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Antimicrobial and Antioxidant Activities of Resins and Essential Oil From Pine (Pinus merkusii, Pinuso ocarpa, Pinus insularis) and Agathis (Agathis loranthifolia) Tillah, Mardho; Batubara, Irmanida; Sari, Rita Kartika
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8371

Abstract

The most common human pathogen that colonizes in a third of healthy people around the world are Staphylococcus aureus, and one of the materials allegedly able to overcome the pathogen is resin. Resin has been used in folk medicine for thousands of years to treat diseases. The antimicrobial activity of natural resins can be associated with a variety of organic compounds contained in them such as diterpenoids and triterpenoids. This research aimed to explore the antibacterial and antioxidant activities of Pinus merkusii, P. oocarpa, P. insularis, Agathis loranthifolia resins and essential oil. Resin was separated by distillation process to get essential oil and the residue was extracted using n-hexane, ethyl acetate (EtOAc), and methanol (MeOH). Antioxidant activity was performed by DPPH (1,1diphenyl-2-picryl hydrazyl) radical scavenging method. The antibacterial activity of resins and essential oil of the samples determined using the disc diffusion method against Staphylococcus aureus and Escherichia coli. The results showed that the yield of resin extract was ranging from 8.44 % to 95.56%. All extracts and essential oil could not inhibit E coli growth but inhibit the S. aureus growth. This experiment concluded that resin n-hexane extract from P.oocarpa was the most potent as antibacterial activity against S. aureus. All of the samples used had less potential antioxidant activity compared to positive control ascorbic acid. Result of this study show that pine resin from Indonesia has potential as an antibacterial agent.
Aktivitas Antioksidan dan Tabir Surya Ekstrak Daun Gyrinops verstegii (Antioxidant activity and Sunscreen of Gyrinops versteegii Leaf Extract) Wahyuningrum, Maeda; Sari, Rita K; Rafi, Mohamad
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 16, No 2 (2018): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Jurnal Ilmu dan Teknologi Kayu Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (38.454 KB)

Abstract

The aim of this study was to determine the yield and phytochemicals, antioxidant activity, and sunscreen of the Gyrinops versteegii leaf extracts. The leaf simplicia was extracted using soxhletation method with multilevel polarities of solvent (n-hexane, ethyl acetate, and metanol). The analysis of phytochemical extracts has been carried out the qualitatively and quantitatively. The antioxidant activity testing was performed in vitro through the effective concentration (EC50) extract in capturing DPPH radicals. Sunscreen activity has been done through testing sun protection factor (SPF). The result showed that the yield of n-hexane extract, ethyl acetate, and methanol extracts were 7.83, 5.46, and 6.77% respectively. The phytochemical analysis showed that the ethyl acetate and methanol extracts were strongly detected containing antioxidant compounds such as p-hydroquinone, flavonoid, and tannins with the total phenol of the ethyl acetate and methanol extracts were 3.40 and 4.27% respectively. The n-hexane extract detected contains weakly the antioxidant compounds with the total phenol was 0.45%. The methanol extract is the highest antioxidant activity (EC50 14.46 μg ml-1) and has ultra sunscreen activity (SPF>15).Keywords: antioxidant, extractive, G. versteegii, sunscreen
Revitalisasi konservasi tumbuhan obat keluarga (toga) guna meningkatkan kesehatan dan ekonomi keluarga mandiri di desa Contoh Lingkar Kampus IPB Darmaga Bogor Agus Hikmat; Ervizal A.M. Zuhud; . Siswoyo; Edhi Sandra; Rita Kartika Sari
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 2 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1386.154 KB)

Abstract

Medicinal plants and traditional medicine for along ago are important role in the health care, stamina maintain, and treat diseases. Therefore medicinal plants and traditional medicines have strong root in the part of community up to now. Research on revitalization of family medicinal plant (TOGA) conservation done at Kampong Pabuaran (Cibanteng village), and Kampong Gunung leutik ( Benteng village), results indicated that research locations have completely medicinal plants diversity for medicine all diseases of village communities mentioned. Number of medicinal plants found at Gunung Leutik and Pabuaran Sawah Kampong (Cibanteng and Benteng Villages) were 237 spesies, and 95 spesies often used by respondents mentioned villages. Species number of medicinal plants have potential to expand based on use value of these species treat main diseases of community villages Gunung Leutik and Pabuaran Sawah Villages (Benteng and Cibanteng) were 15 spesies, such as: sambiloto (Andrographis paniculata (Burm.F) Ness.), meniran (Phyl/anthus niruri L.), takokak (Solanum torvum L.), pegagan (Centella asiatica (L.) Urban.), temulawak (Curcuma xanthorrhiza Roxb.), jahe (Zingiber officinale-purpurea Rose.), jeruk nipis (Citrus aurantifolia (Christm & Panz) Swingle), binahong (Anredera cordifolia), mahkota dewa (Phaleria macrocarpus (Sheff). Boerl.), rosella (Hibiscus sabdariffa), pule pandak (Rauvolfia serpentine (L.) Benth. ex. Kurz.), sangitan (Sambucus javanica Reinw.), sirih (Piper betle L.), brotowali ( Tinospora crispa), and kenikir (Cosmos caudatus).
Pemanfaatan Kompos Tandan Kosong Kelapa Sawit untuk Meningkatkan Pertumbuhan Tanaman Kayu Putih (Melaleuca cajuputi) di Lahan Pasca Tambang Batubara Agus Ari Subagio; Irdika Mansur; Rita Kartika Sari
Jurnal Silvikultur Tropika Vol. 9 No. 3 (2018): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.9.3.160-166

Abstract

Compost of oil palm empty bunches can improve fertility and soil nutrient on ex-mining land. This study aimed to evaluate the effect of oil palm empty fruit bunches composted on growth of Melaleuca cajuputi on post-mining land. This research was conducted at field for 3 months. The application was done at 1 to 3 years plant age. A single factor of empty fruit bunch compost organized on RCBD with 4 treatments and 4 replication. The results showed the compost able to increase growth of M. cajuputi. The best application was K1 (5 kg plant-1) increased height of 1 and 2 years plant age respectively by 67.82 and 38.86%. K2 (7.5 kg plant-1) at 3 years old increased by 30.16%. While the highest yield of diameter increased was on K2 (7.5 kg plant-1) at 1 and 3 years plant age (46.05% and 30.85%). The 2 year plant age with dose K3 (10 kg plant-1) was increased of 32.09%. In general, the application of EFB compost such as K1 treatment (5 kg plants-1) was reached the best dose of 1 and 2 year plant age and K2 treatment (7.5 kg plant-1) gave the best response for 3 years of plant age.Keywords: empty fruit bunch, EFB, Melaleuca cajuputi, postcoal-mining land
Antioxidant and Tyrosinase Inhibitor Activities of Methanol Extracts of Acacia mangium Rita K Sari; Rahmi Utami; Irmanida Batubara; Anne Carolina; Salina Febriany
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 13, No 1 (2015): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.652 KB) | DOI: 10.51850/jitkt.v13i1.63

Abstract

Active compounds utilization of its extractives for cosmetic products increase the value added of mangium tree. The purpose of this study was to determine the antioxidant and an inhibitor tirosinase activities of methanol extract of the various parts of mangium tree. Phytochemical properties of the best extract were also anlayzed. Extraction was conducted by soxhletation in methanol for 12 hours. The antioxidant and tyrosinase inhibitory activities of the extracts were tested in vitro to radical of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and inhibition to tyrosinase enzyme. The results showed that leave resulted in the highest methanol ectract followed succesively by bark, heartwood, and sapwood the value of 10.7; 4.4; 2.5; and 0.9%, respectively. The highest antioxidant activity was bark extract and followed by leave, heartwood, and sapwood extracts with EC50 values respectively of 8.3; 26.7; 66.9; and 137.9 ppm. Only bark extract which classified as an active tyrosinase inhibitor with IC50 value of 257.8 ppm in the difenolase reaction. The IC50 value of the positive control (kojic acid) was 116.7 ppm. The other extracts relatively inactive as a tyrosinase inhibitor because their IC50 values > 1000 ppm. The qualitative analysis detect the methanol extract of mangium bark as the best extract containing phenolic compounds (phenol hirokinon, flavonoids, and tannins) and alkaloids which were thought to contribute to the high antioxidant and tyrosinase inhibitor activities.Keywords: Acacia mangium, antioxidant, extracts, tyrosinase inhibitor
Potential Antidiabetic and Anticancer Agents from the Inner bark Extractives of Mount Salak Forest Woods Rita K Sari; Wasrin Syafii; Nur Azizah; Juliasman Juliasman; Muhammad Fadli; Minarti Minarti
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 12, No 2 (2014): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.87 KB) | DOI: 10.51850/jitkt.v12i2.66

Abstract

The aim of this research was to determine the extract contents, antidiabetic and anticancer activities of the acetone extracts of the inner barks of beunying (Ficus fistulosa)/BE, hamerang (F. foxicaria)/HE, kilemo (Litsea cubeba)/KLE, kiseueur (Antidesma tetandrum)/KSE, kopo (Eugenia cymosa)/KOE, and pasang butarua (Quercus induta)/PBE from Mount Salak Forest. The phytochemical profile of the best extract as antidiabetic and anticancer agents was also determined. The investigation of antidiabetic and anticacer activities of this extracts was carried out through invitro inhibitory α-glucosidase test and toxicity test to Artemia salina. The content of acetone extract of the KSE, KOE, and BE contents were in the range of 4.3-7.8% (high), however that of the KLE, HE, and PBE contents were in the range of 3.0-3.9% (moderate). The acetone extract of the KSE was very active as α-glucosidase inhibitor (IC50 5.9 mg ml-1), the KLE, PBE, and BE were rated active with IC50 value 11.2, 17.2, and 43.2 mg ml-1 respectively, while the HE and KOE were inactive (IC50 > 100 mg ml-1). The acetone extract of the KSE was very toxic to A.salina (LC50 19.7 mg ml-1), these of the HE, KOE, and BE were toxic with LC50 value 79.5, 94.5, and 115.9 μg ml-1 respectively, while these of the KLE and PSE were inactive (LC50 > 250 mg ml-1). The most potential antidiabetic and anticancer agents was the acetone extract of KSE. The acetone extract of the KSE was detected with strong intensity containing alkaloids, flavonoids, and tannins.Keyword: anticancer, antidiabetic, α-glucosidase, innerbark extractives, Mount Salak Forest
Aktivitas Antimalaria Ekstrak Kayu Bidara Laut Wasrin Syafii; Rita K. Sari; Umi Cahyaningsih; Laela N. Anisah
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 14, No 1 (2016): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.698 KB) | DOI: 10.51850/jitkt.v14i1.6

Abstract

Penyakit malaria merupakan salah satu penyakit menular yang masih menjadi masalah utama kesehatan di Indonesia. Penelitian ini bertujuan untuk menetapkan rendemen zat ekstraktif kayu bidara laut dan aktivitas antimalarianya secara in vitro  terhadap Plasmodium falciparum serta menganalisis kandungan kimia ekstrak teraktifnya  Ekstrak kayu bidara laut dihasilkan dari maserasi dengan pelarut organik berkepolaran bertingkat (n-heksana, etil asetat, dan etanol). Hasil penelitian menunjukkan bahwa kadar ekstrak tertinggi terdapat pada ekstrak etanol (4,11%), diikuti dengan ekstrak etil asetat (1,24%) dan ekstrak n-heksana (0,55%). Berdasarkan uji aktivitas antimalaria, ekstrak etanol merupakan ekstrak teraktif (IC50 3,09 µg ml-1), sedangkan ekstrak etil asetat (IC50 81,38 µg ml-1) dan ekstrak n-heksana (IC50 889,30 µg m-1) tergolong  tidak aktif.  Hasil uji fitokimia secara kualitatif menunjukkan kelompok senyawa yang terkandung di dalam serbuk kayu bidara laut adalah flavonoid, alkaloid, triterpenoid, steroid, tannin dan hidroquinon yang diduga berperan terhadap aktivitas antimalaria. Analisis GCMS mendeteksi adanya senyawa alkaloid strychnine yang diduga memiliki aktivitas antimalaria. Berdasarkan hasil tersebut, ekstrak etanol kayu bidara laut sangat berpotensi sebagai sumber obat antimalaria.
Sifat Antirayap Resin Damar Mata Kucing dari Shorea javanica K. et V. Antitermic Properties of Resin from Shorea javanica K. et V Rita Kartika Sari; Wasrin Syafii; Kurnia Sofyan; Muhammad Hanafi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 2, No 1 (2004): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.077 KB) | DOI: 10.51850/jitkt.v2i1.314

Abstract

This study was undertaken primarily to isolate and identify substances from resin of Shorea javanica K. et. V. that might be responsible to inhibit the termite activity of Coptotermes curvignathus Holmgren. Approximately 500 gram of dried-resin was extracted with acetone solvent. The acetone extract was then fractionated into n-hexane, diethyl ether, ethyl acetate, and insoluble fraction (residue). The no-choice bioassay test was carried-out by treating paper discs with extracts at  the concentration of  2.0%, 4.0%, 6.0%, 8.0%, 10.0%, 12.0% (W/W), and 0.0 % as control. The bioassay test showed that n-hexane and diethyl ether soluble fraction exhibited highest antitermic properties at Lethal Concentration (LC)50 value of 1.62% and continuation fraction showed N3 of n-hexane fraction an EE1 of diethyl ether fraction exhibited highest toxicity at LC50 value of 1.23 % and 1.65%. Further investigation of the n-hexane soluble fraction by using CC, GC-MS, FTIR, UV-Vis, and NMR led to the isolation and identification of the main compound, namely friedelin, while the diethyl ether soluble fraction contains vulgarol B; 3,4-Secodamar-4(28)-en-3-oic acid; (7R,10S)-2,6,10-Trimethyl-7, 10–epoxy-2,11-dodecadien; and junipene (decahydro-4,8,8-trimethyl-1,4-methanoazulene)
Aktivitas Antidiabetes Ekstrak Etanol Jabon (Anthocephalus cadamba) (Antidiabetic Activity of Jabon (Anthocephalus cadamba) Ethanol Extracts) Laela N. Anisah; Wasrin Syafii; Rita K. Sari; Gustan Pari
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 13, No 2 (2015): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.939 KB) | DOI: 10.51850/jitkt.v13i2.28

Abstract

Diabetes mellitus (DM) merupakan penyakit gangguan metabolik yang menjadi masalah utama kesehatan di dunia termasuk Indonesia. Penelitian ini bertujuan untuk menetapkan rendemen zat ekstraktif tanaman jabon,  aktivitas antidiabetesnya secara in vitro  terhadap enzim -glukosidase serta menganalisis kandungan kimia ekstrak teraktifnya   Ekstrak jabon dihasilkan dari proses maserasi dengan etanol 95% pada berbagai bagian pohon (daun, kulit, kayu). Hasil penelitian menunjukkan bahwa kadar ekstrak tertinggi terdapat pada bagian daun (16,5%), diikuti bagian kulit (4,62%) dan kayu (2,04 %). Berdasarkan uji aktivitas penghambatan enzim   -glukosidase, ekstrak etanol daun jabon merupakan ekstrak teraktif dengan nilai IC50 7,24 µg ml-1 (sangat aktif), sedangkan ekstrak etanol bagian kulit dan kayu tergolong  tidak aktif (IC50 > 100  µg ml-1). Hasil uji fitokimia secara kualitatif menunjukkan kelompok senyawa yang terkandung di dalam ekstrak etanol daun jabon adalah flavonoid, hidroquinon, saponin, tannin, alkaloid, terpenoid dan steroid yang  diduga  berperan  dalam  menghambat  aktivitas  enzim    -glukosidase.  Analisis  GCMS mendeteksi adanya senyawa fenolik asam quinat dan katekol serta turunan asam lemak (asam heksadekanoat metil ester) yang diduga memiliki aktivitas antidiabetes. Berdasarkan hasil tersebut, ekstrak etanol daun jabon sangat berpotensi sebagai sumber obat andiabetes.
Stabilitas Fisikokimia dan Sifat Antipenuaan Kulit Formula Krim Berbahan Aktif Alami (Physicochemical stabilities and Skin Antiaging Properties of Bioaktive-based Cream Formulas) Rita K Sari; Nyoman J Wistara; Deded S Nawawi; Nopen Meisaroh; Ietje Wientarsih; D R Agungpriyono; L N Sutardi; Mawar Subangkit; Vetnizah Juniantito
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 15, No 1 (2017): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (525.345 KB) | DOI: 10.51850/jitkt.v15i1.366

Abstract

 The purpose of this study was  to analyze the physicochemical stabilities  and in vivo antiaging  properties of the cream formulas of F1, F2, and F3 which contained the active compound of 0.1% 0.18, and 0.36%, respectively. The active compound was the combination of  ethyl acetate fraction of  Toona sinensis, Centella asiatica, and Acacia mangium  leaf (1:2:1). For physicochemical stability tests (color, odor, acidity, and antioxidant activity), the creams were stored for 21 days in the refrigerator, room temperature,   and extreme temperature (±40 ⁰C). For in vivo antiaging test,  the mice skin  were smeared with cream and UV irradiated for 15 minutes per day. After two and four weeks, the mice skins were  biopsied. The results showed that the physicochemical properties of creams stored in the refrigerator were  stable. The storage of creams at room and  extreme temperature decreased the physicochemical properties.  The application of cream formulas on mice skin after two to four weeks were able to increase the thickness of epidermis, but has not been able to increase the collagen skin of mice.Keywords: Acacia mangium, Centella asiatica, ethyl acetate fraction of leaf, physicochemical and antiaging properties of cream, Toona sinensis