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All Journal HAYATI Journal of Biosciences Jurnal Hortikultura Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bumi Lestari Zuriat Jurnal Ilmiah Aplikasi Isotop Radiasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pelita Perkebunan (Coffee and Cocoa Research Journal) AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Indonesian Journal of Biotechnology Biology, Medicine, & Natural Product Chemistry Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology ANNALES BOGORIENSES Jurnal Pertanian Agros Biosaintifika: Journal of Biology & Biology Education Scripta Biologica JURNAL ILMIAH AGRINECA Agrosaintifika: Jurnal Ilmu-Ilmu Pertanian
Endang Semiarti
Fakultas Biologi, Universitas Gadjah Mada, Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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PENGARUH CAHAYA DAN TEMPERATUR TERHADAP PERTUMBUHAN TUNAS DAN PROFIL PROTEIN TANAMAN ANGGREK Phalaenopsis amabilis TRANSGENIK PEMBAWA GEN Ubipro::PaFT Putra, Rinaldi Rizal; Mercuriani, Ixora Sartika; Semiarti, Endang
Bioeksperimen: Jurnal Penelitian Biologi Vol 2, No 2: September 2016
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v2i2.2483

Abstract

Penelitian ini bertujuan untuk mencari kondisi yang tepat dalam percepatan pembungaan tanaman Phalaenopsis amabilis transgenik yang telah disisipi gen penentu waktu pembungaan Ubipro::PaFT. Metode penelitian ini menggunakan tanaman transgenik pembawa gen Ubipro::PaFT umur 18 bulan setelah penanaman. Tanaman ditumbuhkan pada inkubator dengan pencahayaan menggunakan lampu LED putih dan kombinasi LED putih biru, dengan fotoperiodisitas 8 jam terang 16 jam gelap, suhu 25ºC pada fase terang dan 20ºC pada fase gelap selama 20 minggu. Setelah 20 minggu pertumbuhan tanaman, dilakukan analisis profil protein dengan metode SDS-PAGE untuk mengetahui protein yang diproduksi pada setiap fase pertumbuhan yang diamati.Hasil penelitian menunjukkan kombinasi cahaya LED putih dan biru meningkatkan pembentukan daun sebesar 60%, panjang daun 70,58%, tetapi belum diperoleh kemunculan infloresen. Analisis profil protein menunjukkan terbentuknya protein dengan ukuran 108,57; 71,30; 56,16; 40,85; 26,79; 13,27; dan 13,12 kilodalton pada tanaman transgenik, tetapi tidak terdeteksi protein dengan ukuran 19,65 kDa yang sesuai dengan berat molekul protein PaFT, sementara protein dengan ukuran sekitar 56,16 kDa sesuai dengan berat molekul protein POH1(Phalaenopsis Orchid Homeobox1). Hal ini menunjukkan bahwa gen vegetatif POH1 mampu menghambat aktivasi gen PaFT pada tanaman P. amabilis transgenik umur 20 minggu, sehingga tanaman masih dalam fase juvenil dan belum mampu diinduksi untuk berbunga.
Improvement of Genetic Transformation Efficiency in Vanda tricolor Orchid Using Acetosyringone Dwiyani, Rindang; Purwantoro, Azis; Indrianto, Ari; Semiarti, Endang
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/53

Abstract

Vanda tricolor  Lindl. var. suavis  is an Indonesian wild orchid which is now extremely rare in nature due to its habitat  destruction.  Development  of  an  appropriate  method  for  improving   Vanda  orchid  through  genetic modification could be valuable for horticulture and, indirectly, also for conservation. In this research, a method of  Agrobacterium-mediated transformation of two  Vanda  tricolor  obtained  from  Salak Mount, West Java and merapi Mount, Yogyakarta in Indonesia protocorms was improved using acetosyringone (AS). Concentrations of 0 and 25 ppm AS were used in transformation of pG35S binary vector containing kanamycin resistance gene into V.  tricolor  protocorms.  The  result  showed  that  25  ppm  AS  was  required  on  innoculation  with Agrobacterium  solution, without AS on cocultivation. Five week s after  treatment  on the 300  ppm kanamicyncontaining medium, green protocorms were obtained, that  was  11.01% for  V.  tricolor  from Salak Mount with pre-culture treatment prior to innoculation, 9.39% for  V.  tricolor  from Merapi  Mount with pre-culture treatment prior to  innoculation,  and  1.37%  for  V.  tricolor  from  Merapi  Mount  without  pre-culture  treatment  prior  to innoculation. The  best  condition  to  set  high  efficiency  of  transformation  is  pre -culture  protocorms  prior inoculation, soaking protocorm on 25  ppm  AS for 30 minutes, then cocultivate its on AS-free  callus induction medium Key words: Vanda tricolor, Agrobacterium, orchid protocorms, acetosyringone
Karakterisasi Isolat Rhizoctonia sp. Patogenik dan Rhizoctonia Mikoriza Pada Tanaman Anggrek Tanah Spathoglottis plicata Soelistijono, Soelistijono; Priyatmojo, Achmadi; Semiarti, Endang; Sumardiyono, Christanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.536 KB) | DOI: 10.24002/biota.v16i2.121

Abstract

Rhizoctonia mikoriza merupakan jamur yang mampu berasosiasi dengan anggrek tanah. Selain sebagai mikoriza, terdapat isolat Rhizoctonia sp. patogen dan penyebab penyakit busuk akar pada Spathoglottis plicata. Penelitian ini bertujuan mengetahui perbedaan antara Rhizoctonia sp. patogen dan Rhizoctonia mikoriza secara morfologi dan molekular menggunakan teknik RAPD. Hasil penelitian secara morfologi menunjukkan bahwa warna koloni, panjang dan jumlah inti sel isolat Rhizoctonia sp. patogen dan Rhizoctonia mikoriza pada S. plicata tidak berbeda, tetapi berbeda pada ketebalan sel dan pengelompokan isolat berdasarkan uji anastomosis hifa. Teknik molekuler RAPD menunjukkan bahwa setiap isolat Rhizoctonia sp. patogen dan Rhizoctonia mikoriza memiliki perbedaan pada struktur DNA.
Micropropagation of Dendrobium phalaenopsis Orchid Through Overexpression of Embryo Gene AtRKD4 Setiari, Nintya; Purwantoro, Aziz; Moeljopawiro, Sukarti; Semiarti, Endang
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.1690

Abstract

To increase the efficiency of crop production from Dendrobium phalaenopsis orchids, mass propagation has been performed by inducing somatic embryogenesis through Agrobacterium-mediated transformation of the Arabidopsis embryo gene AtRKD4 into orchid protocorm (developing orchid embryo). The three-week-old protocorms of D. phalaenopsis were genetically transformed with T-DNA carrying 35S :: GAL4 :: AtRKD4 :: GR through A. tumefaciens strain EHA 105. The cultures were maintained in VW medium with 10 mg L-1 Hygromycin. Due to the existence of glucocorticoid response element (GR) in the T-DNA construct, the transformed protocorms were transferred into VW medium with the addition of 15 μM Dexamethasone in 6 weeks after transformation to activate the transgene. A total of 12% protocorms has been confirmed for Hyg + by using PCR. The expression of embryo gene AtRKD4 was confirmed by cDNA analysis using AtRKD4 specific primers and Actin primers as a positive control experiment. The expression level of AtRKD4 in 2.5-month-old D. phalaenopsis transformant shoots was 7 times higher than non-transformant plants, and increased to 86 times higher in 8-months, that much higher than that of non-transformant. These results provide an improved method for genetic transformation of D. Phalaenopsis and will (eventually) increase production efficiency in the future.
INDUCTION AND GROWTH KINETICS CALLUS OF TOMATO (SOLANUM LYCOPERSICUM) Setiaji, Arkan; Annisa, RR Rifka; Rumiyati, Rumiyati; Semiarti, Endang
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.21704

Abstract

Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ?Permata?) for optimum callus induction. Seeds were grown on ¼ MS medium for 10-15 days. Hypocotyl was excised and cultured on MS medium + 2 mg/l 2,4-D for 15 days as the explants for callus induction. Callus was transferred to MS medium with 8 variations of PGRs including the combination of BAP + NAA, and 2,4-D. Both fresh and dry weight was measured every 5 days over 60 days to establish the growth kinetics and growth efficiency of callus. Anatomic characters of calli were examined through paraffin-embedded method. The result showed of MS medium supplemented with 2.0 mg/l NAA and 0.2 mg/l BAP is optimum for tomato callus induction, based on highest number of the absolute growth rate on fresh weight (73.77% per day), dry weight (3.84% per day), and callus initiation time (5.56 days) achieved by the medium. Cells in the ground tissue of tomato hypocotyl are competent to be dedifferentiated into a callus. This research results were expected to find out suitable methods for tomato callus production in preparation for skincare uses.
Morphological And Biochemical Responses Of Saccharum Spontaneum L. Accessions To Drought Stress Munawarti, Aminatun; Taryono, Taryono; Semiarti, Endang; Sismindari, Sismindari
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germplasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objectives of this experiment were to evaluate the effect of drought stress initiated at vegetative growth stage on growth, leaf proline content and protein pattern of seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, and BOT-88). The plants were propagated from single node stalk segments in polybag in the field under non-stress condition for two months. The two month-old plants were then subjected to drought stress by withholding watering for eight weeks. Untreated control plants were watered every two days. Results indicated that drought stress reduced plant height, stalk diameter and green leaf number. On the other hand, there was a little difference between drought-stressed and control plants in terms of proline content. The protein pattern showed that drought stress caused a change in gene expression in the form of induction or repression of protein expression. A specific protein with a low range of molecular weight (Rf value about 0.647) showed constitutively expressed in accession BOT-53 but drought-inducible expressed in BOT-54. Keywords: Drought stress, glagah, proline, protein pattern, Saccharum spontaneum
The Influence of Thidiazuron on Direct Somatic Embryo Formation from Various Types of Explant in Phalaenopsis amabilis (L.) Blume Orchid Windi Mose; Ari Indrianto; Aziz Purwantoro; Endang Semiarti
HAYATI Journal of Biosciences Vol. 24 No. 4 (2017): October 2017
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1348.365 KB) | DOI: 10.4308/hjb.24.4.201

Abstract

Phalaenopsis amabilis is an important national flower of Indonesia as a parent for orchidbreeding, so that needs a good strategy to produce high number of plants. The objective of this research is to analyze the use of thidiazuron (TDZ) for producing high number of plantlets, through directly induction of somatic embryos (SEs) from various explants. The method was used 20 each of protocorms, leaves, stems and roots as explants. The explants were dissected transversely, then put on various culture media: New Phalaenopsis (NP) and NP + (1, 2, 3) mgL−1 TDZ. Cultures were maintained at 25°C with continous white light. The formation of SEs was observed every week for 8 weeks. The results showed that SEs formation increased inline with the addition of TDZ concentration to the NP medium, for both velocity and amount of SEs formation. In NP0, SEs were formed at (26.07 ± 0.73) days after inoculation of protocorm, whereas on NP + (1, 2, and 3 mgL−1) TDZ, SEs were formed at (17.85 ± 0.67) days, (15 ± 0.64) days, and (11 ± 0.64) days, respectively. All types of explants formed SEs on NP + TDZ (1–3 mgL−1), whereas only 14 of 20 protocorms produced SEs (70%), and 8 of 20 stems formed SEs (40%) in NP0. In roots, SEs was formed on NP + 2 mgL−1 TDZ and NP + 3 mgL−1 TDZ. For stems, the highest amount of SEs (28.25 ± 1.07) was reached on NP + 3 mgL−1 TDZ, followed by protocorm (23.30 ± 1.13) SEs and roots (8.25 ± 0.68) SEs. In contrast, in NP0, the amount of SEs was very low (1.25 ± 0.46) from stem and (1.50 ± 0.65) from protocorms, there was no evidence of SEs formation in the leaves and roots.
In Vitro Germination and Flowering of Dendrobium capra J.J. Smith, An Endemic Orchid of Java Muhammad Dylan Lawrie; Zulfa Layina; Della Rosiana Ningtias; Falah Nur Alifianto; Ari Indrianto; Aziz Purwantoro; Endang Semiarti
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.172

Abstract

Dendrobium capra is an Indonesian endemic orchid species that live in Java. It grows on low altitude with warm climate. D. capra has beautiful small yellow greenish flower that grow in raceme inflorescence. This orchid faces a threat in its natural habitat due to having a long life cycle and a forestry main commodity as a main host thus categorized as Appendix II on CITES list. To address that problem, ex situ conservation approach using in vitro culture method is necessary. Germination enhancement effort using complex organic substances found that 200 ml/l tomato extract gave best germination result. Analysis on D. capra plantlet growth also showed that MS medium produced better plantlet size than NP, VW and KC medium. Supplementing medium with a combination of NAA and TDZ has also successfully induced early flowering within 11 month of culture period. This information is important to achieve successful in vitro culture of D. capra for various purposes.
Phenotype and genotype characterization of Phalaenopsis amabilis (L.) Blume Orchid Transformant Harboring Construct UBI::Cas9::U3::PDS3 Amarilis Andani Kesuma; Sri Nopitasari; Yasushi Yoshioka; Shogo Matsumoto; Endang Semiarti
Jurnal Hortikultura Indonesia Vol. 11 No. 3 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.3.212-220

Abstract

Phalaenopsis amabilis (L.) Blume adalah tanaman hias “Puspa Pesona Indonesia” yang dapat ditingkatkan kualitasnya dengan teknik rekayasa genetika. Transformasi genetik dengan perantara Agrobacterium tumefaciens dan CRISPR/Cas9 digunakan dalam penelitian ini untuk pengeditan genom secara lebih spesifik dan presisi pada target sekuen gen PHYTOENE DESATURASE3 (PDS3) yaitu gen yang berperan penting pada biosintesis kloroplas. Dalam penelitian ini digunakan tanaman transforman umur 12 bulan yang ditumbuhkan dari protokorm yang telah diintegrasi dengan T-DNA pembawa konstruksi UBI::Cas9::U3::PDS3/plasmid pRGEB32. Pembuktian tanaman transforman tersebut masih mengandung konstruksi T-DNA tersebut perlu dilakukan, yaitu dengan karakterisasi secara genotipe dan fenotipe. Tujuan penelitian ini adalah untuk mengkarakterisasi P. amabilis transforman pembawa T-DNA dengan konstruksi UBI::Cas9::U3::PDS3 secara genotip dan fenotip dibandingkan dengan P. amabilis non-transforman. Karakterisasi genotipe dilakukan dengan mendeteksi integrasi T-DNA pembawa konstruksi UBI::Cas9::U3::PDS3 pada genom anggrek P. amabilis menggunakan beberapa primer yaitu HPT, Cas9, PDS3 dan trnL-F (primer kontrol internal). Analisis karakter fenotipe dilakukan dengan pengamatan morfologi dan analisis kadar klorofil menggunakan metode spektrofotometri. Hasil penelitian menunjukkan bahwa genom anggrek P. amabilis transforman pembawa konstruksi UBI::Cas9::U3::PDS3 umur 12 bulan dapat teramplifikasi oleh semua primer. Analisis fenotipe P. amabilis transforman menunjukkan adanya perubahan warna tanaman dari hijau menjadi albino dengan kadar klorofil lebih rendah jika dibandingkan dengan P. amabilis non-transforman. Hal ini menunjukkan bahwa teknologi CRISPR/Cas9 dapat digunakan untuk mengedit genom tanaman anggrek. Kata kunci: Anggrek, CRISPR/Cas9, klorofil, Phalaenopsis amabilis (L.) Blume, PHYTOENE DESATURASE 3 (PDS3), Transforman
PRODUKSI GALUR MURNI MELALUI INDUKSI EMBRIOGENIK MIKROSPORA CABAI MERAH BESAR DENGAN STRES Ari Indrianto; Endang Semiarti; , Surifah
Zuriat Vol 15, No 2 (2004)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v15i2.6801

Abstract

Induksi embriogenik mikrospora cabai merah besar dapat dilakukan dengan stres panas dan pelaparan (starvasi karbohidrat). Stres diperlukan untuk mengubah perkembangan gametofitik (mikrospora membelah asimetrik) kearah sporofitik (mikrospora membelah simetrik) untuk membentuk embrio. Mikrospora pada stadium uninukleat akhir dikulturkan secara aseptik didalam medium starvasi karbohidrat (medium B) masing-masing pada 25oC; 33oC dan 35oC selama 2; 4 dan 6 hari. Setelah praperlakuan stres, mikrospora disubkultur kedalam medium embriogenesis (medium A2) tanpa zat pengatur tumbuh dan diinkubasi pada suhu 25oC. Pengamatan sitologis terhadap tipe pembelahan inti dan mikrospora multiseluler (proembrio) dilakukan dengan pengecatan DAPI. Hasil penelitian menunjukkan, praperlakuan starvasi selama 4 hari dapat menginduksi 3.4% dari populasi mikrospora menjadi embriogenik, dimana 4% diantaranya tumbuh menjadi proembrio. Tambahan stres panas 33oC selama 4 hari meningkatkan persentase induksi embriogenik mikrospora menjadi 29%, dimana 30% diantaranya berkembang menjadi proembrio. Induksi embriogenik mikrospora cabai merah besar merupakan langkah awal untuk memproduksi tanaman haploid, penggandaan kromosom selanjutnya akan menghasilkan tanaman dobel haploid yang merupakan galur murni.
Co-Authors , Surifah A.A. Ketut Agung Cahyawan W Achmadi Priyatmojo Aditya Nur Subchan Agus Slamet Ahmad Suyoko Alisa Julia Nurulita Amarilis Andani Kesuma Aminatun Munawarti Aminatun Munawarti, Aminatun Amru Rizal Basri Anami Riastri Annisa, RR Rifka Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Aries Bagus Sasongko Asri Fajar Milasari Azis Purwantoro Azis Purwantoro Aziz Purwantoro Bekti Sulistya Utami Binti Tsulsiyah Brilliant Kharisma Apritadila Budi Setiadi Daryono BUDI SETIADI DARYONO Cahya Lembayung Sutra Catharina Tri Widyastuti Christanti Sumardiyono Christanti Sumardiyono Della Rosiana Ningtias Devi Bunga Pagalla Dewi Tika Sari Dewi Yuliana Rizqi Dika Sundari Eka Fitriana Candra Ningrum Evilili Usmanti Exsyupransia Mursyanti Exsyupransia Mursyanti Exsyupransia Mursyanti Faiza Senja Widya Perdana Falah Nur Alifianto Febri Yuda Kurniawan Fhea Putri Cristy Fitriana Puspitasari Frisca Damayanti Gde Cahyadi Wirajagat Ikhsanudin Nur Rosyidi Ixora Sartika Mercuriani JAKA WIDADA Jose Gutierrez-Marcos Jose Gutierrez-Marcos Kana Ninomiya Maryani Maryani Maura Indria Meidianing Muhammad Dylan Lawrie Muhammad Dylan Lawrie Muhammad Dylan Lawrie Muhammad Dylan Lawrie Naufal Ghozi Aditya Perdana Ni Luh Putu Kayika Febryanti Nintya Setiari Nintya Setiari Oedjijono Oedjijono Oktaviana Herawati Putra, Rinaldi Rizal Rahayu Sulistianingsih Rina Arimarsetiowati Rinaldi Rizal Putra, Rinaldi Rizal Rindang Dwiyani Rizka Riliant Puspasari Rumiyati Rumiyati Rumiyati Rumiyati S. Soelistijono Seonghoe Jang Setiaji, Arkan Shogo Matsumoto Shogo Matsumoto Sismindari . Sismindari Sismindari Sismindari Sismindari Sitarina Widyarani Soelistijono Soelistijono Soenghoe Jang Sri Nopitasari Sri Nopitasari Sri Wahyuningsih Stalis Norma Ethica Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sulastri Isminingsih Taryono, Taryono Thoyibatul Farida Tri Joko Raharjo Triono Bagus Saputro, Triono Bagus Umi Kulsum Nur Qomariah Windi Mose Windi Mose Woerjono Mangoendidjojo Woro Anindito Sri Tunjung Yasushi Yoshioka Yasushi Yoshioka Yohana Theresia Maria Astuti Yuli Setiawati Yuuki Asano Zulfa Layina