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Sari Haryanti
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Ethanolic Extract of Hedyotis corymbosa L. Increases Cytotoxic Activity of Doxorubicin on MCF-7 Breast Cancer Cell Sari Haryanti; Sendy Junedi; Edy Meiyanto
Indonesian Journal of Biotechnology Vol 14, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.106 KB) | DOI: 10.22146/ijbiotech.7809

Abstract

Hedyotis corymbosa L. with ursolic acid as the main compound is one of the plants that has been used for traditional medicine including to cure breast cancer disease. The aim of this research is to examine the cytotoxic activity of rumput mutiara herb ethanolic extract (ERM) and its effect in combination with doxorubicin against MCF-7 breast cancer cell line as cell model of doxorubicin resistance. Hedyotis corymbosa L. herb powder extraction was done by maceration using ethanol 96% then the extract is detected for ursolic acid content. Cell viability assay of ERM, doxorubicin and  the combination of ERM and doxorubicin treatments were carried out by MTT assay to determine IC50 and CI (Combination Index). Cell cycle distribution was determined by flowcytometry. Apoptosis assay was performed by ethidum bromide-acridine orange DNA staining method. Investigation on Bcl-2 expression was determined by immunocytochemistry method. Thin Layer Chromatography of ERM had similar Rf with ursolic acid standard: 0,6. ERM and doxorubicin inhibited cell growth against MCF-7 with IC50  of 77 µg/mL and 349 nM (0,19 µg/mL) respectively. Combination of ERM and doxorubicin showed synergistic effect (CI 0.66-0.99). Combination of 25 ìg/mL ERM- 200 nM doxorubicin induced apoptosis and decreased Bcl-2 expression but showed no cell accumulation on cell cycle. Doxorubicin induced high cell accumulation in G2/M phase, but ERM at the concentration of 25 ìg/mL had a low effect in G1 phase, and ERM IC50 did not induce cell accumulation otherwise apoptosis. These results concluded that the apoptosis mechanism of combination doxorubicin-ERM is mediated by cell cycle arrest and non cell cycle arrest. Therefore ERM has a potential activity to be developed as co-chemotherapeutic agent.  
Karakterisasi Morfologi dan Kandungan Minyak Atsiri Beberapa Jenis Sirih (Piper sp.) Yuli Widiyastuti; Sari Haryanti; Dyah Subositi
Proceeding of Mulawarman Pharmaceuticals Conferences Vol. 3 (2016): Spesial Issue of Mulawarman Pharmaceuticals Conference Proceeding (Prosiding Semnas T
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.53 KB) | DOI: 10.25026/mpc.v3i2.148

Abstract

Piper merupakan salah satu marga dalam famili Piperaceae yang meliputi lebih dari seribu jenis tumbuhan yang tersebar di daerah tropis dan sub tropis. Sirih (Piper betle L.) adalah salah satu spesies dalam genus Piper yang sangat dikenal masyarakat, karena tidak hanya dimanfaatkan sebagai herbal namun juga memiliki nilai penting dalam kultur atau budaya masyarakat. Berdasarkan bentuk dan warna daun, rasa dan aroma, dikenal beberapa jenis sirih antara lain sirih cacing, sirih Jawa, sirih gading, sirih banda, dan sirih cengkih. Untuk itu dilakukan penelitian karakterisasi morfologi dan kandungan minyak atsiri beberapa jenis sirih yang berasal dari beberapa daerah. Penelitian dilakukan secara eksploratif dengan mengambil spesimen beberapa jenis sirih, kemudian dilakukan karakterisasi morfologi di Laboratorium Sistematika Tumbuhan. Analisis minyak atsiri dilakukan secara stahl destilasi dan identifikasi kandungan minyak atsiri secara Kromatografi Lapis Tipis menggunakan fase diam Silikagel G dan fase bergerak toluene:etyl acetat: 93:7, visualisasi menggunakan vanillin asam sulfat. Hasil penelitian menunjukkan bahwa terdapat beberapa jenis sirih yang dikenal dengan nama daerah sirih gading, sirih cacing, dan sirih hijau dengan nama botani Piper betle L., serta sirih merah dengan nama botani Piper crocatum L. atau Piper ornatum L. Perbedaan morfologi yang paling menonjol ada pada bentuk daun, tekstur daun, warna batang, warna tangkai daun, habitus, dan aroma. Kadar minyak atsiri masing-masing jenis sirih berturut turut: sirih hijau (0,6 %), sirih cacing (0,3%), sirih gading (0,3%), dan sirih merah (0,6%). Profil KLT dari minyak atsiri masing-masing jenis sirih menunjukkan perbedaan dalam jumlah spot maupun warna spot.
Co-Authors Dyah Subositi Edy Meiyanto Sendy Junedi Yuli Widiyastuti