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All Journal Caraka Tani: Journal of Sustainable Agriculture
Tri Joko
Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry

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Fruit Morphology, Antioxidant Activity, Total Phenolic and Flavonoid Contents of Salacca zalacca (Gaertner) Voss by Applications of Goat Manures and Bacillus velezensis B-27 Haviah Hafidhotul Ilmiah; Endang Sulistyaningsih; Tri Joko
Caraka Tani: Journal of Sustainable Agriculture Vol 36, No 2 (2021): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v36i2.43798

Abstract

Snake fruit (Salacca zalacca (Gaertner) Voss) is one of indigenous fruits from Southeast Asia that has been consumed for its antioxidant contents. Improving the fruit quality might increase its benefits for human health. This study aims to analyze fruit morphology, antioxidant activity, total phenolic and flavonoid contents of the fruit applied with goat manures and Bacillus velezensis B-27. The research used two-factor Randomized Complete Block Design (RCBD) with three blocks as replications. The first factor is the level of goat manure application i.e. 0 kg plant-1, 5 kg plant-1 and 10 kg plant-1, while the second factor is the bacteria application i.e. applied with Bacillus velezensis B-27 and without Bacillus velezensis B-27. The research was conducted in June until December 2019 at Turi, Sleman, Yogyakarta, Indonesia. Fruit length, diameter and shape were measured as morphology parameters. Antioxidant activities were measured using 2,2-Diphenyl-1-picrylhydrazyl, whereas flavonoid and phenolic contents were determined by the aluminium-chloride colorimetric and Folin-Ciocalteu methods, respectively. Means of each parameter were analyzed using ANOVA, continued with the Tukey’s HSD test at a 5% significance level. The result showed that the application of 10 kg goat manure per plant with bacteria increased the fruit length (76.78 mm) and the diameter (62.72 mm). Addition of 10 kg goat manure per-plant combined with Bacillus velezensis B-27 gave the highest antioxidant (IC50 of 37.83 µg.mL-1), flavonoid (5.35 mgGAE.100 g-1) and total phenolic contents (44 mgQE.100 g-1).
The Potential of Rhizophagus intraradices, Bacillus thuringiensis Bt BMKP and Silica for Anthracnose Disease Control in Shallot Rizky Amallia; Suryanti Suryanti; Tri Joko
Caraka Tani: Journal of Sustainable Agriculture Vol 38, No 2 (2023): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v38i2.76536

Abstract

Anthracnose caused by Colletotrichum gloeosporioides is a prevalent disease that poses a significant threat to shallot production in Indonesia. To mitigate this issue, the use of biological agents presents an alternative for disease control, reducing the inherent risks associated with the use of chemical pesticides. Therefore, this study aimed to determine the potentiality and mechanism of the biological agents (Rhizophagus intraradices and Bacillus thuringiensis) combined with silica. These agents were evaluated both individually and in combination to suppress the development of anthracnose in Tajuk variety shallot. The study was conducted in the laboratory and greenhouse, arranged in a completely randomized design with six treatments and three replications. The ability to suppress pathogenic fungi was determined based on in vitro antagonism tests of B. thuringiensis, disease severity, area under disease progress curve (AUDPC), plant height, number of leaves, number of tillers and the percentage of mycorrhizal fungal infections. Furthermore, the mycorrhizal infection on plant roots was observed using staining methods. The results showed that the Bt BMKP isolate was included in the B. thuringiensis strain RC9 group with the capacity to inhibit C. gloeosporioides in vitro by 18.88%. The combination treatment of R. intraradices, B. thuringiensis and silica reduced infection from anthracnose by 15.52% compared with control. These three treatments also significantly increased the agronomic performance of shallot up to six weeks after planting compared to control and other treatments.
Co-Authors Endang Sulistyaningsih Haviah Hafidhotul Ilmiah Rizky Amallia Suryanti Suryanti