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All Journal Jurnal Veteriner Jurnal Medik Veteriner journal of Basic Medical Veterinary
Tatang Santanu Adikara
Department Of Veterinary Anatomy, Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Analisis Filogenetik Gen Hemaglutinin dan Neuraminidase Avian Influenza H9N2 Asal Ayam Petelur di Jawa Timur (PHYLOGENETIC ANALYSIS OF HAEMMAGLUTININ AND NEURAMINIDASE GENES OF AVIAN INFLUENZA H9N2 FROM LAYER INI EAST JAVA) Prestalia Dwi Rachmawati; Tatang Santanu Adikara; Hani Plumeriastuti; Rahaju Ernawati; Jola Rahmahani; Didik Handijatno; Christian Marco Hadi Nugroho
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.985 KB)

Abstract

Avian influenza virus (AIV) subtype H9N2 is one of the infectious agents that threatens laying poultry farms, because it has an impact on drastically reducing production in the population. The aim of this study was to isolate and analyze phylogenetically the partial gene encoding the surface proteins of the AIV subtype H9N2 from laying hens in East Java. A total of 30 suspected AIV subtypes of H9N2 were taken from laying hens which had decreased production by up to 70% in three sub districts each in Kediri, Blitar and Tulung Agung regency, in East Java Province. The virus was isolated in embryonated chicken eggs and then followed by a Hemagglutination (HA) test. Detection of the presence of H9 and N2 genes was carried out through Reverse Transcriptase polymerase Chain Reaction (RT-PCR) and continued with partial gene sequencing of the two surface proteins. Data analysis was processed using BioEdit version 7.2.5 and Mega 7.0. The results show that only one sample, namely code B2.2 from Blitar regency, is an AIV subtype of H9N2. The virus in this study belong to clade h9.4.2.5 of the AIV subtype H9N2. The conclusion of this study is that the VAI subtype H9N2 was successfully isolated from laying hens in East Java and successfully identified phylogenetically.
Respon Klien Terhadap Pelayanan Elektroakupunktur Hewan di Klinik La Femur Surabaya Tutik Rosita Idayati; Koesnoto Supranianondo; Sarmanu Sarmanu; Raden Tatang Santanu Adikara; Wiwik Misaco Yuniarti; Suharsono Suharsono
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.173-181

Abstract

Elektroakupunktur merupakan metode modern dalam bidang akupunktur veteriner. Penerapan pada hewan dinilai efektif, ekonomis, tidak menimbulkan efek samping sehingga klien merasa puas. Kepuasan klien berdasarkan persepsi terhadap kualitas pelayanan yaitu kesesuaian antara harapan dan kenyataan yang diterima klien. Tangibel, reliability, responsiveness, assurance, dan empathy merupakan lima unsur dimensi kualitas pelayanan. Jumlah responden yang diperoleh dalam penelitian ini yaitu sebanyak 30 responden menggunakan layanan elektroakupunktur hewan sebanyak dua kali di klinik La Femur. Data yang diperoleh kemudian dianalisis menggunakan indeks kepuasan klien dan importance performance analysis. Hasil analisis data lima dimensi kualitas pelayanan elektroakupunktur hewan klinik La Femur menunjukkan nilai tangibel 0.91%, reliability 0.97%, responsiveness 0.99%, assurance 0.99% dan empathy 0.98%. Lima dimensi pelayanan elektroakupunktur hewan klinik La Femur menunjukkan nilai diatas standart.
STIMULASI TITIK REPRODUKSI DENGAN LASERPUNKTUR SEMIKONDUKTOR TERHADAP PENAMPILAN BIRAHI SAPI BALI (Bos sondaicus) Hanifah Khairun Nisa; Budiarto Budiarto; Tatang Santanu Adikara; Herry Agoes Hermadi; Soeharsono Soeharsono; Imam Mustofa
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21088

Abstract

This research to know the stimulation of reproduction point with a semiconductor laserpuncture to estrous synchronization of Bali Cattle was conducted in Loka Penelitian Sapi Potong Grati-Pasuruan, East Java. Estrous synchronization is a technique to get the sign of estrous and ovulation (estrous outside of estrous cycle) to be easier to detect estrous, synchronization of breeding, and shorter of calving interval. Laserpuncture was applied on 8 head bali cattles at 14 reproduction acupuncture points, 10 second for each point, 3 times frequency with interval 1 day (20-24 times). The variabel of this assessment were respons and signs of estrous, synchronized of estrous with Chi square test and T-test method. Result showed that there was significant correlation between semiconductor laserpuncture with estrous synchronization (p < 0,05). The result of assessment 8 (100%) head bali cattle was estrous. The respons of estrous appeared 1 head (12,5%) on 5th day (4th day post laserpuncture), 4 head (50%) on 6th day (5th day post laserpuncture) 1 head (12,5) on 8th day (7th day post laserpuncture), and 2 head (25%) on 10th day (9th day post laserpuncture). This result showed that semi conductor laserpuncture technology was effective to stimulate estrous synchronization and resulting ovulation, with the evidence that conception was occurred.
Pengaruh Laserpunktur dan Penambahan Probiotik dalam Laju Pertumbuhan Sapi Bali (Bos sondaicus) Jantan Febriyono Anggela Prakoso; Raden Tatang Santanu Adikara; Kusnoto Kusnoto; Sri Hidanah; Yeni Dhamayanti; Sunaryo Hadi Warsito
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28586

Abstract

The purpose of this study was to determine the effect of laserpuncture shoot towards the growth rate of male Bali cattle. This reasearch was done in July until August 2018 in Cendono Village, Pasuruan. This research uses 18 samples with completely randomized design as a research methods. This research used 18 male Bali cattle aged 2 – 2.5 year. This research divided by 3 groups are the control group (P0, which is without laserpuncture shooting) and two treatment groups (P1, which is laserpuncture shooting with 0.5 joule of doses and P2, which is laserpuncture shooting with 0.5 joule of doses and bioplus probiotic). Laserpuncture shooting already done for 6 weeks with 6 days of interval. The result is weight of cuttle is increasing and it is known from digital weight scales. The data is analyzed by using ANOVA test and followed by Duncan. The result is laserpuncture shooting at the growth point can be increasing the weight of male Bali cattle. Stastical comparison amon P0, P1 and P2 showed there were significant difference on growth rate (p<0.05). The result showed that induction of laserpuncture and bioplus probiotic has effect for growth gain rate of male Bali cattle. The optimal rate of weight gain occured in the second week with laserpuncture induction at dose of 0.5 joule and the six week with laserpuncture induction at dose of 0.5 joule and bioplus probiotic.
Co-Authors Budiarto Budiarto Christian Marco Hadi Nugroho Didik Handijatno Febriyono Anggela Prakoso Hani Plumeriastuti Hanifah Khairun Nisa Herry Agoes Hermadi Imam Mustofa Koesnoto Supranianondo Kusnoto Kusnoto Prestalia Dwi Rachmawati Rahaju Ernawati Rahmahani, Jola Sarmanu Sarmanu Soeharsono Soeharsono Sri Hidanah Suharsono Suharsono Tutik Rosita Idayati Warsito, Sunaryo Hadi Wiwik Misaco Yuniarti Yeni Dhamayanti