Articles
<![CDATA[The Diversity of Potential Malaria and Dengue Mosquito Vector from Bangsring Village Wongsorejo District Banyuwangi East Java ]]>
<![CDATA[Siti Fat'hiyatul Azkiyah]]>;
<![CDATA[Kartika Senjarini]]>;
<![CDATA[Rike Oktarianti]]>;
<![CDATA[Hidayat Teguh Wiyono]]>;
<![CDATA[Syubanul Wathon]]>
<![CDATA[ Jurnal ILMU DASAR Vol 22 No 1 (2021) ]]>
Publisher : <![CDATA[Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember ]]>
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DOI: 10.19184/jid.v22i1.13601
<![CDATA[Bangsring village in Wongsorejo regency has been reported as malaria endemic area in Banyuwangi since 2011. Understanding the diversity and behavior of mosquito vector in this area will be very impotant in developing vector control program. The masquitoes were collected by landing collection outside and inside house by human bites, we also observed the area around cattle cage. During observation periods, a total 633 masquitos were collected consist of 44% malaria potential vector (Anopheles spp.) 19% Dengue fever (DF) potential vector (Aedes spp.) and 36% were not belong to both of them. Out of total collected Anopheles spp. mosquitoes about 65% were indentified as An. vagus, 25% were An. indevinitus, 8% were An. vagus (limosus), and only 2% as well as 0,04 % were An. supictus and An. kochi. Meanwhile we found 19% of Aedes sp. were Ae. aegypti and 81% were Ae. albopictus. This study showed that the dominan potential malaria’s vector is An. Vagus and the dominan DF vector is Ae. albopictus. Mostly colleted Anopheles sp. were exophagic and zoophilic with the highest activities between 06.00-08.00 pm. This was in contras to Aedes sp. where mosfly found endophilic and it has 2 hightime of activities Aedes sp. between 06.00- 07.00 AM and 05.00-06.00 PM. Keywords: Dengue, Malaria, diversity, Anopheles, Aedes.]]>
<![CDATA[Are Bacteria The Main Producers of Hydrolytic Enzymes in Aquatic Environment ]]>
<![CDATA[Kartika Senjarini]]>;
<![CDATA[Ulf Karsten]]>;
<![CDATA[Rhena Schumann]]>
<![CDATA[ Jurnal ILMU DASAR Vol 9 No 1 (2008) ]]>
Publisher : <![CDATA[Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember ]]>
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<![CDATA[Bacteria play an important role in the decomposition of organic matter, which is a key process in aquatic microbial food webs as well as its application in water bioremediation processes. DOM (Dissolved Organic Matter) is the major fraction of organic matter in most aquatic environments. Most of DOM is present as high molecular weight compounds that cannot be taken up by bacteria directly. Therefore, they must be hydrolysed enzymatically to be transported across microbial cell membranes. Molecular fluorosensors have been used as artificial substrates to study hydrolytic enzymes in situ. The present study wants to investigate the following hypothesis i.e. bacteria are the main producers of hydrolytic enzyme in aquatic systems. There were not any significant correlations between bacteria and the investigated hydrolases (esterase, peptidase, and β-glucosidase) in the meso- to eutrophic aquatic systems near Rostock city – North East Germany, although a wide range of bacterial abundances were covered. Enzyme saturations were not detected in all samples which may be explained by a high KM indicating a low affinity of enzymes (1) and or the contribution of many enzymes with different kinetics to the respective substrate degradation (2). There is also increasing evidence that bacteria may not be the sole or dominant source of esterases, peptidases and β-glucosidases in aquatic ecosystems. Invertebrate, fungi and other eukaryotes (diatoms, protozoa etc.) must be considered as possible and even important producers of hydrolytic enzymes. Several other factors may influence the correlations of bacteria to hydrolytic enzyme activities, i.e. variations in the species composition (1), a wide ranged variability of hydrolytic activities influenced directly by other substrates (low enzyme affinity), element availability (N and P), temperature and other abiotic factors (2), the existence and persistence of enzymes caused by other (passive) processes (e.g. cell lysis) (3).]]>
<![CDATA[Profile of Interleukin-4 and Interferon-γ of Balb/c Mice after Salivary Gland Extract of Anopheles sundaicus s.l. Vaccination and Infected by Plasmodium berghei-ANKA ]]>
<![CDATA[Ali Machrus]]>;
<![CDATA[Adrial Adrial]]>;
<![CDATA[Yunita Armiyanti]]>;
<![CDATA[Hidayat Teguh Wiyono]]>;
<![CDATA[Kartika Senjarini]]>
<![CDATA[ Jurnal ILMU DASAR Vol 15 No 2 (2014) ]]>
Publisher : <![CDATA[Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember ]]>
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DOI: 10.19184/jid.v15i2.532
<![CDATA[Malaria infection is initiated when sporozoites are inoculated into a vertebrate host via the salivary glands of an Anopheles mosquito. During Anopheles bite, the salivary glands release components that include vasomodulator and immunomodulators. The salivary components of vectors have important role in transmission of pathogen. Therefore, if these components were injected repeatedly into a vertebrate host, it can stimulate host immune system and inhibit the transmission of the pathogen into the host. This could be observed the increasing level of IFN-γ and decreasing level of IL-4 in mice model of malaria after vaccination with salivary gland ekstract (SGE) from An sundaicus s.l. It has also been proven that this mechanisms was related with pathogen of malaria. This was supported by the reduction of parasitemie rate in those mice model after infection by P. Berghei. Keywords: An sundaicus s.l., IFN-γ, IL-4,immunomodulators, salivary gland , TBV]]>
<![CDATA[Deteksi Aktivitas Fibrinolitik Isolat Bakteri WU 021055* Asal Perairan Pantai Papuma Jember Menggunakan Zimografi ]]>
<![CDATA[Evi Umayah Ulfa]]>;
<![CDATA[Esti Utarti]]>;
<![CDATA[Izzay Afkarina]]>;
<![CDATA[Sattya Arimurti]]>;
<![CDATA[Kartika Senjarini]]>
<![CDATA[ Global Medical & Health Communication (GMHC) Vol 5, No 2 (2017) ]]>
Publisher : <![CDATA[Universitas Islam Bandung ]]>
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DOI: 10.29313/gmhc.v5i2.1914
<![CDATA[Bakteri merupakan sumber penting berbagai enzim termasuk enzim fibrinolitik. Enzim ini diperlukan untuk mendegradasi bekuan darah pada orang yang mengalami penyakit trombosis. Isolat bakteri WU 021055* asal Pantai Papuma Jember terbukti menghasilkan enzim fibrinolitik ekstraseluler. Penelitian ini bertujuan mengetahui ukuran protein yang memiliki aktivitas fibrinolitik dan mengidentifikasi karakteristik morfologi isolat WU bakteri WU 021055*. Penelitian ini dilakukan di Laboratorium Mikrobiologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Jember pada April–Agustus 2014. Aktivitas fibrinolitik presipitat protein (PP) ditentukan menggunakan metode fibrin plate agar dan zimografi fibrin. Ekstrak protein kasar (EPK) dipanen pada jam ke-12 dan dipresipitasi menggunakan amonium sulfat 80%. Hasil uji aktivitas fibrinolitik menggunakan fibrin plate agar menunjukkan presipitat memiliki aktivitas fibrinolitik lebih besar dibanding dengan EPK. Dari hasil karakterisasi PP menggunakan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) diperoleh 11 pita protein dengan ukuran 12–41 kDa. Berdasar atas hasil zimografi fibrin, pita protein dengan berat molekul 24 kDa yang memberikan aktivitas fibrinolitik. Protein dengan ukuran 24 kDa ini mampu mendegradasi substrat fibrin. Simpulan, isolat bakteri WU 021055* mengandung berbagai protein ekstraseluler, memiliki bentuk koloni bulat berwarna putih dan termasuk bakteri gram prositif berbentuk batang.DETECTION OF FIBRINOLYTIC ACTIVITY OF WU 021055* BACTERIAL ISOLATE FROM PAPUMA BEACH COASTAL JEMBER USING ZYMOGRAPHYBacteria were important resources for various enzymes including fibrinolytic enzymes. This enzyme is capable of degrading fibrin clot in patient with thrombotic diseases. Bacterial isolate of WU 021055* from Papuma Beach Coastal Jember could secrete extracellular fibrinolytic enzymes. The objective of this reasearch was to determine the molecular weight of protein responsible for fibrinolytic activity and to identify morphologycal characterization of bacterial isolate of WU 021055*. This study was conducted at Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Universitas Jember in April–August 2014. Fibrinolytic activity of precipitate protein (PP) was determined by using fibrin plate agar and fibrin zymography. Crude protein extract (CPE) was harvested at 12 hours and precipitated by 80% ammonium sulphates. The result of fibrinolityc activity determination showed that fibrinolytic activity of PP was higher than CPE. Protein characterization of PP by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) obtained 11 different protein bands corresponds to value 12–42 kDa. Based on fibrin zymography, the 24 kDa protein might contribute to fibrinolytic activity due to degraded fibrin substrates. In conclusion, bacterial isolate of WU 021055* contained extracellular fibrin protein was white colony and gram positives bacilli able to degraded.]]>
<![CDATA[The Development Strategies on Batik Creative Industry as an Enthralling Sector for Cultural Tourism in Bondowoso Regency, East Java ]]>
<![CDATA[Syubbanul Wathon]]>;
<![CDATA[Kartika Senjarini]]>;
<![CDATA[Rike Oktarianti]]>
<![CDATA[ Warta Pengabdian Vol 13 No 2 (2019): Warta Pengabdian ]]>
Publisher : <![CDATA[LP2M Universitas Jember ]]>
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DOI: 10.19184/wrtp.v13i2.10237
<![CDATA[Bondowoso Regency has great potential to evolve into the centers of the batik creative industry. But, there are still no batik products that show the uniqueness of Bondowoso Regency. Several community groups in Suling Wetan Village, Cermee Subdistrict, Bondowoso Regency, have been running a business as batik artisans since 2015. The community groups have had the basic skill to produce batik. Unfortunately, they still don't give any impact on the growth of the cultural tourism destinations in Bondowoso. The old batik designs do not attract buyers, has limited color combinations, and only rely on synthetic batik dyes. Through the partnership service program that have been implemented, some of the Batik community business can develop their products. This partnership service program is done by several activities, such as socialization; workshops on batik design and the use of natural dyes; training on natural materials and batik coloring; procurement of gawangan machines, pendulum and accessories; training and mentoring techniques for making interesting batik motifs and coloring using natural dyes; and the dialogue to develop the creative batik industry. The results of this program are developing not only the Suling Wetan Village but also make the Batik community business in Cermee Subdistrict, independently growth as a creative batik industry. This improvement is also captivating the cultural tourism in Bondowoso Regency.]]>
<![CDATA[MORPHOLOGICAL CHARACTERISTIC DIFFERENCE BETWEEN MOSQUITOES VECTOR FOR MALARIA AND DENGUE FEVER ]]>
<![CDATA[Syubbanul - Wathon]]>;
<![CDATA[Kartika Senjarini]]>;
<![CDATA[Rike Oktarianti]]>;
<![CDATA[Ratis Nour Sholichah]]>;
<![CDATA[Ahmad Tosin]]>
<![CDATA[ BIOEDUKASI Vol 18 No 2 (2020) ]]>
Publisher : <![CDATA[PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER ]]>
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DOI: 10.19184/bioedu.v18i2.18890
<![CDATA[Malaria and Dengue Haemorrhagic Fever (DHF) are two major tropical diseases in East Java. The pathogens of these diseases are transmitted to human hosts via haematophagy by vector mosquitoes. Anopheles spp. specifically transmits Plasmodium parasites which cause Malaria, while Aedes spp. transmits Dengue viruses that cause DHF. Bangsring, Banyuwangi is one of the endemic areas of Malaria, while Jember is one of endemic areas of DHF. Two species of Malaria vectors i.e Anopheles vagus and Anopheles sundaicus have been found from several samplings which were conducted in Bangsring region. This mosquitoes have been previously identified as a major vector for malaria. Aedes aegypti and Aedes albopictus, which were major vectors for DHF, have been easily found in Jember during our sampling for any season of the year. This research wanted to differentiate the morphological characteristic of those vectors for malaria and Dengue. Adult mosquitoes can be distinguished from one another by characterizing their morphological features. Palpi, wings, and legs characteristics are commonly used as determination keys in Anopheles sp., while thoraxes and legs characteristics are used as identification keys in Aedes sp. Morphological identification is considered as the basic necessity in understanding and determining bionomic of mosquito vector. This is very important in developing effective and efficient mosquito vector control strategies, which is also an important step to prevent the death risks associated with Malaria and DHF cases.]]>
<![CDATA[THE IMMUNOGLOBULIN (IgG) ANALYSIS OF IMPLANTATION MICE (Mus musculus L.) POST OVARIECTOMY AFTER TREATMENT OF BLACK SOYBEAN FLOUR EXTRACT (Glycine soja) ]]>
<![CDATA[Yenny Febriana Ramadhan Abdi]]>;
<![CDATA[Mahriani Mahriani]]>;
<![CDATA[Kartika Senjarini]]>;
<![CDATA[Rike Oktarianti]]>
<![CDATA[ BIOEDUKASI Vol 19 No 1 (2021) ]]>
Publisher : <![CDATA[PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER ]]>
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DOI: 10.19184/bioedu.v19i1.21099
<![CDATA[Black soybean is one type of plant that has estrogenic activity, it can act as a source of natural exogenous estrogen. Decreased estrogen hormone in the body due to ovariectomy and menopause, it affect on immune response (IgG). The purpose of this study was to determine the effect of black soybean extract on immune response (IgG) of implantation mice post ovariectomy. Twenty eight mice were grouped into 4 groups, group 1 (negative control), group 2 (positive control with the administration estradiol concentration 50 ppm), group 3 and 4 were treatment group with the administration of black soybean flour extract doses of 0.31 g/ml and 0.63 g/ml. The data were analyzed using One Way Anova and Duncan Multiple Range Test (DMRT) (p<0,05). The treatment with administration of black soybean flour extract for 10 days in implantation mice post ovariectomy showed that a dose of 0.31 g/ml and dose of 0.63 g/ml had an effect on increasing significantly the immune response (IgG), i.e 0,12987and 0,12996 respectively.]]>
<![CDATA[PURIFICATION OF 31 AND 67 kDa PROTEIN FRACTION FROM SALIVARY GLAND OF Aedes Albopictus (SKUSE) (DIPTERA: CULLICIDAE) ]]>
<![CDATA[Syubbanul Wathon]]>;
<![CDATA[Rike Oktarianti]]>;
<![CDATA[Nuril Azizah]]>;
<![CDATA[Yasir Mubarok]]>;
<![CDATA[Riana Agatha Listiani]]>;
<![CDATA[Kartika Senjarini]]>
<![CDATA[ BIOEDUKASI Vol 19 No 1 (2021) ]]>
Publisher : <![CDATA[PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER ]]>
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DOI: 10.19184/bioedu.v19i1.18892
<![CDATA[Aedes albopictus mosquito is a potential vector for Dengue Haemorrhagic Fever (DHF) which transmits Dengue virus during blood feeding. The success of the blood feeding process is aided by the biological activity of proteins in the salivary glands of Aedes albopictus. There are 30 types of proteins from the salivary glands of Aedes albopictus which are carried along blood feeding process. Proteins in the salivary glands act as vasodilator and immunomodulator. Previous studies have identified two immunogenic proteins from the salivary glands of Aedes albopictus with molecular weight of 31 and 67 kDa. Further research on the biological function of these proteins requires its purified protein to better specify the target to developing a dengue vaccine. The objective of this study was to obtain 31 and 67 kDa purified proteins by implementation of electroelution and dialysis purification. The 31 and 67 kDa protein was successfully purified by this method. This has been confirmed by a single band visualization after SDS-PAGE analysis]]>
<![CDATA[PLATELET AGGREGATION IN VITRO ANALYSIS OF 67 kDa IMMUNOGENIC PROTEIN FRACTION FROM Aedes albopictus SALIVARY GLAND (SKUSE) (DIPTERA: CULICIDAE) ]]>
<![CDATA[Rike Oktarianti]]>;
<![CDATA[Syubbanul - Wathon]]>;
<![CDATA[Intan Fitri Indrasari]]>;
<![CDATA[Nadya Rismana Fitriani]]>;
<![CDATA[Kartika Senjarini]]>
<![CDATA[ BIOEDUKASI Vol 18 No 2 (2020) ]]>
Publisher : <![CDATA[PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER ]]>
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DOI: 10.19184/bioedu.v18i2.18891
<![CDATA[Cardiovascular disease is caused by impaired heart and blood vessel function. Coronary heart disease includes acute coronary syndrome due to narrowing of the coronary arteries. Aspirin is an anti-platelet drug which is commonly used for primary and secondary prevention of coronary heart disease. The effectiveness of aspirin has limitations because 10-20% of patients who use aspirin continue to experience vascular blockage. The haematophagus arthropods salivary glands contain apyrase which can inhibit platelet aggregation and thus a potential candidate for anti-platelet drug discovery. Our studies from salivary gland protein extract of Aedes albopictus found a 67 kDa protein fraction which has a similar molecular weight range from the previously identified apyrase of other mosquitoes vectors. The purpose of this study was to determine the apyrase potential capacity of 67 kDa protein fraction from salivary gland of Ae. albopictus. The present study employed inhibition percentage of platelet aggregation method in determining its apyrase activity. Aspirin was used as a positive control with 2 different concentrations of 0.1 mg/mL and 2 mg/mL. PBS 1mM pH 7.4 was used as a negative control treatment, while negative control without treatment only involved the addition of PRP and ADP. The inhibition percentage activity from the 67 kDa sample ranged from 3-37% whereas the total protein extract comprised of only 1%. The positive control of aspirin was 0.1 mg/mL and 2 mg/mL, resulting in inhibition percentage of 5% and 17%, respectively. The data showed that the inhibition percentage of platelet aggregation from protein 67 kDa is generally higher than those of total salivary gland protein extract as well as positive control. This indicated that the 67 kDa protein has a potential apyrase activity.]]>
<![CDATA[KARAKTERISASI BERBASIS MARKA MOLEKULER ITS2 TERHADAP SUB-SPESIES KOMPLEKS Anopheles vagus vagus DAN Anopheles vagus limosus ]]>
<![CDATA[Kartika Senjarini]]>;
<![CDATA[Lailly Nur Uswatul Hasanah]]>;
<![CDATA[Miatin Alvin Septianasari]]>;
<![CDATA[Muhammad Khalid Abdullah]]>;
<![CDATA[Rike Oktarianti]]>;
<![CDATA[Syubbanul Wathon]]>
<![CDATA[ Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021 ]]>
Publisher : <![CDATA[Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT) ]]>
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DOI: 10.29122/jbbi.v8i2.4737
<![CDATA[The presence of intraspecies variations of An. vagus later categorized as the subspecies of An. vagus vagus and An. vagus limosus, could be an obstacle to the identification process, which is an important step for malaria vector’s competence characterization. Based on morphological identification, those subspecies could be distinguished by the presences of pale scales in prehumeral and pale bands in proboscis. The objective of this research was to compare subspecies complexes of An. vagus morphologically and molecularly using Internal Transcribed Spacer 2 (ITS2). Anopheles samples were collected from Bangsring, Banyuwangi. Their phylogenetic tree was constructed by using NJ method based on their ITS2 sequences. BLAST result showed that An. vagus vagus and An. vagus limosus were similar to An. vagus FJ654649.1 from East Java Indonesia and East Timor based on its 99% homology and their molecular distance. The Neighbour Joining (NJ) tree grouped those subspecies in one clade with a boostrap value of 82%. This subspeciation might be due to the different rates of evolution. ITS2 sequences of An. vagus vagus and An. vagus limosus were submitted to GenBank with the accession number of MW314227.1 and MW319822.1, respectively. Kemunculan variasi intraspesies An. vagus yang kemudian dikategorikan sebagai subspesies An. vagus vagus dan An. vagus limosus menjadi kendala dalam proses identifikasi yang merupakan langkah penting dalam menentukan kompetensi vektor malaria. Berdasarkan karakter morfologi, subspesies tersebut dibedakan dengan adanya sisik pucat pada bagian prehumeral dan pita pucat pada probosis. Penelitian ini bertujuan untuk membandingkan subspesies An. vagus secara morfologis dan molekuler menggunakan Internal Transcribed Spacer 2 (ITS2). Nyamuk Anopheles didapatkan dari Bangsring, Banyuwangi. Konstruksi pohon filogeni dilakukan berdasarkan sekuen ITS2 yang dianalisis menggunakan metode NJ. Hasil BLAST menunjukkan, ITS2 An. vagus vagus dan An. vagus limosus memiliki tingkat homologi 99% dan jarak evolusi molekuler terendah dengan An. vagus FJ654649.1 dari Jawa Timur Indonesia dan Timor Timur. Pohon NJ mengelompokkan subspesies tersebut dalam satu klade dengan nilai boostrap 82%. Hal ini dapat terjadi karena perbedaan kecepatan evolusi yang memungkinkan terjadinya subspesiasi. Urutan basa ITS2 dari An. vagus vagus dan An. vagus limosus telah didaftarkan ke GenBank dengan nomor aksesi MW314227.1 dan MW319822.1.]]>
