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Perkembangan Dan Ukuran Pertama Kali Matang Gonad (Lm50) Ikan Lemadang (Coryphaena hippurus Linnaeus, 1758) Betina Di Perairan Selatan Bali Hety Hartaty; Iriani Setyawati; FX. Sudaryanto
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p02

Research on the reproductive biology of fish can provide information on spawning frequency, spawning success, spawning time, and size at first maturity. This research aimed to determine the reproductive biology of common dolphinfish (Coryphaena hippurus Linnaeus, 1758) landed at the fish landing site (PPI) Kedonganan, Badung, Bali. Sampling was conducted in May - September 2018. A total of 42 female common dolphinfish with length ranged between 41-110 cm FL were obtained from hand-line fishery based on FADs. Ovaries were prepared using the paraffin method and Harris Haematoxylin-Eosin staining for histological observation. The results showed that the gonad maturity level in adult females was: spawning (38.10%), spawning capable (35.71%), regressing-potentially reproductive (4.76%), regressed 1 (2.38%), regressed 2 (2.38%), and regenerating (2.38%). Immature female: immature (9.52%) and developing (4.76%). Oocyte diameter ranged between 43,20 – 864,66 µm with an average of 430,99 µm. Fecundity in fish size 49-75 cm FL ranged from 30,720-433,080 eggs / fish with an average of 129,594 eggs / fish. The difference in the level of oocytes in the ovaries at the same time showed that female common dolphinfish in the southern Bali waters has an asynchronous ovary development and indeterminant fecundity with multiple spawning strategies and size at first maturity (Lm50) was 43.2 cm FL. Keywords: common dolphinfish, gonad histology, size at maturity

PENURUNAN FUNGSI HATI TIKUS BETINA (Rattus norvegivus L) YANG DIINJEKSI WHITE VITAMIN C DOSIS TINGGI DALAM JANGKA WAKTU LAMA DITINJAU DARI KADAR SGPT, SGOT SERTA GAMBARAN HISTOLOGI HATI Ni Wayan Sudatri; Iriani Setyawati; Ni Made Suartini; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p07

This study aims to determine the side effects of high-dose injections of vitamin C in a long term against liver histology. This study used a completely randomized design (CRD) with different long treatments of vitamin C injection in various doses of P0 (control), P1 (30 days injection), P2 (50 days injection), P3 (70 days injection) and P4 (90 days injection). Parameters measured were plasma levels of SGPT and SGOT, the percentage of hydropic degeneration, fatty degeneration, pyknotik nuclei, and necrosis of liver cells, and also inflammatory cell infiltration and sinusoid congestion. SGOT levels showed a significant differences (P=0,009) between the treatment and control group by ANOVA test. The Kruskal Wallis test showed that ALT levels were not significantly different (P=0,86), however, the levels of creatinine showed significant differences (P=0,002). The hydropic degeneration were not significantly different (P=0,054) by Kruskal Wallis test, whereas the fatty degeneration were significantly different (P=0,000) between the treatment and control groups by ANOVA test. Pyknotik nuclei and necrotic cells showed significant differences between the control and treatment with the P value of 0,016 and 0,01 respetively. The injected white vitamin C in high doses for long periods decreased the physiological functions of female mice liver.

Efektivitas Ekstrak Hipofisa Ayam Broiler (Gallus sp.) Terhadap Kinerja Reproduksi Dan Kelulushidupan Larva Ikan Lele Sangkuriang (Clarias sp.) Adevalentin Lesik; Iriani setyawati; NI Gusti Ayu Manik Ermayanti
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p05

The pituitary gland of broiler chickens (Gallus sp.) is empirically known to have gonadotropins to accelerate ovulation in fish. This research was conducted to determine the reproductive performance and survival of sangkuriang catfish (Clarias sp.) Larvae of adult male and female mature gonads by 20 pairs. This study used a Completely Randomized Design consisting of 4 treatments and 5 replications to obtain 20 experimental units. Doses of pituitary extract used is without injection of pituitary chicken pituitary extract (P0), injection of pituitary chicken pituitary extract with a dose of 600 mg/kgbb (P1), 900 mg/kgbb (P2) and 1200 mg/kgbb sangkuriang catfish (P3). Intramuscular injection of extracts in the ventral dorsal fin with a volume of 0.5 mL/kgbb. Descriptive data analysis for gonad maturity level, ovulation response, egg performance. Statistical analysis of data for morphometric aspects using the Wilcoxon test, for latency time, relative fecundity, fertilization success, hatching success, and larval survival aspects using One Way Anova using SPSS version 25. The results of statistical analysis showed significant differences in reproductive performance (relative latency and fecundity time), successful fertilization and successful hatching of eggs, but there was no significant difference in survival (number of live larvae, length and weight) larvae of sangkuriang catfish (Clarias sp.). Broiler chicken pituitary extract (Gallus sp.) can improve reproductive performance, fertilization and hatching success but does not affect egg performance and survival of sangkuriang catfish (Clarias sp.) larvae. Keyword: catfish, pituitary extract, reproduction, survival

Histopatologi serta Aktivitas Hati Kelinci Lokal (Lepus sp.) yang Diberi Ransum Tepung Daun Kaliandra (Calliandra calothyrsus Meissn.) dan Kulit Nanas (Ananas comosus L.) I Gede Wiranatha; Iriani Setyawati; Ngurah Intan Wiratmini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p07

The aimed of this study was to determine the effect of feed containing various concentrations of Calliandra calothyrsus Meissn. leaf meal mixed with pineapple peels and concentrate feed on histopathology and the activity of liver of local rabbit (Lepus sp). This study used a Post-Test Only Control Group Design, using 24 local male rabbits divided into 4 groups of feed treatment namely P0 = concentrate feed as control; P1 = mixture of 15% of calliandra leaf meal (CLM), 30% of pineapple peels (PP) and 55% of commercial feed (CF); P2 = 30% of CLM, 30% of PP and 40% of CF; and P3 = 45% of CLM, 30% of PP and 25% of CF). The treatments were given for 60 days and used 6 replicates for each group. The data were analyzed statistically by SPSS program version 22. The research results showed the increase of calliandra leaf meal levels in concentrated feed which were added 30% of pineapple peels still caused liver damage namely fatty degeneration, necrosis and congestion. It increased the ALT level, but did not affect the AST level in local rabbit serum.

Hepatoprotektor Rumput Laut Eucheuma cottonii Pada Tikus Putih (Rattus norvegicus L.) Jantan Yang Diinduksi Natrium Nitrit (Nano2) Ida Ayu Ratih Purnama Dewi; Ngurah Intan Wiratmini; Iriani Setyawati
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p12

Sodium nitrite (NaNO2) is a food preservative that is often added to processed meat products. Excessive consumption of NaNO2 can be detrimental to health. One effort to minimize the negative effects caused by NaNO2 is by consuming antioxidants. This study aimed to determine the effectiveness of Eucheuma cotonii seaweed extract on improving blood profile, liver and kidney function in male white rats (Rattus norvegicus) induced by NaNO2. This research used a Completely Randomized Design (CRD) consisting of two control groups and three treatment groups. Each group consists of six replications. Negative control (K-) receive aquades and CMC NA 0,5%. Positive control (K +) was only received 22.5 mg/kgBW NaNO2 for 35 days then on the day 36 until the day 45 only given 0,5% CMC Na. The treatment groups P1, P2, and P3 respectively on day 1 to day 15, only received 22.5 mg/kgBW NaNO2 in the morning, and then day 16 to day 35 received 22.5 mg/kgBW NaNO2 in the morning and received 150 mg/kgBW (P1), 300 mg/kgBW (P2), 450 mg/kgBW (P3) Eucheuma cottoni seaweed extract in the evening. Day 30 to day 45, the treatment groups only received Eucheuma cottoni seaweed extracts at each dose. Blood and liver were collected on day 46. Data analysis of serum alanine transaminase (ALT) and aspartate aminotransferase (AST) enzyme levels and liver histology were performed with Anova statistical test and continued with Duncan post hoc test. The results showed Eucheuma cottonii extract dose of 150 mg / kgBB significantly reduced the level of liver damage seen from the levels of ALT and AST and liver histology (percentage inflammatory cell infiltration, congestion, fatty degeneration and necrosis) in white rats induced by sodium nitrite (NaNO2). Keywords: Preservative, AST, ALT, histopathology, liver

Penampilan Reproduksi dan Perkembangan Skeleton Fetus Mencit Setelah Pemberian Ekstrak Buah Nanas Muda Iriani Setyawati
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.764 KB)

This experiment was performed to examine the effects of young pineapple (Ananas comosus) fruitextract on reproduction performance and foetus skeletal development given during organogenesis period.Twenty pregnant mice were randomly divided into four groups. The young pineapple fruit extract wastreated orally by gavage with doses 0% (control), 20%, 40%, and 80% since gestation day 6 to 15. Caesareansection were performed to pregnant mice on day 18 to remove foetuses. Observation covered : reproductionperformance (uterine weight before and after foetus removal), the foetus number (reabsorptions, dead/alive foetus), morphological of the foetus (weight and length of the litter, and malformations), and skeletaldevelopment (ossification number of metacarpus, metatarsus, vertebrae caudalis) and malformations ofcostae, sternebrae, and vertebrae. Statistical analysis was performed using Anova and Duncan’s MultipleRange Test.Teratogenic effects caused decreasing of earlier uterine weight and alive foetus, increasingdead foetus and hemorrhage, delayed skeletal ossification (decreasing ossification number of metacarpusand metatarsus) and caused costal malformation (intercostal fusion and convulated costal structure).Young pineapple fruit extract given to pregnant mice during organogenesis alter reproductive performancepregnant mice. The extract also caused morphological abnormalities (cretinism), hemorrhage, delayedossification on metacarpus and metatarsus, and costae malformation of the foetus.

Histologi Tubulus Seminiferus dan Kadar Testosteron Tikus yang Diberi Pakan Imbuhan Tepung Daun Kaliandra dan Kulit Nanas (HISTOLOGY OF SEMINIFEROUS TUBULES AND TESTOSTERONE LEVEL OF RAT GIVEN CALLIANDRA LEAF MEAL AND PINEAPPLE PEELS IN THE DIETS) Iriani Setyawati; I Gusti Ngurah Agung Dewantara Putra; Ni Gusti Ketut Roni
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Calliandra calothyrsus leaf contains 17-28% protein which is hihly potential for use as source of protein supplement for animal feed. However, the plants also contain high level (>10%) of condensed tannins as antinutritional effects which may reduce consumtion efficiency of diet. The addition of protease (bromelain) into feed containing tannin is expected to decrease the negative effects of tannins. Bromelain can be obtained from the pineapple plant (Ananas comosus) including on the peels. This study was conducted to determine the effect of calliandra tannin combined with bromelain protease of pineapple peels in the feed on testicular histology and testosterone level of rats feeded during the growth period. The feeding experiment on post-weaning male rats was conducted using a completely randomized factorial design (4 main factors x 4 subfactors). The main factors were calliandra leaf meal substitution of 0; 10; 17.5 and 25% in the diets and subfactors were addition of pineapple peels, 0; 4.35; 8.70 and 13.05 g/rat/day. Rats were divided into 16 groups and were feeded the diets for two months (during the growth period). The results showed the susbtituion of feed with calliandra leaf had no sigbnificant effecton the thickness of the seminiferous tubules, but it appeared to have significant effect on the histology of seminiferous tubules. Increased pineapple peels level in the diets containing calliandra decreased wall thickness of the seminiferous tubules of the rat testes, indicating that bromalein fastened the maturation of spermatozoa. The addition of pineapple peels into the diet containing calliandra had a significant interaction effect on testoteron levels of male rats, but the testoteron levels among all treated rats were still within the normal range. ABSTRAK Daun kaliandra (Calliandra calothyrsus) mengandung protein 17-28% sehingga potensial sebagai sumber protein pakan ternak, namun tanaman ini mengandung condensed tannin cukup tinggi (>10%) yang bersifat antinutrisi. Penambahan enzim protease (bromelin) pada pakan yang mengandung tanin diharapkan dapat mengatasi dampak negatif tanin. Bromelin dapat diperoleh dari tanaman nanas (Ananas comosus) termasuk dari kulit buahnya. Penelitian ini bertujuan untuk mengetahui pengaruh tanin daun kaliandra dalam pakan yang dikombinasikan dengan protease bromelin dari limbah kulit nanas terhadap histologi testis dan kadar hormon testosteron tikus jika diberikan pada masa pertumbuhan. Penelitian ini merupakan percobaan pakan pada tikus jantan pascasapih selama masa pertumbuhan dengan Rancangan Acak Lengkap pola faktorial berjenjang 4x4 dengan level substitusi tepung daun kaliandra 0; 10; 17,5 dan 25% dalam ransum (main factor) dan dosis aditif kulit nanas 0; 4,35; 8,70 dan 13,05 g/ekor/hari (sub factor). Tikus dibagi menjadi 16 unit percobaan dan diberi perlakuan ransum selama dua bulan (masa pertumbuhan). Hasil penelitian menunjukkan bahwa tebal dinding tubulus seminiferus testis tikus tidak berbeda nyata, namun tampak adanya perbedaan pada gambaran histologi yang meliputi struktur membran basal, susunan antar sel-sel spermatogenik pada dinding tubulus, serta gambaran sel-sel spermatozoa di dalam lumen tubulus. Peningkatan dosis kulit nanas dalam ransum yang mengandung kaliandra menurunkan tebal dinding tubulus seminiferus. Aditif kulit nanas ke dalam ransum yang mengandung kaliandra menunjukkan interaksi yang memengaruhi kadar hormon reproduksi dengan menurunkan kadar hormon testosteron tikus jantan walaupun masih dalam kisaran normal.

MORFOLOGI FETUS MENCIT (MUS MUSCULUS L.) SETELAH PEMBERIAN EKSTRAK DAUN SAMBILOTO (ANDROGRAPHIS PANICULATA NEES) IRIANI SETYAWATI
Jurnal Biologi Udayana Vol 13 No 2 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.026 KB)

This experiment was performed to examine the effects of Andrographis paniculata Nees leaves extract on foetus morphology if given during organogenesis period. Thirty pregnant mice were randomly divided into 5 groups for teratogenic effects. Extract was given orally by gavage with 0 (as control); 0,004 (equal to one time dose to human); 7,5; 15; and 22,5 g/g body weight/ day. Treatment was given from day 6 to day 13 of gestation (organogenesis period). Foetus were removed on day 18 of gestation by caesarean section. Quantitative data were analyzed with Anova and Duncan's Multiple Range Test. The results showed that teratogenic effects increased in dose-related manner by causing foetus death and resorption, hemorrhage and clubfoot.

PERTUMBUHAN, HISTOPATOLOGI OVARIUM DAN FEKUNDITAS IKAN NILA MERAH (OREOCHROMIS NILOTICUS) SETELAH PAPARAN PESTISIDA ORGANOFOSFAT IRIANI SETYAWATI; NGURAH INTAN WIRATMINI; JOKO WIRYATNO
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Fish cultures in rice fields or areas that use water from a pesticide-polluted irrigation or river, are particularly vulnerable to the negative impact of pesticides. This study aims to assess the effect of organophosphates on growth, ovarian histopathology and fecundity of red tilapia (Oreochromis niloticus). After acclimatization, 160 fishes (fingerlink stage) were kept in four ponds sized 2x1,5x0,8 m each. This study used a complete randomized block design and organophosphate administration through the feed. The study used four groups: control, and variations in dose of 2 ml (D1), 6 ml (D2), and 10 ml (D3) of pesticide per 100 grams of pellet, which is given in the morning (treatment) and in the evening (regular feed). Data were taken at week 3, 6, and 9, then the organophosphate treatments were stopped, and the last data were taken at week-18. Statistical analysis was performed by Anova and Duncan's Multiple Range Test. The results showed that the organophosphate pesticide inhibits the growth by reducing fish length and weight, reducing ovarian weight, increasing the number of oocyte atresia, damaging structure of the ovarian histology, and decreasing fecundity by reducing the number of egg production in the ovarian of red tilapia.

TINGGI DAN BERAT BADAN SISWA UMUR 10-12 TAHUN DI SEKOLAH PEDESAAN DAN KOTA : SD DI DESA PINANG SEBATANG TIMUR DAN KOTA PEKANBARU PROVINSI RIAU Fita Efi Rafelina Siallagan; I Ketut Junitha; Iriani Setyawati
SIMBIOSIS Vol 10 No 1 (2022)
Publisher : Jurusan Biologi Universitas Udayana

ABSTRACT The difference in height and weight is influenced by genetic traits and environmental factors. The study population was primary school children from one elementary school in the area of Pinang Sebatang Timur Village, Tualang Regency and one elementary school in urban areas covering Pekanbaru City. The two research areas are located in the province of Riau. The research sample was 60 students from Nusantara elementary school in Pinang Sebatang Timur Village and 60 students from Santa Maria elementary school, which consisted of (30 boys and 30 girls). The age range of the probandous is about 10-12 years old. The method that is being used for the research is purposive sampling method using online survey. The parameters studied were height, weight, age, parental education, parental occupation, parental income, body mass index (BMI), age of manarche and first wet dreams. Body Mass Index (BMI) was calculated by dividing body weight (kg) by height square (m2).According to the Center for Disease Control and Prevention, BMI is categorized based on 4 categories, namely underweight (<18), normal (18-25), overweight (25-27), and obese (>27). The average of heights, weights and body mass indexes of male and female students in Pekanbaru city are higher than in the Pinang Sebatang Timur’s village. Factors that are affecting student’s heights and weights are their parent’s job, their parent’s allowances, heights, the age of menarche on the female children or wet dream on the male children, and also the physical activities the students do. Keywords: Growing pattern, height, weight, age, Body Mass Index (BMI)

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