibrinolitik dan penciriannya dari ekstrak jamur merang. Pemurnian dilakukan secara bertahap, yaitu presipitasi amonium sulfat 75%, dialisis dengan menggunakan membran dialisis (cutoff 10 kDa), dan kromatografi pertukaran ion dengan matriks DEAE Sepharose. Fraksi eluat DEAE-Sepharose aktif mempunyai dua subunit protein dengan bobot molekul 12,9 dan 15,8 kDa. Eluat memiliki aktivitas spesifik 0,383 U/mg dengan tingkat kemurniannya mencapai 2,7 kali lebih tinggi dibandingkan dengan ekstrak kasarnya. Analisis pencirian enzim dilakukan terhadap ekstrak kasar dan eluat murni. Aktivitas ekstrak kasar dan eluat mencapai optimum pada suhu 50 ºC dan pH 7 dengan waktu inkubasi 10 menit. Pada kondisi optimum, aktivitas protease eluat lebih besar daripada ekstrak kasarnya. Enzim dihambat kuat oleh inhibitor fenilmetilsulfonil fluorida dan N-p-tosil-L-lisinklorometil keton yang menunjukkan golongan protease serin. Profil zimografi fibrin memperlihatkan bahwa enzim mampu mendegradasi substrat fibrin. Hasil ini membuktikan bahwa ekstrak dan eluat protease murni dari jamur merang (V. volvaceae) mempunyai aktivitas fibrinolitik yang potensial dan dapat dimanfaatkan sebagai alternatif terapi trombolitik.
Kajian Pakan Bersumber Energi Tinggi pada Pembentukkan Monyet Obes Oktarina, Ria; Mansjoer, Sri Supraptini; Astuti, Dewi Apri; Suparto, Irma Herawati; Sajuthi, Dondin
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (72.126 KB) | DOI: 10.14203/jbi.v6i3.3145

Abstract

ABSTRACTHigh Energy Diet to Develop Obese Animal Model in Cynomolgus Monkeys (Macacafascicularis). Obesity is a primary predisposition for diseases such as metabolic syndrome(insulin resistance, blood lipid abnormality, and hypertension), type 2 diabetes, andcardiovascular disease. The effort to overcome obesity is needed by understanding thedevelopment of obesity. Therefore, it is urgent to carry out preclinical trials by using an obeseanimal model however it is still limited. The objective of this research was to study the effect ofhigh energy diet with animal fat and soluble carbohydrate to produce obese cynomolgusmonkeys (Macaca fascicularis). Animals used in this study were 15 adult males dividedequally into three treatment groups and were given diets for 4 months. The 3 groups were 1)receiving diet A consists of beef tallow without egg yolk; 2) receiving diet B consists of beeftallow and egg yolk, (energy 4,207 cal/g, fat 19.62%, and starch 60.34% in both first two diet) 3)receiving diet C consists of monkey chow as control (energy 4,330 cal/g, fat 5.55%, and starch51.38%). Measurement were taken every four weeks for body weight, crown rump length, hipdiameter, abdominal skin thickness, body mass index (BMI), nutrient consumption anddigestibility. Results showed that animals received diet B had significant increase in bodyweight and BMI at week 4 and 8. Hip diameter and abdominal skin thickness were significantstarting at week 4 in animals receiving diet with egg yolk (p<0.05) compared to the other twogroups. Protein consumption and digestibility in group with diet A and B was lower (p<0.05)compared to control animals. However, digestibility for dry matter, fat, starch and energy wereabout 90%, meaning that the rations were considered adequate in developing obese monkey.The diet formula containing tallow and egg yolk increased body weight based on BMI criteriaabove 25 kg/m2.Key words: obesity, cynomolgus, egg yolk, body mass index, digestibility
Effect of Intravenous Polyunsaturated Fatty Acids Administration on Gastric Mucosal Integrity in Pig-tailed Macaques with Obstructive Jaundice Makmun, Dadang; Sajuthi, Dondin; Daldiyono, Daldiyono; Winarto, Adi; Sulistiawati, Erni
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 12, NUMBER 1, April 2011
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.036 KB) | DOI: 10.24871/12120118-14

Abstract

Background: Acute gastric mucosal injury commonly occurs in patients with obstructive jaundice. We studied the effect of intravenous polyunsaturated fatty acids (PUFA) administration on gastric mucosal integrity in pig-tailed macaques (Macaca nemestrina) with obstructive jaundice by ligating common bile duct (CBD). Method: The study was conducted between February 2009 and May 2010 at the Primate Research Center, Bogor Agricultural Institute. Eight selected male pig-tailed macaques with 6.625 ± 0.83 kg of body weight were used and divided into two groups. In both groups, laboratory examination, including liver function tests and upper gastrointestinal endoscopy were performed before CBD ligation and every two weeks after ligation. In the first group, intravenous PUFA with the dose of 2 g/day was administered every day since four weeks post-ligation up to four weeks later, and in the second group, intravenous PUFA was administered since before ligation up to eight weeks later. Results: In both groups, increased total bilirubin, direct bilirubin, indirect bilirubin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and gamma glutamyl transpeptidase were obviously found, meanwhile decreased albumin level was detected and the cholinesterase level of both groups remained unchanged. Ulcer formation occurred among the first group during 4 weeks after CBD ligation, and these ulcers showed obvious healing within four weeks after intravenous PUFA administration. In the second group, there was no significant ulcer formation within eight weeks after CBD ligation. Conclusion: The potential appearance of acute gastric mucosal injury which reflected by ulcer formation in pig-tailed macaques with obstructive jaundice was significantly decreased by intravenous PUFA administration. We also have successfully developed animal model of obstructive jaundice by CBD ligation, based on the result of liver function tests. Keywords: acute gastric mucosal injury, intravenous PUFA administration, obstructive jaundice, pig- tailed macaques
Chemoprevention Activity of Temu Mangga Extracts Aryo Tedjo; Dondin Sajuthi; Latifah K. Darusman
Makara Journal of Health Research Vol 9, No 2 (2005): December
Publisher : Universitas Indonesia

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Abstract

The chemoprevention activity of temu mangga extracts was investigated by determination of antioxidant activity with a peroxidation number method and gluthatione-S-transferase (GST) activity in Chang medium culture and cell lysate (total GST activity). The results indicated that ethanol extract has a strong antioxidant activity. It is caused by the phenolic group in the ethanol extract. Treatment Chang cell culture with 7th and 4th ethanol fractions increased the GST activity when compared to the control. The total GST activity (cytosolic and microsomal) increased when Chang cell culture was treated with H2O2/Fe+2. The decrease of the total GST activity was observed when 7th and 4th ethanol fractions were supplemented with H2O2/Fe+2 compared to the cell culture receiving H2O2/Fe+2 only.
KAJIAN PAKAN BERSUMBER ENERGI TINGGI PADA PEMBENTUKKAN MONYET OBES Oktarina, Ria; Mansjoer, Sri Supraptini; Astuti, Dewi Apri; Suparto, Irma Herawati; Sajuthi, Dondin
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3145

Abstract

ABSTRACTHigh Energy Diet to Develop Obese Animal Model in Cynomolgus Monkeys (Macacafascicularis). Obesity is a primary predisposition for diseases such as metabolic syndrome(insulin resistance, blood lipid abnormality, and hypertension), type 2 diabetes, andcardiovascular disease. The effort to overcome obesity is needed by understanding thedevelopment of obesity. Therefore, it is urgent to carry out preclinical trials by using an obeseanimal model however it is still limited. The objective of this research was to study the effect ofhigh energy diet with animal fat and soluble carbohydrate to produce obese cynomolgusmonkeys (Macaca fascicularis). Animals used in this study were 15 adult males dividedequally into three treatment groups and were given diets for 4 months. The 3 groups were 1)receiving diet A consists of beef tallow without egg yolk; 2) receiving diet B consists of beeftallow and egg yolk, (energy 4,207 cal/g, fat 19.62%, and starch 60.34% in both first two diet) 3)receiving diet C consists of monkey chow as control (energy 4,330 cal/g, fat 5.55%, and starch51.38%). Measurement were taken every four weeks for body weight, crown rump length, hipdiameter, abdominal skin thickness, body mass index (BMI), nutrient consumption anddigestibility. Results showed that animals received diet B had significant increase in bodyweight and BMI at week 4 and 8. Hip diameter and abdominal skin thickness were significantstarting at week 4 in animals receiving diet with egg yolk (p<0.05) compared to the other twogroups. Protein consumption and digestibility in group with diet A and B was lower (p<0.05)compared to control animals. However, digestibility for dry matter, fat, starch and energy wereabout 90%, meaning that the rations were considered adequate in developing obese monkey.The diet formula containing tallow and egg yolk increased body weight based on BMI criteriaabove 25 kg/m2.Key words: obesity, cynomolgus, egg yolk, body mass index, digestibility
Peran Bone Marrow Mesenchymal Stem cells (BMMSC) dalam Perubahan Seluler Hyperplasia Kelenjar Adrenal Tikus Hipertensi Rumlaklak, Yanse Yane; Sulistiawati, Erni; Sajuthi, Dondin; Widhyari, Sus Derthi; Nugroho, Setyo Widi
JURNAL KAJIAN VETERINER PROSIDING SEMINAR NASIONAL KE-7
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v0i0.1602

Abstract

Dalam keadaan hipertensi, hyperplasia merupakan salah satu abnormalitas jaringan yang terlihat yang ditunjukkan dengan peningkatan jumlah sel dalam jaringan atau organ sehingga jaringan atau organ menjadi lebih besar ukurannya dari normal. Tujuan penelitian ini adalah untuk mengetahui efektivitas bone marrow mesenchymal stem cells (BMMSC) terhadap perubahan seluler hyperplasia pada organ kelenjar adrenal tikus hipertensi. Penelitian ini menggunakan sepuluh ekor tikus jantan strain Wistar dengan umur ± 10-12 minggu dan berat badan ± 200-250 gram, dan dibagi menjadi 2 kelompok berbeda yaitu hipertensi BMMSC (+) dan hipertensi BMMSC (-). Tikus dikondisikan hipertensi menggunakan Metode Hashinoto. Nefrektomi kanan dan ligase arteri carotid communis dilakukan pada semua tikus. Tikus disuntik dengan deoxycorticosterone acetate (DOCA), kemudian 0,12% aminopropionitrile fumarate (BAPN) ditambahkan ke dalam air minum. Larutan NaCl 1% diberikan sebagai air minum selama penelitian. Evaluasi tekanan darah hipertensi dilakukan, kemudian tikus-tikus di euthanasia untuk koleksi organ kelenjar adrenal. Organ Kelenjar adrenal di fiksasi dengan formalin 10% dan kemudian diwarnai dengan pewarnaan Periodic Acid Shiff (PAS). Data yang diperoleh kemudian dianalisis secara deskriptif kualitatif. Hasil penelitian ini membuktikan terapi hipertensi dengan menggunakan BMMSC menunjukkan perubahan hiperplasia kearah normal.
KAJIAN TINGKAH LAKU MONYET EKOR PANJANG (Macaca Fascicularis) OBES DALAM KANDANG INDIVIDU Pijoh, Deyv; Astuti, Dewi Apri; Mansjoer, Sri Supraptini; Sajuthi, Dondin; Suparto, Irma Herawati
ZOOTEC Vol 40, No 2 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35792/zot.40.2.2020.30455

Abstract

STUDY ON BEHAVIOUR OF THE LONG TAIL MONKEY (Macaca fascicularis) OBESE IN INDIVIDUAL CAGE. This study was designed to obtain information of development behaviour the obesity of long-tailed monkey (Macaca fascicularis) fed with high energy obes diet. The need of obese monkeys highly to model byomedis obesity. Obesity is now a universal problem that needs to be explored and studied. Therefore, the objective of this research was to develop obese adult male monkeys with high energy food for 12 months and monitoring their behaviour. Fifteen adult male monkeys 6-8 years old with the weight of between 4-5 kg were divided equally into three groups. The first group was body weight average 4.50 kg, the second group was body weight average 5.00 kg; and the third group was body weight average 4.75 kg. The results showed that body weight gave a significant effect (P <0.01) on daily behaviour and also gave a significant effect (P<0.01) on fed behaviour frequently,drink behaviour, agonistic behaviour, grooming behaviour, and locomotion behaviour .Keywords: Behaviour frequently, Long-tailed monkey (Macaca fascicularis), Obesity)