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Profile of Interleukin-4 and Interferon-γ of Balb/c Mice after Salivary Gland Extract of Anopheles sundaicus s.l. Vaccination and Infected by Plasmodium berghei-ANKA Machrus, Ali; Adrial, Adrial; Armiyanti, Yunita; Wiyono, Hidayat Teguh; Senjarini, Kartika
Jurnal ILMU DASAR Vol 15 No 2 (2014)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.451 KB) | DOI: 10.19184/jid.v15i2.532

Abstract

Malaria infection is initiated when sporozoites are inoculated into a vertebrate host via the salivary glands of an Anopheles mosquito. During Anopheles bite,  the salivary glands release components that include vasomodulator and immunomodulators. The salivary components of vectors have important role in transmission of pathogen. Therefore, if these components were injected repeatedly into a vertebrate host, it can stimulate host immune system and inhibit the transmission of the pathogen into the host. This could be observed the increasing level of  IFN-γ and decreasing level of IL-4 in mice model  of malaria after vaccination with  salivary gland ekstract (SGE) from An sundaicus s.l. It has also been proven that this  mechanisms was related with pathogen of malaria. This was supported by the reduction of parasitemie rate in those mice model after infection by P. Berghei.   Keywords: An sundaicus s.l., IFN-γ, IL-4,immunomodulators, salivary gland , TBV
Immunogenity of Protein Extract from Salivary Gland of Anopheles aconitus in Malaria Endemic Area Septiawan, Mahful; Budayatin, Budayatin; Wiyono, Hidayat Teguh; Senjarini, Kartika
Jurnal ILMU DASAR Vol 18 No 1 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.462 KB) | DOI: 10.19184/jid.v18i1.2372

Abstract

Although malaria had ever been virtually eradicated from Indonesia but currently malaria is recognized as a serious re-emerging threat to public health. This disease is caused by malaria parasite which is transmitted to human host by Anopheles mosquitoes as main vector. It has been widely observed that saliva of mosquito that transmits disease contains several factors that could enhance pathogen infection. Therefore, it should be possible to control pathogen transmission by vaccinating the host against the molecule(s) in saliva that potentiate the infection. However, immunogenic specific component in mosquitoes vectors of Malaria has not yet been identified so far. The objective of this study are to analyze protein profile of SDS-PAGE and to know the immunogity the protein extract of salivary gland from potential vector of Malaria i.e. An. aconitus We used immunogenic reaction between salivary gland extract of these vectors against pool of human sera which were collected from endemic area. The reaction conducted by the dot-blot analyze. SDS-PAGE studies showed 15 major polypeptide bands of 284, 100, 84, 75, 66, 57, 53, 48, 45, 38, 33, 29, 15, 14, and 11 kDa. The dot-blot studies showed that the protein extract of salivary gland from An. aconitus are immunogenic.
Morphological Description of Drosophila melanogaster Wild Type (Diptera:Drosophilidae), Sepia and Plum Strain Hotimah, Husnul; Purwatiningsih, Purwatiningsih; Senjarini, Kartika
Jurnal ILMU DASAR Vol 18 No 1 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.395 KB) | DOI: 10.19184/jid.v18i1.3113

Abstract

Drosophila melanogaster is one of the insects which have a very important role in the development of the genetic science. Drosophila melanogaster have many mutation, recently there are many mutant such as sepia and plum strain. Morgan et al have found 85 strain mutan of Drosophila melanogaster. The purpose of these research was to characterize of morphological from the head, thorax, and abdomen. The result show that the morphological of Drosophilla melanogaster wild type, sepia and plum mutant have many similarities. The difference of them are the eyes color, Drosophila melanogaster wild type has red eye, sepia strain has a dark brown eye and plum has a dark purple eye.
Characterization Physiology and Molecular Bacteria Symbiont- Entomopathogenic Nematodes based of Gene Sequences Encoding the 16S rRNA District of Bromo Probolinggo Setiawan, Bagus; Sulistyanto, Didik; Senjarini, Kartika
Jurnal ILMU DASAR Vol 18 No 1 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.218 KB) | DOI: 10.19184/jid.v18i1.2723

Abstract

This study aims to identify entomopathogenic nematodes symbiotic bacteria phenotypically and based on the gene encoding 16S rRNA sequences. Bacterial symbionts of entomopathogenic nematodes, obtained from isolates from the area Wonokerto (WN01) and isolates Sukapura (SP01), Bromo, Probolinggo, two symbiont bacteria was found in entomopathogenic nematodes Steinernema sp. The method used in this study are: the isolation of entomopathogenic nematodes Steinernema sp. and bacterial symbionts conventionally for the identification of phenotypically, after the characterization of bacterial isolates, the isolation of genomic DNA, 16S rRNA PCR, DNA purification and DNA sequence analysis. The results based on phenotypic characterization showed that isolates WN01 and SP01, yellowish white, gram positive, negative bioluminenscene, catalase positive, can not hydrolyze urea, and also can not produce H2S. The results of the gene encoding 16S rRNA sequence can be deduced WN01 isolates have in common with the bacteria Bacillus strain toyonensis BCT 7112, while the SP01 isolates have in common with the bacteria Bacillus strain cereus ATCC 14 579.
KARAKTERISASI PARSIAL FAKTOR IMUNOMODULATOR KELENJAR SALIVA Aedes aegypti (DIPTERA: CULICIDAE) SEBAGAI KANDIDAT Transmission Blocking Vaccine (TBV) DEMAM BERDARAH DENGUE Wathon, Syubbanul; Senjarini, Kartika; Widajati, Sri Mumpuni Wahyu; Oktarianti, Rike
BERKALA SAINSTEK Vol 2, No 1 (2014)
Publisher : My Home

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penyakit Demam Berdarah Dengue (DBD) disebabkan oleh infeksi virus dengue yang dibawa Aedes aegypti (Ae. aegypti) sebagai vektor primernya. Pengendalian vektor pada penyakit DBD masih belum maksimal. Selain itu vaksin yang belisensi untuk penyakit DBD masih belum dilaporkan. Melalui pengembangan TBV salah satunya dengan memanfaatkan komponen saliva vektor. aliva vektor memiliki potensi dalam meningkatkan transmisi patogen ke tubuh inang, maka perlu adanya karakterisasi molekul dalam saliva nyamuk termasuk faktor imunomodulator. Karakterisasi faktor imunomodulator saliva Ae. aegypti dilakukan melalui uji reaksi silang antara protein kelenjar saliva Ae. aegypti dengan beberapa plasma darah manusia. Hasil penelitian menunjukkan adanya protein spesifik yang dikenali antibodi dalam plasma darah orang endemik dengan berat molekul ~ 37 kDa. Hal ini megindikasikan bahwa di dalam tubuh penduduk endemik telah mengembangkan antibodi anti-saliva Ae. aegypti yang diduga berperan penting dalam resistensi terhadap infeksi virus dengue. Kata Kunci: Ae. aegypti, DBD, faktor imunomodulator, kelenjar saliva, TBV
KARAKTERISASI PARSIAL FAKTOR IMUNOMODULATOR KELENJAR SALIVA Aedes aegypti (DIPTERA: CULICIDAE) SEBAGAI KANDIDAT Transmission Blocking Vaccine (TBV) DEMAM BERDARAH DENGUE Wathon, Syubbanul; Senjarini, Kartika; Widajati, Sri Mumpuni Wahyu; Oktarianti, Rike
BERKALA SAINSTEK Vol 2 No 1 (2014)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penyakit Demam Berdarah Dengue (DBD) disebabkan oleh infeksi virus dengue yang dibawa Aedes aegypti (Ae. aegypti) sebagai vektor primernya. Pengendalian vektor pada penyakit DBD masih belum maksimal. Selain itu vaksin yang belisensi untuk penyakit DBD masih belum dilaporkan. Melalui pengembangan TBV salah satunya dengan memanfaatkan komponen saliva vektor. aliva vektor memiliki potensi dalam meningkatkan transmisi patogen ke tubuh inang, maka perlu adanya karakterisasi molekul dalam saliva nyamuk termasuk faktor imunomodulator. Karakterisasi faktor imunomodulator saliva Ae. aegypti dilakukan melalui uji reaksi silang antara protein kelenjar saliva Ae. aegypti dengan beberapa plasma darah manusia. Hasil penelitian menunjukkan adanya protein spesifik yang dikenali antibodi dalam plasma darah orang endemik dengan berat molekul ~ 37 kDa. Hal ini megindikasikan bahwa di dalam tubuh penduduk endemik telah mengembangkan antibodi anti-saliva Ae. aegypti yang diduga berperan penting dalam resistensi terhadap infeksi virus dengue. Kata Kunci: Ae. aegypti, DBD, faktor imunomodulator, kelenjar saliva, TBV
Imunogenic Protein of Salivary Gland from Anopheles sundaicus Armiyanti, Yunita; Nuryady, Moh Mirza; Utomo, Sugeng Setyo; Sardjono, Teguh Wahju; Fitri, Loeki Enggar; Senjarini, Kartika
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Malaria is still a major problem for developing countries, including Indonesia. One approach to overcome this disease is prevention by vaccination. However, there is still no effective malaria vaccine that is applicable. The ideal malaria vaccine is a combination vaccine that can prevent the pre-erythrocytic cycle, the erythrocytic cycle and transmission process. Salivary vector-based vaccine has the potential to be developed as a malaria vaccine because it can prevent transmission process and also decrease the morbidity of the disease. Saliva from Anopheles contains vasomodulator and immunomodulatory components, that are required in the blood feeding process, but in the same time it could enhance the transmission of the malaria parasite. If the component in the salivary vector can increase pathogen infection, then vaccinating the host with its anti-substances can control the transmission of pathogens (Transmision Blocking Vaccine). Anopheles sundaicus is an important vector of malaria in coastal areas of Java, Bali, Sumatra, Kalimantan and West Nusa Tenggara islands. Repeated exposures of Salivary Gland Extract (SGE) from this vector have been proven to be able to decrease parasitemic rates in mouse model for malaria in our study. The objective of this research is to determine and localize the immunogenic protein from SGE of An. sundaicus as the first step for the characterization of its immunomodulatory component. Mosquito salivary gland protein profile of An.sundaicus was determine by SDS-PAGE. Determination of salivary glands immunogenic proteins was conducted by Western Blotting with IgG from people living from endemic area as primary antibody. Out of 15 bands appeared in SDS PAGE ranging from 24 kD to 138 kD, only two protein bands with  molecular weights of 68 and 37 kDa were the most immunogenic. Those immunogenic proteins were consistent recognized by pooled serum of people as well as by individual response. Keywords: malaria, saliva, vector, immunogenic protein, vaccine
KARAKTERISASI ISOLAT BAKTERI FIBRINOLITIK WU 021055* ASAL PERAIRAN PANTAI PAPUMA, JEMBER Sri Pananjung, Ajeng Maharani; Ulfa, Evi Umayah; Senjarini, Kartika; Arimurti, Sattya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 2, No 1 (2015): June 2015
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (892.11 KB) | DOI: 10.29122/jbbi.v2i1.528

Abstract

A blood clot (thrombus) in a blood stream is formed due to a circulatory system imbalance in the hemostasis which results in plug of blood vessels. The suppliy of nutrients and oxygen to the tissues is inhibited (ischemia) by the accumulation of thrombus and embolus in the blood vessel. This prosses is the main cause for further atherotrombotic diseases such as myocardial infraction and cerebral infraction. This disease could be overcome by thrombolytic therapy by using fibrinolytic protease enzyme. Fibrinolytic activity of protease enzymes have been studied from various species of bacteria. Bacterial isolate of WU 021055* obtained from Papuma coastal waters has demonstrated fibrinolytic activity. This research was aimed to identify the bacterial isolate through morphological characterization (colony and cell morphology), physiological characterization (indole test, carbohydrates fermentation test (glucose, lactose, sucrose and fructose), catalase test, starch hydrolysis test, and the pH effect test), and molecular identification using 16S rRNA. Based on those characterizations, the bacterial isolate of WU 021055* shows a high similarity to Bacillus aerius.Keywords: Atherotrombosis, fibrinolytic, identification, characterization, bacteria ABSTRAKBekuan darah (trombus) dalam peredaran darah terbentuk akibat ketidakseimbangan sistem sirkulasi dalam hemostasis yang menyebabkan penyumbatan pembuluh darah. Akumulasi trombus dan embolus pada pembuluh darah mengakibatkan suplai nutrisi dan oksigen ke jaringan terhambat (iskemia) dan bahkan kematian jaringan (infark). Pembentukan ini merupakan etiologi dari penyakit aterotrombosis seperti infark miokard dan infark serebral. Penyakit akibat trombosis ini dapat diatasi dengan terapi trombolitik dengan enzim protease fibrinolitik. Aktivitas enzim protease fibrinolitik telah diteliti dari berbagai spesies bakteri. Isolat bakteri WU 021055* asal perairan pantai papuma tampak memiliki aktivitas fibrinolitik. Pada penelitian ini dilakukan identifikasi isolat bakteri melalui karakterisasi morfologi (morfologi koloni dan sel), karakterisasi fisiologis (uji indol, uji fermentasi karbohidrat (glukosa, laktosa, sukrosa dan fruktosa), uji katalase, uji hidrolisis pati, dan uji pengaruh pH), dan identifikasi secara molekuler menggunakan 16S rRNA. Berdasarkan karakterisasi morfologi, fisiologi, dan marker 16S rRNA, isolat bakteri WU 021055* menunjukkan kemiripan yang tinggi dengan Bacillus aerius.Kata kunci: Aterotrombosis, fibrinolitik, identifikasi, karakterisasi, bakteri
AKTIVITAS PROTEOLITIK DAN FIBRINOLITIK ISOLAT BAKTERI DARI PERAIRAN PANTAI PAPUMA KABUPATEN JEMBER Setiawan, Arif; Arimurti, Sattya; Senjarini, Kartika; Sutoyo, Sutoyo
BERKALA SAINSTEK Vol 4 No 1 (2016)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v4i1.4457

Abstract

Berbagai jenis bakteri dari perairan pantai dapat dieksplorasi sebagai sumber penghasil senyawa penting. Sejauh ini, bakteri dari Perairan Pantai Papuma Kabupaten Jember yang memiliki aktivitas proteolitik dan fibrinolitik belum dilaporkan. Tujuan penelitian ini adalah menguji aktivitas proteolitik dan fibrinolitik pada sejumlah isolat bakteri. Metode penelitian meliputi uji aktivitas proteolitik menggunakan Media Skim Milk Agar (SMA) terhadap 23 isolat bakteri dan uji aktivitas fibrinolitik menggunakan Media Fibrin pada isolat yang diketahui memiliki aktivitas proteolitik. Metode uji aktivitas dilakukan secara semikuantitatif. Sebanyak 11 isolat bakteri memiliki aktivitas proteolitik. Isolat bakteri WU 021012* memiliki indeks proteolitik tertinggi yaitu 4,3. Isolat bakteri yang fibrinolitik diperoleh sebanyak 3 isolat. Isolat bakteri WU 021055* memiliki indeks tertinggi sebesar 11.
Deteksi Aktivitas Fibrinolitik Isolat Bakteri WU 021055* Asal Perairan Pantai Papuma Jember Menggunakan Zimografi Ulfa, Evi Umayah; Utarti, Esti; Afkarina, Izzay; Arimurti, Sattya; Senjarini, Kartika
Global Medical & Health Communication (GMHC) Vol 5, No 2 (2017)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v5i2.1914

Abstract

Bakteri merupakan sumber penting berbagai enzim termasuk enzim fibrinolitik. Enzim ini diperlukan untuk mendegradasi bekuan darah pada orang yang mengalami penyakit trombosis. Isolat bakteri WU 021055* asal Pantai Papuma Jember terbukti menghasilkan enzim fibrinolitik ekstraseluler. Penelitian ini bertujuan mengetahui ukuran protein yang memiliki aktivitas fibrinolitik dan mengidentifikasi karakteristik morfologi isolat WU bakteri WU 021055*. Penelitian ini dilakukan di Laboratorium Mikrobiologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Jember pada April–Agustus 2014. Aktivitas fibrinolitik presipitat protein (PP) ditentukan menggunakan metode fibrin plate agar dan zimografi fibrin. Ekstrak protein kasar (EPK) dipanen pada jam ke-12 dan dipresipitasi menggunakan amonium sulfat 80%. Hasil uji aktivitas fibrinolitik menggunakan fibrin plate agar menunjukkan presipitat memiliki aktivitas fibrinolitik lebih besar dibanding dengan EPK. Dari hasil karakterisasi PP menggunakan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) diperoleh 11 pita protein dengan ukuran 12–41 kDa. Berdasar atas hasil zimografi fibrin, pita protein dengan berat molekul 24 kDa yang memberikan aktivitas fibrinolitik. Protein dengan ukuran 24 kDa ini mampu mendegradasi substrat fibrin. Simpulan, isolat bakteri WU 021055* mengandung berbagai protein ekstraseluler, memiliki bentuk koloni bulat berwarna putih dan termasuk bakteri gram prositif berbentuk batang.DETECTION OF FIBRINOLYTIC ACTIVITY OF WU 021055* BACTERIAL ISOLATE FROM PAPUMA BEACH COASTAL JEMBER USING ZYMOGRAPHYBacteria were important resources for various enzymes including fibrinolytic enzymes. This enzyme is  capable of degrading fibrin clot in patient with thrombotic diseases. Bacterial isolate of WU 021055* from Papuma Beach Coastal Jember could secrete extracellular fibrinolytic enzymes. The objective of this reasearch was to determine the molecular weight of protein responsible for fibrinolytic activity and to identify morphologycal characterization of bacterial isolate of WU 021055*. This study was conducted at Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Universitas Jember in April–August 2014. Fibrinolytic activity of precipitate protein (PP) was determined by using fibrin plate agar and fibrin zymography. Crude protein extract (CPE) was harvested at 12 hours and precipitated by 80% ammonium sulphates. The result of fibrinolityc activity determination showed that fibrinolytic activity of PP was higher than CPE. Protein characterization of PP by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) obtained 11 different protein bands corresponds to value 12–42 kDa. Based on fibrin zymography, the 24 kDa protein might contribute to fibrinolytic activity due to degraded fibrin substrates. In conclusion, bacterial isolate of WU 021055* contained extracellular fibrin protein was white colony and gram positives bacilli able to degraded.