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All Journal Meditory : The Journal of Medical Laboratory Jurnal Kesehatan Prima
Lale Budi Kusuma Dewi
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Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Nursing Public Health

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GOLD IMMUNOCHROMATOGRAPHIC ASSAY (GICA) SEBAGAI IMUNOSENSOR MENDETEKSI ANTIBODI Bacillus Anthracis PENYEBAB PENYAKIT ZOONOSIS Gunarti Gunarti; Yunan Jiwintarum; Awan Dramawan; Lale Budi Kusuma Dewi
Jurnal Kesehatan Prima Vol 9, No 1 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.272 KB) | DOI: 10.32807/jkp.v9i1.64

Abstract

Abstract: The purpose of this study was to identify patterns of protein antigens full / secretory antigens may be antigenic protein (PA) of Bacillus anthracis by the use of SDS-PAGE method (Laemmli) which can be used as bioreseptor on the test device fabrication method for detecting antibodies GICA of Bacillus anthracis , identify patterns of proteins that are antigenic protein (PA) or indicate the nature of the reactivity of Bacillus anthracis by using Western blotting and Determining the specificity and sensitivity of the test method to detect antibodies GICA Bacillus anthracis when using the results of the ELISA method as a reference. This study is a descriptive observational study. Research variable antigenic protein of Bacillus anthracis as bioreseptor and Antibodies from Bacillus anthracis as imunosenssor. The test prototype test device made using 20 sample with ELISA as a reference for calculating the sensitivity and specificity. The results of this study were identified band – band of 92 kDa, 68 kDa, 54 kDa, 43 kDa, 29 kDa and 18 kDa that can be used as bioreseptor on GICA, identified a pattern of proteins that are antigenic or indicate the nature of the reactivity of the Bacillus anthracis 68 kDa by Western blotting using the method, extract secretory antigen (SA) Bacillus anthracis can be used as a test device bioreseptor on GICA method for the detection of IgG antibodies against Bacillus anthracis and sensitivity and specificity of the test method to detect antibodies GICA Bacillus anthracis when using the results of the ELISA method as a reference each is 100% and 87.5%.
UJI KUALITATIF ALKOHOL URINE PEMINUM TUAK DALAM MENGETAHUI WAKTU SAMPLING YANG TEPAT I Made Suardika; Lale Budi Kusuma Dewi; Thomas Tandi Manu; I Wayan Getas; Ida Bagus Rai Wiadnya
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): meditory,Volume 11 No. 1, 2023
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2421

Abstract

In making a diagnosis of the influence of alcohol on urine for a crime and the occurrence of traffic accidents, it is necessary to take the right sampling time. The influencing factor in determining the results of toxicological analysis is the time of sampling. In addition, another factor to consider when detecting alcohol in urine is the time of last consumption. This study was conducted to determine the appropriate urine sampling time for alcohol consumption. The method used in this urine alcohol qualitative test uses the modified 2.5% potassium bichromate method in 50% H2SO4. The sample consisted of 10 respondents who drank palm wine after 1 hour, 2 hours and 3 hours of consumption. The qualitative test results of urine drinkers of palm wine drinkers after 1 hour, 2 hours and 3 hours of consuming. 10 respondents obtained the results of 4 positive urine samples 1 hour after consuming and 2 positive urine samples 3 hours after consuming palm wine, as well as sampling in the 2nd hour the test results were negative. The positive test results depended on the habit of consuming, the volume consumed, and the age of the wine drinker.
Co-Authors Awan Dramawan Gunarti Gunarti I Made Suardika I Wayan Getas Ida Bagus Rai Wiadnya Ida Bagus Rai Wiadnya Goes Rai Lidia Febrianisa Thomas Tandi Manu Yunan Jiwintarum