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Pengaruh Likopen terhadap Penurunan Aktivitas Mitogen-Activated Protein Kinase (MAPK) dan Ekspresi Endothelin-1 (ET-1) pada Kultur Huvecs yang Dipapar Leptin Fatmawati, Heni; Satuman, Satuman; Rudijanto, Ahmad; Indra, Muhammad Rasjad
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.081 KB) | DOI: 10.31258/jnat.13.2.162-167

Abstract

The effect of obesity on vascular function is mediated by hormon leptin. Leptin has been proved to increaseoxidative stress in endothelial cell. The previous study has proven that leptin caused the endothelial dysfunction asa step of the atherogenesis. Lycopene, an antioxidant, is presumed having the ability to block the atherogenesismechanism, which is stimulated a proinflamatory cytokine and adhesion molecules by MAPK and transcriptionfactor ET-1. Therefore, the aim of this research was to prove and to determine whether lycopene could decreasethe MAPK and ET-1 expression in Human Umbillical Vein Endothelial Cells (HUVECs) culture induced by 500 ng/mlleptin. In vitro study used primary culture of the HUVECs were devided in to 7 groups, there were (1) 0 ng/ml leptinand 0 ìM lycopene, (2) induced by 500 ng/ml leptin for 12 hours, (3) induced by leptin and lycopene with concentration10; 25; 40; 55 and 75 ìM for 12 hours. Then the identification of MAPK was applied by using imunocytochemistrycompared with ELISA procedure on cell endothel culture lysate and ET-1 expression was measured by using RTPCR. It was showed that lycopene 10-25 ìM decreased MAPK and ET-1 expression significantly in HUVECs cultureinduced by leptin 500 ng/ml. Leptin was increased ERK1/2 MAPK and ET-1 expression in HUVECs culture and candecrease by lycopene. Optimum dose of lycopene is 10-25 ìM.
Optimizing Collagenase, Fetal Bovine Serum, and Insulin to Isolate, Proliferate, and Differentiate Rat Preadipocyte in vitro Indra, M Rasjad; Satuman, Satuman; Widodo, Edwin
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.653 KB) | DOI: 10.33476/jky.v16i3.244

Abstract

BackgroundThe aim of this study is to develop a method for optimal in vitro proliferation and differentiation of rat adipocyte. MethodsPreadipocyte were isolated from omentum of Rattus norvegicus Wistar using Collagenase type I dan II (Sigma). Cells were cultured in M199 culture containing either 0%, 8% or 10% Fetal Bovine Serum (FBS). Insulin were added into the media once the cells attached to the culture plate Proliferating and differentiated cells were counted and analysed by using Oil Red O dan Hematoxylen (HE) staining. Results This study demonstrated that adipocyte could be isolated using Collagenase type I but not type II. The addition of 10% FBSsignificantly increased the number of preadipocyte and differentiation of adipocyte more than those of 8% FBS and without FBS. The timing of FBS addition was best performed on day 8 using 10% FBS. Specific adipocyte staining using Oil Red O revealed thatthere were core lipids in mature adipocyte. ConclusionsCollagenase tipe I could be used to isolate preadipocyte cells. Supplementation of culture media with 8-10% FBS could enhance the in vitro proliferation of preadipocyte. Standard media M199 containing 10% FBS and insulin may provide an environment to differentiate preadipocyte specifically into adipocyte in vitro.
Peran Puerarin terhadap Aktivitas Intra dan Ekstraseluler pada Kultur Human Umbilical Vein Endothelial Cells (HUVECs) Pasca Induksi Leptin Djati, Mochamad Sasmito; Satuman, Satuman; Ratnawati, Retty; Widyarti, Sri; Aisyah, Erly Nur; Hasanah, Noer; Astuti, Eko Puji; Rochmawati, Ririn
The Journal of Experimental Life Science Vol 1, No 1 (2011)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1196.788 KB) | DOI: 10.21776/ub.jels.2011.001.01.05

Abstract

Beberapa penelitian terkini menyebutkan bahwa leptin merupakan salah satu penyebab disfungsi endotel yang merupakan salah satu penyebab aterogenesis. Antioksidan puerarin diduga memiliki kemampuan untuk mencegah mekanisme aterogenesis yang distimulasi oleh beberapa sitokin. Berdasarkan hal tersebut, maka tujuan penelitian ini adalah membuktikan dan mengetahui potensi puerarin untuk menghambat ekspresi dan aktivitas intra dan ekstraseluler VCAM-1, PPAR-γ, SOD dan H2O2, apoptosis dan nekrosis pada kultur Human Umbilical Vein Endothelial Cells (HUVECs) yang diinduksi 25 ng ml-1 leptin. Penelitian ini mempergunakan sel kultur primer HUVECs yang dibagi menjadi empat kelompok perlakuan, yaitu kelompok 0 ng ml-1 dan 0 μM puerarin, kelompok sel yang diinduksi 25 ng ml-1 leptin selama 12 jam, kelompok induksi puerarin 5, 25, 200 dan 525 μM puerarin selama enam jam tanpa leptin, kelompok induksi leptin dan puerarin dengan konsentrasi 5, 25, 200 dan 525 μM selama enam jam. Aktivitas VCAM-1 dan PPAR-γ diketahui dengan analisis imunositokimia, metode ELISA digunakan untuk analisis aktivitas SOD dan H2O2. Apoptosis dan nekrosis sel dianalisis setelah HUVECs diberi penanda BrdU selama 20 jam. Data dianalisis dengan analisis satu jalur (ANOVA) dan dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan bahwa induksi 25 ng ml-1 dapat meningkatkan ekspresi VCAM-1 (2,68 ± 0,15)% dibandingkan dengan perlakuan 0 ng ml-1 (0,54 ± 0,15)%. Perlakuan induksi puerarin 5, 25, 200, 525 μM memberikan dampak negatif terhadap ekspresi VCAM-1 meskipun pengaruh ini tidak signifikan. Puerarin dapat menekan apoptosis dan nekrosis sel, 525 μM puerarin secara efektif dapat menekan ekspresi PPAR-γ. Puerarin tidak memberikan dampak yang signifikan terhadap aktivitas ekstraseluler berdasarkan hasil analisis aktivitas SOD dan H2O2.Kata kunci: apoptosis, H2O2, HUVECs, nekrosis, leptin, puerarin, VCAM-1, PPAR-γ, SOD
STUDY OF SOME BIOCHEMICAL PARAMETERS IN YOUNG MEN AS EFFECTED BY RAMADAN FASTING Indra, M. Rasjad; Satuman, Satuman; Widodo, Edwin; E.H, Tinny; S.W, Endang; Sudiarto, Sudiarto; Soemardini, Soemardini
Jurnal Kedokteran YARSI Vol 15, No 1 (2007): JANUARI - APRIL 2007
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.479 KB) | DOI: 10.33476/jky.v15i1.999

Abstract

The effect of Ramadan fasting on some blood parameters i.e. serum glucose, urea, uric acid, lipids and proteins, were investigated on young men in Ar Rohmah Islamic dormitory. Nineteen normal and healthy students aging between 12-25 years, residing in the Islamic dormitory, voluntarily to participated in the study. Blood samples were obtained from the volunteers on the 1st and 26th day of Ramadan and analyzed for the aforementioned biochemical parameters. A non-significant effect of Ramadan fasting was observed on most of the parameters studied. However, serum urea, triglycerides, total cholesterol and LDL-cholesterol were reduced significantly (p 0.05) but remained within the physiological limits. Decrease in blood urea has been attributed to the effect of at least protein and triglycerides intake to increase lipolytic effect. The reduction in serum cholesterol and LDL is a beneficial effect of Ramadan fasting. The results of the study indicated thatRamadan fasting is quite safe for normal healthy adults.