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Gunarti Gunarti
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GOLD IMMUNOCHROMATOGRAPHIC ASSAY (GICA) SEBAGAI IMUNOSENSOR MENDETEKSI ANTIBODI Bacillus Anthracis PENYEBAB PENYAKIT ZOONOSIS Gunarti Gunarti; Yunan Jiwintarum; Awan Dramawan; Lale Budi Kusuma Dewi
Jurnal Kesehatan Prima Vol 9, No 1 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.272 KB) | DOI: 10.32807/jkp.v9i1.64

Abstract

Abstract: The purpose of this study was to identify patterns of protein antigens full / secretory antigens may be antigenic protein (PA) of Bacillus anthracis by the use of SDS-PAGE method (Laemmli) which can be used as bioreseptor on the test device fabrication method for detecting antibodies GICA of Bacillus anthracis , identify patterns of proteins that are antigenic protein (PA) or indicate the nature of the reactivity of Bacillus anthracis by using Western blotting and Determining the specificity and sensitivity of the test method to detect antibodies GICA Bacillus anthracis when using the results of the ELISA method as a reference. This study is a descriptive observational study. Research variable antigenic protein of Bacillus anthracis as bioreseptor and Antibodies from Bacillus anthracis as imunosenssor. The test prototype test device made using 20 sample with ELISA as a reference for calculating the sensitivity and specificity. The results of this study were identified band – band of 92 kDa, 68 kDa, 54 kDa, 43 kDa, 29 kDa and 18 kDa that can be used as bioreseptor on GICA, identified a pattern of proteins that are antigenic or indicate the nature of the reactivity of the Bacillus anthracis 68 kDa by Western blotting using the method, extract secretory antigen (SA) Bacillus anthracis can be used as a test device bioreseptor on GICA method for the detection of IgG antibodies against Bacillus anthracis and sensitivity and specificity of the test method to detect antibodies GICA Bacillus anthracis when using the results of the ELISA method as a reference each is 100% and 87.5%.
THE IMMUNE RESPONSE IN WORM INFECTION AT PUSKESMAS TANJUNG KARANG IN MATARAM Gunarti Gunarti; Erlin Yustin Tatontos; Urip Urip
Jurnal Kesehatan Prima Vol 12, No 2 (2018): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.272 KB) | DOI: 10.32807/jkp.v12i2.189

Abstract

Abstrac: A worm infection can decrease sufferers’ health condition, nutrition, intelligence and their productivity thus, it can cause numerous of disadvantages economically. The immune response to worm infection begins with Th2 activation with a significant increase of IL-4, IL-5, IL-9, IL-10 and IL-13. The cytokines IL-5 that formed stimulates the growth and activation of eosinophils. The purpose of this research was to know the immune response by increasing the number of eosinophils to worm infection rate at Puskesmas Tanjung Karang. The research design was expost facto with cross sectional or transversal. The sample was communities’ faces and blood at Puskesmas Tanjung Karang as many as 50 people. The technique sampling was random from family members who have worm risk factors (poor sanitation conditions, living near animal cage, ground floor houses, pets such as dogs and cats). The correlation analysis of worm infection with eosinophils used Mann Whitney Test. The results showed that the prevalence of worm infection belong to mild category for Trichurin trichura was 10%, and the overall worm infection was 12% whereas the average number of eosinophils in the normal category was between 50-300 cells / mm². The results of statistical analysis were not significant (Asymp. Sig. 0.059) which means that there was no effect of the immune response with the number of eosinophils on the level of worminfection at Puskesmas Tanjung Karang.Keywords: Immune response, eosinophil, worm infection.
Co-Authors Awan Dramawan Erlin Yustin Tatontos Lale Budi Kusuma Dewi Urip Urip Yunan Jiwintarum