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All Journal Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi BERKALA FISIKA JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Biogenesis: Jurnal Ilmiah Biologi AGRIVITA, Journal of Agricultural Science Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Life Science

Nintya Setiari

Departemen Biologi, Fakultas Sains Dan Matematika Universitas Diponegoro

Author-ID : 376641

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health

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Micropropagation of Dendrobium phalaenopsis Orchid Through Overexpression of Embryo Gene AtRKD4 Setiari, Nintya; Purwantoro, Aziz; Moeljopawiro, Sukarti; Semiarti, Endang
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.1690

To increase the efficiency of crop production from Dendrobium phalaenopsis orchids, mass propagation has been performed by inducing somatic embryogenesis through Agrobacterium-mediated transformation of the Arabidopsis embryo gene AtRKD4 into orchid protocorm (developing orchid embryo). The three-week-old protocorms of D. phalaenopsis were genetically transformed with T-DNA carrying 35S :: GAL4 :: AtRKD4 :: GR through A. tumefaciens strain EHA 105. The cultures were maintained in VW medium with 10 mg L-1 Hygromycin. Due to the existence of glucocorticoid response element (GR) in the T-DNA construct, the transformed protocorms were transferred into VW medium with the addition of 15 μM Dexamethasone in 6 weeks after transformation to activate the transgene. A total of 12% protocorms has been confirmed for Hyg + by using PCR. The expression of embryo gene AtRKD4 was confirmed by cDNA analysis using AtRKD4 specific primers and Actin primers as a positive control experiment. The expression level of AtRKD4 in 2.5-month-old D. phalaenopsis transformant shoots was 7 times higher than non-transformant plants, and increased to 86 times higher in 8-months, that much higher than that of non-transformant. These results provide an improved method for genetic transformation of D. Phalaenopsis and will (eventually) increase production efficiency in the future.

Induksi Kalus dari Hipokotil Alfalfa (medicago sativa l.) secara in vitro dengan Penambahan Benzyl Amino Purine (BAP) dan Î±-Naphtalene Acetic Acid (NAA) Hayati, Surya Kurnia; Nurchayati, Yulita -; Setiari, Nintya -
Bioma : Berkala Ilmiah Biologi Vol. 12, No. 1, Tahun 2010
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Alfalfa (Medicago sativa L.) is useful plant for treatment some diseases such as: cancer, diabetes, lupus, andhepatitis. Propagation of this plant in Indonesia face a problem which has no embryo. One method to propagate thisplant is by tissue culture or micropropagation. Callus induction is first step in micropropagation to produce calluswhich will be regenerated to become planlet. The aims of this research are to induce callogenesis from hipocotyl ofalfalfa with addition Benzyl Amino Purine (BAP) and Î± Naphtalene Acetic Acid (NAA), and to determine the propercombination of BAP and NAA to produce the optimal callus. The experiment has been conducted by using 12combination of BAP and NAA with Completely Randomized Design (CRD) in 4x3 factorial pattern by 5 replicates.Data were analyzed by ANOVA 95% Degrees of Freedom (DF). If there was significance result, it was followed byDMRT analyzed at 95 % DF. The result showed that combination of BAP and NAA was able to induce callogenesisfrom hipocotyl of alfalfa. The optimal callus was obtained in combination of BAP 0 ppm and NAA 2 ppm.

Respon Pertumbuhan dan Produksi Senyawa Antioksidan pada Kalus Rosela (Hibiscus sabdariffa L.) dari Eksplan yang Berbeda secara in vitro Noviati, Agustin; Nurchayati, Yulita; Setiari, Nintya
Bioma : Berkala Ilmiah Biologi Vol. 14, No. 2, Tahun 2012
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Callus induction method can be used as tool in producing plant secondary metabolites. One of this compound found in roselle (Hibiscus sabdariffa L.) is antioxidant agent i.e ascorbic acid and carotenoid. The callus could be induced from any kind of explants in tissue culture. The aims of this experiment is to select explant which can encourage of callus formation beside high level of antioxidant compounds. The treatment of experiment was three kinds of explants i.e section of leaf, petiole and flower sepal. Sterilized explants were planted in MS (Murashige&Skoog) combined with 2 mg/L NAA dan 5 mg/L BAP.Â This experiment has been conducted by completly randomized design with 5 replicates Growth variable, included fresh weight callus, growth presentation, callus respons and biochemical variable like content of ascorbic acid and carotenoid were analyzed.Â The data were analyzed by analysis of varian and Duncanâ€™s test at 95% significant level. Thed results showed that all kinds of explant dedifferentiated into callus which antioxidant content. Callus from leaf section had the higgest fresh weight with high level ascorbic acid. Whereas the higgest carotenoid level was obtained from callus-derived flower sepals. It conclused thatÂ in vitro callus was useful for producing plant biochemical compounds. Â Keywords : Callus induction, antioxidant agents, explants, ascorbic acid, cartenoid

Induksi Kalus Binahong (Basella rubra L.) Secara In Vitro Pada Media Murashige & Skoog Dengan Konsentrasi Sukrosa Yang Berbeda Sitorus, Ertina Novaria; Hastuti, Endah Dwi; Setiari, Nintya -
Bioma : Berkala Ilmiah Biologi Vol. 13, No. 1, Tahun 2011
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Binahong (Basella rubra L.) is a plant medicine consisting secondary metabolites which have virtue asmedicines for several diseases that could also be used as coloring agent. The medicine compounds in secondarymetabolites could be extracted from callus. Sucrose is one of the components that build MS (Murashige & Skoog)medium. Sucrose is important in in vitro culture, it functions as carbon and energy source for explant to grow. Thepurposes of this research are to study the effect of sucrose in MS medium towards B. rubra L callus formation andgrowth; to find the optimum sucrose concentration for callus B. rubra L formation; and also to find the fastestinitiation time to produce callus crumb. This research uses Complete Random Design (CRD) single factor method,i.e., sucrose concentration of 0 g/l, 10g/l, 20 g/l, 30 g/l, 40 g/l with five repetitions. The data is analysed withAnalysis of Varian (Anova) and if a real difference is found the analysis is continued with Duncan Multiple RangeTest (DMRT) with significancy level of 95%. The results show that various sucrose concentrations in MS mediuminfluences callus B. rubra L induction. The highest sucrose concentration, i.e. 40 g/l, which was added into MSmedium, could induce the maximum callus wet-weight of 1,69 g and the fastest callus initiation time of 4,8 day.

Eksplorasi Kandungan Klorofil pada beberapa Sayuran Hijau sebagai Alternatif Bahan Dasar Makanan Tambahan Setiari, Nintya -; Nurchayati, Yulita -
Bioma : Berkala Ilmiah Biologi Vol. 11, No. 1, Tahun 2009
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Chlorophyll is a plant pigment which recently have been popular as food supplement. The source ofchlorophyll consumed now are alfalfaâ€™s leaves algae such as Spirulina and Chlorella.. Chlorophyll content find in allkinds of plant especially vegetables so that have potent to be source of chlorophyll. The aims of this research are toexplore alternative source of chlorophyll aside of Leguminosae, from vegetables such as sweet basil, spinach, waterspinach, cassava leaves, pegagan, and papayaâ€™s leaves and to study the vegetables potent to be food supplement.The experiment has been conducted by extracting 0,1 g each leaves in 10 mL acetone 85%. The extract was filteredwith Whatman paper No. 1, thus record the absorbance by using spektrofotometer UV Vis. Statistical analysis wasused to analyze chlorophyll content is Analysis of Variance (ANOVA) with Randomized Complete Design (RCD) in95% significancy. If the ANOVA showed significantly different, the analysis were continued with Duncanâ€™s test.The result showed that the highest content of chlorophyll-a, b, and total found in papayaâ€™s leaves. However thelowest one was found in sweet basil. Papayaâ€™s leave potential as food supplement.

Kajian Fisis Radiasi Plasma Terhadap Organ Daun pada Pertumbuhan Awal Tanaman Anggrek Phalaenopsis amabilis [Physics Observation of Plasma Radiation for Leaves to Initial Growth of Phalaenopsis amabilis] Nur, Muhammad; Setiari, Nintya; Azam, Much.; Selawanti, Ika Indah
BERKALA FISIKA Vol 10, No 1 (2007): Berkala Fisika
Publisher : BERKALA FISIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.062 KB)

The Phalaenopsis amabilis (â€anggrekâ€) has been irradiated by nitrogen ions for growth Â accelereting.Irradiation technique used corona plasma technology that its electrodes configuration was point-to-plan geometry to generate nitrogen ions in air. Corona glow discharge Plasma has been generated by DC Voltage of 3.50 kV and current was 0,23 mA.Â Nitrogem ions that were irradiated to Phalaenopsis amabilis (â€anggrekâ€) have been bombarded toÂ leaf organ during 1 toÂ 7 minutes. We used two types Phalaenopsis amabilis control:Â control with fertilizer and control without fertilizer. Growing of Phalaenopsis amabilisÂ can be accellereted about 90 % compare with control withoutÂ fertilizer and 30 % compare with control with fertilzer. The maximum growing of Phalaenopsis amabilis by using plasma technology for time irradiation of 1 or 2 minutes. Â Key wordsÂ : corona glow discharge plasma, Phalaenopsis amabilis (anggrek), nitrogen, ion electrons,Â free radicals.

Produksi dan Konsumsi Oksigen serta Pertumbuhan Ceratophyllum demersum L. pada Kerapatan yang Berbeda dalam Mendukung Potensinya sebagai Bioaerator Hidayat, Muhammad Khusni; Izzati, Munifatul; Setiari, Nintya
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 19, No 2 (2011): Volume XIX, Nomor 2, Oktober 2011
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Aquaculture is one of important economic activity in Indonesia. The main problem in aquaculture is the low water quality such lower oxygen level . Ceratophyllum demersum is one of aquatic plants that is hypothesized capable in increasing oxygen level through photosynthesis. However the growth of C. demersum it self will consume oxygen from the waterÂ through respiration. The aim of this study is to measure oxygen production and consumption by C. demersum at different density level. From this data, we will understand the potency ofÂ C.demersum as bioaerator to supplay oxygen in the water. This experiment was designed using Completed Randomized Designed (CRD). Three density level of C. demersum was apllied as treatment. They were 100g/100L, 200g/100L dan 300g/100L. Each treatment was replicated by 4 times. Results indicated that in density of 300g/100L produced the highest oxygen level ( 1,65 ppm). The highest growth rate of C. demersum was resulted by density level of 200g/100L.

PERTUMBUHAN TUNAS LATERAL TANAMAN NILAM (Pogostemon cablin Benth) SETELAH DILAKUKAN PEMANGKASAN PUCUK PADA RUAS YANG BERBEDA Irawati, Heny; Setiari, Nintya
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 17, No 2 (2009): Volume XVII, Nomor 2, Oktober 2009
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Patchouly is one of the plants that produce essential oil, i.e. patchouly oil, which is commonlÂ used in cosmetic industry. Increasing the biomass of patchouly plants by triggering the growth could be one way to increase the production of pathouly oil. Therefore, the purpose of this research is to study the effect of apical bud cutting towards patchouly growth, and also to understand the optimum location of cutting on the nodus. The research used Complete Random Design method with 5 repetitions. There were 3 locations of cutting (R), i.e. R0 (without cutting), R1 (cutting at the first nodus), R2 (cutting at the second nodus). The observed variables are length and amount of lateral bud, wet weight, and dried weight. The data were then analysed using ANOVA with significancy level of 95%. If there were real different, the analysis were then continued using Duncan test with significancy level of 95%. The results showed that apical bud cutting gives real effect to the variable of length and amount of lateral bud. The untreated plant has the most lateral bud shorter than the treated ones. For the variables of wet and dried weight there were not any significant differents. Generally, cutting at the first nodus (R1) of patchouli plant gives better result than any other treatments.

Pengaruh Pemberian Vitamin C (asam askorbat) terhadap Kesegaran Bunga Krisan (Chrysanthemum sp) pada Kawasan Sentra Penghasil di Desa Ngasem, Kecamatan Jetis, Bandungan, Jawa Tengah Arisanti, Desi; Setiari, Nintya
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 12, No 1 (2012): VOL XX, NOMOR 1, TAHUN 2012
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Bunga Krisan (Chrysanthemum sp) merupakan salah satu jenis bunga potong yang popular dan banyak diminati oleh konsumen karena bentuk dan warnanya yang menarik serta ukuran yang bervariasi. Budidaya bunga krisan di Desa Ngasem merupakan mata pencaharian vital bagi petani karena sebagian besar bertanam bunga krisan. Permasalahan dalam budidaya bunga krisan potong yaitu penanganan pascapanen. Bunga yang dipotong, lama kesegarannya berkurang seiring menurunnya kandungan unsur hara dalam tanaman. Petani di Desa Ngasem mempertahankan kesegaran bunga potong krisan hanya direndam dalam air. Penelitian ini bertujuan untuk mengetahui cara penanganan pascapanen bunga potong krisan di Desa Ngasem dan mengetahui pengaruh pemberian vitamin C dalam larutan perendam (pulsing) sehingga dapat memperpanjang lama kesegaran bunga potong krisan. Perlakuan dalam percobaan adalah perbedaan komposisi larutan perendam bunga potong krisan, yaitu perlakuan P0 : larutan perendam berupa air 500 ml; perlakuan P1 : larutan perendam berupa campuran air 500 mlÂ danÂ vitaminÂ C 100 ppm; perlakuan P2 : larutan perendam berupa campuan air 500 ml dan Â vitamin Â C 200 ppm. Masing-masing perlakuan diulang sebanyak 3 kali dengan rancangan percobaan berupa Rancangan Acak Lengkap (RAL) dengan pola faktor tunggal.. Parameter yang diamati yaitu lama kesegaran bunga potong krisan. Berdasarkan uji F hasil pengamatan menunjukkan bahwa lama kesegaran bunga potong krisan tidak dipengaruhi oleh pemberian vitamin C dalam larutan perendam tetapi hanya dipengaruhi oleh air tanpa pemberian vitamin C. Perlakuan air sebagai kontrol (perlakuan P0) mampu mempertahankan lama kesegaran bunga potong krisan tertinggi, yaitu 14 hari. Perlakuan dengan pemberian vitamin C (P1 dan P2) hanya mampu mempertahankan lama kesegaran bunga potong krisan selama 12 hari.

Kapasitas Penyerapan dan Penyimpanan Air pada Berbagai Ukuran Gel dari Tepung Karaginan untuk Pembuatan Media Tanam Jeloponik Hakim, Muhammad Faisol; Setiari, Nintya; Izzati, Munifatul
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 17, No 1 (2009): Volume XVII, Nomor 1, Maret 2009
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

There is a trend in using gel for hydrophonic. We examine the potency of water absortion and holding capacity from different size of carrageenan gel. This research was conducted from may to july 2006. the size of gel, i.e.: 1 cm3, 2 cm3, 3 cm3 and in powder form were used as independent variables. Where as, water absorbtion and holding capacity act as dependent variables. Collected data were analized by ANOVA. Result indicated that gel size significantly effect water absorbtion and holding capacity. In powder form, water absorbtion and holding capacity is the most optimal, but the gel was easily destroyed. Therefore, the best size if gel that should be used for hydrophonic were 2 cm3 and 3 cm3.

Co-Authors Ade Arisma Fauziah Ade Arisma Fauziah Agustin Noviati Aziz Purwantoro Desi Arisanti Diah Nurmaningrum Endah Dwi Hastuti Endang Saptiningsih Endang Semiarti Erma Prihastanti Ertina Novaria Sitorus Eva Septiyana Fakhri Fadhlurrohman Pratama Farida Nur Hasanah Fauziah, Ade Arisma Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Heny Irawati Ika Indah Selawanti Ika Susanti Hendriyani Jefri Saputro Luaeliyah, Masrukhatul Much. Azam Muhammad Faisol Hakim Muhammad Khusni Hidayat Muhammad Nur Munifatul Izzati NIDA, KHOIRIN Nita Kumalasari Dewi Nurullita Prahasti Prahasti, Nurullita Sri Darmanti Sri Widodo Agung Suedy Sukarti Moeljopawiro Sulistyowati Sulistyowati Surya Kurnia Hayati Tanti Dwi Romawati Ulva, Maria Wahyu Hadayani Yulita Nurchayati

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