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ISOLASI BERBAGAI MIKROBA PADA RHIZOSFIR TANAMAN JAGUNG ., Nurmayulis; Setiawati, Mieke Rochimi; Utama, Putra; ., Susiyanti
Jurnal Agroekoteknologi Vol 1, No 2 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.242 KB) | DOI: 10.33512/j.agrtek.v1i2.596

Abstract

ABSTRACT Biodiversity of potential  soil microbes has been regarded as biological fertilizer of Zea mays in marginal land. A few microbe increased the rate of growth  of plant throught absorbtion slow released N and P in soil. The isolation, identification and population of soil microbes from rizosphere at Walantaka (Banten province) had been done in the Soil Biology Laboratories and the greenhouse, Pajajaran University- Bandung, during Mei to Agustus 2009. The soil was collected randomly from sites in Walantaka. To increase N and P using trapping and selection medium from from rizosphere at Walantaka (centre of Zea mays production), province of Banten.  The Exploration of potential microbe Isolates of Azotobacter, Azospirillum, microbes dissolver phosphate (MPF) dan Mycorrhyzae microbes were identified and were founded in soil samples. The result showed, on okon selection medium  can be found 4 Azospirillum, on Ashby medium  lived 4 Azotobacter,  and Pikovskaya medium got 2 MPF and Mycorrhyzae microbes.Key words: marginal land, Azospirillum, Azotobacter, MPF, Mycorrhyzae, Zea mays
Potency of tea plant indigenous microbe on plant growth and to against blister blight disease (Exobasidium vexans Massee) Fauziah, Fani; Setiawati, Mieke Rochimi; Susilowati, Dwi Ningsih; Pranoto, Eko; Rachmiati, Yati
Jurnal Penelitian Teh dan Kina Vol 19, No 1 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.257 KB) | DOI: 10.22302/pptk.jur.jptk.v19i1.77

Abstract

The chemical control method of blister blight (Exobasidium vexans Massee) on tea could inflict various negative impacts. In order to obtain an environmentally sound control method of blister blight disease, a nursery trial has been conducted to know the effectiveness of bacterial combinations. The trial was carried out at Gambung experimental garden, with seven treatments and four replications. The indigenous microbial codes are Azoto II-1, Endo-5 and Endo-76. The treatments tested comprised: control (without bacteria),  Azoto II-1 25% + Endo-5 75%; Azoto II-1 50% + Endo-5 50%; Azoto II-1 75% + Endo-5 25%;  Azoto II-1 25% + Endo-76 75%; Azoto II-1 50% + Endo-76 50%; and Azoto II-1 75% + Endo-76 25%. All of the treatments was applied as a soil drench, 50 ml/plant with bacterial concentration at 0,5%. The parameter observed was blister blight disease intensity, plant heights, stem diameter, leaves number, root length, and root volume. The results showed that the combination of Azoto II-1 75% + Endo-5 25% could suppress the intensity of blister blight disease with disease intensity 1.27%. The treatments also affected plant heights, stem diameter, leaves number, root length, and root volume, 15.32 cm; 3.38 cm; 8.05 cm; 18.25 cm and 2.37 cm, respectively.
The comparison of effectivity from consortium bio fertilizer and endophytic bio fertilizer on productivity and health of clone mature GMB 7 tea crop Setiawati, Mieke Rochimi; Wulansari, Restu; Pranoto, Eko
Jurnal Penelitian Teh dan Kina Vol 17, No 2 (2014)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.884 KB) | DOI: 10.22302/pptk.jur.jptk.v17i2.48

Abstract

This study aimed at comparing the effec­tiveness of consortium bio-fertilizer with endo­phytic bio fertilizer to increasing produc­tivity and health of mature clone GMB 7 tea crop. This research was carried out at the Gambung Experimental Station Block A7 Research Insti­tute for Tea and Cinchona, from July 2013 to September 2013. Randomized block design was used in this study with ten treatments and three replications i.e.: A1H0 = anorganic fertilizer (75%), A2H0 = anorganic fertilizer (50%), A1H1 = anorganic fertilizer (75%) + 2 liter consortium bio fertilizer, A1H2 = anorganic fertilizer (75 %) + 4 liter consor­tium bio-ferti­lizer, A2H1 = anorganic fertilizer (50 %) + 2 liter consortium bio fertilizer, A2H2 = anorga­nic fertilizer (50%) + 4 liter consor­tium bio-fertilizer, A1H3 = anorganic fertilizer (75 %) + 2 liter endophytic bio fertilizer, A1H4 = anor­ganic fertilizer (75%) + 4 liter endo­phytic bio fertilizer, A2H3 = anorganic fertili­zer (50%) + 2 liter endophytic bio fertilizer and A2H4 = anorganic fertilizers (50%) +4 liter endophytic bio fertilizer. The results showed there were a significantly difference on amount of the pekoe shoot among treatments, although the others variable was not significantly diffe­rent. However, the overall consortium bio-fertilizer resulted in the production of shoots 15,36% and endophytic biofertilizer application resulted in the production of shoots 21,93% higher than the control (without bio fertilizer). When compared to the first plucking, the num­ber of shoot from July to September showed increasing trend, so did the number of banji shoots.
In-vitro experiment of nitrogen fixing capacity of indigenous and exogeneous Azotobacter sp at Andisol tea planting area’s Pranoto, Eko; Setiawati, Mieke Rochimi
Jurnal Penelitian Teh dan Kina Vol 17, No 1 (2014)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.597 KB) | DOI: 10.22302/pptk.jur.jptk.v17i1.43

Abstract

An in-vitro experiment to evaluate total nitrogen content of soil of tea plantation area included with indigenous and exogenous Azoto­bacter sp have been conducted in the Biology and Soil Biotechnology Laboratory, Padja­djaran University. A completely randomized design with 11 treatments and three repli­cations was used. The treatments were: A (ne­gative control); B (positive control); C (A. Kedelai II); D (A. vinelandii); E (A. N.D.9.3); F (A. Padi); G (VI-1); H (II-1); I (V-2); J (I-1); and K (III-2). Size of microbe inoculated popu­lation for each treatment was 1,2 x 1011 CFU/ml. One percent dose was used for ste­rilized soil originated from the tea plantation area. After five days of incubation period re­vealed that II-1 Azotobacter sp produced very significantly different in total nitrogen pro­duction than the other Azotobacter sp, i.e. 0,8532%. Orthogonal comparisons indicated that indigenous Azotobacter sp had the capacity of nitrogen fixation 7,67% higher than that the capacity of nitrogen fixation of exogenous Azotobacter sp for growth on Andisol soil of tea plantation area with pH of 4,5-5,6. Although, Azotobacter sp is very rarely found in soil with low pH (high acidity), this phenomena indi­cating that an active biofertilizer of indigenous Azotobacter sp is present in tea plantation area.
Comparison of some exogeneous solubillizing phosphate bacteria on Andisol as Indonesia dominant native tea soil Setiawati, Mieke Rochimi; Pranoto, Eko
Jurnal Penelitian Teh dan Kina Vol 18, No 2 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.23 KB) | DOI: 10.22302/pptk.jur.jptk.v18i2.78

Abstract

Tea was planting an upland area with Andisol as the dominant soil ordo. Andisol has a special characteristic i.e. low soil pH and it has more mineral that causing low of soil phos-phate soluble, especially for tea plants, there-fore the rule of solubilizing phosphate bacteria will be needed. Laboratory scale experiments were conducted to study the capability activities of some solubilizing phosphate bacterias name-ly Pseudomonas cepaceae, P. malei, Bacillus mycoides, and B. subtilis. The experiment was arranged in Completely Randomized Design with six treatments and four replications. The result on statistical analysis shown that there were no significant differences in increasing of the soil phosphate soluble. Nevertheless, the treatment D (Bacillus mycoides) produced the highest soil phosphate soluble. The capability of bacteria to increase soil phosphate soluble was depended on its habitat, especially soil pH. Even all of the isolates were the superior solubilizing phosphate bacterias in agricultural food plants with neutral soil pH, but they have not significantly different on Andisol. There-fore, the using of indigenous isolates on tea plant areas was suggested.
PENGARUH NaCl DAN AMELIORAN ORGANIK Azolla pinnata TERHADAP N-TOTAL, pH TANAH, SERTA BOBOT KERING TANAMAN PADI (Oryza sativa L.) PADA INCEPTISOLS JATINANGOR Khoiriyyah, Luluatul; Setiawati, Mieke Rochimi; Suriadikusumah, Abraham
Jurnal Agroekoteknologi Vol 9, No 1 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.242 KB)

Abstract

The high content of NaCl in soil may lead to nutrient imbalance and inhibit the Nitrogen uptake by plants. Azolla pinnata can be used as an organic ameliorant to help plant roots in absorbing nutrients and enhancing the number of available N for plants. The aims of this research were to ascertain whether Azolla pinnata as organic ameliorant gave the significant difference on the enhancement of N content of soil, pH of soil, and dry weight of rice plant on various salinity level of soil and also to find out if Azolla pinnata can suppress salinity stress at certain salinity. The experiment was conducted at the Greenhouse of Faculty of Agriculture Universitas Padjadjaran, Jatinangor and also at the Laboratory of Soil Fertility and Plant Nutrion, Departement of Soil Science and Land Resources on November 2016 until March 2017. The experimental design used was randomized block design with 8 treatments and 4 replications. The treatments consist of without the organic ameliorant Azolla pinnata, and with the application of Azolla pinnata on salinity level 0, 2, 4, 6 mmhos cm-1. The results showed that the application of NaCl and Azolla pinnata gave the significant difference on soil pH, and dry weight of plant. The application of Azolla pinnata is not able yet to suppress the rice symptom of salinity stress at 2, 4, and 6 mmhos cm-1 on the enhancement of dry weight of plant. The application of Azolla at salinity 6 mmhos cm-1 was equivalent with salinity 2 mmhos cm-1 in producing dry weight of plant.
APLIKASI PUPUK HAYATI BAKTERI ENDOFITIK TERHADAP POPULASI BAKTERI ENDOFITIK, SERAPAN N DAN BOBOT KERING TANAMAN PADI (Oryza Sativa L.) PADA TANAH SALIN Utami, Deswita; Setiawati, Mieke Rochimi
Jurnal Agroekoteknologi Vol 9, No 1 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.242 KB)

Abstract

Productivity of rice crops (Oryza sativa) which grown in saline soils can improve due to application endophytic bacteria biofertilizer. Endophytic bacteria lives in plant tissues have an able to fix nitrogen from the air that for supply N to plant tissue more optimal. The research aims was to find the effects of endophytic bacteria biofertilizer on enhance bacterial populations, nitrogen uptake, and dry weight of rice grown in saline soil. This research was conducted from March 2016 to February 2017 in Soil Biology Laboratory and in Laboratory of Soil Chemistry and Fertility, Soil Science Department and Land Resources, Faculty of Agriculture, Universitas Padjadjaran and Experiment Field of Ciparanje, Faculty of Agriculture, Universitas Padjadjaran with 752 meters above the sea level. This research used randomized block design experiment which consist of eight combinations: A (salinity of 0 dS m-1), B (salinity of 0 dS m-1 + biofertilizer), C (salinity of 2 dS m-1), D (salinity of 2 dS m-1 + biofertilizer), E (salinity of 4 dS m-1), F (salinity of 4 dS m-1 + biofertilizer), G (salinity of 6 dS m-1), H (salinity of 6 dS m-1 + biofertilizer). The result showed that at salinity 2 dS m-1 rice plant still survive growing on salinity stress. Salinity treatment of 2 dS m-1 + endophytic biofertilizer have enhance affect to bacterial population, N uptake and dry weight of rice compared to salinity of 4 and 6 dS m-1
PENGARUH PUPUK ORGANIK (Azolla pinnata) TERHADAP C-ORGANIK TANAH, SERAPAN N DAN BOBOT KERING TANAMAN PADI (Oryza sativa L.) PADA TANAH DENGAN TINGKAT SALINITAS TINGGI Arafah, Mayang Sunduz; Setiawati, Mieke Rochimi; Nurbaity, Anne
Jurnal Agroekoteknologi Vol 9, No 1 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.242 KB)

Abstract

Utilizing organic fertilizer Azolla pinnata is needed especially for rice commodity which planted in salin soil in coast area for increasing the efficiency of inorganic fertilizer by adding organic matters. This research aimed to get the best combination of utilizing organic fertilizer Azolla pinnata to increase total organic carbon, nitrogen uptake, and dry weight of rice in saline soil. This research used Randomized Complete Block Design experiment which consisted of eight combinations of treatment. Those are treatment of salinity 0, 2, 4 and 6 mmhos cm-1 + without utilizing organic fertilizer Azolla pinnata and treatment of salinity 0, 2, 4, and 6 mmhos cm-1 + utilizing organic fertilizer Azolla pinnata. The result showed that utilizing organic fertilizer Azolla pinnata can reduce the negative effect from salinity which proven by increased N uptake of rice in treatment of salinity 6 mmhos cm-1.
PENGARUH BAKTERI ENDOFITIK DAN Azolla pinnata TERHADAP POPULASI BAKTERI ENDOFITIK, KANDUNGAN N, DAN BOBOT KERING P PADI (Oryza sativa L.) PADA TANAH BERSALINITAS Melani, Melani; Saribun, Daud Siliwangi; Setiawati, Mieke Rochimi
Jurnal Agroekoteknologi Vol 9, No 1 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.242 KB)

Abstract

Nitrogen deficiency is the main problem of rice growth in salt stress condition. Endophytic bacteria has an ability to enhance the number of available N in plant tissue by fixing N from the atmosphere, meanwhile Azolla pinnata has high content of N that can increase the available N in soil. The aim of this research was to know the limit of salinity stress tolerance in rice with application of endophytic bacteria and A. pinnata on enhancing the population of endophytic bacteria, N content, and dry weight of rice on soil with salinity. The research was conducted on March 2016 until March 2017 at the Green House of Ciparanje Universitas Padjadjaran. Samples were analized at the Laboratory of Soil Biology and Laboratory of Soil Fertility and Plant Nutrion, Faculty of Agriculture, Universitas Padjadjaran. The design experiment used was single factor randomized block of 8 treatments and 4 repetitions. The treatments consist of with and without the application of endophytic bacteria and A. pinnata on soil with salinity of 0, 2, 4, and 6 mmhos cm-1. The results showed that the application of endophytic bacteria and A. pinnata gave the significant difference on the population of endophytic bacteria, N content, and dry weight of rice up to 4 mmhos cm-1.
Preferensi petani terhadap keragaan padi (Oryza sativa) unggul untuk lahan sawah di wilayah Pangandaran dan Cilacap Prayoga, Muhamad Khais; Rostini, Neni; Setiawati, Mieke Rochimi; Simarmata, Tualar; Stoeber, Silke; Adinata, Kustiwa
Kultivasi Vol 17, No 1 (2018)
Publisher : Fakultas Pertanian UNPAD

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Abstract

Abstract The object that conducted in this study is farmers preferences of rice plant charac-teristics. This study was taken in 3 villages which are village Ciganjeng, village Pamotan, and village Rawaapu. The method used in this study was quantitative design with descriptive analysis. The respondents were 64 rice plant farmers. Measuring instrument used was questionnaire and interview. The result of this study showed that farmers has its own preference for the character of rice plants. Conclution of this study is farmers preferred rice plants which has an average height of plants, wide leaves, leaves surface, leaves angle, and rice straws type; rice straw that has many branches; strong braches; has a lot rice grains; maturity of rice plants; lenght and wide grains size; non-aromatic; and tender texture. The farmers thought that leaves surface isnt an important character of a rice plants;  high of plants, leaves lenght and wide, leaves angle, rice straws type, grains lenght and wide, and rices scent are quite important characteristics; branchs strenght, grains quantity, and plants maturity are an important characteristics; resistance to pests, disease resistance, drought resistance, resistance to inundation, salinity resistance, productivity, and rice texture are the most important characteristics. Keywords : preferences, farmer, superior varietiesObjek yang dikaji dalam penilitian ini adalah preferensi petani terhadap karakter tanaman padi. Tempat penelitian dilaksanakan di 3 desa (Desa Ciganjeng, Desa Pamotan dan Desa Rawaapu). Desain penelitian menggunakan desain kuantitatif dengan pendekatan analisis data secara deskriptif. Metode yang diperunakan adalah survey untuk mengumpulkan data dengan mengedarkan kuisioner dan melakukan wawancara. Jumlah petani yang diwawancarai yaitu 64 orang. Hasil dari penelitian ini adalah petani lebih menyukai tanaman padi yang memiliki tinggi tanaman, panjang daun, lebar daun, permukaan daun, sudut daun dan tioe malai yang sedang, cabang malai sekunder yang banyak, ketegaran batang yang kuat, jumlah anakan yang banyak, umur yang genjah, ukuran gabah yang panjang dan lebar, aroma yang tidak wangi, serta tekstur nasi yang pulen. Petani beranggapan bahwa karakter permukaan daun merupakan karakter yang tidak penting; karakter tinggi tanaman, panjang daun, lebar daun, sudut daun, tipe malai, panjnag gabah, lebar gabah, dan aroma tergolong karakter cukup penting; karakter ketegaran batang, jumlah anakan, dan umur tanaman merupakan karekter yang penting; karakter ketahanan terhadap hama, ketahanan terhadap penyakit, ketahanan terhadap kekeringan, ketahanan terhadap genangan, ketahanan terhadap salinitas, produktivitas, dan karakter tekstur nasi merupakan karakter yang sangat penting.Kata kunci : preferensi, petani, varietas unggul