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APLIKASI BERBAGAI AUKSIN UNTUK MEMACU PERTUMBUHAN VEGETATIF ANGGREK DENDROBIUM PASCA AKLIMATISASI Asih, Asih; Astutik, Astutik; Sumiati, Astri
Fakultas Pertanian Vol 7, No 1 (2019)
Publisher : Universitas Tribhuwana Tunggadewi

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Abstract

Dendrobium is one of the most popular types of ornamental plants in the community. The study aims to determine the type of auxin hormones and the right concentration to stimulate the vegetative growth of Dendrobium. The study was conducted at Jalan Tlogomas green house, Lowokwaru Subdistrict, Malang. Research for 3 months (February-May 2018). Research using a completely randomized design (CRD) factorial 2 factors, namely: Factor I is the type of Auxin (H), namely: H1 = hormones NAA (Naphthalene Acetic Acid) H2 = hormones IAA (Indoleacetic Acid) and H3 = hormones IBA (Indolebutynic Acid) Factors II is Concentration (K), namely: K0 = 0 ppm (control) K1 = 50 ppm (mg / l) K2 = 100 ppm (mg / l) and K3 = 150 ppm (mg / l). Parameters of observation include growing new shoots, increasing the number of leaves, increasing the length of the leaves, increasing the width of the leaves and the percentage of life%. The results showed that there were interactions between types of auxin and concentration on the number of leaves (aged 6-12 weeks). Hormones that are more suitable to stimulate Dendrobium growth are IBA 100 ppm with an increase in the number of leaves by 1.78. IBA hormone is more suitable to stimulate Dendrobium growth characterized by the fastest growth of shoots, increase in leaf length (1.18 cm) and optimal auxin concentration is between 50-100 ppm. Dendrobium merupakan salah satu jenis tanaman hias yang paling banyak diminati oleh masyarakat. Penelitian bertujuan untuk mengetahui jenis hormon auksin dan konsentrasi yang tepat untuk memacu pertumbuhan vegetatif Dendrobium. Penelitian dilaksanakan di green house Jalan Tlogomas Kecamatan Lowokwaru, Malang. Penelitian selama 3 bulan (Februari-Mei 2018). Penelitian menggunakan Rancangan Acak Lengkap (RAL) faktorial 2 faktor yaitu: Faktor I adalah jenis Auksin (H) yaitu: H1= hormon NAA (Naphthalene Acetic Acid) H2 = hormon IAA (Indoleacetic Acid) dan H3 = hormon IBA (Indolebutynic Acid) Faktor II adalah Konsentrasi (K) yaitu: K0 = 0 ppm (kontrol) K1 = 50 ppm (mg/l) K2 = 100 ppm (mg/l) dan K3 = 150 ppm (mg/l). Parameter pengamatan meliputi saat tumbuh tunas baru, pertambahan jumlah daun, pertambahan panjang daun, pertambahan lebar daun dan presentase hidup %. Hasil penelitian menunjukan terdapat interaksi antar jenis auksin dan konsentrasi terhadap pertambahan jumlah daun (umur 6 - 12 minggu). Hormon yang lebih sesuai untuk memacu pertumbuhan Dendrobium adalah IBA 100 ppm dengan pertambahan jumlah daun sebesar 1,78. Hormon IBA lebih sesuai untuk memacu pertumbuhan Dendrobium ditandai dengan saat tumbuh tunas paling cepat, pertambahan panjang daun (1,18 cm) dan konsentrasi auksin yang optimal adalah antara 50-100 ppm.
APLIKASI AUKSIN IAA (Indole Acetic Acid) DAN PUPUK DAUN PADA PERTUMBUHAN VEGETATIF ANGGREK Phaleonopsis Setyawan, Jhon Charles David; Astutik, Astutik; Sumiati, Astri
Fakultas Pertanian Vol 7, No 1 (2019)
Publisher : Universitas Tribhuwana Tunggadewi

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Abstract

One effort to accelerate the growth of Phaleonopsis orchids can be done by giving IAA hormone and leaf fertilization. The study aimed to determine the effect of the concentration of IAA (Indole Acetic Acid) and type of leaf fertilizer on the vegetative growth of Phaleonopsis orchids. The experiment was carried out in the Green House, Tlogomas Village, Lowokwaru Subdistrict, Malang Regency from December 2016 to March 2017. The experiment was conducted using factorial Completely Randomized Design (RAL) consisting of 2 factors and 4 replications. The first factor was, IAA concentration: controls 0 ppm (I0), 100 ppm (I1), 200 ppm (I2), and 300 ppm (I3). The second factor was the type of leaf fertilizer, they were Gandasil D (P1) and Growmore (P2) with the same concentration of 2 g/L with once a week treatment. The variables observed included: when shoots appeared, leaf length, number of leaves, leaf width and percentage of living plants. Observations were made 7 times, they weer at 2, 4, 6, 8, 10, 12, and 16 weeks since the treatment. Variables observed included buds appearing, leaf length, leaf width, and life percentage. Analysis of the research data was tested using Analysis of Variance (ANOVA), if there was a real effect then it was followed by a LSD test (the Smallest Significant Difference) level of 5%. The results showed that there was no effect of IAA concentration and type of leaf fertilizer on shoots but there was an interaction between IAA concentration and type of leaf fertilizer on the percentage of Phalaenopsis orchid plants. The IAA concentration affects the increase in the length and width of the leaves, while leaf fertilizer influences the increase in the number of leaves. The best growth of Phalaenopsis orchids was obtained at 300 ppm IAA with 0.76 cm long plant growth while leaf width increase up to 16 weeks at 300 ppm IAA was not different from 200 ppm IAA with 0.47 cm leaf width increase. Growmore had an effect on the growth of Phaleonopsis orchids better than Gandasil D and it could be seen from the increase of leaves number into 3 leaves. Salah satu upaya untuk mempercepat pertumbuhan anggrek Phaleonopsis dapat dilakukan dengan pemberian hormon IAA dan pemupukan daun. Penelitian bertujuan mengetahui pengaruh konsentrasi IAA (Indole Acetic Acid) dan jenis pupuk daun terhadap pertumbuhan vegetatif anggrek Phaleonopsis. Percobaan dilaksanakan di Green House, Desa Tlogomas, Kecamatan Lowokwaru, Kabupaten Malang pada bulan Desember 2016 sampai Maret 2017. Percobaan dilakukan menggunakan Rancangan Acak Lengkap (RAL) faktorial terdiri dari 2 faktor dan 4 kali ulangan. Faktor pertama yaitu, konsentrasi IAA : kontrol 0 ppm (I0), 100 ppm (I1), 200 ppm (I2), dan 300 ppm (I3). Faktor kedua berupa jenis pupuk daun, yaitu Gandasil D (P1) dan Growmore (P2) dengan konsentrasi yang sama yaitu 2 g/L dengan pemberian 1 minggu sekali. Variabel yang diamati meliputi : saat muncul tunas, panjang daun, jumlah daun, lebar daun dan persentase tanaman hidup. Pengamatan dilakukan sebanyak 7 kali yaitu pada minggu ke-2, 4, 6, 8, 10, 12, dan 16 sejak perlakuan. Variabel yang diamati meliputi saat muncul tunas, panjang daun, lebar daun, dan persentase hidup. Analisis data hasil penelitian diuji menggunakan Analisis of Varians (Anova), apabila terdapat pengaruh nyata maka dilanjutkan dengan uji BNT (Beda Nyata Terkecil) taraf 5%. Hasil penelitian menunjukkan bahwa tidak ada pengaruh konsentrasi IAA dan jenis pupuk daun terhadap saat muncul tunas tetapi ada interaksi konsentrasi IAA dan jenis pupuk daun terhadap persentase tanaman hidup anggrek Phalaenopsis. Konsentrasi IAA berpengaruh terhadap pertambahan panjang dan lebar daun, sedangkan pupuk daun berpengaruh terhadap pertambahan jumlah daun. Pertumbuhan anggrek Phalaenopsis terbaik diperoleh pada pemberian IAA 300 ppm dengan pertambahan panjang daun 0,76 cm sedangkan pertambahan lebar daun sampai dengan umur 16 minggu pada pemberian IAA 300 ppm tidak berbeda dengan pemberian IAA 200 ppm dengan pertambahan lebar daun 0,47 cm. Growmore berpengaruh pada pertumbuhan anggrek Phaleonopsis lebih baik dibanding dengan Gandasil D yang ditunjukan oleh pertambahan jumlah daun yang lebih banyak yakni 3 helai daun.
KERAGAMAN FENOTIPE DAN PRODUKTIVITAS LABU KUNING NUSANTARA (Cucurbita moschene Dutchene) DALAM RANGKA PENGEMBANGAN VARIETAS UNGGUL Reza Prakoso Dwi Julianto; Astri Sumiati
BUANA SAINS Vol 17, No 2 (2017)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.018 KB) | DOI: 10.33366/bs.v17i2.813

Abstract

The increase of population and the depletion of productive agricultural land causes the availability of rice as the staple food as carbohidrat becomes unbalanced. Efforts to reduce dependence on rice should be made, through the diversification of alternative food crops containing high carbohydrates. Pumpkin plants (waluh) is one of the crop that have high carbohydrate content and has many types. This research aims to collect various types of yellow squash varieties as the initial material in the assembling of superior varieties, and to identify vegetative character and agronomic character of yellow squash pumpkin varieties. The research was conducted in Randuagung Village, Lumajang Regency with height of 112 asl. The materials used in are local lumajang pumpkin seeds and local poor pumpkin. The results of this study indicate that the ratio of male flowers and female flowers on both varieties of local yellow squash showed the number of male flowers more than the number of female flowers. The beta-carotene content of local lumajang is lower than the local yellow squash is seen from the color of the pumpkin the yellow is getting faded and the taste is less sweet. The resistance to the disease of both varieties of local yellow squash indicates the tolerance criteria. The male juvenile blueberry male jungle flowers are faster than the local yellow squash lumajang, but the age of the local pumpkin flower lumajang is faster than the local yellow squash.The yellow local pumpum lumajang color is relatively the same that is brown, while the varieties of local yellow pumpkin there is a difference that is brown and green.
UPAYA MENINGKATKAN PRODUKSI TANAMAN TOMAT DENGAN APLIKASI GANDASIL B Astutik Astutik; Astri Sumiati
BUANA SAINS Vol 18, No 2 (2018)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.492 KB) | DOI: 10.33366/bs.v18i2.1188

Abstract

One of efforts to increase tomato plant productions can be done by fertilization. Gandasil B was one of leaf fertilizer which can be used to fill P of plant so that it can increase the plant production. However, so far it has not been used for tomato plants. Therefore, it needs to do a research in order to find out the concentration and fertilization period of Gandasil B to tomato production. The research was conducted in Lowokwaru Village, Malang on March to August 2012. The research consisted of 8 treatment combinations, they are K0P1 (without treatment), K1P2 (1g/l per 1 week), K2P1 (2g/l per 1 week), K3P1 (3g/l per 1 week), K0P2 (without treatment), K1P2 (1g/l per 2 weeks), K2P2 (2 g/l per 2 weeks), K3P2 (3 g/l per 2 weeks). Each treatment was repeated four times.The research results can be concluded that there is concenration interaction and fertilization period to plant height at the age of 14 and 49 days after planting, leaves amount at the age of 14, 21, 28, 35, and 42 after planting, flowers amount at the age of 49 days after planting, branches amount at the age of 49 days after planting, and fruits amount. However, partially fertilization period treatment affects at flowering time, flowers amount at the age of 28.35 and 42 days after planting, and fruits weight. The best plant of Gandasil B fertilization results 25.98 fruits/plant with total weight of 1.23 kg/plant
MULTIPLIKASI MERISTEM UBIKAYU (Manihot esculenta) DALAM MEDIA MURASHIGE AND SKOOG (MS) MODIFIKASI NAA (Naphthalene Acetic Acid) DAN BA (Benzyl Adenine) Natalia Tinoncy Waro; Astutik Astutik; Astri Sumiati
BUANA SAINS Vol 20, No 2 (2020)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/bs.v20i2.2256

Abstract

This research aimed to see the effect of the addition of NAA and BA into MS media for the development of cassava micropropagation, furthermore, the composition of the media was appropriate to produce the maximum number of cassava shoots. The study was conducted using a completely randomized factorial design consisting of 2 factors, namely: Factor I: the concentration of Naphthalene Acetic Acid (NAA) consisted of 2 levels, namely: 0 mg / l (N0) and 0.1 mg / l (N1). The second factor was the concentration of Benzyl Adenine (BA) consisting of 4 levels: 1.0 mg / l (B1); 3.0 mg / l (B2); 5.0 mg / l (B3) and 7.0 mg / l (B4). Apply 8 treatment combinations, N0B1; N0B2; N0B3, N0B4, N1B1; N1B2; N1B3; N1B4. Observations were made on the variables: time of shoot initiation, number of shoots, number of leaves, and percentage of live and contaminated explants until the age of 16 weeks after sub-culture. The results showed that there was no interaction between the addition of the auxin hormone NAA and the cytokinin BA into MS medium at the time of shoot initiation, but both hormones could support the number of new shoots and the number of leaves per plantlet. Media without the addition of NAA with the addition of BA 1.0 - 3.0 mg / l was able to initiate new shoots the fastest, namely 7-10 days. The best number of shoots of cassava (4.43 shoots/explant) used MS medium without the addition of NAA with BA 3.0 mg / l for 16 weeks (4 months) of culture.
PENGGUNAAN ALAR DAN BENZYLADENIN PADA MULTIPLIKASI MERISTEM PISANG Astutik Astutik; Sutoyo Sutoyo; Astri Sumiati
BUANA SAINS Vol 21, No 2 (2021)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/bs.v21i2.3202

Abstract

Alar is a growth regulator that in certain concentrations can inhibit growth, shorten stem segments, strengthen stems to improve the quality of tissue-cultured seedlings. This study aimed to determine the effect of alar and Benzyl Adenin (BA) on banana meristem multiplication. The experiment was conducted in Tissue Culture Laboratory, Faculty of Agriculture, Tribhuwana Tunggadewi University, Malang. The study used a Completely Randomized Design consisting of two factors, namely alar concentration (A0 = 0 mg/l and A1 = 1 mg/l) and BA concentration (B1 = 1 mg/l, B2 = 3 mg/l, B3 = 5 mg/l and B4 = 7 mg/l). Observations were made for 12 weeks of culturing with the observation variables: shoot initiation, number of shoots/explants, number of leaves, and shoot height. The results showed that alar and BA did not significantly affect the development of banana meristems. The use of Alar caused the banana plantlets to be shorter and sturdy, while BA affected the number of shoots produced. BA 1 mg/l was able to increase more shoots. 
PENGARUH PEMBERIAN HORMON NAA, PUPUK GANDASIL DAN PUPUK GROWMORE PADA PERTUMBUHAN TANAMAN ANGGREK Astri Sumiati; Astutik Astutik
BUANA SAINS Vol 19, No 2 (2019)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.321 KB) | DOI: 10.33366/bs.v19i2.1744

Abstract

Orchid is an ornamental plant, including the Orchidaceae tribe, which is widely favored among the ornamental plant community because it has high economic value, unique shapes, attractive colors and longer-lasting blooming flowers, one of which is a Phalaenopsis orchid. One of the efforts to accelerate the vegetative phalaenopsis can be done with the application of the NAA hormone and leaf fertilizers such as Gandasil fertilizer and Growmore fertilizer. The aims of this research to determine the effect of NAA, Gandasil and Growmore fertilizer types on the growth of hybrid Phalaenopsis orchids. The study was conducted at Greenhouse Jalan Tlogomas Kec. Lowokwaru, Malang City, for 5 months. The experiment was carried out using a factorial Completely Randomized Design (CRD) consisting of 2 factors namely, the factor I: NAA concentration: control 0 ppm (N0), 100 ppm (N1), 200 ppm (N2), and 300 ppm (N3). Factor II: leaf fertilizer, Growmore (P1) and Gandasil D (P2). Variables observed included: when buds appeared, leaf length, leaf width and percentage of live plants. The results showed that there was an interaction between the concentration of NAA and the type of leaf fertilizer on the emergence of shoots. When the fastest shoots appear at 100 ppm NAA, Growmore fertilizer (N1P1), which is 29 days. The NAA hormone influences the increase in leaf length and leaf width until 16 weeks of age. The best growth of hybrid Phalaenopsis was obtained at the administration of 200 ppm NAA with an increase in leaf length of 1.49 cm and an increase in leaf width of 1.06 cm until 16 weeks of age. Growmore and Gandasil D did not affect the vegetative growth of hybrid Phalaenopsis Orchid plants.
ANALISIS RESIDU PESTISIDA PADA JERUK MANIS DI KECAMATAN DAU, MALANG Astri Sumiati; Reza Prakoso Dwi Julianto
BUANA SAINS Vol 17, No 1 (2017)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.783 KB) | DOI: 10.33366/bs.v17i1.574

Abstract

Citrus is one type of fruit that is highly favored by the people. Citrus plants are vulnerable to pests and plant diseases that intensive use of pesticides in the field can not be avoided. Citrus fruits commonly consumed in the form of raw materials that need to be considered food quality and safety of citrus fruits to public health. This study aims to identify and analyze pesticide residues in citrus fruits in the district of Dau, Malang (a case study in the Village Tegalwaru subdistrict Dau, Malang) as one of the centers of citrus production in East Java with the use of pesticides are quite diverse. This research is a survey and observational with cross sectional approach. Data use of pesticides by farmers was measured by a survey conducted for the farmers and merchants. The sample was 3 kg of oranges produced by three farmers and 3 kg of oranges produced by 3 merchants Village Tegalwaru District of Dau, Malang where interviews illustrate the differences in the type and frequency of the use of pesticides during the growing season oranges, which is a high level (sample A ), medium (sample B) and low (sample C). The measurement results residues acephate, carbofuran, carbosulfan, diazinon, dimethomorp, fenobucarb, profenofos, pyrethrin everything is still below the Limit of Detection (LOD) testing in the laboratory PT. Angler Biochem Lab. In profenofos residue tests on samples found to be higher farmer is 0.108 ppm compared with a sample of traders is 0,050 ppm. The conclusions of this study are bauh oranges produced by farmers in the sub-district Tegalwaru Dau, Malang get evidence that pesticide residues are still below the prescribed MRL.
ANALISA RESIDU PESTISIDA DI WILAYAH MALANG DAN PENANGGULANGANYA UNTUK KEAMANAN PANGAN BUAH JERUK Astri Sumiati; Reza Prakoso Dwi Julianto
BUANA SAINS Vol 18, No 2 (2018)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.492 KB) | DOI: 10.33366/bs.v18i2.1185

Abstract

The use of pesticides is widely used in the production of citrus fruits for protection before and after harvest. The use of pesticides on a broad scale can pollute the environment and leave residues in plants and in plants such as fruit leaves and tubers. A survey was conducted to evaluate the level of pesticide residues in citrus fruits. The survey method is carried out by recording pesticide use by farmers and traders. The survey will be conducted in Dau, Batu and Poncokusumo Districts, Malang Regency by interviewing 10 farmers and 10 orange traders in each District. The Gas Cromatography method was carried out to measure the levels of pesticide residues tested at the Angler Biochemlab Laboratory, Surabaya. Descriptive analysis was used to analyze the levels of pesticide residues in citrus fruits then compared to the Maximum Residue Limit (BMR) through SNI 7313: 2008 quality standards on agricultural products. The residue levels of profenofos in farmer samples were found to be higher at 0.108 ppm compared to the sample from traders, namely 0.050 ppm at the Tegalweru village research site. In addition, traders in the village of Poncokusumo knew the imidaclroripid content was close to the maximum threshold of 0.040 ppm in farmer samples and 0.010 ppm in the merchant sample. Decreased levels of pesticide residues on food can be done with several approaches, namely physically and chemically. Reduction of pesticide residues in agricultural products can be done in several ways including by washing products with running water for several times or can also be done by soaking in water for one hour
RESPON TANAMAN KACANG HIJAU ( Vigna radiate L. ) TERHADAP JENIS DAN DOSIS PUPUK ORGANIK Astri Sumiati; Edison Indawan; Poppy Indrihastuti; Yohanes Aryanto Dendo Ngara
BUANA SAINS Vol 21, No 1 (2021)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/bs.v21i1.2927

Abstract

The purpose of this research was to study the effectiveness of type and dose of organic fertilizer on the growth and yield of green beans. The method used in this research was a completely randomized design of a factorial pattern. The factors consisted of a type of organic fertilizer (J) (J1 = chicken manure, J2 = cow manure, J3 = bokashi municipal waste) and the dose of organic fertilizer (D) (D1 = 80 g/polybag, D2= 160 g/polybag, D3= 240 g/polybag). The results showed that there was no interaction between the type and dose of organic fertilizer on all observation parameters. Organic fertilizer type bokashi municipal waste was gave an effect on the parameters of plant height at the age of 42 DAP (53 cm), the number of leaves at the age of 42 DAP (23.23 strands). The dose of 80 g/polybag gave a significant effect on plant height parameters at 42 DAP (53 cm), the number of leaves at 42 DAP (23.22 strands).