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Delesi Gen APOBEC3B pada Pasien Human Immunodeficiency Virus di RSUD Dr. Moewardi Surakarta Kindi, Ekkim Al; Prasetyo, Afiono Agung; Sari, Yulia
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background:HIV infection on human can get inhibition from intrinsic factor. Human can produce antivirus protein which is encoded by APOBEC3 gene. APOBEC3B (A3B) is one of APOBEC3 protein, which cannot be degradated by HIV vif protein. Therefore A3B protein is a potent inhibitor of HIV replication. But, A3B gene can get a deletion, which can decrease its effect in inhibiting HIV infection.The aim of this study was to find correlation between CD4 cells count in A3B gene deletion-positive respondents and A3B gene deletion-negative respondents. Methods: There were 51 HIV patients in Dr. Moewardi General Hospital Surakarta on November 2011, which were included as our respondents. We take respondentsâs blood and were isolated their DNA. Then, we run in PCR with deletion primer (Deletion_F dan Deletion_R) to detect if there was a deletion in A3B gene sequence. We run electrophoresis in 1% agarose gel with Loading Quick Ð¤X174/HaeIII 72-1353bp as a marker. Then, we visualized the gel on Gel Documentation and was interpreted. Data of A3B gene deletion, respondentsâs description, HIV RNA detection, and CD4 cell count were analysed with Chi Square. Results:About 36 respondents (70.6%) were positive for deletion in A3B gene. Four of them were positive for HIV RNA detection. The average of CD4 cells count in respondents, who were positive for A3B gene deletion, was 426.86 Â± 407.4 cells/Âµl and the others were 496.93 Â± 573.0 cells/Âµl (p = 0.782). There were no correlation (p > 0.050) between A3B gene deletion in female respondents and CD4 cells count. A3B gene deletion was more likely to be found in female respondents (OR = 2.286; 95% CI : 0.669 â 7.808). Conclusions:There were no differences between the average of CD4 cell counts in A3B gene deletion-positive respondents and A3B gene deletion-negative respondents. Keywords:Polymorphism, Deletion, APOBEC3B, HIV Â

Perbedaan Tingkat Kecemasan Antara Siswa yang Mengikuti Dan tidak Mengikuti Bimbingan Belajar Dalam Menghadapi Ujian Nasional Di SMA Negeri 5 Surakarta Parendra, G. Harldy; Hadin, Yusvick; Sari, Yulia
Nexus Kedokteran Klinik Vol 3, No 3 (2014): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Anxiety is the signal awakening, warning the presence of treating hazard and enabling an individual to take some measures to deal with the threat. National exam is one factor that can result in anxiety in the students. Learning guiding is one of learning infrastructures out of school time functioning to help the student practice solving the problem quickly. This research aimed to find out whether or not there is a difference of anxiety level between students attending and those not attending Learning Guiding in Facing National Exam. Â Method: This study was an analytical descriptive research using cross sectional approach. This research was conducted in SMA Negeri 5 Surakarta. The sample was taken using purposive sampling followed by random sampling. The sample was divided into 2 groups: the students attending and those not attending learning guiding. The students then filled in (1) personal data form, (2) L-MMPI questionnaire, (3) TMAS questionnaire, if the students did not meet inclusive and exclusive criteria and result of L-MMPI questionnaire â¥ 10, the sample cannot be chosen. The-60 samples obtained were analyzed using Chi Square using SPSS program 17.00 for Windows Program. Result: This research showed the c2 Chi-Square value of 1.684, while p = 0.194 or p > 0.05 meaning that there was no difference of anxiety level between the students attending and those not attending learning guiding in facing National Exam. Conclusion: There was no difference of anxiety level between the students attending and those not attending learning guiding in facing National Exam. Keywords: Learning Guiding, Anxiety.Â

Cloning and Sequencing cDNA Encoding for Rhoptry-2 Toxoplasma Gondii Tachyzoite Local Isolate Artama, Wayan T.; Sari, Yulia; Subekti, Didik Tulus; Poerwanto, Soenarwan Hery; Subandono, Jarot
Indonesian Journal of Biotechnology Vol 10, No 2 (2005)
Publisher : Universitas Gadjah Mada

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Rhoptry protein belongs to an excretory and secretory antigens (ESAs) that play an important role during activepenetration of parasite into the cell target. This protein an able Toxoplasma gondii to actively penetrate targetedcell, meanwhile ESAs protein stimulates intracellular vacuole modification. It is, therefore, after the parasitesuccessfully enter the cell target then Granule (GRA) proteins are responsible for the formation of parasitophorusvacuole, which is protect the fusion with other intracellular compartments such as lysosomal vacuole. Consequently,this parasite is being able to survive and multiply at the cell target. The current study was aimed to clone andsequens cDNA encoding for ROP-2 of local isolated T. gondii tachizoite through DNA recombinant technique.Total ribonucleic acid (RNA) was isolated from tachyzoites of local isolated T. gondii that were grown up in Balb/c mice. Messenger RNA was isolated from total RNA using PolyAtract mRNA Isolation System. Messenger RNA wasused as a template for synthesis cDNA using Riboclone cDNA Synthesis System AMV-RT. EcoRI adaptor fromRiboclone EcoRI Adaptor Ligation System was added to Complementary DNA and than ligated to pUC19. Recombinantplasmid was transformed into E. coli (XL1-Blue). The transformed E. coli XL-1 Blue were plated on LB agarcontaining X-Gal, IPTG and ampicillin. Recombinant clones (white colony) were picked up and grown up in theLB medium at 37oC overnight. Expression of recombinant protein was analysed by immunoblotting in order toidentify cDNA recombinant wich is express ESA of T. gondii local isolate. Recombinant plasmid were isolatedusing alkalilysis method and were elektroforated in 1% agarose gel. The isolated DNA recombinant plasmid wascut using Eco RI and then sequenced through Big Dye Terminator Mix AB1 377A Sequencer using M13 Forward andM13 Reverse primers. The conclusion of this results showed that the recombinant clone was coding for excretoryand secretory protein which has molecular weight of 54 kDa. The DNA alignments of sequence from the clonedgene showed 97% homology with gene encoding for ROP-2 of T. gondii RH isolate.Keywords: Toxoplasma gondii, tachizoite, ESA, complementary DNA, ROP2

Pengaruh Pemberian Jus Stroberi (Fragaria x ananassa) Terhadap Kerusakan Histologis Sel Hepar Mencit yang Diinduksi Rhodamin B Pratiwi, Risky; Muthmainah, .; Sari, Yulia
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Strawberry contains flavonoid, anthocyanin, ellagic acid and vitamin C that can protect the liver from free radicals. The aims of this research are to know the effect of strawberry juice and the increasing of its dose on the liver histological damage of mice caused by rhodamine B. Methods: This study was laboratory experimental research with the post-test only control group design. The laboratory animal used in this experiment was male Swiss webster mice with 2-3 months old and Â± 20 grams of each weight. Twenty eight samples were divided into 4 groups (KKn, KP, KP1 and KP2), each group consisted of 7 mice. The sampling technique in this research was consecutive sampling. The normal control group (KKn) was given distilled water, while the exposure group (KP), the 1st treatment group (KP1), and 2nd treatment group (KP2) were given rhodamine B. The mice of KP1 and KP2 were given strawberry juice in dose 0.4 ml/20 gWt and 0.8 ml/20 gWt. These treatments were done for 16 days, then on the 17th day mice were killed with neck dislocation and their livers were made for preparation with paraffin block method and stained with HE. Liver cell histological damages were assessed based on quantifying necrotic cells of each 100 cells in the first zone and third zone. The data were analyzed using One-Way ANOVA test and Post Hoc LSD test (Î± = 0.05). Results: The data showed mean of necrotic cells in KKn, KP, KP1 and KP2 were 38.64 Â± 2.56; 157.64 Â± 2.87; 90.07 Â± 2.70; and 68.93 Â± 2.56, repeatedly. The results of One-Way ANOVA test showed p = 0.000. The results of LSD test showed significant difference between KKn-KP, KKn-KP1, KKn-KP2, KP-KP1, KP-KP2, and KP1-KP2 with p = 0.000 for each groups. Conclusion: Strawberry juice reduces miceâs liver cell histological damage caused by rhodamine B and the increasing dose of strawberry juice can reduce liver cell histological damage. Keywords: Strawberry juice, rhodamine B, liver cell histological damageÂ Â

Pengaruh Pemberian Ekstrak Etanol Daun Kenikir (Cosmos caudatus Kunth.) terhadap Kerusakan Struktur Histologis Hepar Mencit (Mus musculus L.) yang Diinduksi Parasetamol Irawan, Alvian Oscar; Suyatmi, .; Sari, Yulia
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Kenikir leaf contains flavonoids, phenolic acid, saponin, carotenoids, vitamin A, vitamin C, Fe, Zn, and Cu. They are potential antioxidants being capable of protecting cells from free radical. The aim of this research is to evaluate the hepatoprotective effect of kenikir (Cosmos caudatus Kunth.) leafâs ethanol extract on mice induced by paracetamol. Methods: This research was a laboratory-based experiment with the post-test only with control group design. The samples of 35 male Swiss webster mice, aged 2-3 months with Â± 20 gram of weight were randomly divided into 5 groups. The groups were normal group (KN), negative control group (KK(-)), treatment group 1 (KP1), treatment group 2 (KP2), and treatment group 3 (KP3). Extract dosage of 2.8 mg, 5.6 mg, and 11.2 mg were administered daily for 14 days to KP1, KP2 and KP3, respectively. A toxic dosage of paracetamol was administered to KK(-), KP1, KP2, and KP3 on day 12th, 13th, and 14th. On day 15th, mice were sacrificed by neck dislocation and the liver were prepared and stained with HE for histology study. The degree of damage being due to the paracetamol was based on summation of pyknosis, karyorrhexis, karyolysis nucleus. Data were analyzed using One-Way ANOVA test (Î± = 0.05), followed by Post Hoc Multiple Comparisons (LSD) (Î± = 0.05) test. Results: The One-Way ANOVA test result was p = 0.000 (p < Î±). The statistical LSD test result was p = 0.000 (p < Î±), which showed significant differences in the hepatic damage between KN-KK(-), KN-KP1, KN-KP2, KN-KP3, KK(-)-KP1, KK(-)-KP2, KK(-)-KP3, KP1-KP2, KP1-KP3, and KP2-KP3 group. Conclutions: The administration of kenikir leafâs extract is able to prevent the damage in miceâs liver cells induced by paracetamol. Keyword: ethanol extract, kenikir (Cosmos caudatus Kunth.) leaf, paracetamol, liver cells, miceÂ

Pengaruh Ekstrak Etanol Daun Kenikir (Cosmos caudatus Kunth.) terhadap Kerusakan Struktur Histologis Ginjal Mencit (Mus musculus L.) yang Diinduksi Parasetamol Wibowo, Lina Kristanti; Suyatmi, .; Sari, Yulia
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Kenikir leaves contain various kind of antioxidants, such as vitamin C, Î²-carotene, minerals, and polyphenols which are known to protect kidneys from oxidative stress caused by free radical exposure. The purpose of this study is to evaluate the nephroprotective effect of Kenikir leaf extract toward histological damage of the mices kidney cells induced by Parasetamol. Methods: This was a laboratory experimental study with the posttest only controlled group design. The samples were 35-Swiss webster male mice, aged 2-3 months, and weight about 20 gram. Samples were divided into five groups randomly. Normal group (KN) was given distilled water and Negative control (KK(-)) was given Parasetamol. Extract dosage of 2.8 mg, 5.6 mg, and 11.2 mg of kenikir leaves were given daily for 14 days to treatment group 1 (KP1), treatmen group 2 (KP2), and treatment group 3 (KP3), respectively. A toxic dosage of Parasetamol as much as 5.07 mg, was given to negative control group and treatment groups on day 12th, 13th, and 14th. On day 15th , mice were sacrificed by neck dislocation. The kidneys were taken for histological preparation and stained with HE. The histological damage was assessed based on quantifying the pyknosis, karyorrhexis, and karyolysis on 50 proximal tubuli of epithelial cells. The data were analyzed using One-way ANOVA test (Î± = 0.05) and Post Hoc Multiple Comparisons test (Î± = 0.05). Results: The most damaged cells were found in KK(-) and the least in KN. The significant difference was observed among groups (One-way ANOVA test p = 0.000). The significant difference was observed between groups (Post Hoc test p = 0.000). They were KN-KK(-), KN-KP1, KN-KP2, KN-KP3, KK(-)-KP1, KK(-)-KP2, KK(-)-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusions: Ethanol extract of Kenikir leaves can prevent the histological damage of the mices kidney cell induced by Parasetamol. The increasing dose of the extract is followed by the increase of protective effect. Keywords: Kenikir leaves, histological damage, mices kidney, ParasetamolÂ

Koinfeksi Virus GB Tipe C (GBV-C) Pasien Human Immunodeficiency Virus (HIV) Rumah Sakit Umum Daerah Dr. Moewardi di Surakarta Sariyatun, Ratna; Prasetyo, Afiono Agung; Sari, Yulia
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Epidemiological data of GB Virus-C (GBV-C) in Indonesia are limited. Information about GBV-C coinfection among HIV patients in Dr. Moewardi General Hospital in Surakarta has also been unknown. Thus, this study is aimed to know the status of GBV-C coinfection among HIV patients in Dr. Moewardi General Hospital in Surakarta. Methods: Blood samples from 44 HIV patients in Dr. Moewardi General Hospital were subjected to viral RNA isolation. Complementary DNA (cDNA) was synthesized from the isolated RNA and used as template for nested polymerase chain reaction (PCR) detecting the 5-non-translated region of GBV-C. The amplicons were electrophoresed in 2% agarose gel and then visualized under UV and interpreted. Results: GBV-C RNA was detected in seven samples from HIV patients (15.9%, 7/44). All patients with GBV-C coinfection were women. Conclusions: The frequency of GBV-C RNA coinfection among HIV patients in Dr. Moewardi General Hospital in Surakarta is 15.9% (7/44). Keywords: GBV-C, HIV, SurakartaÂ

ANALISIS IMUNOGENISITAS PROTEIN GRA1 DARI HASIL KLONING GEN GRA1 TAKIZOIT Toxoplasma gondii [Immunogenicity Analysis of GRA1 Protein Derived from Clone Bearing GRA1 Genes Collected from Toxoplasma gondii Tachyzoite] Subekti, Didik T; Artama, WT; Poerwanto, SH; Sulistyaningsih, E; Sari, Yulia
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The study was aimed to analyze the immunogenicity of GRA1 protein derived from clone bearing GRA1 genes from local isolate of Toxoplasma gondii. Analysis of GRA1 protein translated from cDNA of GRA1 is very essential in prior to expressed the gene. Analysis of GRA1 protein derived from clone bearing GRA1 genes was performed using several bioinformatics software which are available as standalone or online software such as CLC Bio Workbench series, BioEdit, BESTORF, GENSCAN, FGENES, BepiPred 1.0, CTL Epitope Finder and SignalP. Translation coding sequences of GRA1 gene into GRA1 peptide sequences revealed 190 amino acids with molecular mass of GRA1 approximately 20.159 kD and isoelectric point at 4.43. GRA1 protein also identified several antigenic domains with six domains were known as epitopes for CD8+/cytotoxic lymphocyte and seven domain as epitopes for B lymphocyte. However, GRA1 protein was considered as good antigen but less immunogenic.

FABRIKASI AWAL PENUMBUHAN LAPISAN TIPIS CARBON NANOTUBES DENGAN METODE VHF-PECVD Kurniasih, Sri Cicih; Sari, Yulia
Jurnal Riset Industri Vol 2, No 2 (2008):
Publisher : Badan Penelitian dan Pengembangan Industri

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Saat ini carbon nanotubes merupakan Â salah satu topik nanoteknologi yang Â menarik Â di Â dunia Â karenaÂ memiliki sifat elektronik dan mekanik yang sangat unik dan menarik banyak perhatian untuk diaplikasikan. Fabrikasi awal Â penumbuhan Â lapisanÂ tipis Â carbon Â nanotube Â telah Â dilakukan dengan Â metode Â Very HighÂ Frequency Â Plasma Â Enhanced Â Chemical Vapour Deposition (VHF-PECVD) pada frekwensiÂ Â 70 MHz. Sumber karbon yang digunakan adalah gas metan (CH4) sedangkan sumber hidrogen adalah silan (SiH4)Â Â Â Perbandingan antara Â laju aliran metan dan silan Â 10: Â 1 dan tekanan 400 mTorr. Laju deposisi optimum akan diperoleh dengan menvariasikan daya rf dari 20 watt - Â 50 watt yang berperan sebagai sumber pembangkit plasma. Penumbuhan dilakukan pada suhu 250 DC dengan waktu deposisi selama 80 menit. Identifikasi lapisan tipis dilakukan menggunakan SEM danÂ EDX. Butiran-butiran dengan diameter sekitar 0,1 Âµm.-0,5 Âµm.(100 nm - Â 500 nm), telah tumbuh Â diatas permukaan substrat meskipunÂ belum terbentuk tubeÂ tube yang mengindikasikan carbon nanotube. Komposisi lapisan tipis dengan karbon terbesar dan silikon yang seminimal mungkin terjadi pada daya rf 40 watt yaitu atom carbon 56,25 %, atom silikon 37,22 %Â Â Kata kunci Â : Carbon nanotube , VHFPECVD, Â daya rf,Â Â Â SEM-EDX.

FABRIKASI AWAL PENUMBUHAN LAPISAN TIPIS CARBON NANOTUBES DENGAN METODE VHF-PECVD Kurniasih, Sri Cicih; Sari, Yulia
Jurnal Riset Industri Vol 2, No 2 (2008):
Publisher : Badan Penelitian dan Pengembangan Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4780.146 KB)

Saat ini carbon nanotubes merupakan Â salah satu topik nanoteknologi yang Â menarik Â di Â dunia Â karenaÂ memiliki sifat elektronik dan mekanik yang sangat unik dan menarik banyak perhatian untuk diaplikasikan. Fabrikasi awal Â penumbuhan Â lapisanÂ tipis Â carbon Â nanotube Â telah Â dilakukan dengan Â metode Â Very HighÂ Frequency Â Plasma Â Enhanced Â Chemical Vapour Deposition (VHF-PECVD) pada frekwensiÂ Â 70 MHz. Sumber karbon yang digunakan adalah gas metan (CH4) sedangkan sumber hidrogen adalah silan (SiH4)Â Â Â Perbandingan antara Â laju aliran metan dan silan Â 10: Â 1 dan tekanan 400 mTorr. Laju deposisi optimum akan diperoleh dengan menvariasikan daya rf dari 20 watt - Â 50 watt yang berperan sebagai sumber pembangkit plasma. Penumbuhan dilakukan pada suhu 250 DC dengan waktu deposisi selama 80 menit. Identifikasi lapisan tipis dilakukan menggunakan SEM danÂ EDX. Butiran-butiran dengan diameter sekitar 0,1 Âµm.-0,5 Âµm.(100 nm - Â 500 nm), telah tumbuh Â diatas permukaan substrat meskipunÂ belum terbentuk tubeÂ tube yang mengindikasikan carbon nanotube. Komposisi lapisan tipis dengan karbon terbesar dan silikon yang seminimal mungkin terjadi pada daya rf 40 watt yaitu atom carbon 56,25 %, atom silikon 37,22 %Â Â Kata kunci Â : Carbon nanotube , VHFPECVD, Â daya rf,Â Â Â SEM-EDX.

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