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INDUKSI KALUS KACANG TANAH (Arachis hypogaea L.) VARIETAS KELINCI DENGAN PERLAKUAN 2,4-D DAN BAP Ida Royani; Lalu Zulkifli; Prapti Sedijani
Jurnal Penelitian Pendidikan IPA Vol. 1 No. 2 (2015): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v1i2.21

Abstract

Callus induction determines the successfulness of micropropagation through somatic embryogenesis. This research was conducted to determine the effect of 2,4-D and BAP on callus induction of peanut (Kelinci strain). Callus induction was done on MS medium, with the observed variables were of initiation time, diameter, texture and color callus. Statistical analysis showed that 2,4-D and BAP affect the initiation time, diameter, texture and color callus. The earliest initiation of callus formation was obtained from MS medium with the addition of 4 mg/L 2,4-D. The diameter of the largest callus obtained from MS medium with the addition of 3 mg/L 2,4-D + 1 mg/L BAP. Callus color were affected by the treatment of 2,4-D and BAP, while callus texture was not affected by these treatmen, in which compact callus texture was not affected by these treatment, in which compact callus texture was observed in all treatments.Keywords: Induction of callus, 2,4-D, BAP, peanuts
EFEKTIFITAS BEBERAPA TANAMAN HIAS DALAM MENYERAP TIMBAL (Pb) DI UDARA Baiq Mirawati; Muhlis -; Prapti Sedijani
Jurnal Penelitian Pendidikan IPA Vol. 2 No. 1 (2016): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v2i1.32

Abstract

The objective of this reasearch was to determine the ornamental plants Lidah mertua (Sanseviera trifasciata.sp), Puring (Codiaeum variegatum), Palem Kuning (Chrysalidocarpus lutecsens) ability to absorb lead (Pb) concentration. This research is descriftive explorative research design with completely randomized design with 9 times treatments and 3 repetitions. The independent variables were lidah mertua (Sanseviera trifasciata.sp) Puring (Codiaeum variegatum), Palem Kuning (Chrysalidocarpus lutecsens), and the subjec variable is measurement of lead (Pb) concentration in the leaves. Data analysis techniques using qualitative and quantitative analysis. Quantitative data was obtained by calculating the lead (Pb) concentration in ornamental plants such as: Lidah mertua (Sanseviera trifasciata.sp) Puring (Codiaeum variegatum), Palem Kuning (Chrysalidocarpus lutecsens) by using AAS (Atomic Absorption Spectrophotometer) test.  Based on reasearch finding, there several finding of oranamental plants ability in absorb lead (Pb): lidah mertua (Sanseviera trifasciata.sp ): 0,06 ppm; 0,1 ppm; 0,15 ppm, puring (Codiaeum variegatum):  0,04 ppm; 0,09 ppm; 0,2 ppm and palem kuning (Chrysalidocarpus lutecsens): 0,02 ppm; 0,04 ppm; 0,11 ppm. Statistical test states that the type of plant and the traffic density does not affect the ability of plants to absorb lead (Pb).Keywords: Lead (Pb), lidah mertua (Sanseviera trifasciata.sp), Puring (Codiaeum variegatum), Palem Kuning (Chrysalidocarpus lutecsens).
INDUKSI KALUS TANAMAN KRISAN (Chrysanthemum morifolium) DENGAN PEMBERIAN BENZIL AMINO PURIN (BAP) DAN DICHLOROFENOKSI ACETIL ACID (2,4 D) Maspan Hariyati; Imam Bachtiar; Prapti Sedijani
Jurnal Penelitian Pendidikan IPA Vol. 2 No. 1 (2016): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v2i1.37

Abstract

Production of chrysant flowers (Chrysanthemum morifolium) is halted due to seedling supplay, that it is necessary to produce seedling on tissue culture. This study aims to determine the effect of BAP and 2.4 D for callus formation of chrysanthemum. The research using completely randomized design (CRD). The treatment was consistsed of two factors. BAP (0,0 mg/l, 1,0 mg/l, 1.5 mg/l and 2,0 mg/l) and concentration of 2,4 D (0,0 mg/l, 2,0 mg/l, 3,0 mg/l, 4,0 mg/l). Dependent variables measured were days of callus occurrence, callus colour and texture of callus. Result showed that growth regulators BAP and 2.4 D influenced the timing of the callus formation. Most rapid callus formation appeared at concentration of 2,0 mg/ l 2,4 D and longest callus formation appeared at concentrations of 0,0 mg/l 2,4 D. Callus did not appear on treatment of 0,0 mg/l BAP. The combination of BAP and 2.4 D did not influence on colour and texture of callus. Highest callus score was obtained in the treatment combination of D0B1, D0B0,5 and D0B2. The lowest callus score obtained in the treatment D4B0.Keyword: BAP, 2,4 D, Chrysanthemum morifolium, explants, Callus.
UJI MEKANIK MATERIAL KOMPOSIT SERAT PINANG Pathul Hapiz; Aris Doyan; Prapti Sedijani
Jurnal Penelitian Pendidikan IPA Vol. 4 No. 2 (2018): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v4i2.53

Abstract

The research aim to identify the mechanical properties of  Areca nut fiber composite.. Pinangs fiber composite board uses polyester resin as a binder. Areca nut fiber that used in this research is fiber obtained from pinang which is old and not used anymore. The sample was made by varying the concentration of fiber used (30%, 40%, 50% 60% and 70%). From the results of tests conducted, there are differences in mechanical properties of pine fiber composite material on tensile test and press tests performed. In tensile test, composite of pinang fiber with 50% fiber concentration is the fiber with the best mechanical properties because it has the highest tensile strength, maximum length and maximum load, that is 16.35 MPa, 2.06 mm and 3147.45 N. Meanwhile, Composite betel fiber with a concentration of 30% has the highest broken modulus (MOR) at 30.43 kg / cm2, and elastic module of 1980.2 MPa
UJI AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BUAH JERUK NIPIS (Citrus aurantifolia Swingle) TERHADAP PERTUMBUHAN BAKTERI ISOLAT KLINIS Rohmi Wardani; Dwi Soelistya Dyah Jekti; Prapti Sedijani
Jurnal Penelitian Pendidikan IPA Vol. 5 No. 1 (2019): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v5i1.101

Abstract

The objective of this research was to test antibacterial activity ethanol and ethyl acetate of lime peel (Citrus aurantifolia Swingle) extract by Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) against the growth of clinical isolate bacteria. The type of this study used laboratory experimental using sensitivity test, dilution of tube and spreading plate. The experimental design used Complete Random Design using three repetitions on three concentrations i.g 25, 50, and 75%. Data was analyzed by qualitative with measuring inhibit diameter zone bacterial and qualitative using Two Way Anova followed by BNT with α= 5%. The statistical analysis showed that the solvent factor and concentration factor was significantly against growing bacterial and there was an interaction affect of both factors on S. aureus growth. The result presented that the ethanol and ethyl acetate of lime peel extract strongly inhibited Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa; and midly inhibited the growth of Klebsiella pneumoniae. The MIC ethanol and ethyl acetate of lime peel extract against S. aureus, S. epidermidis and P. aeruginosa was 25% and K. pneumoniae was 50%. The MBC showed that ethanol and ethyl acetate of lime peel  extract killed bacteria P. aeruginosa at 15%, S. aureus, S. epidermidis at 20% and K. pneumoniae at 25%. Taken together, it can be concluded that ethyl acetate of lime extract more effective than that of ethanol extract was to inhibit and killed clinical isolate bacterial.Keywords: lime peel (Citrus aurantifolia Swingle), antibacterial activity, clinical isolate bacteria, MIC and MBC.
Peran Trehalose Metabolisme Sepanjang Masa Kehidupan Tanaman Prapti Sedijani
Jurnal Biologi Tropis Jurnal Biologi Tropis. Vol.14 No. 2 Desember 2014
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (688.977 KB) | DOI: 10.29303/jbt.v14i2.141

Abstract

AbstractTrehalose/T6P has important role at entire plant stage of life starting from embryonicstage, vegetative growth, generative growth and senescence. The roles include celldevision, cell shape, plant architectecture, and plant responses to biotic and abiotic stress.Syncrunizing between energy demand and energy availability is the main mode of theroles through reprogramming of gene expression responsible for anabolism and/orcatabolism processes depending on given situation including biotic and abiotic stress.Moreover, proteins of trehalose metabolism are suggested to affect enzyme activity oftarget proteins. SnRK1 and bZIP11 involve in all those modes. Understanding crusialroles of trehalose metabolism in plant therefore is necessary in order to get benefit fromthis pathway for agricultural issue.AbstrakTrehalose/T6P berperan penting bagi tahapan kehidupan tanaman dari tahap embryo,pertumbuahn vegetatif, pertumbuhan generatif, hingga sanesensi. Peran molekul tersebutberpengaruh pada pembelahan sel, bentuk sel, arsitektur tanaman dan respon tumbuhanterhadap stres biotik dan abiotik. Pengintegrasian antara kebutuhan dan ketersediaanenergi merupakan modus utama dalam peran tersebut melalui pemrograman ekspresi genyang bertanggung jawab terhadap proses anabolisme dan atau katabolisme sesuai dengansituasi tertentu termasuk dalam kondisi stres biotic dan abiotik. Selain itu, protein-proteindalam lintasan metabolisme trehalose ini juga diduga mempengaruhi aktivitas enzimenzimtarget. Peran krusial metabolisme trehalose, manusia dapat memanfaatkan lintasanini untuk kepentingan pertanian.
Peran Trehalose Metabolisme Sepanjang Masa Kehidupan Tanaman Prapti Sedijani
Jurnal Biologi Tropis Jurnal Biologi Tropis. Vol.15 no.2 Desember 2015
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.633 KB) | DOI: 10.29303/jbt.v15i2.204

Abstract

ABSTRAKTrehalose/T6P berperan penting bagi tahapan kehidupan tanaman dari tahap embryo, pertumbuahn vegetative, pertumbuhan generatif, hingga sanesensi melalui keterlibatannya dalam menentukan pembelahan sel, bentuk sel, arsitektur tanaman dan respon terhadap stress biotik dan abiotik. Mekanisme trehalose/T6P dalam peran tersebut melibatkan SnRK1 dan bZIP11 dengan mengintegrasikan antara ketersediaan dan penggunaan sumber karbon yang mempengaruhi pemrograman ekspresi gen-gen yang trerkait dalam anabolisme dan katabolisme serta gen-gen yang terlibat dalam respon tanaman terhadap stres biotik dan abiotik. Selain itu, protein-protein dalam lintasan metabolisme trehalose diduga terlibat juga dalam pemrograman dan aktivitas enzim-enzim target. Dengan mengetahui peran krusial metabolisme trehalose, manusia dapat memanfaatkan lintasan ini untuk kepentingan pertanian.   The Roles of Trehalose Metabolism at Entire Plant Life Abstract Trehalose/T6P has important role at entire plant stage of life starting from embryonic stage, vegetative growth, generative growth and senescence. The roles include cell devision, cell shape, plant architectecture, and plant responses to biotic and abiotic stress. Syncrunizing between energy demand and energy availability is the main mode of the roles through reprogramming of gene expression responsible for anabolism and/or catabolism processes depending on given situation including biotic and abiotic stress. Moreover, proteins of trehalose metabolism are suggested to affect enzyme activity of target proteins. SnRK1 and bZIP11 involve in all those modes. Understanding crusial roles of trehalose metabolism in plant therefore is necessary in order to get benefit from this pathway for agricultural issue. 
Penanaman Seribu Pohon Sebagai Upaya Mencegah Banjir dan Tanah Longsor di Desa Eyat Mayang, Lembar, Nusa Tenggara Barat Prapti Sedijani; Miko Eniarti; Nurwidianti; Siska Yulia Hermana; Jumriani
Jurnal Pengabdian Magister Pendidikan IPA Vol 5 No 2 (2022): April-Juni
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.186 KB) | DOI: 10.29303/jpmpi.v5i2.1837

Abstract

Eyat Mayang is a hilly Village where landslides can occur during heavy rains. The rainfall makes the dike unable to accommodate the overflow from the river. This condition is exacerbated by the unavailability of a Garbage Final Destination (TPA), causing the community to throw their garbage in the river. In addition, silting of the river naturally occurs. Garbage piling and river siltation are the main causes for flooding. Based on data, in 2021 there were 2 floods that resulted in damage to infrastructure, housing, and public facilities, which caused substantial material losses beside the immaterial things. There were 785 families that were impacted by floods in 2021. Therefore, Students of Mataram University (UNRAM) who were conducting Integrated Community Service in the area Period of 2021/2022 carry out programs, one of which was called "Towards a Disaster Response Village" (DESTANA). This service is an effort to prevent the danger of flooding and land sliding in the future by planting a thousand trees at certain points agreed by the leadership, village officials, and the local community. The seedlings planted were provided by the Mataram Environment and Forestry Service, West Nusa Tenggara. This service is carried out in mutual cooperation with Bhabinsa, village officials, and the local community. Activities were conducted in stages in several designated hamlets. The community were very enthusiastic about the program with the hope that this program will be able to reduce or even overcome floodings and landslides. Moreover, we all hope that the Tree Planting Program increases the awareness of the communitiy about protecting the environment. We do hope that the trees will reduce the increase of global warming.
Mengenal Fitoplankton untuk Penguatan Materi Kompetensi Dasar Protista Pada Siswa Jurusan IPA (Biologi) MA Hidayatul Muhsinin Desa Labulia Lombok Tengah Lalu Japa; Syamsul Bahri; Prapti Sedijani
Jurnal Pengabdian Magister Pendidikan IPA Vol 1 No 1 (2018)
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.019 KB) | DOI: 10.29303/jpmpi.v1i1.231

Abstract

Abstrak: Berdasarkan kajian nilai ujian nasional Sekolah Mengengah Atas (SMA) dan Madrasah Aliyah (MA) tiga tahun berturut-turut (2008-2010) untuk kompetensi dasar (KD) yang nilainya selalu rendah adalah KD Protista. Dari hasil penelitian tahun 2010, terungkap bahwa kegiatan parktikum (termasuk pengamatan Protista) tidak bisa dlaksanakan secara maksimal karena berbagai kendala seperti mulai dari tidak cukup tersedia sarana pendukung, tidak cukup waktu, dan tidak tahu bagaimana melakukan kegiatan tersebut. Karena itu diperlukan kegiatan khusus pengenalan fitoplankton sebagai salah satu upaya penguatan materi Protista yang menyangkut: pengenalan spesies yang umum dijumpai, sumber sampel, dan teknik pengamatan dengan mikroskop. Kegiatan pengabdian ini ditujukan untuk penguatan atau pengayaan pengetahuan dan keterampilan siswa MA Hidayatul Muhsinin Desa Labulia Lombok Tengah tentang fitoplankton sebagai komponen utama materi KD Protista. Kegiatan pengabdian ini akan dilakukan dalam bentuk penyampaian teori dan praktek pengamatan secara langsung menggunakan mikroskop. Siswa kelas III jurusan IPA (Biologi) MA Hidayatul Muhsinin, peserta kegiatan ini sangat bersemangat melakukan pengamatan di bawah mikroskop yang sebelumnya belum pernah mereka lakukan. Mereka baru sadar bahwa dalam satu tetes air terdapat banyak sekali individu sel berbagai spesies fitoplankton yang bisa disaksikan. Pembelajaran teoritis di kelas diperkuat dengan fakta yang diamati dengan mikroskop. Kata kunci: IPA(Biologi), Fitoplankton, Madrasah Aliyah, Penguatan, Protista, Siswa
Penyuluhan Tentang Usaha Perbaikan Gizi Keluarga (UPGK) Melalui Pemanfaatan Pekarangan dimasa Pandemi Covid 19 Kusmiyati Kusmiyati; Dewa Ayu Citra Rasmi; Khairuddin Khairuddin; Prapti Sedijani; Baiq Sri Handayani
Jurnal Pengabdian Magister Pendidikan IPA Vol 4 No 3 (2021)
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (89.798 KB) | DOI: 10.29303/jpmpi.v4i3.967

Abstract

Pandemi Covid 19 sudah berlangsung dari bulan Maret 2020, sejak itu berbagai aktivitas yang dilakukan masyarakat biasanya terkait dengan menjaga agar imunitas tubuh tetap terjaga. Menjaga imunitas tubuh tidak terlepas dari pemenuhan gizi sehari-hari. Usaha Perbaikan Gizi Keluarga (UPGK) merupakan sebuah usaha untuk melakukan kegiatan terpadu, bertujuan untuk menanggulangi kekurangan gizi atau untuk memperbaiki gizi masyarakat. Tujuan dari pengabdian ini adalah mengajak mahasiswa agar mau memanfaatkan pekarangan untuk perbaikan gizi keluarga. Kegiatan pengabdian ini dilaksanakan dengan beberapa tahap, yaitu tahap persiapan, tahap observasi, tahap pelaksanaan kegiatan dan tahap pelaporan. Metode yang digunakan meliputi: (a) Metode ceramah, digunakan untuk menyampaikan materi usaha perbaikan gizi keluarga melalui  pemanfaatan pekarangan di masa pandemi covid 19. (b). Metode tanya jawab, digunakan untuk memberikan umpan balik pada peserta sekaligus untuk mendapatkan tanggapan peserta tentang materi yang telah disampaikan selama kegiatan. Selain itu, untuk evaluasi kegiatan pengabdian ini dilakukan pretes dan postes. Hasil pengabdian menunjukkan bahwa antusias mahasiswa pada saat pelaksanaan kegiatan sangat tinggi, terlihat mahasiswa mengikuti dari awal hingga akhir, pada waktu dilakukan pretes dan postes, mahasiswa mengirim kembali jawaban pertanyaan melalui google form. Selanjutnya ketika penyampaian  materi, mahasiswa juga dapat diajak interaksi, dengan mengajukan pertanyaan dari materi yang disampaikan. Hasil penghitungan gain ternormalisasi sebesar 0,53. Kesimpulan dari kegiatan pengabdian ini adalah: a). Pengetahuan mahasiswa tentang UPGK dan pemanfaatan pekarangan meningkat, dalam kategori sedang; b). Pemanfaatan pekarangan perlu ditata dengan mengedepankan keberagaman spesies untuk kebutuhan dapur, sehingga dapat memenuhi kebutuhan gizi dan memperbaiki gizi keluarga  di masa pandemi covid 19.