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All Journal Jurnal Medik Veteriner journal of Basic Medical Veterinary
Budi Utomo
Departemen Reproduksi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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The Excellence of Performance, Semen Quality and Testosterone Levels on Bull in Teaching Farm, Faculty of Veterinary Medicine, Universitas Airlangga Pudji Srianto; Trilas Sardjito; Budi Utomo; Oky Setyo Widodo
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.131-136

Abstract

Study on performance, semen quality and testosterone levels of 2 young bulls (age range 3-5 years) Limousine and Simental and 2 old bulls (age range 6-8 years) types Limousin and Simental have been carried out in the Teaching Farm, Faculty of Veterinary Medicine, Universitas Airlangga. This study aimed to determine the performance, semen quality and testosterone levels can be used as a guideline whether or not the ejaculate of young and old bulls should be continued into frozen semen. Performance tests were carried out using measuring sticks and weight bands, semen quality tests included macroscopic and microscopic tests, semen concentration using a spectrophotometer and testosterone test using the Sandwich-ELISA method. The mean results of the performance test in young bulls were, while the mean for older bulls were: body weight 700 kg; hump height 136.5 cm; circumferens of chest 213 cm; body length 171 cm and scrotal circumference 35.5 cm. The performance test for old bulls is body weight 840 kg; hump height 143 cm; circumferens of chest 225.5 cm; body length 177.5 cm and scrotal circumference 40.25 cm. Mean macroscopic of semen examination for young bulls, mean volume 8 cc; milky white color; characteristic odor; The degree of acidity was 6.5 and mucous consistency, while for the old bulls the volume was 6.85; milky white color; characteristic odor; 6.5 degrees of acidity and mucous consistency. The mean microscopic examination for young bulls was mass movement ++ / +++; individual movement 80/3; concentration 745.5 million / ml; abnormalities 3% and 92.25% viability, for old bulls: movement mass ++ / +++; individual movement 77.5 / 3; concentration 1291.5 millon / ml; abnormalities 5% and viability 90%. Examination of serum testosterone levels using the Sandwich-ELISA technique was obtained by an average of 1.299 ± 0.1598 ng/ml in young bulls and 1.101 ± 0.1502 ng/ml in older bulls. This study concluded that young bulls and old bulls in the Teaching Farm, Faculty of Veterinary Medicine, Universitas Airlangga in terms of performance, semen quality and testosterone levels are suitable for processing into high quality frozen semen.
Genetic Profile of Follicle Stimulating Hormone Receptor (rFSH) in Madrasin Crossbreed Cattle Aisyah Shaumanur Artha Hidayah; Budi Utomo; Imam Mustofa
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.91-97

Abstract

The aim of this study was to know about genetic profile of Folicle Stimulating Hormone Receptor (rFSH) in Madrasin crossbreed between Madura breed and Limousin breed (Madrasin). The research are using whole blood samples from Madura breed, Limousin breed, and Madrasin crossbreed. Madura breed and Madrasin crossbreed sample were taken from Galis, Pamekasan, East Java and Limousin breed is taken from Balai Embrio Ternak (BET) Cipelang, Bogor, West Java. Samples were used for genetic profile analysis such as DNA amplification with Polymerase Chain Reaction (PCR) method and DNA sequencing in Institute of Tropical Disease, Universitas Airlangga. The result showed that DNA fragment of rFSH is 211bp. In conclusion, this study can detect rFSH gene Madrasin crossbreed which is polymorpism.
Pengaruh Probiotik Bakteri Asam Laktat dalam Air Minum Terhadap Konversi Pakan Ayam Broiler Nanda Kholifatul Azizah; Sarmanu Sarmanu; Budi Utomo; Emy Koestanti Sabdoningrum; Widya Paramitha Lokapirnasari; Koesnoto Supranianondo
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28587

Abstract

This study aimed to determine the effect of probiotics of lactic acid bacteria in drinking water on feed consumption, weight gain and feed conversion ratio of broiler chickens. This research is an experimental research. The experimental animals used in this study were 28 male broiler chickens strain Cobb 700 aged 20 days. P0 was not given the addition of probiotic lactic acid bacteria, P1 was given the addition of probiotic lactic acid bacteria Lactobacillus acidophilus in 1ml/liter drinking water, P2 was given the addition of probiotic lactic acid bacteria Lactobacillus casei in drinking water 1ml/liter, P3 was given the addition of probiotic lactic acid bacteria Lactobacillus fermentum in drinking water 1ml/liter, P4 was given the addition of probiotic lactic acid bacteria Lactobacillus plantarum in drinking water 1ml/liter, P5 was given the addition of probiotic lactic acid bacteria Pediococcus in drinking water 1ml/liter, P6 was given the addition of probiotic mix lactic acid bacteria in drinking water 1ml /liter. Collecting data for the last 1 week by weighing the remaining consumption of feed given and weight gain of broiler chickens. The data obtained were analyzed using the Analysis of Variant (ANOVA) statistical method and continued with Duncan. Based on the results of the calculation of the amount of feed consumption and weight gain in broiler chickens, it showed that the administration of probiotic lactic acid bacteria 1ml/liter of water in broiler chickens could increase body weight gain, reduce feed conversion rates but did not reduce broiler feed consumption (p<0.05).
Co-Authors Aisyah Shaumanur Artha Hidayah Emy Koestanti Sabdoningrum Imam Mustofa Koesnoto Supranianondo Nanda Kholifatul Azizah Oky Setyo Widodo Pudji Srianto Sardjito, Trilas Sarmanu Sarmanu Widya Paramitha Lokapirnasari