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All Journal Jurnal Ilmu Ternak dan Veteriner BERITA BIOLOGI
Paul Selleck
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Infection of Parainfluenza type 3 (PI-3) as one of the causative agent of pneumonia in sheep and goats Sendow, Indrawati; Syafriati, Tatty; Wiedosari, Ening; Selleck, Paul
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 1 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.275 KB) | DOI: 10.14334/jitv.v7i1.276

Abstract

Serological survey was conducted to obtain the prevalence of Parainfluenza type 3 (PI-3) reactor as one of the causative agent of pneumonia in sheep and goats in abatoir at Jakarta and some small holder farms in Indonesia. Serological test using serum neutralization from 724 goat sera and 109 sheep sera indicated that only 1% of goats were serologically reactors and none of sheep sera had antibodies against PI-3 virus. Isolation of the virus from 56 bronchus and trachea swab and 345 lungs indicated that only one sampel from lung showed cythopathic effect (CPE) in Madin Darby Bovine Kidney (MDBK) cell lines identification of the virus using serum neutralization test indicated that the virus neutralized reference PI-3 antisera. The isolate came from one lung (7%) of 24 that showed histopathologically pneumonia intertitialis that usually caused by viral infection. Key words: Parainfluenza type 3 (PI-3), virus isolation, sheep, goat, pneumonia intertisialis
STATUS INFEKSI VIRUS INFLUENZA PADA BEBERAPA SPESIES HEWAN SEBELUM WABAH AVIAN INFLUENZA H5N1 PADA UNGGAS DI INDONESIA Sendow, Indrawati; Adj id, RM Abdul; Selleck, Paul
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.355 KB) | DOI: 10.14203/beritabiologi.v10i4.760

Abstract

After outbreak of Avian Influenza HPAI in chicken in mid 2003 in Indonesia, there was a question whether Avian Influenza HPAI was already presence in animals before the outbreak. A retrospective study was conducted to gain information on the presence of Influenza A virus infection in a range of animal species that could be infected by the virus. A total of 1529 animal sera, from 8 species from 12 different provinces which were stored at the Bbalitvet (Research Institute for Veterinary Science) Serum Bank were tested against matrix antigen of Influenza A using the Agar Gel Immunodiffusion (AGID) test. The results indicated that only 0.6% of animal tested which consisted of 4% of duck sera and 0.4% of pig sera were reacted in the AGID test with weak reaction. Those sera were then tested against Influenza A group viruses using an Enzyme Linked Immunosorbent Assay (ELISA), indicated that Influenza A viruses were not detected in either duck and pig positive sera. Those sera which were also tested by HI test against antigens of HI, H3 and H7, also indicated that none of those sera were reacted. In addition, 134 lung of pigs from an abattoir were collected for virus isolation. The viral isolation on chicken embryonated eggs resulted in 12 samples that contained viruses with agglutinated goose and chicken red blood cells. Identification of viruses isolated was done by agglutination test and ELISA. The results showed that none of those isolates were Influenza Type A virus. This study showed that influenza A virus group infection was not detected in animal species sampled before outbreak of AI H5N1 in 2003 in Indonesia.
Infection of Parainfluenza type 3 (PI-3) as one of the causative agent of pneumonia in sheep and goats Indrawati Sendow; Tatty Syafriati; Ening Wiedosari; Paul Selleck
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 1 (2002): MARCH 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.275 KB) | DOI: 10.14334/jitv.v7i1.276

Abstract

Serological survey was conducted to obtain the prevalence of Parainfluenza type 3 (PI-3) reactor as one of the causative agent of pneumonia in sheep and goats in abatoir at Jakarta and some small holder farms in Indonesia. Serological test using serum neutralization from 724 goat sera and 109 sheep sera indicated that only 1% of goats were serologically reactors and none of sheep sera had antibodies against PI-3 virus. Isolation of the virus from 56 bronchus and trachea swab and 345 lungs indicated that only one sampel from lung showed cythopathic effect (CPE) in Madin Darby Bovine Kidney (MDBK) cell lines identification of the virus using serum neutralization test indicated that the virus neutralized reference PI-3 antisera. The isolate came from one lung (7%) of 24 that showed histopathologically pneumonia intertitialis that usually caused by viral infection. Key words: Parainfluenza type 3 (PI-3), virus isolation, sheep, goat, pneumonia intertisialis
Co-Authors Adj id, RM Abdul Ening Wiedosari Ening Wiedosari Indrawati Sendow Indrawati Sendow Tatty Syafriati