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Optimum Fermentation Process for Red Macroalgae Gelidium latifolium and Gracillaria verrucosa Kawaroe, Mujizat; Sari, Dahlia Wulan; Hwangbo, Junkwon; Santoso, Joko
Journal of Engineering and Technological Sciences Vol 47, No 6 (2015)
Publisher : ITB Journal Publisher, LPPM ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.11 KB) | DOI: 10.5614/j.eng.technol.sci.2015.47.6.7

Abstract

Red macroalgae have the potential to be processed into bioethanol due to their high carbohydrate and low lignin content. Gelidium latifolium and Gracilaria verrucosa are red macroalgae commonly found in Indonesian seas. Sometimes an over-supply of red macroalgae is rejected by the food industry, which opens up opportunities for others uses, e.g. for producing bioethanol. The objectives of this research were to analyze the influence of sulfuric acid concentration on hydrolysis of G. latifolium and G. verrucosa and to calculate the optimum fermentation process to produce bioethanol. G. latifolium and G. verrucosa were hydrolyzed using H2SO4 at concentrations of 1%, 2%, 3%, and 4%, at a temperature of 121 °C and a pressure of 1.5 bar for 45 minutes. The process of fermentation was done using Saccharomyces cerevisiae in anaerobic conditions for 4, 5, 6 and 7 days. The results show that the optimum H2SO4 concentrations to hydrolyze G. latifolium and G. verrucosa were 1% and 2% respectively. The number of S. cerevisiae cells in hydrolysate G. latifolium and G. verrucosa increased in the third adaptation. S. cerevisiae can convert sugar from G. latifolium and G. verrucosa into bioethanol through fermentation. The highest bioethanol yields were achieved on days five and six. Therefore, red macroalgae can be seen as a potential raw material for bioethanol production.
Perubahan Luas Penutupan Padang Lamun Di Kepulauan Seribu, DKI Jakarta Kawaroe, Mujizat; Jaya, Indra; S., Indarto H.; Sari, Dahlia Wulan; W., Sundari Wening
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (732.56 KB) | DOI: 10.24002/biota.v15i1.2641

Abstract

Seagrass is one of productive and economical marine ecosystem, but recently there are indications that this area is decreasing and every kind of damage is happening. The research was conducted to reveal the change of seagrass width area and to determine the suitable location for donor and acceptor of seagrass transplantation. Methods of research were collecting and analyzing the satellite image of the research location, bathymetri mapping and water quality parameter, and also determining environment charasteristics that suitable to be performed by counting Seagrass Transplantation Suitability Index (STSI). The results show that in year 1999-2004, the derivation area of seagrass was 678.300 m2. Pari island has depth range between 0-50 metres and the physics and chemical parameter of water have a good range where the seagrass can live. According to the analysis result of STSI, suitable areas for seagrass transplantation donor is station no. 5 and 6 for seagrass species of Enhalus acoroides and station no. 2 and 7 for seagrass species Thalassia hemprichii. The implication of this research is that the seagrass needs rehabilitation to overcome seagrass ecosystem for better condition.
High utilization of fossil fuel increases the level of carbon dioxide in the atmosphere and results in global warming phenomenon. These things establish the world’s thought to look for the other alternative energy that can reduce the use of fossil fuel even to be replaced by the substitute. Recently, Indonesia has been doing the research of microalgae as a feedstock of an alternative biofuel. Fatty acid content that microalgae have is also high to produce biofuel. The steps used in this research TRI PRARTONO; MUJIZAT KAWAROE; DAHLIA WULAN SARI; DINA AUGUSTINE
HAYATI Journal of Biosciences Vol. 17 No. 4 (2010): December 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.17.4.196

Abstract

High utilization of fossil fuel increases the level of carbon dioxide in the atmosphere and results in global warming phenomenon. These things establish the world’s thought to look for the other alternative energy that can reduce the use of fossil fuel even to be replaced by the substitute. Recently, Indonesia has been doing the research of microalgae as a feedstock of an alternative biofuel. Fatty acid content that microalgae have is also high to produce biofuel. The steps used in this research is a 7 days cultivation, harvesting, extraction using hexane, and fatty acid identification using Gas Chromatography of microalgae species. Fatty acid component in some species such as Chlorella sp., Scenedesmus sp., Nannochloropsis sp., and Isochrysis sp. is between 0.21-29.5%; 0.11-25.16%; 0.30-42.32%; 2.06-37.63%, respectively, based on dry weight calculation. The high content of fatty acid in some species of microalgae showed the potential to be the feedstock of producing biofuel in overcoming the limited utilization from petroleum (fossil fuel) presently.
Specific Growth Rate of Chlorella sp. And Dunaliella sp. According to Different Concentration of Nutrient and Photoperiod Mujizat Kawaroe; Tri Prartono; Adriani Sunuddin; Dahlia Wulan Sari; Dina Augustine
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol. 16 No. 1 (2009): Juni 2009
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.994 KB)

Abstract

Light and nutrient are factors that support the microalgae growth rate besides COB2B, temperature, and salinity. Microalgae growth of Chorella sp. and Dunaliella sp. were observed to determine the influences of different nutrient concentration and photo period. Microalgae cultivation was located at laboratory using 100 mL Erlenmeyer. The specific growth rate of microalgae was observed for different nutrient concentration and photo period of light treatments. Using Guillard/f2 nutrient, the highest specific growth rate for Chorella sp. was 0.227/d and 0.289/d for Dunaliella sp. The highest microalgae specific growth rate influenced different photo period was 0.39/d and 0.329/d, respectively. Finally, the highest specific growth rate for both cultivated species of microalgae was observed at 2V nutrient concentration and 24 hour period of light treatment.Keywords : Spesific growth rate, photoperiod, nutrient, Chlorella sp., Dunaliella sp.
Optimum Fermentation Process for Red Macroalgae Gelidium latifolium and Gracillaria verrucosa Mujizat Kawaroe; Dahlia Wulan Sari; Junkwon Hwangbo; Joko Santoso
Journal of Engineering and Technological Sciences Vol. 47 No. 6 (2015)
Publisher : Institute for Research and Community Services, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.eng.technol.sci.2015.47.6.7

Abstract

Red macroalgae have the potential to be processed into bioethanol due to their high carbohydrate and low lignin content. Gelidium latifolium and Gracilaria verrucosa are red macroalgae commonly found in Indonesian seas. Sometimes an over-supply of red macroalgae is rejected by the food industry, which opens up opportunities for others uses, e.g. for producing bioethanol. The objectives of this research were to analyze the influence of sulfuric acid concentration on hydrolysis of G. latifolium and G. verrucosa and to calculate the optimum fermentation process to produce bioethanol. G. latifolium and G. verrucosa were hydrolyzed using H2SO4 at concentrations of 1%, 2%, 3%, and 4%, at a temperature of 121 °C and a pressure of 1.5 bar for 45 minutes. The process of fermentation was done using Saccharomyces cerevisiae in anaerobic conditions for 4, 5, 6 and 7 days. The results show that the optimum H2SO4 concentrations to hydrolyze G. latifolium and G. verrucosa were 1% and 2% respectively. The number of S. cerevisiae cells in hydrolysate G. latifolium and G. verrucosa increased in the third adaptation. S. cerevisiae can convert sugar from G. latifolium and G. verrucosa into bioethanol through fermentation. The highest bioethanol yields were achieved on days five and six. Therefore, red macroalgae can be seen as a potential raw material for bioethanol production.
Laju Pertumbuhan Spesifik dan Kandungan Asam Lemak pada Mikroalga Spirulina platensis, Isochrysis sp. dan Porphyridium cruentum (Specific Growth Rate and Fatty Acid Content of Microalgae Spirulina platensis, Isochrysis sp. and Porphyridium cruentum) Mujizat Kawaroe; Tri Pratono; Ayi Rachmat; Dahlia Wulan Sari; Dina Augustine
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 17, No 3 (2012): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.695 KB) | DOI: 10.14710/ik.ijms.17.3.125-131

Abstract

Mikroalga merupakan pabrik sel yang mampu mengkonversi karbondioksida menjadi biofuel potensial, makanan serta bioaktif yang bernilai tinggi dengan bantuan sinar matahari. Tujuan penelitian ini adalah untuk menganalisis laju pertumbuhan spesifik dan kandungan asam lemak 3 jenis mikroalga yaitu S. platensis, Isochrysis sp., P. cruentum. Kultivasi mikroalga murni dilakukan di laboratorium selama 8 hari dengan media Guillard/F2. Proses ekstraksi dilakukan dengan menggunakan metode sokletasi serta pelarut n-heksan. Profil dan kandungan asam lemak mikroalga dilakukan dengan menggunakan kromatografi gas spektrofotometri massa (GC-MS). Hasil penelitian menunjukkan bahwa kepadatan mikroalga tertinggi terdapat pada hari ke-8. Laju pertumbuhan spesifik tertinggi terdapat pada P. cruentum, yaitu 0,37/hari. Spesies mikroalga yang memiliki total konsentrasi asam lemak paling tinggi adalah Isochrysis sp., (96,18%) dan yang terrendah adalah P. cruentum (34,73%). P. cruentum memiliki kadar MUFA tertinggi. Isochrysis sp. memiliki kadar asam lemak tidak jenuh ikatan poli (PUFA) yang lebih tinggi daripada S. platensis.Kata kunci: mikroalga, S. platensis, Isochrysis sp., P. cruentum, asam lemak, pertumbuhan spesifikMicroalgae is cell factory that is capable of converting carbon dioxide to potential biofuel, foods and high-value bioactive with sunlight assistance. The purpose of this study is to analyze the specific growth rate and fatty acid content of S. platensis, Isochrysis sp., P. cruentum. Cultivation of pure microalgae is conducted in laboratory for 8 days with media Guillard/f2. Extraction process is conducted using soxhletation method and hexane as the solvent. Fatty acid profile and content in microalgae is performed using gas chromatography mass spectrometry (GC-MS). The highest density of microalgae found at day 8 cultivation. The highest specific growth rate found in P. cruentum in 0.37/day. The highest and lowest total of fatty acid concentration found in Isochrysis sp. (96.18%) and P. cruentum (34.73%) respectively. P. cruentum has the highest levels of saturated fatty acids (SFA), whereas S. platensis has the highest levels of mono unsaturated fatty acid (MUFA). Isochrysis sp. has lower levels poly unsaturated fatty acid (PUFA) than S. platensis.Key words: microalgae, S. platensis, Isochrysis sp., P. cruentum, growth rate, fatty acid
Perubahan Luas Penutupan Padang Lamun Di Kepulauan Seribu, DKI Jakarta Mujizat Kawaroe; Indra Jaya; Indarto H. S.; Dahlia Wulan Sari; Sundari Wening W.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2641

Abstract

Seagrass is one of productive and economical marine ecosystem, but recently there are indications that this area is decreasing and every kind of damage is happening. The research was conducted to reveal the change of seagrass width area and to determine the suitable location for donor and acceptor of seagrass transplantation. Methods of research were collecting and analyzing the satellite image of the research location, bathymetri mapping and water quality parameter, and also determining environment charasteristics that suitable to be performed by counting Seagrass Transplantation Suitability Index (STSI). The results show that in year 1999-2004, the derivation area of seagrass was 678.300 m2. Pari island has depth range between 0-50 metres and the physics and chemical parameter of water have a good range where the seagrass can live. According to the analysis result of STSI, suitable areas for seagrass transplantation donor is station no. 5 and 6 for seagrass species of Enhalus acoroides and station no. 2 and 7 for seagrass species Thalassia hemprichii. The implication of this research is that the seagrass needs rehabilitation to overcome seagrass ecosystem for better condition.
Building the Capacity and Knowledge about Coral Rehabilitation of Tour Guides in Lemukutan Island Dahlia Wulan Sari; Achis Martua Siregar; Tia Nuraya; Elliska Murni Harfinda; Mordik Erdiansyah
MITRA: Jurnal Pemberdayaan Masyarakat Vol 5 No 2 (2021): MITRA: Jurnal Pemberdayaan Masyarakat
Publisher : Institute for Research and Community Services

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/mitra.v5i2.2853

Abstract

The coral reef ecosystem is one of the marine tourism attractions on Lemukutan Island, West Kalimantan. However, currently, some locations are in a relatively degraded condition, which should be addressed. One solution to this problem is coral transplantation activities involving tour guides. As part of the effort to rehabilitate the coral reef habitats, the Aquatic Resource Management Department, Faculty of Agriculture, Nahdlatul Ulama University, West Kalimantan, conducted community development activities to increase the capacity and knowledge of tour guides regarding coral reef ecosystems and coral transplantation techniques. This training was attended by fifteen tour guides from the Lemukutan Guide Group and ARTUNA (Anak Remaja Teluk Cina) Group. The participants were previously trained under PT Pertamina (Persero) and Yayasan Jejak Pesisir Nusantara. This training was carried out in three stages: (i) materials presentation and discussions, (ii) direct practice on the field, and (iii) evaluation. The post-test results showed that the training had increased participants' understanding of coral reef ecosystems and coral transplantation techniques by 40,00% and 33,33%, respectively.
KEANEKARAGAMAN HAYATI ZOOPLANKTON DI PULAU PELAPIS, KABUPATEN KAYONG UTARA Dahlia Wulan Sari; Achis Martua Siregar
JURNAL BORNEO AKCAYA Vol 8 No 2 (2022): Borneo Akcaya : Jurnal Penelitian dan Pengembangan Pelayanan Publik
Publisher : Badan Penelitian dan Pengembangan Provinsi Kalimantan Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51266/borneoakcaya.v8i2.236

Abstract

Zooplankton is an animal plankton that connects producers and secondary consumers in an aquatic ecosystem. Data related to the condition of the waters of Pelapis Island as one of the mariculture zone in West Kalimantan is currently still limited. The zooplankton community structure is one of the important things in the development of mariculture. Therefore, this study aims to present data related to the zooplankton community structure in Pelapis Island Waters as the basis for developing mariculture. Sampling was carried out using a plankton net and preserved using Lugol. Identification was carried out at the Nahdlatul Ulama University Laboratory, West Kalimantan. The analysis was conducted to determine the index of diversity, evenness, and dominance of zooplankton. The results showed that the abundance of zooplankton in the waters of Pelapis Island dominated by the sub-phylum Crustacea and fish eggs at 44.05±1.09% and 35.07±2.97%, respectively. The number of genera found in this study was nine genera, and 4 of them were crustaceans. Zooplankton diversity showed moderate to low values and high dominance at Station 8.
Analisis Kandungan Nitrat dan Fosfat di Perairan Parit Baru, Kubu Raya Kalimantan Barat Tia Nuraya; Dahlia Wulan Sari; Elliska Murni Harfinda
Jurnal Kelautan, Lingkungan, dan Perikanan Vol 3 No 2 (2022): MANFISH JOURNAL
Publisher : Jurusan Ilmu Kelautan dan Perikanan Politeknik Negeri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.093 KB) | DOI: 10.31573/manfish.v2i3.457

Abstract

Sungai merupakan perairan yang sangat dipengaruhi oleh kegiatan manusia terutama dari daratan. Unsur-unsur hara yang berasal dari daratan dan dari aktivitas manusia yang masuk ke dalam perairan menyebabkan daerah tersebut memiliki banyak kandungan unsur hara. Tujuan dari penelitian ini adalah untuk mengetahui kandungan nitrat (NO3 - ) dan fosfat (PO4 + ) di Perairan Parit Baru Kabupaten Kubu Raya Kalimantan Barat. Metode penelitian yang digunakan adalah metode deskriptif bersifat eksploratif yang diterapkan pada 3 titik lokasi penelitian yaitu Parit Derabak, Parit Sembin, dan Parit Cabang Kiri. Pengambilan sampel dilaksanakan pada tanggal 20 Januari 2022. Data yang dikumpulkan sebagai variabel ukur adalah nitrat dan fosfat beserta data parameter fisis di lapangan. Data yang diperoleh dianalisis di laboratorium mengunakan alat atau metode spectrophotometer. Konsentrasi nitrat yang diperoleh berkisar antara 0,6 mg/l – 1,8 mg/l dan fosfat antara 0,27 mg/l – 5,06 mg/l di ketiga lokasi. Berdasarkan hasil tersebut menyatakan bahwa parameter nitrat dan fosfat termasuk memenuhi standar baku mutu air kelas II sesuai dengan PP No.22 Tahun 2021 yang mana diperuntukan untuk sarana rekreasi air, pembudidayaan ikan air tawar, peternakan, dan mengairi pertanaman.