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. Sarsono
Biochemistry Laboratory, Medical Faculty of Sebelas Maret University, Surakarta

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Perbedaan Kadar Quercetin pada Propolis Isolat Gunung Lawu antara yang Diekstrak dengan Etanol dan Propylene Glycol Endraputra, Pristiawan Navy; Prasetyo, Diding Heri; Sarsono, .
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Propolis contains quercetin, a substance from flavonoid group which is usually found as its glycoside form and its polarity changes to polar character. More polar of the solvent used, the greater flavonoid compound obtained. Consequently, it leads to different amount of quercetin gotten by different solvents on propolis extraction method. Method: This research used the post test only group design. Aluminium chloride colorimetric assay was applied to determine the amount of quercetin obtained from both ethanolic and propylene glycol extracting procedures. The data were statistically analyzed by independent t-test method. The propolis was collected from Kerjo, Karanganyar. Result: Total dissolved quercetin in ethanolic extract and propylene glycol extract were 63.38 ± 13.14 µg/mL and 45.69 ± 8.70 µg/mL, each, p < 0.05. Conclusion: This research significantly showed that the quercetin level of propolis extracted with ethanol higher than the one extracted with propylene glycol. Keywords: Aluminium Chloride, Propolis, Quercetin 
Uji Ekstrak Daun Kumis Kucing Dalam Menurunkan Kadar Alanin Transaminase pada Mencit yang Diinduksi Minyak Goreng Pemanasan Berulang Meidiana, Steffi; Prasetyo, Diding Heri; sarsono, .
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Backgrounds: Orthosiphon stamineus Benth leaves contain various antioxidants like flavonoids, organic acids, and terpenoids. The main active chemical substances which are derived from flavonoids, namely sinensetin, and non-flavonoids, namely rosmarinic acids, have antioxidants activities as free radical scavengers. This study aimed to determine the effects of Orthosiphon stamineus Benth leaves extract in decreasing the levels of ALT in male strain Swiss webster mice induced by reheated cooking oil. Methods: This study was an experimental laboratory with a post test only controlled group design. The samples were 25 male strain Swiss webster mice, 2-3 months old and + 25-30 grams weight, each. Samples were taken incidentally sampling, arranged into 5 groups randomly, consisted of 5 mice each group. The control group (K) was administered distilled water and treatment group 1 (P 1) were administered reheated cooking oil. While treatment group 2 (P 2), treatment group 3 (P 3) and treatment group 4 (P 4) were consecutively administered 3.5 mg, 7.0 mg and 14.0 mg/25 gr BW of Orthosiphon stamineus Benth leaves extract and reheated cooking oil for 14 days. On the 15th day, blood samples were drawn from the mice through plexus retroorbitalis using microtubuler tube then their ALT levels were measured. Data were analyzed using One-way ANOVA (α = 0.05) and followed by Post Hoc Multiple Comparisons test (LSD) (α = 0.05). Results: Mean rate of alanin transaminase level in group K, P 1, P 2, P 3 and  P 4 were  54.600 + 7.765 U/L;  – 95.000 + 23.537 U/L; 62.200 + 7.463 U/L; 60.400 + 6.427 U/L and 57.400 + 10.922 U/L, in a row. Conclusions: Orthosiphon stamineus Benth leaves extract decreased alanin transaminase level in mice induced reheated cooking oil. Keywords: ALT, Orthosiphon stamineus Benth leaves extract, reheated cooking oil.