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Penentuan pH dan Suhu Optimum Isomerisasi Pembuatan Sirup Fruktosa dari Hidrolisat Onggok Menggunakan Katalis Mg/Al Keryanti Keryanti; Ayu Ratna Permanasari; Resti Nurul Hidayah; Rizkiya Hasanah
CHEESA: Chemical Engineering Research Articles Vol 5, No 1 (2022)
Publisher : Universitas PGRI Madiun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/cheesa.v5i1.10243.1-12

Onggok merupakan limbah padat yang dihasilkan dari industri tepung tapioka yang masih mengandung karbohidrat dalam bentuk pati sebesar 70,37%, amilosa 17,22% dan amilopektin 53,15%. Onggok berpotensi untuk diolah menjadi sirup fruktosa melalui proses hidrolisis pati yang dilanjutkan dengan isomerisasi gula hidrolisat. Penelitian ini bertujuan untuk menentukan pH dan suhu optimum isomerisasi pembuatan sirup fruktosa dari hidrolisat onggok melalui eksperimen dan analisis Response Surface Methodology (RSM). Proses hidrolisis untuk memecah pati menjadi glukosa dilakukan secara enzimatis, sedangkan isomerisasi untuk mengubah glukosa menjadi fruktosa dilakukan menggunakan katalis hidrotalsit Mg/Al yang disintesis sendiri. Variasi pH dan suhu isomerisasi ditentukan menggunakan desain eksperimen RSM. Dari percobaan didapatkan bahwa kondisi optimum isomerisasi yaitu pada pH 7 dan suhu 120 oC dengan yield sebesar 36,19% dan selektivitas sebesar 25,88%. Selanjutnya, melalui contour plot dan surface plot dari analisis RSM dapat diprediksi bahwa yield dan selektivitas fruktosa dapat mencapai masing-masing sebesar 17,87% dan 35,07% pada pH 6,58 dan suhu 126,2 ⁰C.

Studi Literatur Pengaruh Pirolisis, Jenis Adsorban serta Aktivator dalam Karakterisasi Asap Cair Rony Pasonang Sihombing; Keryanti Keryanti; Fitria Yulistiani; Ayu Ratna Permanasari
Jurnal Kimia Fullerene Vol 6 No 1 (2021): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v6i2.234

Liquid smoke is a preservative solution that can be used safely. This grade 1 product can be also used as food preservative. The aim of this article is to discuss and examine the previous experimentÃ¢â‚¬â„¢s results by literature review. The outcome of this article is expected to be regenerated by other research using the existing variable which is discussed on this article or using new variable. Based on the result. There are some variables that effect the final quality of liquid smoke. Some of them are pyrolisys temperature, pyrolisys time, adsorbant type and activator type. Pyrolisys temperature used was arround 250 Ã‚Â°C Ã¢â‚¬â€œ 300 Ã‚Â°C with resulted pH 1.41 to 2.25. While for pyrolisys temperature was arround 4 Ã¢â‚¬â€œ 5 hours with phenol content arround 3.04% to 4.08%. The type of adsorbant used was zeoilte and quartz sand, which the acid total having both increment and decrement phenomenon. Activator type used were salt activator such as NaCl , NaHCO, CaCl2, Na2SO4 and base activator (NaOH) which producing the most percent acid total.

Pengaruh Variasi pH Medium terhadap Perolehan Biomassa Sel dan Laju Konsumsi Substrat Amonium pada Kultur Suspensi Sel Wortel (Daucus carota L.) Keryanti Keryanti; Ahmad Faizal; Sri Harjati Suhardi
Reka Buana : Jurnal Ilmiah Teknik Sipil dan Teknik Kimia Vol 5, No 2 (2020): EDISI SEPTEMBER 2020
Publisher : Universitas Tribhuwana Tunggadewi Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/rekabuana.v5i2.1764

ABSTRAKPada penelitian ini, sistem kultur suspensi sel wortel (Daucus carota L.) digunakan sebagai model untuk mengetahui pengaruh variasi pH terhadap pertumbuhan biomassa sel serta laju konsumsi substrat amonium dalam medium. Kalus ditumbuhkan selama 24 hari dalam sistem batch pada medium cair MS (Murashige dan Skoog) dengan penambahan 4,5 x 10-6 M kinetin dan 4,5 x 10-6 M 2,4-D serta kondisi pH awal medium berbeda yaitu pH 4,5; 5,8 dan 7,5. Perubahan pH medium diamati setiap 3 hari sekali disertai dengan pengukuran berat biomassa sel dan kandungan amonium dalam medium. Hasil pengamatan menunjukkan bahwa laju pertumbuhan spesifik biomassa sel paling tinggi terjadi pada kultur dengan kondisi pH awal medium 4,5 yaitu 0,08 hari-1 sedangkan paling rendah pada kondisi pH awal medium 5,8 (kontrol) yaitu 0,02 hari-1. Variasi pH awal medium menyebabkan perubahan dan fluktuasi nilai pH dalam medium sehingga mempengaruhi tingkat penyerapan substrat amonium dalam medium. Laju konsumsi amonium tertinggi terjadi pada kultur perlakuan pH awal medium 4,5 yaitu sebesar 0,088 mM/hari. Penggunaan amonium untuk pertumbuhan biomassa juga paling efektif pada kultur dengan kondisi pH awal medium 4,5 dengan nilai perolehan biomassa per substrat amonium mencapai 0,875 g sel/mM amonium. Penelitian ini dapat memberikan gambaran mekanisme dan efisiensi penyerapan media pada medium pertumbuhan sehingga dapat diterapkan untuk pengendalian sistem produksi terutama sistem bioreaktor. Kata kunci : amonium; batch; Daucus carota L.; pH; pertumbuhan sel ABSTRACT In this experiment, a carrot (Daucus carota L.) cell suspension system was used as a model to determine the effect of pH variations on cell biomass growth and the rate of consumption of ammonium substrates in the medium. Callus was grown for 24 days in a batch system on MS liquid medium (Murashige and Skoog) with the addition of 4.5 x 10-6 M kinetin and 4.5 x 10-6 M 2,4-D and the initial pH of the medium was different, namely pH 4,5; 5.8; and 7.5. Changes in the medium's pH were observed every three days, accompanied by cell biomass weight measurements and ammonium content in the medium. The observations showed that the highest specific cell growth rate occurred in cultures with medium pH initial conditions of 4.5, 0.08 days-1, while the lowest at medium pH conditions of 5.8 (control), 0.02 days-1. Variations in the initial pH of the medium cause changes and fluctuations in the medium's pH value so that it affects the rate of absorption of ammonium substrates in the medium. The highest ammonium consumption rate occurred in the culture medium initial pH treatment equal to 0.088 mM/day. The use of ammonium for biomass growth is most effective in cultures with an initial pH medium condition of 4.5 with the acquisition value of biomass per ammonium substrate, reaching 0.875 g cells/mM ammonium. This research can provide an overview of the mechanism and efficiency of media absorption in the growth medium to be applied to control production systems, especially in bioreactor systems.

Distilasi Azeotrop Campuran Etanol-Air untuk Meningkatkan Kadar Etanol menggunakan Entrainer Etil Asetat Retno Indarti; Rintis Manfaati; Ari Marlina; Keryanti
Fluida Vol 14 No 1 (2021): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v14i1.3426

Bioetanol yang dapat digunakan sebagai campuran bahan bakar alternatif harus memiliki kadar 99%, sehingga perlu dilakukan proses pemurnian terhadap campuran etanol yang masih mengandung air. Distilasi sederhana campuran etanol-air menggunakan bahan tambahan (entrainer) etil asetat dapat dilakukan untuk meningkatkan kadar etanol. Pada penelitian ini, pemurnian etanol dilakukan dengan proses distilasi azeotrop pada tekanan atmosfer dan suhu penangas air sebesar 70-75oC. Komposisi umpan yang terdiri dari campuran etanol yang mengandung air dan etil asetat sebagai entrainer. Penelitian dilakukan sebanyak 5 run dengan variasi penambahan etil asetat ke dalam umpan sebanyak 10% v/v, 20% v/v, 30% v/v, 40% v/v dan 50% v/v. Hasil penelitian yang diperoleh yaitu penambahan etil asetat ke dalam umpan yang menunjukkan kadar etanol tertinggi dengan waktu yang paling singkat ialah pada penambahan etil asetat sebagai entrainer yaitu sebesar 30% v/v, yang terdiri dari campuran 15 ml etil asetat dan 35 ml etanol 80%. Hasil analisis yang dilakukan dengan kromatografi gas diperoleh konsentrasi etanol sebesar 99.80% dan waktu distilasi selama 90 menit

Produksi Asam Poliglutamat Melalui Fermentasi Menggunakan Bacillus substilis Dengan Variasi Konsentrasi Asam Sitrat Citra Noer Aqsha; Deni Ramdhani; Dini Nurcahya; Ratna Yulistya Ningrum; Riski Harliyansyah; Keryanti
Fluida Vol 15 No 1 (2022): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v15i1.3520

ABSTRAK Pada penelitian ini dilakukan produksi asam poliglutamat dari asam L-glutamat dengan fermentasi menggunakan mikroorganisme Bacillus subtilis. Tujuan dari penelitian ini yaitu untuk menentukan variasi konsentrasi asam sitrat optimum dalam media fermentasi yang dapat menghasilkan asam poliglutamat dengan jumlah paling banyak. Konsentrasi asam sitrat dalam media fermentasi divariasikan pada rentang 20–50 g/L. Analisis hasil fermentasi meliputi perhitungan berat kering sel, berat asam poliglutamat, dan nilai yield. Isolasi asam poliglutamat dilakukan melalui tahapan sentrifugasi, pengendapan dengan metanol, dialisis dan liofilisasi. Hasil penelitian menunjukkan bahwa variasi terbaik dalam memproduksi asam poliglutamat dari asam L-glutamat yaitu pada konsentrasi asam sitrat 20 g/L dengan hasil asam poliglutamat sebesar 12 mg dan yield 0,6%. ABSTRACT In this study, the production of polyglutamic acid from L-glutamic acid was carried out by fermentation using the microorganism Bacillus subtilis. The purpose of this study was to find variations in the concentration of citric acid in the fermentation media that can produce high amount of polyglutamic acid. The concentration of citric acid in the fermentation medium was varied in the range of 20–50 g/L. The fermentation broth was analyzed for the calculation of cell dry weight, polyglutamic acid weight, and yield. Isolation of polyglutamic acid was carried out through centrifugation, precipitation with methanol, dialysis and lyophilization. The results showed that the best variation of citric acid in producing polyglutamic acid was 20 g/L citric acid by producing polyglutamic acid from L-glutamic acid of 0.012 g and yield of 0.6%.

Sintesis Carbon Nanodots dari Molase Menggunakan Metode Pemanasan Terbantukan Gelombang Mikro Mentik Hulupi; Nida Nabilah; Tiana Hasna Nabilah; Keryanti Keryanti; Fauzi Abdilah
Equilibrium Journal of Chemical Engineering Vol 6, No 1 (2022): Volume 6, No 1 July 2022
Publisher : Program studi Teknik Kimia UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/equilibrium.v6i1.61212

ABSTRAK. Carbon nanodots (CNDs) merupakan nanomaterial yang berukuran dibawah 10 nm. CNDs memiliki keunggulan diantaranya yaitu fotostabilitas tinggi, biokompatibilitas baik, toksisitas yang rendah, mudah dibuat, dan ramah lingkungan. Sumber karbon yang digunakan berasal dari molase karena murah dan mudah didapat serta merupakan hasil samping dari pengolahan tebu. Pada penelitian ini digunakan metode pemanasan terbantukan gelombang mikro atau Microwave Assisted Extraction (MAE) yang memberikan hasil suspensi yang homogen, pemanasan efisien, dan waktu yang singkat. Waktu iradiasi mempengaruhi energi gap CNDs dari molase yang dihasilkan. Waktu yang optimum untuk sintesis CNDs dari molase menggunakan metode MAE yaitu selama 3 menit. Efek fluoresensi telah diamati untuk suspensi CNDs yang mengubah warna kuning menjadi warna biru dibawah sinar UV 366 nm. Karakterisasi menggunakan spektrofotometri UV-Vis menunjukan serapan maksimum CNDs dari molase pada panjang gelombang 255 nm dengan energi gap sebesar 4,55 eV.Kata kunci: Carbon Nanodots, Microwave, MolaseABSTRACT. Carbon nanodots (CNDs) are nanomaterials with a size below 10 nm. CNDs have advantages such as high photostability, good biocompatibility, low toxicity, easy to manufacture, and environmentally friendly. The carbon source used comes from molasses because it is cheap and easy to obtain and is a by-product of sugarcane processing. In this study, the Microwave Assisted Extraction (MAE) method was used which gave homogeneous suspension results, efficient heating, and short time. The irradiation time affects the energy gap of the CNDs of the molasses produced. The optimum time for the synthesis of CNDs from molasses using the MAE method is 3 minutes. Fluorescence effects have been observed for suspensions of CNDs which turn yellow to blue under 366 nm UV light. Characterization using UV-Vis spectrophotometry showed the maximum absorption of CNDs from molasses at a wavelength of 255 nm with an energy gap of 4.55 eV.Keywords: Carbon Nanodots, Microwave, Molasses

Sintesis Zn-BDC dengan Metode Sonokimia dan Aplikasinya Pada Proses Adsorpsi Ion Logam Pb2+ Fauzi Abdilah; Mentik Hulupi; Keryanti Keryanti; Nida Nabilah; Tiana Hasna Nabilah
REACTOR: Journal of Research on Chemistry and Engineering Vol 3, No 1 (2022): Published in June 2022
Publisher : Politeknik ATI Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52759/reactor.v3i1.48

The heavy metal ion pollution such as lead (Pb2+) in wastewater is an environmental problem that needs to be solved. The adsorption method has been reported to have good potential as an alternative method for reducing heavy metal contents in aquatic environment. The most widely used adsorbent media are porous materials with a large surface area and low density. Metal Organic Frameworks (MOFs) are a type of porous material that is widely applied in various fields, such as fuel purification, solvent recovery, gas storage, and adsorbents. Lead(II) is a heavy metal ion that can pollute the environment and endanger humans. This study aims to synthesize MOFs and apply it to the Pb2+ adsorption process. In this research, MOFs type Zn-BDC or MOF-5 were synthesized by sonochemistry method at 60 ℃ as adsorbent for Pb2+ ion. Characterization of the adsorbent by FTIR showed the presence of functional groups C=O, C-H, and Zn-O which indicated the formation of the MOF-5 compound. The concentrations of Pb2+ ions were analyzed using Atomic Absorption Spectrophotometer (AAS). The experimental results show the optimum conditions for the adsorption process at pH 5 for 60 minutes with adsorption efficiency reaching 93.41%. Testing the adsorption isotherm model showed that the Pb2+ ion adsorption process using Zn-BDC as an adsorbent followed the Langmuir isotherm model with the R2 value of 0.9986.

Fermentasi Kopi Arabika Menggunakan Fermentor Skala UMKM Tri Hariyadi; Zahra Nur Salsabila; Gianistri Maulani; Keryanti Keryanti
Fluida Vol 15 No 2 (2022): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v15i2.4391

Coffee farmer in Babakan Kertasari Village requires a fermenter that can ferment 100 kg of coffee cherries per day to fulfill the market demand. Therefore, the object of this study is to make a coffee fermenter with SMEs scale and to evaluate the performance of the fermenter with the addition of 130 ml/L of Local Microorganism (MOL) made from fermented cassava (cassava tape). Fermentation was carried out for 72 hours in room temperature and in semi-aerobic condition with 14 kg of coffee cherries in SMEs scale fermenter. Evaluation of the fermenter performance was done by analysing pH and ethanol content and organoleptic test of taste and aroma of fermented coffee. The result of analysis shows that fermented Arabica coffee which called cultured coffee fulfilled the National Indonesian Standard (SNI) with pH greater than 4 and caffeine content of 1.97%. Organoleptic test showed that 56% of panellists really like the aroma and 24% of panellists really like the taste of cultured coffee.

Produksi Asam Poliglutamat Melalui Fermentasi Menggunakan Bacillus substilis Dengan Variasi Konsentrasi Asam Sitrat Citra Noer Aqsha; Deni Ramdhani; Dini Nurcahya; Ratna Yulistya Ningrum; Riski Harliyansyah; Keryanti Keryanti; Ade Safaat
Fluida Vol 15 No 1 (2022): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v15i1.3520

ABSTRAK Pada penelitian ini dilakukan produksi asam poliglutamat dari asam L-glutamat dengan fermentasi menggunakan mikroorganisme Bacillus subtilis. Tujuan dari penelitian ini yaitu untuk menentukan variasi konsentrasi asam sitrat optimum dalam media fermentasi yang dapat menghasilkan asam poliglutamat dengan jumlah paling banyak. Konsentrasi asam sitrat dalam media fermentasi divariasikan pada rentang 20–50 g/L. Analisis hasil fermentasi meliputi perhitungan berat kering sel, berat asam poliglutamat, dan nilai yield. Isolasi asam poliglutamat dilakukan melalui tahapan sentrifugasi, pengendapan dengan metanol, dialisis dan liofilisasi. Hasil penelitian menunjukkan bahwa variasi terbaik dalam memproduksi asam poliglutamat dari asam L-glutamat yaitu pada konsentrasi asam sitrat 20 g/L dengan hasil asam poliglutamat sebesar 12 mg dan yield 0,6%. ABSTRACT In this study, the production of polyglutamic acid from L-glutamic acid was carried out by fermentation using the microorganism Bacillus subtilis. The purpose of this study was to find variations in the concentration of citric acid in the fermentation media that can produce high amount of polyglutamic acid. The concentration of citric acid in the fermentation medium was varied in the range of 20–50 g/L. The fermentation broth was analyzed for the calculation of cell dry weight, polyglutamic acid weight, and yield. Isolation of polyglutamic acid was carried out through centrifugation, precipitation with methanol, dialysis and lyophilization. The results showed that the best variation of citric acid in producing polyglutamic acid was 20 g/L citric acid by producing polyglutamic acid from L-glutamic acid of 0.012 g and yield of 0.6%.

Hydrolysis of Waste Cooking Oil Using Rhizopus oryzae to Produce Free Fatty Acids Rintis Manfaati; Prans Connery Manurung; Muhamad Nur Rojab; Keryanti Keryanti
Fluida Vol 16 No 1 (2023): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v16i1.4496

Waste cooking oil is waste produced from palm oil after it has been heated and fried at high temperatures, and it can pollute the environment. One effort to reuse waste cooking oil is a fermentation processe that produces free fatty acids with the help of Rhizopus oryzae as a biocatalyst. Variations in initial substrate concentration ranged from 10 g/L to 70 g/L, followed by varied types of nitrogen sources, namely malt extract, beef extract, (NH2)2CO, NH4Cl, and (NH4)2SO4 at a concentration of 70 g/L to determine free fatty acid concentration. Fermentation was carried out for 7 days. The analysis carried out included the concentration of free fatty acids, biomass, and YP/X value. The optimum initial used cooking oil substrate was obtained at a concentration of 30 g/L with a YP/X value of 13.63%, a free fatty acid concentration of 2.13 g/L and a dry cell weight of 15.48 g/L. The best nitrogen source is beef extract with a Yp/x value of 11.78%, a free fatty acid concentration of 2.02 g/L and a dry cell weight of 17.0 g/L

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