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All Journal Natural Science: Journal of Science and Technology EUGENIA Jurnal Natur Indonesia Prosiding Seminar Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology
LANGKAH SEMBIRING
Laboratorium Mikrobiologi Fakultas Biologi Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Immunology & microbiology Materials Science & Nanotechnology

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Isolasi dan Karakterisasi Jamur Pendegradasi Katekin dari Seresah Pinus Nurnawati, Elisa; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 3 (2003): October 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.808 KB) | DOI: 10.24002/biota.v8i3.2855

Abstract

Isolation of catechin-degrading fungus from pine litter samples was done using minimal medium that containing catechin as sole carbon and energy source.  A total of 53 isolates were chosen to represent different colonial types of catechin degrading-fungus. The isolates were screened for their ability to degrade catechin in three stages. The first stage of screening was based on their ability to grow on solid medium containing 2 mM, and as a result, 28 isolates were selected.  The second stage of screening on the same medium but containing 4 mM of catechin resulting in 14 selected isolates. The third stage screening was based on their mean growth rate constant (k), instantaneous growth rate constant (m) and generation time (g) on minimal medium containing 4 mM catechin. The result showed that four isolates (D9, K2, K11, and S11) were the best catechin degradator. Further growth kinetic study  (k, m ,and g) of selected  isolates   indicated that  D9, K2, and S11 grew well on the medium containing 40 mM, but  K11 was inhibited by concentration of higher than 10 mM. Catechin biodegradation process was determined by following the decrease of catechin concentration on liquid medium. It was found that isolate K2 had higher ability to degrade catechin than the isolate K11. Finally, the four selected isolates from the third stage were characterized in terms of macroscopic, microscopic and phenotypic characters and identified. The result of the study showed that the isolates D9, K2 and S11 were identified as member of Aspergillus niger group. The isolate D9 was very similar to isolate S11, while the isolate K2 was found to be the most similar with Aspergillus niger van Tiegh. IFO 6341. The isolate K11 was assigned to be member of the genus Trichoderma.
ISOLASI, KARAKTERISASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT PENGHASIL Α & Β-GALAKTOSIDASE PRODUK FERMENTASI KULIT BUAH CEMPEDAK [ARTHOCARPUS INTEGER (THUNB.) MERR.] DAN BUNGA TIGARUN [CRATAEVA NURVALA BUCH-HAM] Lambui, Orryani; Sembiring, Langkah; Rahayu, S.
Natural Science: Journal of Science and Technology Vol 4, No 2 (2015): Volume 4 Number 2 (August 2015)
Publisher : Univ. Tadulako

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Abstract

Bakteri asam laktat (BAL) yang dapat menghasilkan ? & ?-galaktosidase telah diisolasi dari produk fermentasi kulit buah cempedak (mandai) dan bunga tigarun (jaruk) menggunakan medium MRS dengan metode enrichment dilanjutkan dengan surface spread plate dan dimurnikan dengan metode streak plate. Tahapan isolasi berhasil mengisolasi 73 isolat penghasil asam, kemudian di screening awal berdasarkan karakter kunci BAL. Diperoleh 26 isolat BAL dan dikarakterisasi berdasarkan kemampuannya dalam menghasilkan ? dan ?-galaktosidase melalui pengukuran aktivitas enzim dalam menghidrolisis subsrat p-nitrophenyl-?-D-galactopyronoside (pNPG) dan o-nitrophenyl-?-D-galactopyronoside (oNPG). Dua isolat terpilih yaitu OLM_10 memiliki nilai aktivitas ?-galaktosidase tertinggi yaitu 5,07 U/ml dan aktivitas ?-galaktosidase 0,43 U/ml dan OLJ_25 memiliki nilai aktivitas ?-galaktosidase 4,11 U/ml dan & aktivitas ?-galaktosidase tertinggi 1,06 U/ml. Berdasarkan karakterisasi terhadap 2 isolat terpilih didapatkan hasil bahwa isolat OLM_10 diidentifikasi sebagai anggota spesies Streptococcus thermophilus dan isolat OLJ_25 sebagai anggota spesies Lactobacillus plantarum.
APLIKASI METODE ARDRA DALAM IDENTIFIKASI ISOLAT Bacillus thuringiensis ENDOGENIK SEBAGAI PENGENDALI HAMA KUBIS (Crocidolomia binotalis) Salaki, Christina L.; Sembiring, Langkah
EUGENIA Vol 17, No 2 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.2.2011.3531

Abstract

ABSTRACT Indonesian indigenous bacterial isolates of B. thuringiensis pathogenic to cabbage pest (C. binotalis) were molecularly characterized and identified using DNA fingerprinting method of ARDRA (Amplified Ribosomal DNA Restriction Analysis). Chromosomal DNA of 10 selected isolates (SLK2.3, SRNG4.2, TKO1, TK9, YPPA1, UG1A, BLPPN8.2, YWKA1, BAU3.2, LPST1) and 2 reference strains (B. thuringiensis serovar kurstaki HD1 & B. thuringiensis serovar israelensis H14) were isolated and purified by standard method. 16S rRNA genes were amplified by PCR method using universal primers of 27f and 1529r. PCR products were digested by 4 restriction endonucleases (EcoR1, HindIII, Pst1 dan HaeIII), and separated by agarose electrophoresis method to generate ARDRA profiles. Results of study showed that only ARDRA profiles generated by Hae III digestion were found to be meaningful and therefore used to identify the isolates. The ARDRA profile analysis indicated that the reference strain of B. thuringiensis serovar kurstaki HD1 could be clearly separated with B. thuringiensis serovar israelensis H14. In fact, those two strains have been widely recognized to be different in terms of their pathogenic specifity against insects. B. thuringiensis serovar kurstaki HD1 has been known to be specifically pathogenic to Lepidopteran whereas B. thuringiensis serovar israelensis H14 has been known to be specifically pathogenic to Dipteran. Key words : application, ARDRA, indigenous, B. thuringiensis, C. binotalis  
Kemampuan Kitinase Streptomyces RKt5 sebagai Antijamur terhadap Patogen Fusarium oxysporum Yurnaliza, Yurnaliza; Margino, Sebastian; Sembiring, Langkah
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.537 KB) | DOI: 10.31258/jnat.14.1.42-46

Abstract

The purpose of the reasearch is to determine of antifungal activity from chitinase from Streptomyces RKt5 to inhibite growth of Fusariumoxysporum. The chitinase of Streptomyces RKt5 produced in liquid chitin medium with optimum conditions (inoculum concentration, pHand incubation time) and then partially purified with ammonium sulphate. The enzyme products were tested the antifungal activity againstF.oxysporum. The results showed that mycelial growth of F.oxysporum can be inhibited by Streptomyces RKt 5 in dual culture test. Thepartial purified chitinase enzyme couldn’t inhibit the fungal growth. But if the mycellium fragmented, the enzyme could degrade the fungalcell wall in incubation time. The frequency of fungal cell wall lysis and levels of N-acetylglucosamine released that have been increasingalong with the length of incubation time.
OPTIMASI KONDISI FERMENTASI UNTUK PRODUKSI SELULOSA BAKTERI OLEH STRAIN SLK-1 DALAM MEDIA DASAR AIR KELAPA (OPTIMIZATION OF FERMENTATION CONDITIONS FOR THE PRODUCTION OF BACTERIAL CELLULOSE BY SLK-1 STRAIN IN COCONUT WATER BASED MEDIUM) Sarkono, Sarkono; Moeljopawiro, Sukarti; Setiaji, Bambang; Sembiring, Langkah
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRAK   Tujuan penelitian ini adalah mengoptimasi kondisi fermentasi terbaik strain Bakteri Asam Asetat penghasil selulosa yaitu isolat SLK-1.  Strain ini diisolasi dari buah salak pada penelitian sebelumnya.  Hasil optimasi menunjukkan bahwa kondisi fermentasi optimum untuk pertumbuhan dan produksi selulosa pada isolat SLK-1  dicapai dengan sumber karbon gula pasir, sumber nitrogen ammonium sulfat, pH 7, suhu inkubasi 25°C dan metode fermentasi statis. Karakter struktur permukaan selulosa hasil fermentasi isolat SLK-1 dipengaruhi oleh metode fermentasi yang digunakan.  Metode fermentasi goyangan berpengaruh menurunkan produksi selulosa pada  isolat SLK-1 dan merubah struktur permukaan yaitu susunan mikrofibril lebih renggang dan membentuk gelembung.   Kata Kunci: bakteri asam asetat, optimasi, fermentasi, selulosa bakteri, penggoyangan
KEANEKARAGAMAN SPESIES BAKTERI PADA KULTUR DARAH WIDAL POSITIF ASAL KOTA SEMARANG BERDASARKAN KARAKTER FENOTIPIK Darmawati, Sri; Sembiring, Langkah; Asmara, Widya; T. Artama, Wayan
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRAK   Tujuan penelitian ini untuk menentukan keanekaragaman spesies bakteri pada kultur darah Widal positif  Asal kota Semarang berdasrkan karakter fenotipik. Sampel darah yang dikultur sebanyak 136 sampel berasal dari pasien rawat inap dan rawat jalan di 4 rumah sakit serta 2 puskesmas di kota Semarang (RSUD Kota Semarang, RSUD Tugurejo, RS. Islam Sultan Agung,  dan 2 Puskesmas yaitu Kedungmundu dan Bangetayu.  Kultur darah digunakan medium BacT/Alert FAN blood culture bottles (Biomerieux), subkultur digunakan medium Blood Agar Plate (BAP, OXOID) dan Mac Conkey (MC, OXOID), dilanjutkan  uji biokimia digunakan medium API 20E dan API 50CHB/E untuk identifikasi strain anggota familia Enterobacteriaceae serta APIStap (Biomerieux) untuk identifikasi spesies anggota Staphylococcus. Kultur darah positif sebanyak 59 sampel (43.4%) terdiri dari 44 sampel (32,4%) positif Staphylococcus sp. (S. aureus, S. saprophyticus, S. xylosus, S. warnei, S. hominis, S. cohnii) dan 15 sampel (11%) positif bakteri batang gram negatif anggota familia Enterobacteriaceae yaitu Enterobacter cloacae, S. typhi, Serratia marcescens, Escherichia coli, Salmonella ssp., Klebsiella pneumoniae ssp. Ozanae. Berdasarkan karakter fenotipik  bakteri batang gram negatif dapat dikelompokkan menjadi 4 kluster, kluster pertama beranggotakan S. typhi , kluster kedua beranggotakan E. coli dan Salmonella ssp., kluster ketiga beranggotakan Ser. Marcescens dan kluster keempat beranggotakan Enterobacter cloacae dan Kleb. pneumoniae ssp. Ozaenae. Bakteri kokus gram positif berdasarkan karakter fenotipiknya dapat dikelompokkan menjadi 6 kluster  yang tampak sangat bervariasi   Kata kunci:  Widal, Kultur darah, BacT/Alert FAN, API 20E, API 50 CHB/E, API Stap
UJI AKTIVITAS ANTIBAKTERI ISOLAT ACTINOMYCETES DARI RIZOSFER PADI (ORYZA SATIVA) TERHADAP SALMONELLA TYPHOSA DAN STAPHYLOCOCCUS AUREUS Ambarwati, Ambarwati; S, Tanti Azizah; Sembiring, Langkah; Wahyuono, Subagus
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

Actinomycetes merupakan anggota bakteri yang dipromosikan sebagai penghasil zat  antimikrobial  terbesar.  Tujuan penelitian ini adalah untuk mengisolasi dan mengidentifikasi Actinomycetes dari rizosfer padi (Oryza sativa) yang berpotensi sebagai penghasil antibakteri dan dapat menghambat pertumbuhan Salmonella typhosa dan Staphylococcus aureus. Penelitian ini menghasilkan 17 isolat Actinomycetes, 8 isolat diantaranya mampu menghasilkan zat antibakteri. Diantara 8 isolat tersebut, satu isolat mampu menghambat pertumbuhan kedua bakteri uji, yaitu NRPR 13 yang menghambat Salmonella typhosa (dengan diameter daerah hambatan 14 mm) dan Staphylococcus aureus (12 mm). Tiga isolat mampu menghambat pertumbuhan bakteri Salmonella  typhosa, yaitu NRPR  14  (11 mm), NRPR  62 dan NRPR 67 (masing-masing 10 mm). Dan empat isolat mampu menghambat pertumbuhan Staphylococcus aureus, yaitu RPR 15 (14 mm),  NRPR 11 (17 mm), NRPR 33 (11 mm) dan NRPR 46 (16 mm).  Berdasarkan hasil penelitian ini dapat disimpulkan bahwa Actinomycetes yang diisolasi dari rizosfer padi berpotensi menghasilkan zat antibakteri. Kata kunci : Actinomycetes,  Zat Antibakteri, S. typhosa,  S. aureus.
Optimasi Produksi Poli-β-Hidroksibutirat (PHB) oleh Bacillus sp. PSA10 Yanti, Nur Arfa; Margino, Sebastian; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.574 KB) | DOI: 10.24002/biota.v15i3.2587

Abstract

A new strain characterized as Bacillus sp. PSA10 was found to produce poly-β-hydroxybutyrate (PHB) at concentration of 52.28% (g PHB/g dry cell weight) in shaken flask culture, using sago starch as a carbon source. This research is aimed to determine the optimum culture condition of PHB production Bacillus sp. PSA10 at laboratory scale. Optimization of PHB production was conducted in this research, in terms of inoculum concentration, concentration of the major components in minimal medium, environmental condition and incubation time. The result showed that optimum conditions for the production of PHB by Bacillus sp. PSA10 were achieved at minimal medium (Ramsay medium) with 5% (v/v) inoculum concentration, 2% (w/v) sago starch, 1.0 g/l (NH4)2SO4, 6.7 g/l Na2HPO4.7H2O, and 0 g/l KCl. The optimum environmental conditions were achieved with initial pH 7, temperature 37oC, agitation speed at 150 rotary per minute (rpm) and the best of incubation time was 48 hour. Under this optimum condition, the maximum PHB production by Bacillus sp. PSA10 increased from 52.28% to 71.35% (g PHB/g dry cell weight) at 48 hour cultivation. Therefore, Bacillus sp. PSA10 is potential to apply for PHB production from sago starch at industrial scale.
Uji Patogenisitas Isolat Bakteri Indigenous (Bacillus thuringiensis) terhadap Serangga Hama Kubis (Crocidolomia binotalis Zell) Salaki, Christina L.; Situmorang, Jesmandt; Sembiring, Langkah; Handayani, Niken
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.033 KB) | DOI: 10.24002/biota.v14i3.2582

Abstract

Pathogenicity of 34 indigenous B. thuringiensis isolates against C. binotalis were determined. The pathogenicity test was conducted by using leaf dipped method with various spore concentrations. Third instar larvae of C. binotalis were used as insect test. Mortality data of test larvae were used to determine the pathogenicity of the isolates in terms of 72 hours LC50 by using probit analysis. The results of experiments showed YPPA 1. was the most pathogenic isolate, producing 72 hours LC50 = 9.5 x 103 spore.ml-1 with LT50 (1.5 x 107 spore.ml-1) of 24.6 hours while the ACH 2.3 was found to be the least pathogenic isolate with 72 hours LC50 = 2.3 x 106 spore.ml-1 and LT50 (1.5 x 107 spoore.ml-1) of 40.7 hours. The shortest LT50 (1.5 x 107 spore.ml-1 was found to be 18.2 hours produced by TUS.1 with 72 hours LC50 = 3.9 x 105 spore.ml-1 whereas the longest LT50 (1.5 x 107 spore.ml-1) was found tobe 83.2 hours produced by the SLK 4.1 with 72 hours LC50 = 3.1 x 104 spore.ml-1. Therefore, it can be concluded that both YPPA.1 and TUS.1 isolates are potential candidate to be developed for biological control agent.
Seleksi, Karakterisasi dan Identifikasi Bakteri Pendegradasi 2-(thiocyanomethylthio) benzothiazole (TCMTB) Sembiring, Langkah; Susilawati, Lela; Suhartanti, Dwi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.973 KB) | DOI: 10.24002/biota.v13i3.2565

Abstract

The objective of this research was to investigate the capabilities of bacteria isolated from industrial tanning waste to degrade TCMTB. The bacteria was initialy screened, based on their tolerance to various concentration of TCMTB using paper disk method. Then, those strains were further analyzed in terms of their ability to produce ammonia (NH4+) and sulphate (SO42-). Degradation activity was measured based on remaining residue of TCMTB analyzed using HPLC. The superior strain that showed the highest activity in degradation of TCMTB then were characterized and identified based on phenotypic and 16S rDNA sequence analysis. The result of the experiments showed that four selected strains among seven were choosen based on their high tolerance to various concentration of TCMTB, namely PK1, PK2, PK4 and PK6. All four strains showed the ability to produce ammonia and sulphate but three of which, namely PK2, PK4 and PK6 showed the high capability to degrade TCMTB. One particular strain (PK2) was observed to degrade TCMTB 40.8% within 7 days, but the others were less than 30%. Based on the phenotypic characteristics and 16S rDNA sequence analysis, the best strains (PK2) was identified to be member of genus Pseudomonas.
Co-Authors A.A. Ketut Agung Cahyawan W Ambarwati Artama, I Wayan Tunas Bambang Setiaji C. J. Soegihardjo Charlie Ester de Fretes, Charlie Ester Christina L. Salaki Dwi Suhartanti Elisa Nurnawati Endang Sutriswati Rahayu Helen Joan Lawalata Jamhari Jamhari Jesmandt Situmorang Jusup Subagja, Jusup Laksono Trisnantoro Lela Susilawati Lisa Lisdiana Masashi Kawaichi, Masashi Niken Handayani Nur Arfa Yanti, Nur Arfa Nurhayani H. Muhiddin, Nurhayani H. Orryani Lambui S. Rahayu, S. Sarkono Sarkono Sebastian Margino Sri Darmawati Subagja, Yusup Subagus Wahyuono Sukarti Moeljopawiro Tanti Azizah S Tri Ardyati Wayan T. Artama Wayan T. Artama Widayati, Wiwik E. Widya Asmara Yekti Asih Purwestri Yurnaliza, Yurnaliza