Claim Missing Document
Check
Articles

Found 29 Documents
Search

Perbedaan Tingkat Kecemasan antara Siswa Sekolah Menengah Pertama Reguler dan Akselerasi di Sekolah Menengah Pertama Negeri 1 Wonogiri Oktafiani, Anindya; Susilohati, Mardiatmi; Sari, Yulia
Nexus Pendidikan Kedokteran dan Kesehatan Vol 1, No 1 (2012): Nexus Pendidikan Kedokteran & Kesehatan
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.273 KB)

Abstract

Background: Anxiety is the apprehension of something that will occur with uncertain causes. Class program differences may cause anxiety. In acceleration program, student are expected to be more critical and creative given limited and targeted study time, but will likely be more anxious at a time due to tight competition. This research aims to find out whether anxiety level differs between regular students and accelerated students at SMP Negeri 1 Wonogiri. Method: This study was a descriptive analytical research using cross sectional approach conducted in March 2012 at SMP Negeri 1 Wonogiri. Subjects were purposively sampled based on the inclusion and exclusion criteria and were subsequently randomly sampled. Subjects filled in (1) curriculum vitae form, (2) L-MMPI questionnaire to find out the respondents truthfulness in filling in the questionnaire, and (3) T-MAS questionnaire to measure the anxiety score. Subjects were excluded when their L-MMPI scores were ≥ 10 or did not fill in the questionnaire completely. Data were analyzed by Chi-square test using SPSS 17.0 for Windows. Result: Analysis from eighty subjects showed a X2 value of 0.341, (p=0.599), meaning that there was no difference of anxiety level between regular and accelerated students at SMP Negeri 1 Wonogiri. Conclusion: There was no evidence of anxiety level differences between regular and accelerated students iat SMP Negeri 1 Wonogiri. Keywords: Regular program, Acceleration program, Anxiety
Hubungan Pemberian Obat Anti Tuberkulosis (OAT) dengan Kadar Asam Urat Setiawan, Ivan; Setijadi, Ana Rima; Sari, Yulia
Nexus Kedokteran Klinik Vol 3, No 1 (2014): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.046 KB)

Abstract

Background: Multidrug tuberculosis treatment which is a long term medication often causes various side effects. One of them is an increase of uric acid level. This study aims to determine the correlation between tuberculosis treatment administration and uric acid level. Methods: This study used cohort method with one group before and after intervention design. A total of 30 tuberculosis patients who were admitted to Center of Lung Health Society Surakarta between April and August 2013 were taken as samples by consecutive sampling method. Criteria of sampling are pulmonary tuberculosis patients who is planned to get rifampisin, isoniazid, pirazinamid, and ethambutol for more than 4 weeks, aged over 18 years old, and had given informed consent. In this study we excluded pulmonary tuberculosis patients who also had liver or renal disorders, had suffered from hyperuricemia or took drugs for decreasing uric acid level, or had hypertension. The measurements of uric acid level was collected before the treatment and more than one  month after the treatment. Data were analyzed by paired t test (α = 0,05). Results: The average uric acid level of 30 patients before treatment was 4.68 ± 1.12 mg/dL, while the average uric acid levels after treatment was 8.56 ± 2.21 mg/dL, which was significantly higher than pretreatment (p=0.001). During treatment, hyperuricemia (uric acid level > 7 mg/dL) was reported in 73.33% (22/30) of patients and two patients had arthralgia. Conclusions: Tuberculosis treatment administration may affect uric acid level. Hiperuricemia was reported in 73.33% of patients who receive tuberculosis treatment. Keywords: tuberculosis treatment, uric acid level, tuberculosis 
Analisis Molekuler Regio Pre-S1, Pre-S2, dan S Isolat Virus Hepatitis B 09IDSKAB-3 Prasetyo, Angga Dwi; Prasetyo, Afiono Agung; Sari, Yulia
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: The Hepatitis B Virus (HBV) pre-S1, pre-S2, and S region are essentially known for virus infection and replication. Eight genotypes (A to H) and nine subtypes (adw2, adw4, ayw1, ayw2, ayw3, ayw4, adrq+, adrq-, and ayr) of HBV have been identified worldwide. The aim of this study was to analyze HBV genetic variation in pre-S1, pre-S2, and S region, and to determine genotype and subtype of 09IDSKAB-3 HBV isolate from Men Who Have Sex With Men community in Surakarta. Methods: 09IDSAKAB-3 HBV DNA extraction was used as a template for amplication of pre-S1, pre-S2, and S region. The sequence results were then aligned by Clustal W with all of reference sequences reported in GenBank/DDBJ/EMNL. Genetic variation in pre-S1, pre-S2, and S region were identified using MEGA 4.0. HBV genotype and subgenotype were identified by phylogenetic analysis. HBV subtype was deduced on the basis of the predicted amino acid sequences of HBsAg. Results: Based on BLAST search in GenBank, 09IDSKAB-3 isolate was classified into genotype B3. D27E variation was found in pre-S1 region, and there were not genetic variation in pre-S2 and S region. Phylogenetic tree showed that 09IDSKAB-3 isolate was classified into genotype B3. Based on the basis of predicted amino acid sequences of HBsAg, 09IDSKAB-3 isolate was classified into subtype adw2. Conclusions: Overall, only D27E variation was found in this study. 09IDSKAB-3 isolate was classified into genoytpe B3 and subtype adw2. The amino acid variations found in the present report need further study. Keywords: HBV, pre-S1 region, pre-S2 region, S region
Analisis Molekuler Regio Core Promoter dan Precore/CoreIsolat Virus Hepatitis B 09IDSKAB-3 Yudistiro, Ibnu; Prasetyo, Afiono Agung; Sari, Yulia
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: HBV replicates its DNA genome through reverse transcription from RNA intermediate. It is vulnerable to a high number of mutations during such reverse transcription which are frequently found in core promoter and precore/core regions. This study was aimed to identify genetic variation of HBV core promoter and  precore/core regions of 09IDSKAB-3 isolate. Methods: DNA extraction was performed on 09IDSKAB-3 blood sample that was taken from Man Sex with Man Community. Core promoter and precore/core regions were determined by PCR using KL-28 and KL-6 primers and direct sequencing of the corresponding region. Molecular analysis was performed using MEGA 4.0. Results: Based on BLAST result, 09IDSKAB-3 HBV isolate had the highest similarity to isolate AP011085 from DKI Jakarta. Genetic variations A1726C in core promoter, and T1860C, C1877T, G1957C in precore/core region were found in 09IDSKAB-3 isolate. Conclusions: 09IDSKAB-3 HBV isolate was classified into genotype B and subgenotype B3 based on core promoter and precore/core region. The genetic variations found in this isolate may have influence to the replication efficiency and HBeAg/HBcAg production, therefore need further study. Keywords: hepatitis B virus, molecular analysis, core promoter region, precore/core region
Efek Nefroprotektor Ekstrak Biji Mahoni (Swietenia mahagoni (L.) Jacq.) terhadap Kerusakan Histologis Sel Ginjal Mencit (Mus musculus) yang Diinduksi Parasetamol Muvida, Muvida; Muthmainah, .; Sari, Yulia
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Seed extract of Swietenia mahagoni (L.) Jacq. contains saponins, flavonoids, alkaloids, terpenoids, steroids, and tannins that may exhibit significant protection of kidney cells from free radicals. In present study, Swietenia mahagoni was evaluated for its nephroprotector effect on paracetamol-induced kidney cells histological damage in mice (Mus musculus).   Methods: This research used experimental laboratory studies with post test only controlled group design. Samples were 28 male Swiss webster mice (2-3 months old) weighing + 20 g and divided into 4 groups, 7 mice each group. Sampling technique in this research was incidental sampling. The negative control group (KK (-)) and the positive control group (KK (+)) of mice were administered aquadest for 14 days. The first treatment group (KP1) and the second group treatment (KP2) of mice were administered mahagony seed extract with the dose of 11,2 mg/20 g and 22,4 mg/20 g body weight of mice for 14 days, respectively. Paracetamol was administered to groups of KK (+), KP1, dan KP2 on the 12th, 13th, and 14th day. On day-15th, mice were sacrificed and their kidneys were taken and stained with hematoxilin eosin (HE) for histological study. The kidney cells histological features were assessed based on quantifying of pyknosis, karyorrhexis, and karyolysis. Data were analyzed with the One-Way ANOVA test (α = 0.05) and Post Hoc Multiple Comparisons LSD test (α = 0.05). Results: The mean of kidney cells histological damage in mice for KK (-), KK (+), KP1 and KP2 were 9.57 + 0.701; 28.93 + 1.698; 9.28 + 0.873 and 27.79 + 1.651, respectively. Result of statistic analysis showed that there were significant differences of kidney cells damage score between KK (-) – KK (+), KK (-) – KP2, KK (+) – KP1, KP1 – KP2, and non significant differences between KK (-) – KP1 and KK (+) – KP2. Conclusions: Swietenia mahagoni (L.) Jacq. seed extract showed nephroprotector effect against paracetamol-induced kidney cells histological damage in mice. Keywords: mahagony seed extract, nephroprotector, kidney cells histological damage
Efek Proteksi Biji Mahoni (Swietenia mahagoni Jacq.) terhadap Kerusakan Hepar Mencit yang Diinduksi Parasetamol Endahfuri, Dahniar; Listyaningsih, Endang; Sari, Yulia
Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.205 KB)

Abstract

Background: Seedof Swietenia mahagoni Jacq. contains alkaloids, saponins, flavonoids, triterpenoids, steroids, and tannins which were thought to protect liver from free radicals.In present study, Swietenia mahagony Jacq.seed extract was evaluated for its protective effect on paracetamol-induced liver damage in mice. Methods:To evaluate the hepatoprotective effects of Swietenia mahagony Jacq. seed extract, 28 male Swiss webster micewere equally divided into four groups. Animals of group I (K) and group II (P1) were given aquadest for 14 days. Group III (P2) received 14 mg/25 g body weight of mice of Swietenia mahagony Jacq. seed extract and the last group (P3)received 28 mg/25 g body weight of mice of Swietenia mahagony Jacq. seed extract for 14 days.Paracetamol was given to groups of P1, P2, andP3.Mice were sacrificedon the 15th day and histological preparation was made to evaluate histological damage on liver. Liver histological features were assessed by counting the number of hepatocyte on centrolobuler zone undergoing pyknosis, karyorhexis, and karyolysis. Data were analyzed using the OneWay ANOVA test (α = 0.05) and then were continued with Post Hoc Multiple Comparisons (LSD) test (α = 0.05). Results:The mean liver histological damage score was significantly higher on group of P1=60.71+7.521, whilegroup of K= 9.86+1.574 as the less.P2grouphad fewer numbers of liver histological damage (32.57+2.573) compared to the P3group(43.57+3.101). The results of OneWay ANOVA and LSD test showed that there was significant difference between the four groups (p = 0.000). Conclusions:Swietenia mahagony Jacq.seed extract showed protective effect against the hepatotoxicity induced by paracetamolin mice.Swietenia mahagony Jacq.seedextract with the dose of 14 mg/25 g bodyweight of mice showed a higher hepatoprotective effect than the dose of 28 mg/25 g body weight of mice. Keywords:Swietenia mahagony Jacq. seed, liver damage,paracetamol
Ko-infeksi Virus Hepatitis B dan Virus Hepatitis C pada Penderita HIV/AIDS di Surakarta, Indonesia Sidhajati, Raden Artheswara; Prasetyo, Afiono Agung; Sari, Yulia
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: One problem in HIV treatment is HBV/HCV co-infection due to their similarity in the transmission routes. HBV/HCV co-infection is believed increasing the risk of hepatotoxicity, hepatocellular carcinoma, steatosis, fibrosis, and also rapidly lead to AIDS in the HIV patients. The purpose of this study was to find out the status of HBV/HCV co-infection among HIV patients in Surakarta. Methods: In November-December 2011 all of HIV patients (n=65) visited the Voluntary Counseling and Testing in Moewardi General Hospital Surakarta were involved in the study. Clinical data and blood samples were retrieved from all patients. Serological assays to detect the presentation of HBsAg and anti-HCV were performed in all samples. Results: There were 17% (11/65) HIV patients infected with the hepatitis viruses. Six point two percent (4/65) was HBsAg+, 10.8% (7/65) was anti-HCV+, and 0% with triple infection. There were 57.1% (4/7) females and 42.8% (3/7) males detected with anti-HCV+. Males and females, each was found 50% (2/4) in HBsAg+. Ninety one percent (10/11)HIV patients with both HBV or HCV co-infection were under 50 years old .None of routine blood test was significant with HBsAg+ or anti-HCV+. Conclusions: Several HIV patients had co-infection with Hepatitis B Virus and Hepatitis C Virus, and most of them were females under 30 years. HIV patients should be checked the status of HBV and HCV co-infection in order to have a better therapeutic management. Keywords: HIV co-infection, Hepatitis B Virus, Hepatitis C Virus
Pengaruh Pemberian Ekstrak Etanol Bit Merah (Beta vulgaris L.) Terhadap Kerusakan Histologis Sel Hepar Mencit (Mus musculus) yang Diinduksi Parasetamol Pangestika, Debora Marga; Suyatmi, .; Sari, Yulia
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Red beet (Beta vulgaris L.) has various components such as betalain, flavonoid, phenolic, β-karoten, vitamin C and E, Fe, Zn, Cu, Mn, and Selenium. These components are potent to be antioxidant to protect liver from free radicals. The aims of this research were to evaluate hepatoprotective effect of ethanol extract of red beets on histologic damage of mice’s liver cells induced by paracetamol and the dose dependent of this extract. Methods: This was laboratory experimental research with the posttest only controlled group design. The subjects of this reaseach were 30 Swiss webster male mice (Mus musculus), 2-3 months old and ± 20 grams of weight, divided into 5 groups randomly. The groups were KK(-), KK(+), KP1, KP2, and KP3. Aquadest was administered to KK(-) and KK(+). Paracetamol was administered to KK(+), KP1, KP2, and KP3 on the day 12, 13 and 14. Red beet extracts were administered to KP1, KP2, and KP3 in dose 5.6 mg,  11.2 mg and 22.4 mg per 20 g BW of mice, successively. The hepatoprotective effects were assessed by counting the number of damage cells among 100 hepatocytes at central lobe zone of liver. Results: The result of One-Way ANOVA p = 0.000 (p < 0.05) showed a significant difference among each other group. The linear regression test p = 0.000 (p < 0.05) showed a correlation between increasing dosage and improving protective effect on mice’s liver cell damage. Conclusions: The ethanol extract of red beet had protective effect on histologic damage of mice’s liver cells induced by paracetamol. The increasing dosage of the red beet extract improved the protective effect to liver cells. Keywords : ethanol extract red beet, mice liver cells, paracetamol 
Pengaruh Pemberian Ekstrak Etanol Bit Merah (Beta vulgaris) terhadap Kerusakan Histologis Sel Ginjal Mencit (Mus musculus) yang Diinduksi Parasetamol Anggara, Amanda Yessica; Muthmainah, .; Sari, Yulia
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Kidney failure can happen due to intoxication of paracetamol that causes oxidative stress. Red beet (Beta vulgaris) containing various components such as vitamin A, C and E, flavonoid, Cu, Zn, Mn, selenium, betanin, and phenolic has a function as antioxidants. The aims of this research were to know the nefroprotective effect of ethanol extract of red beet to prevent renal cell damage of mice. Methods: This was laboratory experimental research with posttest only controlled group design. Subjects of this research were thirty Swiss webster male mice (Mus musculus), 2-3 months old and ± 20 grams of body weight (BW), each.. The subjects were divided into 5 groups randomly. The groups of this research were negative control (KK(-)), positive control (KK(+)), experimental group 1 (KP1), experimental group 2 (KP2), and experimental group 3 (KP3). The KP1, KP2 and KP3 were administered 5.6 mg, 11.2 mg and 22.4 mg per 20g BW, successively. The nefroprotective effect of ethanol extract of red beet to prevent renal cell damage of mice was assessed by counting the number of damage cells among 50 renal cells on pars convulata. Results: One-Way ANOVA analysis p=0.000 (p<0.05) followed by Post Hoc Multiple Comparisons showed a significant difference between negative control and other groups. The increasing dose of the ethanol extract of red beet was followed by the improvement of the protective effect. The linear regression test p=0.000 (p<0.05) showed a correlation between the increase of dose of ethanol extract of red beet and the improvement of the nefroprotective effect to the histological damage of mice’s renal cells. Conclusions: Ethanol extract of red beet showed the nefroprotective effect to the histological damage of mice’s renal cells induced by paracetamol. The increase of dose of the ethanol extract of red beet improved the protective effect to mice’s renal cells. Keywords: ethanol extract of red beet, histological damage of mice’s renal cells, paracetamol 
Efek Nefroprotektif Ekstrak Etanol Buah Jambu Biji Merah (Psidium guajava Linn) terhadap Kerusakan Histologis Ginjal Mencit yang Diinduksi Asetaminofen Priscilla, Prisca; Suyatmi, .; Sari, Yulia
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.207 KB)

Abstract

Background: Red guava fruit (Psidium guajava Linn) contains various of antioxidants such as vitamins A, C, E, minerals Fe, Zn, Se, flavonoids, and lycopene, which they were thought to have nephroprotective effect. The objective of this research was to evaluate the preventive effect of red guava fruit ethanolic extract on tubular renal damage induced by acetaminophen on mice. Methods: Samples were twenty eight male mice, Swiss webster strain, 2-3 months old age and + 20 gram of each weight. Sample divided into 4 groups, each group consists of seven mice. The negative (KK(-)) and positive control group (KK(+)) mice were given aquadest for 14 days. The other group of mice were given red guava fruit ethanolic extract with the dose of 35 mg/20 g body weight of mice (KP1) and the last group of mice were given red guava fruit ethanolic extract with the dose of 70 mg/20 g body weight (KP2) for 14 days. Acetaminophen was given to groups of KK(+), KP1, and KP2. The 15th day, mice were sacrificed and histological preparation were made to evaluate histological damage on rens. Renal histological features were assessed by counting the number of tubular epithelial undergoing pyknosis, karyorrhexis, and karyolysis. Data were analyzed using the One-Way ANOVA test (α = 0.05) and then were continued with Post Hoc Multiple Comparisons (LSD) test (α = 0.05). Results: The mean renal histological damage score was significantly higher on group KK(+)= 40.50+1.991, while the group of KK(-)= 8.93+1.720 as the less. The group of KP2 had fewer numbers of renal histological damage (17.96+1.621) compared to the KP1 group (25.36+1.929). The results of One-Way ANOVA and LSD test showed that there was significant difference between the four groups (p = 0.000). Conclusions: The ethanolic extract of red guava fruit showed nephroprotective effect to the renal histological damage of mice which was induced by acetaminophen. Red guava fruit ethanolic extract with the dose of 70 mg/20 g body weight of mice showed a higher nephroprotective effect than the dose of 35 mg/20 g body weight of mice. Keywords: ethanolic extract, red guava, acetaminophen, renal histological, mice.