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All Journal JURNAL GIZI INDONESIA Medical Journal of Indonesia Indonesian Journal of Agricultural Science AGRIVITA, Journal of Agricultural Science
Satoto Satoto
Balai Besar Penelitian Tanaman Padi Jl. Raya 9 Sukamandi Subang 41256, Jawa Barat
Agriculture, Biological Sciences & Forestry Health Professions Medicine & Pharmacology

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THE SEGREGATION PATTERN OF INSECT RESISTANCE GENES IN THE PROGENIES AND CROSSES OF TRANSGENIC ROJOLELE RICE Satoto, Satoto; Sulistyowati, Yuli; Hartana, Alex; -Loedin, Inez H. Slamet
Indonesian Journal of Agricultural Science Vol 9, No 2 (2008): October 2008
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Successful application of genetic transformation technique, especially in developing rice variety resistant to brown plant hopper and stem borer, will depend on transgene being expressed and the gene inherited in a stable and predictable manner. This study aimed to analyse transgene segregation pattern of the progenies and the crosses of transgenic rice cv. Rojolele harboring cry1Ab and gna genes. The third generation (T2) of fivetransgenic Rojolele events containing gna and/or cry1Ab were evaluated for two generations to identify the homozygous lines and to study their inheritance. The homozygous lines were selected based on the result of PCR technique. The segregation patterns of gna and cry1Ab were studied in eight F2 populations derived from Rojolele x transgenic Rojolele homozygous for cry1Ab and or gna and their reciprocal crosses. Data  resulted from PCR of F2 population were analysed using a Chi Square test.The study obtained six homozygous lines for gna, namely A22- 1-32, A22-1-37, C72-1-9, F11-1-48, K21-1-39, K21-1-48, and two homozygous lines for cry1Ab, namely K21-1-39 and K21- 1-48. Both cry1Ab and gna transgenes had been inherited through selfing and crossing with their wild type as indicated from the F1 containing gna and cry1Ab as many as 48.4% and 47.4%, respectively. In six of the eight crosses, gna was inherited in a 3:1 ratio consistent with Mendelian inheritance of a single dominant locus, while in the remaining two crosses, gna was segregated in a 1:1 ratio. The presence of cry1Ab in F2 populations also showed a 3:1 segregation ratio in all crosses. In the F2 population derived from F1 plant containing cry1Ab and gna, both transgenes segregated in a 9:3:3:1 dihybrid segregation ratio. This study will add to the diversity of geneticsources for insect resistance and allow further use of these transgenic lines for pyramiding resistance to brown plant hopper and stem borer or  separately in rice breeding programs whenever the efficacy tests and biosafety requirements have been completed.
Dietary plant food and socioeconomic determinants of vitamin A status : study in rural lactating woman during crisis in Central Java Sulchan, Mohammad; Satoto, Satoto
Medical Journal of Indonesia Vol 15, No 4 (2006): October-December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.019 KB) | DOI: 10.13181/mji.v15i4.247

Abstract

For the Longer term food-based approaches for controlling vitamin A deficiency and its consequences, become increasingly important. A nutrition survailance system in Central-Java, Indonesia assessed vitamin A intake and serum retinol concentration of lactating women with a child ≤36 mo old during crisis. Median vitamin A intake was 319 RE/d and night blindness 0,34%. Serum retinol concentration (mean : 1,23 µmol/L) was related to vitamin A intake in a dose-concentration manner. The multiple logistic regression model for predicting the chance for a serum retinol concentration > observed median of the population (≥1,27 µ mol/L) included determinant factors, vitamin A intake from plant foods (OR [95% CI] per quartile, 1st : 1.00, 2nd: 1,63 [0,99-2,80], 3rd: 1,99 [1,58-2,99], and 4th: 2,62 [1,68-4,04], from, animal foods (1st and 2nd: 1,00. 3rd: 1,37 [0,89-2,09] and 4th: 2,86 [1,59-3,98] ). Homegardening (no 1,00, yes 1,88 [1,08-2,68] ) and woman’s education level (≤ primary school : 1,00 ≥ secondary school : 1,46 [1,00-2,16] ). Thus, although contributing 16 times more to total vitamin A intake plant foods were as important for vitamin A status as animal foods. Homegardening and woman’s education level seem to reflect longer-term consumption of plant and animal foods respectively. (Med J Indones 2006; 15:259-66)Keywords: Vitamin A intake, plant foods, animal foods, vitamin A status, homegardening, socio-economic status, crisis, Indonesia
YIELD STABILITY OF NEW HYBRID RICE ACROSS LOCATIONS Satoto, Satoto; Rumanti, Indrastuti Apri; Widyastuti, Yuni
AGRIVITA, Journal of Agricultural Science Vol 38, No 1 (2016): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i1.675

Abstract

The adaptation of hybrid rice varieties mostly are in specific location and season, but there are some of the varieties have a wide adaptation then adopted by the farmer in the large area. Replicated yield trials were conducted to study the stability of hybrid rice yield and identify the best location to optimize their yield per ha. We have conducted the trials in three location such as Sukamandi, Salatiga and Malang during two seasons in 2011. We are analyzing data across location and season  using AMMI and Eberhart Russel methods. The AMMI analysis showed that the  IR79156A/PK88 was adaptable to favorable environments but unstable. This hybrid is always performing well and produce the higher yield compare to check variety. Some of other hybrids  were good only in specific location, i.e. IR62829A/BP2280-1E-12-22 and IR58029A/BP2 280-1E-12-22. Those hybrids produced higher yield in Salatiga and Malang, respectively. Seem to AMMI analysis, the result of Eberhart and Russells method also showed that IR79156A/PK81  was the best hybrid with regression slope (b) around 1 with the yield average higher than average of all hybrids. It indicated that this hybrid has a wide adaptation and probably can be cultivated in the wider ecosystem.
SCHOOL FEEDING PROGRAM USING RICE-PEA BEVERAGE IN SOME PRIMARY SCHOOLS: DOES IT HAVE BENEFIT? Satoto, Satoto; Rahfiluddin, Z; Sakti, H
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Volume 1. Nomor 1. Desember 2005
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jgi.1.1.

Abstract

ABSTRACT School feeding program is believed to be one effort among others to improve attendance rate, nutritional status and cognitive function of school children, which in turn improve academic performance of them. A study was conducted to evaluate a school feeding program using rice-pea beverage in Bandung, Indonesia. Its objective is to measure effect of the program on attendance rate, nutritional status, cognitive function and academic performance of the children joining the program. A control group design was administered. A number of students of class 3,4 and 5 from schools joining the feeding program were selected as program group, and more or less same number of school children in other schools with similar socio-economic situation were selected as control group. The feeding program was organized for 6 months. Attendance rate was measured by number of absentees, total and due to sickness in one semester. Nutritional status was standardized using WHO-NCHS z-score for weight-for-age (WAZ) and height-for-age (HAZ). Cognitive function was measured by Coloured Progressive Matrices (CPM), and score of Mathematics and Indonesian Language (Bahasa Indonesia) were measured using specific scholastic testings. School and home learning environment, certain food consumption frequencies were collected as covariates. GLM analyses were administered. The study found that after being controlled by some covariates, at the end of the study children in the program group showed better attendance rate, nutritional status, cognitive function and school performance. It is assumed that the improvement was due to better attendance rate as the program attracted the children to attend more days in the schools and due to improvement of food consumption provided by the program and at home. More in-depth, more specific and longer study, administering randomized case-control trial is recommended. Comparison of the use of foodstuffs for the purpose is also interesting to be studied. Permalink : http://ejournal.undip.ac.id/index.php/jgi/article/view/3239
Co-Authors ALEX HARTANA H Sakti Indrastuti Apri Rumanti Inez H. Slamet -Loedin Mohammad Sulchan Yuli Sulistyowati Yuni Widyastuti Z Rahfiluddin