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All Journal Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Berkala Penelitian Hayati Jurnal Kedokteran Hewan
Suherni Susilowati
Airlangga University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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EKSPRESI SPERMATOGENESIS TIKUS PUTIH SETELAH INDUKSI DENGAN PROTEIN INSULIN LIKE GROWTH FACTOR–I COMPLEX PLASMA SEMINALIS KAMBING Suherni Susilowati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/294

Abstract

The objective of this research was to analyzed the expression of spermatogenesis on white rat after inducted by Insulin Like Growth Factor – I (IGF-I) Complex protein from goat seminal plasm. This research consisted of two phase. The fi rst phase was explorative laboratory experiment concerned with identification, isolation and spesifi cation IGF-I Complex by using gel Native Polyacrylamid gel electroforesis, electroelution and Western Blot. Result of gel Native PAGE indicated that of goat seminal plasm with Comassie Blue Stain consisted of seven bands. Result of Western Blot indicated that molecule of IGF-I Complex of goat seminal plasm to bound specific with anti IGF-I Complex at protein ribbon. The second phase concerned with expretion of spermatogenesis on white rat after induction with isolat of IGF-I Complex protein. Result of the expression spermatogenesis on white rat after induction IGF-I Complex to show the signifi cant different between three groups (p < 0.05). From result above can be concluded that IGF-I Complex protein can increase the total of spermatocyte I and spermatocyte II.
BIOAKTIVITAS INSULIN LIKE GROWTH FACTOR–I COMPLEX PLASMA SEMINALIS KAMBING TERHADAP KUALITAS SPERMATOZOA HASIL SENTRIFUGASI Suherni Susilowati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/349

Abstract

Series of exploratived laboratory experiment have been conducted to analysed the bioactifity of Insulin Like Growth Factor – I (IGF-I) Complex protein of goat seminal plasm on the membran plasm and acrosom cap of centrifugation sperm. First experiment was conducted to identificatify and isolate IGF –I Complex of goat seminal plasm by using Native - Polyacrylamide gel electroforesis. The result of analysis of Native – PAGE indicated that IGF-I Complex have molecular weight of 150 kDa. Second experiment was conducted to role of IGF-I Complex protein on the membrane plasm and acrosom cap of centrifugation sperm. Semen was collected by using artificial vagina from eight mature goat. Immediately after initial evaluation, semen was centrifugated with Bracket and Oliphant’s (BO) for 5 minutes 1800 rpm. And then divided two groups. Each group was supplemented with 3 × 106 of sperm concentration and then into group I was supplemented with BO medium and group two was supplemented with IGF-I Complex protein. The result of this experiment indicated that percentages of intact plasm membrane and intact acrosom cap were significantly (p < 0.05), between BO medium and IGF-I protein. In conclusion, IGF- I Complex protein is the optimal in improving plasm membran and acrosom cap of centrifugation sperm
Investation of Ectoparasites In Chickens In Village of Kramat District of Bangkalan Regency of Bangkalan Fania Selfiannisa; Suherni Susilowati; Poedji Hastutiek; Lucia Tri Suwanti; Kusnoto Kusnoto; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.631 KB) | DOI: 10.20473/jops.v2i2.16400

Abstract

A study on the infestation of ectoparasites in chicken’s raised in Village of Kramat, District of Bangkalan, Regency of Bangkalan was conducted between July to October 2017. A total of 60 chickens were examined based on their sex and region of the chicken’s body. Identification was carried out on ectoparasites found identified using permanent mounting methods and scraping. The types of ectoparasites were M. gallinae 77%, L. caponis 52%, M. stramineus 15%, R. sanguineus 3%, G. gallinae 2%, G. gigas 2%, and D. gallinae 2%. Data were analyzed using Chi Square and Correspondence. The results of infestation was higher in males than females, although the difference was not statistically significant (P>0.05). Results of analysis of the pattern of investation of ectoparasites in the region that includes in males and females body are M. gallinae in the region of the back. L. caponis in the region of the wings. M. stramineus. R. sanguineus in the region of the head-neck, G. gigas in the region of belly, G. gallinae in the region of the leg just found in the males body. D. gallinae in the region of the leg just found in the females body.
The effect of red dragon fruit skin extract (hylocereus polyrhizus) on the number of leydig cells, diameter of seminiferous tubules, and testicular weight of male mice (mus musculus) exposured with hot temperatures Kukuh Prastyaningtyas; Rochmah Kurnijasanti; Rahmi Sugihartuti; Suherni Susilowati; Tri Wahyu Suprayogi; Hana Eliyani
Ovozoa : Journal of Animal Reproduction Vol. 10 No. 1 (2021): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i1.2021.18-24

Abstract

This study aims to determine the effect of red dragon (Hylocereus polyrhizus) fruit peel extract (RDFPE) on the parameters of Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat (40°C). Twenty adult male mice were divided randomly into five groups. The control group (C) mice only received a placebo. Meanwhile, the treatment groups mice were exposed to heat for 45 minutes daily for 36 days and oral administration of placebo, RDFPE of 250, 500, and 1000mg/kg BW for T0, T1, T2, and T3, respectively. The result showed that heat exposure on mice (T0 group) caused a lower of all of the parameters (p <0.05) than normal mice (control group, C). RDFPE administration at a dose of 250 mg/kg BW (T1 group) and 500 mg/kg BW (T2 group) resulted in a higher value of those parameters (p <0.05) compared to the T0 group. All those parameters of the T2 group (dose of 500 mg/kg BW) were not significantly different (p >0.05) than the control group (normal mice). However, the higher dose of RDFPE (1000 mg/kg BW, T3 group) resulted in the lower values of those parameters (p <0.05) than those of the T2 group. It could be concluded that 500mg/kg BW dose of RDFPE could return Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat.
The effect of watermelon (citrullus lanatus) rind ethanolic extract on the number of leydig, sertoli, and spermatogenic cells of rat (rattus novergicus) exposed to heat Abrian Panggalih Indra Pratama; Suherni Susilowati; Lilik Maslachah; Hermin Ratnani; Tri Wahyu Suprayogi
Ovozoa : Journal of Animal Reproduction Vol. 10 No. 1 (2021): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i1.2021.7-11

Abstract

High temperatures lead to oxidative stress, which can disturb spermatogenesis process. Watermelon (Citrullus lanatus) peel contain antioxidant expected to compensate oxidative stress due to heat stress exposure. This study aimed to determine the effect of watermelon rind ethanolic extract on the number of Leydig, Sertoli, and spermatogenic cells of rats exposed to heat (40°C). Twenty rats (Rattus norvegicus) were divided randomly into five groups. In the control group (T0) rats were not exposed to heat nor given the watermelon rind extract. T1, T2, T3, and T4 groups were exposed to heat for an hour daily and orally given placebo (1% Na CMC), 100, 200, and 400 mg/kg BW of watermelon rind extract (in 1% Na CMC). Rats were treated for 52 days, and sacrificed for the testicle collection. Hematoxylin-eosin stained histological slides were prepared for the examination of Leydig, Sertoli and spermatogenic cells. The results showed no significant difference (p >0.05) in the average number of Leydig cells in rats among groups. The number of Sertoli cells and spermatogenic cells of rats exposed to heat (T1) was lower than those of the normal rats (T0 group). The dose of watermelon rind ethanolic extract at 200 mg/kg BW (T3 group) and 400 mg/kg BW (T4) increased (p <0.05) the number of Sertoli and spermatogenic cells. It could be concluded that a dose of 400mg/kg BW of watermelon rind ethanolic extract maintained the number of Leydig cells, Sertoli cells, and spermatogenic cells of rats exposed to heat.
Motilitas dan Viabilitas Spermatozoa Sapi Rambon di Desa Kemiren Banyuwangi Zeni Prastika; Suherni Susilowati; Bodhi Agustono; Erma Safitri; Ragil Angga Prastiya
Jurnal Medik Veteriner Vol. 1 No. 2 (2018): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.103 KB) | DOI: 10.20473/jmv.vol1.iss2.2018.38-42

Abstract

Sapi Rambon merupakan salah satu sapi lokal Indonesia. Tahun 2017 populasi sapi Rambon mengalami penurunan. Salah satu upaya pelestarian sapi lokal adalah melalui peningkatan populasi sapi dan inseminasi buatan. Faktor penentu keberhasilan perkawinan salah satunya adalah libido tinggi dan kualitas semen. Motilitas spermatozoa dan viabilitas spermatozoa adalah salah satu indikator fertilitas spermatozoa dan kualitas semen. Penelitain ini adalah penelitian deskriptif. Penelitian ini bertujuan untuk mengetahui motilitas dan viabilitas semen segar sapi Rambon di Desa Kemiren Kecamatan Glagah Kabupaten Banyuwangi. Semen pada penelitian ini di tampung menggunakan vagina buatan dari 6 ekor sapi Rambon jantan. Pemeriksaan motilitas spermatozoa dan viabilitas spermatozoa dilakukan di klinik Linawati Banyuwangi. Hasil pada penelitian ini disajikan dalam bentuk tabel. Parameter penelitian ini adalah motilitas dan viabilitas spermatozoa sapi Rambon. Hasil pemeriksaan motilitas spermatozoa sapi Rambon 83,3±4,1% dan viabilitas spermatozoa sapi Rambon 86,7±2,3%. Kesimpulan dari penelitian menunjukan bahwa motilitas dan viabilitas spermatozoa sapi Rambon baik dan memenuhi syarat semen beku untuk inseminasi buatan.
Pengaruh Ekstrak Kedelai (Glycine max) Terhadap Proliferasi Lapisan Endometrium Mencit (Mus musculus) Muhammad Soleh Salahuddin; Erma Safitri; Maya Nurwartanti Yunita; Suherni Susilowati; Iwan Sahrial Hamid; Aditya Yudhana
Jurnal Medik Veteriner Vol. 2 No. 1 (2019): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.497 KB) | DOI: 10.20473/jmv.vol2.iss1.2019.49-54

Abstract

Tujuan penelitian ini adalah mengetahui pengaruh ekstrak kedelai untuk meningkatan proliferasi lapisan endometrium dalam proses perkembangan organ uterus pada mencit (Mus musculus) betina. Kelompok kontrol (K) tidak diberi perlukaan. Kelompok P1 diberi ekstrak kedelai dengan dosis 12,5mg/kgBB. Kelompok P2 diberi ekstrak kedelai dengan dosis 25mg/kgBB. Kelompok P3 diberi ekstrak kedelai dengan dosis 37,5mg/kgBB. Kelompok P4 diberi ekstrak kedelai dengan dosis 50mg/kgBB. Perlakuan dilakukan setiap hari sekali selama empat belas hari. Hasil uji One Way Anova menunjukkan perbedaan yang signifikan (p<0,05) dan dilanjutkan dengan uji Duncan’s Multiple Range Test (DMRT). Ketebalan endometrium kelompok P2, P3, dan P4 berbeda secara nyata (p<0,05), dengan kelompok kontrol (K), dan kelompok kontrol (K) tidak berbeda nyata dengan kelompok P1 (p>0,05), serta kelompok P1 tidak berbeda nyata dengan kelompok P2 (p>0,05). Kandungan senyawa fitoestrogen yaitu isoflavon berupa daidzein dan genistein bekerjasama dalam mengoptimalkan proliferasi lapisan endometrium pada uterus mencit (Mus musculus) betina. Daidzein bekerja sebagai senyawa estrogenik untuk berikatan dengan reseptor estrogen pada uterus dan genistein bekerja sebagai penghambat pertumbuhan lapisan endometrium yang berlebih pada jumlah tertentu. Kesimpulan dari penelitian ini adalah ekstrak kedelai (Glycine max) efektif untuk peningkatan proliferasi lapisan endometrium pada uterus mencit betina.
Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4) Alvyan Lantang Anugrah; Hana Eliyani; Budi Utomo; Suherni Susilowati; Maslichah Mafruchati; Rochmah Kurnijasanti
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31812

Abstract

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count  in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl­4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.
Effect of fruit juices in skim milk extender in maintaining Sapudi ram spermatozoa quality at chilled temperature Ayun Tria Marga Retta; Suherni Susilowati; Sri Pantja Madyawati; Tatik Hernawati; Wurlina Wurlina; Retno Sri Wahjuni
Ovozoa : Journal of Animal Reproduction Vol. 11 No. 2 (2022): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i2.2022.49-53

Abstract

This study aimed to determine the effect of the addition of cherry tomato, california papaya, and honey melon juice into skim milk extender in maintaining the quality of Sapudi ram semen at chilled temperature (5oC) storage. Five replication of ejaculates were divided equally into four groups. In control group (T0) semen was diluted in skim milk extender, while in T1, T2, and T3 groups semen were diluted in skim milk extender contained 20% of cherry tomato, california papaya, and honey melon juice, respectively. The extended semen was stored at a chilled temperature, and semen quality (based on sperm motility, viability, and plasma membrane integrity) was observed daily for five days. The result showed that semen quality declined day by day during the five days of storage. Based on the minimum standard of post-thawed semen motility (40%), the spermatozoa of the control group only lasted by the second day. Meanwhile, in the groups with the addition of fruit juice motility could last up to the third day, with the highest motility (p <0.05) on the addition of california papaya juice. This study concluded that the addition of 20% (v/v) california papaya juice in skim milk extender could maintain the percentage of spermatozoa motility up to three days at a chilled temperature.
Comparison of different poultry egg yolks-citrate extender with green tea (Camellia sinensis) extract addition on Sapudi ram spermatozoa quality in chilled temperature storage Shinta Putri Quraini; Suherni Susilowati; Tjuk Imam Restiadi; Sri Chusniati
Ovozoa : Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.93-97

Abstract

Preservation of ram semen has an impact on the exposure of artificial conditions to the spermatozoa which was followed by overproduction of reactive oxygen species (ROS) and resulted in functional damages of spermatozoa. This study aimed to determine the effect of the combination of chicken, quail, and duck egg yolks with green tea extract (GTE) on the quality of Sapudi ram spermatozoa at 5°C storage. Ejaculates of Sapudi ram with more than 70% spermatozoa motility were divided into four groups. Semen were extended 1 : 10 in citrated-chicken egg yolk extender (control group, T0), and citrated yolk of chicken (T1), quail (T2), and duck (T3) egg added with 0.05 mg GTE per 100 mL of extender. Extended semen was kept in a tube, which was put in a beaker containing distilled water and stored in a refrigerator (5°C). Spermatozoa motility, viability, and plasma membrane integrity (PMI) were evaluated daily for five days. The results showed that GTE in duck egg yolk citrate extender could maintain the highest spermatozoa motility, viability, and PMI for five days (p <0.05). It could be concluded that the duck egg yolk citrate extender with the addition of GTE (Camellia sinensis) was beneficial for maintaining the semen of Sapudi ram spermatozoa at chilled temperatures. Further study is needed to use the extender for freezing Sapudi ram semen.
Co-Authors Abrian Panggalih Indra Pratama Aditya Yudhana Alvyan Lantang Anugrah Ayun Tria Marga Retta Bodhi Agustono Budi Utomo Erma Safitri Fania Selfiannisa Hana Eliyani Hana Eliyani Hermin Ratnani Iwan Sahrial Hamid Kukuh Prastyaningtyas Kusnoto Kusnoto Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Maslichah Mafruchati Maya Nurwartanti Yunita Muhammad Soleh Salahuddin Poedji Hastutiek Ragil Angga Prastiya Rahmi Sugihartuti Retno Sri Wahjuni Rochmah Kurnijasanti Shinta Putri Quraini Sri Chusniati Sri Pantja Madyawati Sunarso, Agus Tatik Hernawati Tjuk Imam Restiadi Tri Wahyu Suprayogi Wurlina Wurlina Zeni Prastika