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PENGARUH TRANSPLANTASI ALLOGRAF PANCREATIC STEM CELL TERHADAP KADAR INSULIN DAN C-PEPTIDE TIKUS PUTIH PENDERITA DIABETES MELITUS TIPE I Setiawan, Boedi; Plumeriastuti, Hani
Majalah Kedokteran Bandung Vol 48, No 3 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (541.957 KB) | DOI: 10.15395/mkb.v48n3.842

Abstract

Penyakit diabetes melitus merupakan salah satu penyakit degeneratif yang hingga kini masih belum tuntas terapinya dan masih menjadi ancaman serius bagi dunia kesehatan di Indonesia dan dunia. Tujuan penelitian ini adalah mengetahui kadar insulin dan C-peptide tikus putih penderita diabetes melitus tipe I yang diberikan transplantasi allograf pancreatic stem cell dengan laparotomi intrapankreatik. Penelitian ini dilakukan selama 6 bulan (Juli?Desember 2014) di lab. Stem Cell, Institute of Tropical Diseases, Universitas Airlangga, Surabaya. Dua belas tikus putih jantan Rattus novergicus galur Wistar dibagi secara acak menjadi dua kelompok . Kelompok pertama (P0) disuntik aloksan 150 mg/kg bobot badan tanpa terapi stem cell . Kelompok kedua disuntik aloksan dengan dosis 150 mg/kg bobot badan dan diterapi dengan 1x106/kg bobot badan stem cell pankreas secara laparotomi intrapankreatik (P1). Akhir penelitian adalah pada hari ke-31 percobaan. Hasil penelitian menunjukkan bahwa kadar glukosa darah pada akhir penelitian berbeda sangat nyata (p <0,01) antara kelompok perlakuan yang menerima terapi stem cell (P1) dengan P0 kontrol positif, meskipun nilai kadar glukosa darah rata-rata tidak senormal seperti pada hari ke-1. Tingkat C-peptide dan insulin P0 dan P1 berbeda sangat nyata (p<0,01). Dapat disimpulkan bahwa terapi stem cell secara laparotomi intrapankreatik dapat menurunkan kadar glukosa darah, serta meningkatkan kadar C-peptide dan insulin. [MKB. 2016;48(3):135?39]Kata kunci: Diabetes melitus, insulin, stem cell Insulin and C-peptide Levels in Diabetes Mellitus Type I White Rats  treated with  Pancreatic Stem Cell Allograft TransplantationDiabetes mellitus is one of the degenerative diseases in which the therapy still remains unresolved and is still a serious threat to the global health, including to the health of Indonesian people. The aim of this study was to describe the level of insulin and C-peptide in diabetes mellitus type I white rats treated with  pancreatic stem cell allograft through intrapancreatic laparotomy. This study was conducted at the Institute of Tropical Diseases, Universitas Airlangga, Surabaya in a 6 month period (July?December 2014). Twelve male white rats Rattus novergicus Wistar strain, were randomly divided into two groups. The first group (P0) was injected by alloxan, 150 mg/kg body weight, without stem cell therapy. Another group was injected by alloxan, 150 mg/kg body weight, and was treated with 1x106/kg body weight pancreatic stem cell throughintrapancreatic laparotomy (P1). The experiment was finalized on the 31th day of the experiment. The results showed that the blood glucose levels at the end of experiment were highly significantly different p<0.01 between the treatment group that received stem cell therapy (P1) and P0 positive control, although the average value of blood glucose levels was not as normal as on the first day. C-peptide and insulin levels of P0 and P1 group differed significantly (p<0.01). It can be concluded that stem cell therapy through intrapancreatic laparotomy can reduce blood glucose levels and increase the levels of C-peptide and insulin. [MKB. 2016;48(3):135?39]Key words: Diabetes mellitus, insulin, stem cell
Identification of Acanthocephala on Water Monitor Lizard (Varanus salvator) Human Consumed in Sidoarjo Ahmad Herdianto Wicaksono; Emmanuel Djoko Poetranto; Tatik Hernawati; Boedi Setiawan
Journal of Parasite Science (JoPS) Vol. 4 No. 2 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v4i2.29159

Abstract

Water monitor lizards are consumed by humans for its meat. The demand this reptile consumption because the taste and belief of the people in healing disease. Based on habitat and food, they have the potential to transmit zoonosis and have a high possibility of being infested by parasites. One of the possible disease that could infect water monitor and transmitted to human is acanthocephaliasis, infection from Acanthocephalan. Which previous research found Acanthocephala on intestine of frog. This research aims to know and identify Acanthochepala worms that infect water monitor lizards. Based on the results, 40 samples were used and there are 2 positive samples with Acanthocephalan in the body cavity.
Prevalence of Gastrointestinal Parasites on Beef Cattle in Jombang District Melda Mufidatul Ifqiyyah; Boedi Setiawan; Agus Wijaya
Journal of Parasite Science (JoPS) Vol. 5 No. 2 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v5i2.30367

Abstract

The disease that is quite detrimental to cattle is gastrointestinal parasitic infection. Gastrointestinal parasites infect the cattle by taking host nutrients that would caused emaciation, lethargy, and decreased productivity. This study aims to determine the prevalence of gastrointestinal parasites in cattle in Jombang District. This research was conducted in July to August 2019 with 100 samples of cattle feces. Sample examinations were carried out in the laboratory of the Division of Veterinary Parasitology, Universitas Airlangga using the sedimentation and floating methods. Detection of parasites in all samples using microscope with magnification of 100x and 400x. On examination, several types of parasites were found included Strongyloides papillosus, Toxocara vitulorum, Oesophagostomum spp., Moniezia expansa, Blastocystis spp. and Eimeria spp. The results of this study were found the prevalence of gastrointestinal parasites on beef cattle in Jombang district was 35% with a worm infection prevalence of 15% and protozoan infections of 20%.
Perbandingan Kadar Elektrolit Serum Pascaenterektomi Ekstensif 75 % pada Anjing yang Diterapi dengan Laktoferin Boedi Setiawan; Sudarminto -; Hartiningsih -
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

A severe and extensive intestine damages can occur in dogs suffering from volvulus , strangulation,intersuception and neoplasia. Extensive enterectomy is the most common medical treatment for dogssuffering from such disorder. A study was therefore conducted to compare the serum electrolyte level ofextensively enterectomyzed dogs after being treated with different level (0%, 0.05% and 0.5 mg/bodyweight) of lactoferin. As many as 9 dogs at 3-4 moth-old and with the body weights ranging from 4-5 kgwere used. After being enterectomized to the extent of 75% out of the total length of the intestine, the dogswere divided randomly into 3 groups (I, II and III) each of which consisted of 3 dogs. The three groups weretreated for 30 days respectively with 0.0 mg/body weight, 0.05mg/kg body weigth and 0.5mg/kg bodyweight of lactoferin. The electrolyte levels (Na, K and Cl ion) of dogs were determined at days 1, 15 and 30of during the treatment. The data collected from this study were analysed by Analysys of Variance (Anova)proceded by Duncan Multiple Range test (DMRT). The result showed that at days 15 and 30 of thetreatment, the electrolyte levels of the dogs with lactoferin (0.05 and 0.5 mg/body weight) were significantlyhigher than in dogs without lactoferin (0mg/bodyweight). The level of Na ion of dogs with 0,5, 0,05, dan 0mg/kg bw lactoferin were 143, 143,4, dan 141,7 mEq/L respectively at day 15 and 147, 150, dan 137,7mEq/L respectively at day 30. The levels of K ion for those dogs were 5,17, 4,97, dan 3,83 mEq/Lrespectively at day 15 and 30: 5,1, 5,13, 3,73 mEq/L respectively at day 30. Meanwhile, their Cl levelswere 113,7, 114,3, 104 mEq/L respectively at day 15 and 115,3, 117,3, dan 91,3 mEq/L respectively atday 30. Hasil penelitian ini menunjukkan bahwa memperian lactoferin memperbaiki kadar elektrolitserum anjing yang dienterektomi secara ekstensif. It was evidence that lactoferin treatment can improvedthe electrolyte profiles of extensively enterotomized dogs.
Identification of ectoparasites and endoparasites on fruit bats (Cynopterus brachyotis) in Ketapang Timur Village, Ketapang Sub-District, Sampang District Mohamad Safri Sauqi; Tjuk Imam Restiadi; Setiawan Koesdarto; Poedji Hastutiek; Boedi Setiawan; Agus Wijaya
Journal of Parasite Science (JoPS) Vol. 5 No. 2 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v5i2.30365

Abstract

This study aims to determine the ectoparasites and endoparasites that infest fruit bats (Cynopterus brachyotis) in Ketapang Timur, Ketapang District, Sampang Regency. The samples in this study were blood, feces and ectoparasites from 50 fruit bats taken in Ketapang District, Sampang Regency. Blood samples were examined using a blood smear method with Giemsa staining, while stool samples were examined using three methods, namely native, sucrose floating, and acid-fast modification. Blood examination was checked using a microscope with 1000x magnification and stool examination at 400x magnification. The sample is considered positive if under microscope observation found parasites that match the characteristics of the protozoa sourced from scientific references. The results of the study of 50 fruit bats examined found five positives for digestive protozoa and ectoparasites, the infection was single, with details of one tail being infected with Eimeria sp. and one tail was infected by Leptocyclopodia ferrarii, while blood protozoa were not found. This study concludes that the type of protozoa found in the digestive tract is Eimeria sp. (14%) and ectoparasite Leptocyclopodia ferrarii. (2%). The total percentage obtained was 16% positive for a single infection of protozoa and ectoparasites from 50 fruit bats. Suggestions that can be put forward are to conduct further research using PCR and sequencing to obtain more specific and accurate identification results, for subspecies or strains.
Relationship between the value of the estrous detector measurement result and serum progesterone level in Etawa crossbred goats after estrous synchronization Annisa Lita Triagil; Ismudiono Ismudiono; Tita Damayanti Lestari; Abdul Samik; Budi Utomo; Boedi Setiawan
Ovozoa : Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.82-85

Abstract

This study was conducted to determine the relationship between the estrous detector's reading value (Draminski®, Dramiński S.A, Poland) and serum progesterone levels on Etawa crossbred does. Eighteen healthynon-prehnant Etawa crossbreds does weighing 20-25 kg, in their first parity were used in this study. The does were divided equally into two groups. In the two groups, the does were synchronized their estrus by means of intramuscular and intravulvar injection of 7.5 mg prostaglandin Fɑ (PGF2ɑ) twice in 11-day intervals. After the second injection, the estrous detector value measurement, visual observation of the estrus sign, and blood sampling were conducted for five days. The blood sample was processed into the serum to examine for progesterone levels by ELISA. The data analysis was using the simple correlation method on SPSS type 23 for windows. The result showed that a positive relationship (p <0.05) with coefficient correlation (r) was 0.609 between the values of the estrous detector measurement with serum progesterone levels. It could be concluded that the estrous detector's low value indicates that the Etawa crossbred was in the estrus phase and followed by the decreasing of progesterone level but not reaching the basal level.