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All Journal Jurnal Veteriner Aceh Journal of Animal Science Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science journal of Basic Medical Veterinary

Tyasningsih, Wiwiek

Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, PSDKU Banyuwangi, Universitas Airlangga

Author-ID : 3693458

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Terapi Fotodinamik Mempercepat Kesembuhan Luka Insisi pada Kulit Tikus Putih (Rattus novergicus) yang Diinfeksi Bakteri Methicilin-Resistant Staphylococcus aureus Yonatan Dimascahyo Budianto; Lucia Tri Suwanti; Wiwik Misaco Yuniarti; Hani Plumeriastuti; Wiwiek Tyasningsih; Boedi Setiawan
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.591 KB)

The aims of this research was to determine the effect of photodynamic therapy (PDT) on the healing of incision wounds in rat skin (Rattus norvegicus) that infected with MRSA bacteria by evaluating and give a score (0 – 4) at wound healing factors such as epithelialization, angiogenesis, inflammatory cells, fibroblast cells and collagen density. This study used 25 white rats divided randomly into 5 groups with 5 replications, namely P0 = MRSA infection wound + Povidone Iodine, P1 = MRSA infection wound + Silver Sulfadiazine, P2 = MRSA infection wound + 5-minute PDT irradiation, P3 = MRSA infection wound + 10- minute PDT irradiation and P4 = MRSA infection wound + 15-minute PDT irradiation. Histopathological observations and scoring showed epithelialization and collagen density in groups P2, P3 and P4 increased significantly compared to groups P0 and P1 (p<0.05). The histopathological observations and scoring of angiogenesis, inflammatory cells, fibroblast cells of P0 and P1 groups had a higher score than the treatment group with photodynamic therapy P2, P3 and P4 (p<0.05). This condition showed that in the P0 and P1 groups are still in the inflammatory phase, while in the P2, P3 and P4 groups have entered the remodeling phase which is characterized by high epithelialization scores and collagen density. Giving photodynamic therapy to wounds infected with MRSA can accelerate the wound healing process.

Aktivitas Antimikrob Cuka Apel terhadap Multidrug Resistance Staphylococcus aureus yang Diisolasi dari Luka Infeksi Anjing di Surabaya Elisa Herina Dimariwu; Wiwiek Tyasningsih; Jola Rahmahani; Rahaju Ernawati; Mustofa Helmi Effendi; Didik Handijatno
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.917 KB)

Staphylococcus aureus is one of the normal flora that can cause infection in injured skin. Resistance to antibiotics has an impact on the difficulty of therapeutic treatment so that other alternatives are needed. The purpose of this study was to observe the effectiveness of apple vinegar as an antimicrobial against Multidrug Resistant Staphylococcus aureus isolated from infection wounds in dogs in Surabaya. The methods in this study were the isolation of bacteria from 30 samples of dog festering wounds on Manitol Salt Agar (MSA) media and identification through macroscopic, microscopic, catalase tests, coagulase tests, hemolysis tests on Blood Agar media, and Voges–Proskauer (VP) tests. Bacteria that have included the S. aureus criteria were followed by sensitivity tests to the antibiotics Amoxycillin, Ampicillin, Gentamicin, Chloramphenichol, and Ciprofloxacin. Apple vinegar activity test was carried out using disk diffusion method against Multidrug Resistant Staphylococcus aureus. The results showed that of the seven S. aureus isolates, there were two isolates belonging to the Multidrug Resistant S. aureus. The results of the apple vinegar activity test showed the presence of antimicrobial activity shown by the formation of a clear zone around the paper disk with an average diameter of 24.06 mm at a concentration of 90%. The conclusion shows that apple vinegar has antimicrobial activity against Multidrug Resistant S. aureus which is isolated from dog festering wounds in Surabaya.

Molecular characterization of Pasteurella multocida pfhaB1 gene fragment from buffalo and cattle isolates from Nusa Tenggara Timur Indonesia Ine Karni; Didik Handijatno; Lucia Tri Suwanti; Kusnoto Kusnoto; Jola Rahmahani; Wiwiek Tyasningsih
Aceh Journal of Animal Science Vol 4, No 2: December 2019
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.4.2.13581

Almost all regions in Nusa Tenggara Timur East Nusa Tenggara (NTT) Province Indonesia are endemic areas of Haemorragic Septicaemia (HS), which is caused by Pasteurella multocida (P. multocida ) Serotypes B: 2. The fragment pfhaB1gene is one of the virulence factors of P. multocida.The objective of this study was to determaine the phylogenetic, homology of P. multocidapfhaB1gene fragment of isolatedfrom Buffalo and Cattle in NTT. The P. multocida isolateswere re-cultured and further microscopic examined the biochemical tests, PCR, sequencing, homology, and phylogenetic relatedness test. P. multocida was observed as gram negative, coccobacilus, no growth on MacConkey Agar, does not produce H2S and gas, nonmotile and indole positive, does not produce urease enzymes, does not use citrate as a carbon source, does not ferment maltose and lactose but it does ferment glucose, sucrose and mannitol. ThepfhaB1gene fragmentfrom buffalo and cattle NTT isolates and also Katha strain vaccine showed DNA band 506 bp. P.multocida isolates from buffalo and cattle in NTT have 91% - 99% score homology with the comparative isolate. The isolate P. multocida from buffalo and cattle in NTT are in one cluster and their phylogenetic relatedness is close to isolates from Iran and India. It is concluded that the pfhaB1gene fragmentof P. multocida from buffalo and cattle isolates have phylogenetic relatedness close and homolog with the other comparative isolates. Keywords: Haemorrhagic Septicaemia; Nusa Tenggara Timur; Pasteurella multocida; pfhaB1gene

Blastocystis sp. : Evaluation of polyclonal antibody prepared from crude protein for serological diagnosis using Rabbit serum Septian Hakim Susantoputro; Lucia Tri Suwanti; Rahadju Ernawati; Mufasirin Mufasirin; Setiawan Koesdarto; Wiwiek Tyasningsih; Heni Puspitasari
Aceh Journal of Animal Science Vol 5, No 2 (2020): Desember 2020
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.5.2.16780

The diagnosis of Blastocystis infection is still based on the clinical sign which is not specific and there is no available serologic test for it. This study aimed to evaluate the polyclonal antibody prepared form crude protein of Blastocystis for the development of the Blastocystis serological test. Crude protein was extracted from the yeast of Blastocystis sp, then inoculated into rabbits to produce the antibody of crude protein. The serum of rabbits would be collected before and after immunization to compare the antibody titer. The profile of crude protein was analyzed using SDS-Page. The rabbit serum was analyzed using ELISA and Western Blot. The SDS-Page result showed bands in 100 kDa, 90 kDa, 70 kDa, 60 kDa, 58 kDa, 50 kDa, 40 kDa, 35 kDa, 30 kDa and 27 kDa. The ELISA assay showed that there was an increase in antibody titer of crude protein after immunization. Western Blot showed that three proteins (30 kDa, 40 kDa and 50 kDa) having immunogenicity characteristic. It is concluded that protein 30 kDa, 40 kDa and 50 kDa prepared from the crude protein of Blastocystis sp. can be used for developing a serologic test for Blastocystis infection. Keywords: Blastocystis sp, Crude Protein, Polyclonal Antibody .

UPAYA PENINGKATAN KESEHATAN MASYARAKAT MELALUI PENGETAHUAN MENDETEKSI FORMALIN PADA DAGING AYAM DI KELURAHAN JAMBANGAN SURABAYA Jola Rahmahani; Wiwiek Tyasningsih; A. T. Soelih Estoepangestie
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 2 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

The area of Surabaya Jambangan Village consists of 29 RTs and 7 RWs. The people of Surabaya, especially the Jambangan village, prefer to consume chicken meat compared to other meats, because in addition to containing high protein nutrients as well because the price is cheap so it is affordable by the public. Today many people have circulated in the community the use of formaldehyde in chicken meat for preservation so that meat is not easily decayed, but there are still many people who do not know the dangers of health. Community Service aims to empower the community, especially mothers of PKK members through the knowledge of detecting formaldehyde in chicken meat, which focuses on the knowledge of the community in recognizing formalin and the dangers to health. Community Service Results showed that there was an increase in understanding of PKK mothers in Jambangan Surabaya Village in recognizing formalin and the dangers to health and knowing how safe, healthy, whole and halal chicken meat (ASUH).AbstrakWilayah Kelurahan Jambangan Surabaya terdiri dari 29 RT dan 7 RW. Masyarakat Surabaya khususnya kelurahan Jambangan banyak memilih mengonsumsi daging ayam dibandingkan dengan daging yang lain, karena selain mengandung gizi yang tinggi protein juga karena harganya murah sehingga terjangkau oleh masyarakat. Dewasa ini telah banyak beredar di masyarakat adanya penggunaan formalin pada daging ayam untuk pengawetan supaya daging tidak mudah busuk, namun masih banyak masyarakat yang belum mengetahui bahayanya terhadap kesehatan. Pengabdian kepada Masyarakat ini bertujuan untuk memberdayakan masyarakat khususnya ibu-ibu anggota PKK melalui pengetahuan mendeteksi formalin pada daging ayam, yang difokuskan pada pengetahuan masyarakat dalam mengenal formalin dan bahayanya terhadap kesehatan. Hasil Pengabdian kepada Masyarakat menunjukkan bahwa ada peningkatan pemahaman ibu-ibu PKK di Kelurahan Jambangan Surabaya didalam mengenal formalin dan bahayanya terhadap kesehatan serta mengenal bagaimana daging ayam yang aman, sehat, utuh dan halal (ASUH).

Prevalence and Scabies Severity Rate on a Goat at Mempawah Hilir, Mempawah District West Kalimantan Province Arum Maula Hidayah; Wiwiek Tyasningsih; Soeharsono Soeharsono; Retno Wulansari
Journal of Parasite Science (JoPS) Vol. 5 No. 1 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

This research aims to determine the prevalence and severity rate of scabies on goats in Sub-District of Mempawah Hilir, Mempawah, West Kalimantan. The goat livestock used were not determined by age, sex, and breed. The number of goats researched was 100 goats. Scabies prevalence measurement was to divide the number of scabies goats by total goats, then multiplied by 100% and measurement of the severity rate of scabies goats with scoring with grades 1-3 (low), 4-6 (moderate), and 7-9 (heavy). The results obtained scabies prevalence rate of 38% with 38 positive samples from a population of 100 goats and the percentage of severity was 42%, moderate 24%, and 34% severe. This is due to weather factors in the area and the type of cage as well as inadequate maintenance management, thus making many cases of scabies attack goats.

Deteksi Antibodi Brucella pada Sapi Perah di Kecamatan Purwoharjo Kabupaten Banyuwangi dengan Metode Rose Bengal Test (RBT) Welyanto Kurniawan Dwi; Wiwiek Tyasningsih; Ratih Novita Praja; Iwan Sahrial Hamid; Suryanie Sarudji; Muhammad Thohawi Elziyad Purnama
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Penelitian ini bertujuan mengetahui keberadaan antibodi dari bakteri brucella pada sapi perah di Kecamatan Purwoharjo Kabupaten Banyuwangi menggunakan Rose Bengal Test (RBT). Metode yang digunakan dalam penelitian ini adalah metode analisis diskriptif dengan pendekatan kuantitatif, yaitu metode yang menggambarkan keberadaan antibodi Brucella pada sapi perah dengan cepat. Informasi pendukung diperoleh dari survei peternak dengan teknik wawancara. Sampel ditentukan dengan rumus slovin dengan ukuran sampel sebanyak 75 sampel dari 294 ekor sapi pada populasi sapi perah di Kecamatan Purwoharjo. Hasil penelitian menunjukan 2 sampel dinyatakan positif Brucella dari 75 sampel. Hasil penelitian ini dapat disimpulkan bahwa reaktor Brucella ditemukan pada sapi perah di Kecamatan Purwoharjo Kabupaten Banyuwangi yang berpotensi menyebabkan penyakit Brucellosis. Harapan dari penelitian ini adalah data yang diperoleh dapat diuji ulang dan diuji lebih lanjut dalam penelitian selanjutnya sehingga hasil dapat dikonfirmasi.

Potensi Ekstrak Daun Afrika (Vernonia amygdalina Delile) Sebagai Antibakterial Terhadap Bakteri Escherichia coli ATCC 25922 Fitriani Murjianingsih; Suryanie Sarudji; Amung Logam Saputro; Wiwiek Tyasningsih; Iwan Sharial Hamid; Maya Nurwartanti Yunita
Jurnal Medik Veteriner Vol. 2 No. 1 (2019): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Tujuan dari penelitian ini adalah untuk mengetahui potensi ekstrak metanol daun Afrika (Vernonia amygdalina Delile) sebagai antibakteri terhadap bakteri Escherichia coli ATCC 25922. Isolat E. coli dibiakan pada media Muller Hinton Agar (MHA). Uji antibakteri menggunakan metode difusi sumuran. Kontrol negatif (K-) pada lubang sumuran hanya diberikan pengencer ekstrak CMC Na 0,5%. Kontrol positif (K+) pada lubang sumuran diberi dengan eugenol. Perlakuan (P1, P2, P3, P4) diberikan ekstrak metanol daun Afrika (Vernonia amygdalina Delile) dengan masing - masing konsentrasi 100%, 75%, 50% dan 25%. Rancangan penelitian menggunakan metode Rancangan Acak Lengkap (RAL), dengan enam perlakuan dan empat pengulangan. Data dianalisis dengan ANOVA, dilanjutkan dengan uji Duncan. Hasil analisis zona hambat menunjukkan, K+ memiliki perbedaan yang nyata dengan semua perlakuan K-, P1, P2, P3, dan P4. Serta K- ditemukan tidak berbeda nyata dengan perlakuan P1, P2, P3 dan P4. Hasil penelitian menunjukkan bahwa setiap perlakuan tidak menghasilkan zona hambat sehingga tidak berbeda nyata dengan kontrol negatif (CMC Na 0,5%).

Isolasi dan Identifikasi Staphylococcus aureus pada Susu Kambing Peranakan Etawah Penderita Mastitis Subklinis di Kelurahan Kalipuro, Banyuwangi Laila Nur Hayati; Wiwiek Tyasningsih; Ratih Novita Praja; Sri Chusniati; Maya Nurwartanti Yunita; Prima Ayu Wibawati
Jurnal Medik Veteriner Vol. 2 No. 2 (2019): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Staphylococcus aureus merupakan salah satu bakteri yang sering menyebabkan mastitis subklinis pada Kambing Peranakan Etawah (PE) yang menimbulkan kerugian ekonomi yang cukup besar akibat turunnya produksi susu. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi Staphylococcus aureus pada susu kambing PE pederita mastitis subklinis di Kelurahan Kalipuro, Banyuwangi. Sampel yang digunakan berupa susu kambing PE yang diambil pada bulan Desember 2018 di Kelurahan Kalipuro, Banyuwangi. Metode sampling yang digunakan yaitu metode purposive sampling dimana kriteria kandang yang menggunakan bahan baku kayu dan bambu, umur kambing PE 2-3 tahun, kambing yang aktif diperah dan terbukti positif 3 dan 4 mastitis subklinis melalui uji CMT. Sampel yag didapat sebanyak 17 kemudian dianalisis laboratorik di laboratorium mikrobiologi Fakultas Kedokteran Hewan Universitas Airlangga Banyuwangi. Tahap isolasi pada media MSA dan dilanjutkan tahap identifikasi dengan menggunakan pewarnaan Gram, uji katalase, uji manitol, uji koagulase dan uji VP. Diperoleh hasil yaitu terdapat 16 (94.1%) sampel positif Staphylococcus aureus.

Isolasi dan Identifikasi Salmonella pada Daging Sapi di Rumah Potong Hewan Banyuwangi Dwi Putri Indriyani; Wiwiek Tyasningsih; Ratih Novita Praja
Jurnal Medik Veteriner Vol. 2 No. 2 (2019): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Tujuan penelitian ini adalah untuk mengetahui keberadaan Salmonella kontaminasi pada sapi potong yang disembelih di Rumah Potong Hewan (RPH) Banyuwangi. Sampel yang digunakan dalam penelitian ini adalah daging sapi yang diambil pada Januari 2018 yang disembelih di RPH Banyuwangi. Penelitian ini mengambil 25 gram musculus paha dari daging sapi digunakan sebagai sampel dan jumlah sampel adalah 32 sampel daging sapi yang diambil dari pemotongan sapi di RPH Banyuwangi. Hasil uji isolasi dan identifikasi menggunakan IMVIC menunjukkan bahwa 3.1% sampel positif Salmonella spp. sedangkan persentase sampel negatif Salmonella spp. adalah 96.9%. Hasil penelitian menunjukkan bahwa dengan hasil sampel positif 3.1% menunjukkan adanya Salmonella spp. ini bisa jadi karena RPH Banyuwangi sebagai tempat pengambilan sampel memiliki sanitasi yang lebih baik sehingga tingkat kontaminasi yang terjadi tidak terlalu tinggi.

Co-Authors A. T. Soelih Estoepangestie Achmad Arby Wijaya Aditya Yudhana Agnes Theresia Soelih Estoepangestie Agung Jati Kusuma Amirul Muslim Amrullah Amung Logam Saputro Arum Maula Hidayah Aswin Rafif Khairullah Benjamin Chr. Tehupuring Boedi Setiawan Chusniati, Sri Dadik Raharjo Didik Handijatno Didik Handijatno Dio Putra Pratama Dwi Putri Indriyani Elisa Herina Dimariwu Emmanuel Djoko Poetranto Erma Safitri Fedik Abdul Rantam Fitriani Murjianingsih Gracia Angelina Hendarti Hana Eliyani Hani Plumeriastuti Heni Puspitasari Imbi Kusumastuti Indah Purnamasari Ine Karni Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sharial Hamid Jola Rahmahani Kusnoto Kusnoto Laila Nur Hayati Lucia Tri Suwanti, Lucia Tri Martia Rani Tacharina Maya Nurwartanti Yunita Mufasirin Muhammad Thohawi Elziyad Purnama Mustofa Helmi Effendi Nenny Harijani Prima Ayu Wibawati Rahadju Ernawati Rahaju Ernawati Rahmahani, Jola Ratih Novita Praja Ratna Ajeng Prameswari Retno Wulansari Sancaka Chasyer Ramandinianto Sarudji, Suryanie Septian Hakim Susantoputro Setiawan Koesdarto Shinta Levea Ni'matul Fadlilah Soeharsono Soeharsono Soeharsono Soeharsono Suwarno Suwarno Suwarno Suwarno Welyanto Kurniawan Dwi Wiwik Misaco Yuniarti Yonatan Dimascahyo Budianto

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