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All Journal International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry
Mieke Hemiawati Satari
Faculty of Dentistry, Universitas Padjadjaran
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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The effect of ethanol extract of mangostene (Garcinia Mangostana Linn.) peel on tongue cancer cells Supris Clone-1 apoptosis, in vitro Satari, Mieke Hemiawati; Titiana, Maria Aurora; Oewen, Roosje Rosita
Padjadjaran Journal of Dentistry Vol 23, No 3 (2011): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1045.519 KB) | DOI: 10.24198/pjd.vol23no3.14029

Abstract

Apoptosis or programmed cell death serves to regulate physiological and pathological conditions. Garcinia mangostana Linn. is one of the medical herbs that is widely used to suppress human tongue cancer growth by inducing apoptosis. The research aimed to analyze the effect of ethanol extract of Garcinia mangostana Linn. on tongue cancer cells apoptosis Supris Clone-1 (SP-C1). The type of the research was an experimental laboratory. Tongue cancer cells SP-C1 were treated by various ethanol extract concentrations (0, 300, 400, 500, 600, 700 pg/ml) of Garcinia mangostana Linn. to detect the apoptosis, which was done by acridine orange and ethidium bromide coloring tests. The number of tongue cancer cells SP-C1 2x104 cells/dish. Observation on cells apoptosis was observed by a fluorescent microscope with 40 x magnification. The data were analyzed using one-way ANOVA and was followed by Post Hoc test (Tukey-test) with 95% significance level. The result showed that tongue cancer cells SP-C1 apoptosis treated by various ethanol extracts of Garcinia mangostana Linn. increase significantly. The highest effect of apoptosis was detected at the 700 pg/ml concentration that has an effect on the percentage of tongue cancer cells apoptosis by 65%.
Anticancer potency of black sea cucumber (Holothuria atra) from Mentawai Islands, Indonesia Satari, Mieke Hemiawati; Arma, Utmi; Ilyas, Syafruddin; Handayani, Dian
Padjadjaran Journal of Dentistry Vol 29, No 1 (2017): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.217 KB) | DOI: 10.24198/pjd.vol29no1.11654

Abstract

Introduction: The source of bioactive compounds believed to have strong anticancer potency is derived from sea cucumber. Black sea cucumber (Holothuria atra) is a dominant species in Mentawai Islands, West Sumatera, Indonesia. Key factor compound that acts as anticancer in sea cucumber extract is tritepenoid also known as Frondoside A. The purpose of this study was to determine the effectiveness of the active compound taken from black sea cucumber as anticancer. Methods: Methods used was experimental laboratory. Anticancer activity of black sea cucumber was determined by using the MTT assay cytotoxic test to measure the IC50. Cytotoxic test was conducted with Supris clone-1 (SP-C1) derived from cloned lymphadenopathy of patients with oral squamous cell carcinoma on lingual part. Results: It was found that the compounds contained in black sea cucumber has strong anticancer activity (IC50 = 5.25 μL/mL) against SP-C1. Conclusion: Black sea cucumber extract has strong cytotoxic effects against cancer cells SP-C1, therefore black sea cucumber extract potentially developed as a new source of cancer medicine.
Anti-tumor agent celecoxib activity towards SP-C1 tongue cancer cells invasion (in vitro) Achmad, Harun; Satari, Mieke Hemiawati; Oewen, Roosje Rosita; Supriatno, S.
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1465.155 KB) | DOI: 10.24198/pjd.vol23no1.14053

Abstract

Invasion is a characteristic of the occurrence of cancer and indicates the cancer cells capability to destroy and degrade the border between the epithet and basal membrane to further spread into the surrounding extra-cellular matrix. The purpose of this research was to find the existence of impediment at the SP-C1 tongue cancer cell using celecoxib chemopreventive medication. The SP-C1 tongue cancer cells were treated in vitro using celecoxib medication as a research subject at the following concentrations 5, 10, 25, 50, 75, 100, 125%; and 0 as control group (only DMEM growth medium treatment). Pure experimental testing was carried out for 24 and 48 hours, with observation and calculation of an average number of SP-C1 tongue cancer cells. The data collected were analyzed using the ANOVA test with Newman Keuls paired range test or t-test. Research results indicated that the average number of SP-C1 tongue cancer cells invasion after administration of celecoxib medication based on administration concentration and time statistically yielded significant results. The ANOVA test results were statistically significant, that is, average occurrence of the number of SP-C1 tongue cancer cells due to the use of celecoxib at certain concentrations compared to that without celecoxib was different. At celecoxib of zero (control) concentration was 24.4 with celecoxib concentration starting at 5 up to 125% experienced a decline from its average 11 to become 2.3. The conclusion of the research was that the greater the celecoxib concentration administered, the greater the effect on the impediment of SP-C1 tongue cancer cell invasion.
Role of Streptococcus Anginosus on the formation of dental caries Nonong, Yetty Herdiyati; Satari, Mieke Hemiawati; Sasmita, Inne Suherna
Padjadjaran Journal of Dentistry Vol 23, No 3 (2011): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1890.805 KB) | DOI: 10.24198/pjd.vol23no3.14031

Abstract

Generally, the etiology of dental caries is the cariogenic properties of bacteria, these are always associated with Streptococcus mutans. Glucosyltransferase fragment (Gtf) are also in other strains of Streptococcus such as Streptococcus anginosus, Streptococcus milleri which includes beta hemolysis. Genotypically B Streptococcus anginosus has genetic characteristics that are similar to Streptococcus mutans. The research objective was to determine the existence of Gtf B/C gene as a cause of caries in Streptococcus anginosus. The study was conducted in experimental laboratories with PCR technique by taking a sample of 20 children who had caries. The results showed there was the amplification of Streptococcus anginosus with a level of homology 96%, 97%, and 99%. The results of the Gtf genes amplification fragment B/C provided 600 pb ribbon. The conclusion was Streptococcus anginosus classified as cariogenic bacteria because they had Gtf B/C genes.
Antibacterial effect of pineapple (Ananas comosus) extract towards Staphylococcus aureus Loon, Yong Ker; Satari, Mieke Hemiawati; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 30, No 1 (2018): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.911 KB) | DOI: 10.24198/pjd.vol30no1.16099

Abstract

Introduction: Staphylococcus aureus is one of the important medical pathogens which have been recognised for many years as a remedyfor a wide case of oral infections. Nowadays, the use of herbal remedy for reducing bacteria in the oral cavity has been implemented widely due to thefewer side effects. Therefore, researchershave been findingwaysto use pineapple in dentistry to prevent many cases of oral diseases. The purpose of this study was to prove that pineapple extract indifferent concentration had the potential as an antibacterial agent towards Staphylococcus aureus. Methods: The study was an experimental laboratory conducted by determining the minimum inhibitory concentration of pineapple (Ananas comosus) with thetwo-fold serial dilution methods. Results:The The Minimum Inhibitory Concentration (MIC) of the pineapple extract was 1.56%-0.78%. Conclusion: The pineapple extract had anantibacterial effect towards Staphylococcus aureus due to the bromelain compound and its phytochemical factor such as Vitamin C and flavonoid. 
Antibacterial effect of clove (Eugenia aromaticum) oil extracted from clove cigarettes towards Streptococcus mutans bin Jumali, Ahmad Alif Wazir; Satari, Mieke Hemiawati; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 25, No 1 (2013): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.426 KB) | DOI: 10.24198/pjd.vol25no1.15428

Abstract

Streptococcus mutans is a commensal bacteria of the oral cavity, particularly found in dental plaque attached to the tooth surface, and can also found in the saliva, buccal mucosa, tongue, and the gingival sulcus. Clove cigarette contains the clove oil used worldwide as a herbal remedy for a variety of health disorders due to its antibacterial, antifungal, and antiviral properties. The purpose of this study was to determined the antibacterial effect of the clove oil contained in the clove cigarette towards Streptococcus mutans as a cariogenic bacteria. The research was an experimental laboratory, which tested the sensitivity of the Streptococcus mutans bacteria taken from the saliva of 10 clove cigarette smokers towards the clove oil extract of the clove cigarette in-vitro by using the Kirby-Bauer method. The study showed that the clove oil extract of the clove cigarette which contained eugenol has antibacterial towards the growth of Streptococcus mutans, and there was a difference of the antibacterial activity between clove oil extracted from minced and combusted clove cigarette. The conclusion of this study was the clove oil extracted from minced clove cigarette had a better antibacterial effect than the combusted clove cigarette.
Bacterial inhibition test of methanolic extracts of strawberry (Fragraia x ananassa Duchesne), lime (Citrus aurantifolia), and radish (Raphanus sativus L.), towards Streptococcus Sanguis ATCC 10556 Anggraini, Fitri; Satari, Mieke Hemiawati; Mariam, Marry Siti
Padjadjaran Journal of Dentistry Vol 30, No 2 (2018): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.37 KB) | DOI: 10.24198/pjd.vol30no2.18325

Abstract

Introduction: Caries are initiated by the plaque formation on the tooth surface, due to the interaction between food debris and bacteria in the mouth. The pioneer bacterium of plaque formation is Streptococcus sanguis. The  Strawberry fruit, lime fruit, and radish root are part of the plant that contains antibacterial substances. Flavonoid, tannin, saponin, alkaloid, polyphenol, terpenoid and quinon as antibacterial substances. This study aimed to evaluate strawberry fruit, lime fruit, and radish root methanol extract can inhibit Streptococcus sanguis ATCC 10556. Therefore, strawberry, lime, and radish can be made into mouthwash, bubble gum, or toothpaste to prevent plaque formation. Methods: This study used a Kirby-Bauer diffusion test as the inhibition test. Results: The result of this study that the biggest inhibition zone was lime methanol extract 20,000 ppm, and the smallest one was radish methanol extract 5,000 ppm.  Conclusion: Strawberry fruit, lime fruit peel, and radish root methanol extract and their combination had the effect of inhibiting bacterial growth synergistically against Streptococcus sanguis ATCC 10556.
Perbedaan jumlah koloni Streptococcus mutans pada perokok kretek dan bukan perokokDifference between the amount of Streptococcus mutans colonies in kretek smokers and non-smokers Melani, Intan; Satari, Mieke Hemiawati; Malinda, Yuti
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 30, No 2 (2018): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.003 KB) | DOI: 10.24198/jkg.v30i3.18510

Abstract

Pendahuluan: Rokok kretek merupakan jenis rokok yang paling populer di Indonesia. Merokok dapat meyebabkan berbagai gangguan dalam rongga mulut, salah satunya yaitu karies. Streptococcus mutans merupakan salah satu bakteri yang berperan penting dalam proses terjadinya karies. Penelitian ini bertujuan untuk mengetahui perbedaan jumlah koloni Streptococcus mutans pada perokok kretek dan bukan perokok. Metode: Penelitian deskriptif ini dilakukan dengan teknik pengambilan sampel merujuk pada penelitian yang dilakukan Buduneli N, dkk. jumlah sampel yang diperoleh 20 orang yang terdiri dari 10 perokok kretek dan 10 bukan perokok. Bahan pemeriksaan berupa saliva diambil dari masing-masing sampel. Sampel saliva diencerkan, dieramkan dan diinokulasi pada media TYCSB. Sampel saliva kemudian diinkubasi pada 37 0C, secara fakultatif anaerob selama 48 jam. Koloni yang tumbuh dihitung menggunakan colony counter. Data dianalisis menggunakan t-test independen (α=0,05). Hasil: Rata-rata jumlah koloni Streptococcus mutans dari saliva perokok kretek sebesar 47,65 x 102 CFUs/ml sedangkan pada bukan perokok sebesar 11,1 x 102 CFUs/ml. Data statistik uji t independen mendapatkan nilai p<0,05 yang berarti terdapat perbedaan yang signifikan antara jumlah koloni Streptococcus mutans pada perokok kretek dan bukan perokok. Simpulan: Jumlah koloni Streptococcus mutans pada perokok kretek lebih tinggi dibandingkan bukan perokok. ABSTRACT Introduction: Kretek cigarettes are the most popular type of cigarette in Indonesia. Smoking can cause various disorders in the oral cavity, one of them is caries. Streptococcus mutans is one of the bacteria that play an important role in the process of caries. This study was aimed to determine the difference between the amount of Streptococcus mutans colonies in kretek smokers and non-smokers. Methods: A descriptive study was carried out with the sampling technique referring to the research conducted by Buduneli N et al. The amount of samples obtained were as much as 20 people consisted of ten kretek smokers and ten non-smokers. Examination material in the form of saliva was taken from each sample. Salivary samples were diluted, scooped and inoculated on TYCSB media. Salivary samples were then incubated at 37°C, facultatively anaerobically for 48 hours. Growing colonies were calculated using a colony counter. Data obtained were analysed using independent t-test (α = 0.05). Results: The average amount of Streptococcus mutans colonies from kretek smoker saliva was 47.65 x 102 CFUs / ml while in the non-smokers was 11.1 x 102 CFUs / ml. Statistical data of the independent t-test obtained the p-value < 0.05, which means that there was a significant difference between the amount of Streptococcus mutans colonies in kretek smokers and non-smokers. Conclusion: The amount of Streptococcus mutans colonies in kretek smokers saliva is higher than the non-smokers. Keywords: Kretek cigarettes, non-smokers, Kretek cigarette smokers, Streptococcus mutans.
Daya hambat ekstrak metanol nanas, belimbing wuluh, dan kemangi terhadap Streptococcus mutans ATCC 25175Inhibitory potential of methanolic extract of pineapple, wuluh starfruit, and basil towards Streptococcus mutans ATCC 25175 Budiani, Yona Ayumi; Satari, Mieke Hemiawati; Jasrin, Tadeus Arufan
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 29, No 2 (2017): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (629.548 KB) | DOI: 10.24198/jkg.v29i2.18575

Abstract

Pendahuluan: Karies merupakan suatu penyakit infeksi dimana terjadi proses demineralisasi progresif pada jaringan keras gigi. Proses demineralisasi gigi dipengaruhi oleh pH asam pada mulut yang disebabkan oleh Streptococcus mutans. Tujuan peneltian adalah untuk menguji daya hambat dari ekstrak metanol nanas, belimbing wuluh, dan kemangi terhadap Streptococcus mutans sebagai bakteri yang berperan dalam proses pembentukan plak serta karies gigi. Metode: Penelitian dilakukan secara eksperimental dengan cara meneteskan ekstrak metanol nanas, belimbing wuluh, dan kemangi, serta ekstrak kombinasi ketiganya yang telah diencerkan dengan pelarut Dimetil Sulfoksida (DMSO) ke dalam lubang sumuran pada lempeng agar Mueller Hinton yang telah ditanami Streptococcus mutans. Bakteri uji berupa Streptococcus mutans ATCC 25175 yang diperiksa secara mikroskopis, ditanam pada lempeng agar Muller Hinton dan inkubasi dalam suasana fakultatif anaerob pada suhu 37OC selama 24 jam. Konsentrasi ekstrak yang diuji adalah 20.000 ppm, 10.000 ppm. 5.000 ppm, dan 1.000 ppm. Hasil: Hasil penelitian menunjukan bahwa ekstrak metanol nanas, belimbing wuluh, dan kemangi memiliki daya hambat terhadap Streptococcus mutans ATCC 25175. Simpulan: Daya hambat yang terjadi pada bakteri disebabkan oleh kandungan zat aktif yang dimiliki nanas, belimbing wuluh, dan kemangi seperti enzim bromelain, flavonoid, tanin, alkaloid, triterpenoid, dan saponin. ABSTRACTIntroduction: Caries is an infectious disease in which there is a progressive demineralization process in hard teeth. The demineralization process of teeth is affected by acid pH in the mouth caused by Streptococcus mutans. The aim of the study was to examine the inhibitory potential of methanol extract of pineapple, starfruit and basil to Streptococcus mutans as bacteria that play a role in the process of plaque formation and dental caries. Methods: The study was carried out experimentally by dripping the methanol extract of pineapple, starfruit and basil, and the combination of the three extracts which had been diluted with Dimethyl Sulfoxide (DMSO) solvent into the well hole on the agar plate of Mueller Hinton which had been planted with Streptococcus mutans. Test bacteria in the form of ATCC 25175 Streptococcus mutans were examined microscopically, planted on Muller Hinton agar plate and incubated in anaerobic facultative atmosphere at 37OC for 24 hours. The concentration of extract tested was 20,000 ppm, 10,000 ppm. 5,000 ppm, and 1,000 ppm. Result: The results showed that the methanol extract of pineapple, starfruit and basil had inhibitory potential against Streptococcus mutans ATCC 25175. Conclusion: The inhibitory potential that occurs in bacteria is caused by the active substance possessed by pineapple, starfruit, and basil such as bromelain enzyme, flavonoids, tannins, alkaloids, triterpenoids, and saponins.Keywords: Streptococcus mutans, pineapple, Ananas comosus, starfruit, Averrhoa bilimbi, basil, Ocimum tenuiflorum, inhibitory test.
Perbedaan potensi antibakteri ekstrak metanol umbi sarang semut (Myrmecodia pendens Merr. & Perry) dan NaOCl terhadap Streptococcus mutans (ATCC 25175)The antibacterial potential differences between the methanolic extract of ant-plant (Myrmecodia pendens Merr. & Perry) tubers and NaOCl towards Streptococcus mutans (ATCC 25175) Apriyanti, Eria Ariningtyas; Satari, Mieke Hemiawati; Laksono, Bremmy
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 28, No 2 (2016): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.406 KB) | DOI: 10.24198/jkg.v28i2.18704

Abstract

Pendahuluan: Streptococcus mutans merupakan organisme kariogenik utama. Antibakteri sintetik yang biasa digunakan adalah NaOCl. Sarang semut mengandung senyawa kimia yang dapat menghambat pertumbuhan Streptococcus mutans. Tujuan penelitian ini adalah mengetahui daya hambat ekstrak metanol sarang semut (Myrmecodia pendans Merr. & Perry) dan NaOCl terhadap Streptococcus mutans serta perbedaan potensi antibakteri keduanya. Metode: Jenis penelitian adalah eksperimental laboratoris. Uji daya antibakteri dilakukan dengan menggunakan metode difusi agar. Media agar darah ditetesi 0,1 ml suspensi bakteri lalu dimasukkan ekstrak metanol sarang semut dengan konsentrasi 30%, 15%, 7,5%, 3,75%, 1,875% dan NaOCl dengan konsentrasi 5%, 2,5%, 1,25% diinkubasi pada suhu 37° C selama 24 jam dalam suasana fakultatif anaerob dan dilakukan replikasi tiga kali. Uji statistik menggunakan metode ANAVA dan Independent t-test. Hasil: Ekstrak metanol sarang semut konsentrasi 30% menghasilkan diameter zona hambat rata-rata 5,87 mm, sedangkan NaOCl 1,25% adalah 9,33 mm. Simpulan: Ekstrak metanol sarang semut dan NaOCl memiliki potensi daya hambat terhadap Streptococcus mutans, namun potensi antibakteri NaOCl lebih besar dibandingkan dengan ekstrak metanol sarang semut. ABSTRACTIntroduction: Streptococcus mutans is the main cariogenic organism. The synthetic antibacterial commonly used is NaOCl. Ant nests contain chemical compounds that can inhibit the growth of Streptococcus mutans. The purpose of this study was to determine the inhibitory power of methanol extracts of ant nests (Myrmecodia pendans Merr. & Perry) and NaOCl against Streptococcus mutans and the differences in antibacterial potential both. MethodS: This type of research is experimental laboratory. Antibacterial power test was carried out using agar diffusion method. Blood agar media was dripped with 0.1 ml of bacterial suspension and then inserted methanol extract of ant nests with a concentration of 30%, 15%, 7.5%, 3.75%, 1.875% and NaOCl with a concentration of 5%, 2.5%, 1, 25% was incubated at 37C for 24 hours in a facultative anaerobic atmosphere and replicated three times. Statistical test using ANAVA method and Independent t-test. Results: Methanol extract of ant nest 30% resulted in an average inhibition zone diameter of 5.87 mm, while 1.25% NaOCl was 9.33 mm. Conclusion: Methanol extract of ant nests and NaOCl has potential inhibitory power against Streptococcus mutans, but the antibacterial potency of NaOCl is greater than that of methanol extracts of ant-plants.Keywords: Antibacterial, methanolic extract, ant-plants, NaOCl, Streptococcus mutans.
Co-Authors Apriyanti, Eria Ariningtyas Balafif, Felisha Febriane bin Jumali, Ahmad Alif Wazir Bremmy Laksono Budiani, Yona Ayumi Diah Dhianawaty Dian Handayani Edeh Rolleta Firman Fuad Wirakusumah Fitri Anggraini, Fitri Harun Achmad, Harun Inne Suherna Sasmita Jasrin, Tadeus Arufan Johanes C. Mose Leri Septiani Loon, Yong Ker Made Kornia Karkata Maria Aurora Titiana, Maria Aurora Marry Siti Mariam Melani, Intan Mintareja Teguh Moch . Rodian Roosje Rosita Oewen, Roosje Rosita S. Supriatno, S. Syafruddin Ilyas Utmi Arma, Utmi Warta Dewi, Warta Yetty Herdiyati Nonong, Yetty Herdiyati Yuti Malinda, Yuti