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The Effect of GnRH on Reproductive Performance and Progesterone Hormone Levels in Buffalo in West Sumatera Tinda Afriani; Ferdinal Rahim; Mangku Mundana; Arif Rahmat; Jaswandi; Anna Farhana
JURNAL TRITON Vol 11 No 2 (2020): JURNAL TRITON
Publisher : Politeknik Pembangunan Pertanian Manokwari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47687/jt.v11i2.110

Abstract

The administration of hormone GnRH will affect the reproductive organs and can improve the reproductive performance of buffalo. The decrease of the buffalo population is caused by various factors including genetic and environmental factors. The development of buffaloes is relatively slow because the reproduction rate of buffaloes is lower than that of cattle, besides that it is also difficult to detect estrus, the pregnancy period is relatively long and longer calving rate. This study aims to determine the best dose of GnRH on the swamp buffalo producing of dadih in Indonesia. The materials used were 16 swamp buffalo. A completely randomized design was used in this study. Buffalo is synchronized with the injection of 5 ml of GnRH hormone n on the first and 11th day, on the 10th to 12th day all donor buffaloes are injected with GnRH. Four different doses of GnRH (200, 225, 250 and 275 ml/buffalo) and each treatment was replicated five times. The speed of estrus and estrus length were the measured variables. The results showed that the doses of GnRH significantly (P<0.05) increase of estrus speed and estrus length. Progesterone levels have increased significantly starting from before treatment until after treatment with GnRH. It is concluded that the best GnRH doses for estrus synchronization in the swamp buffalo producing of dadih was 2. 25 ml/buffalo with estrus speed 18.2 h and estrus length 18 h.
Identification of Single Nucleotide Polymorphism (SNP) in Early Exon 10 of Follicle Stimulating Hormone Receptor (FSHR) Gene in Pesisir Cattle Tinda Afriani; Endang Purwati; James Hellyward; Jaswandi Jaswandi; Mangku Mundana; Anna Farhana; Adisti Rastosari
JURNAL ILMIAH PETERNAKAN TERPADU Vol 10, No 3 (2022)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v10i3.p264-276

Abstract

The purpose of this study was to identify genetic diversity in the early exon 10 of the FSHR gene or follicle stimulating hormone receptor in Pesisir cattle. The blood samples used were 70 blood samples from female Pesisir cattle aged 2-5 years which were obtained from BPTU-HPT Padang Mengatas, West Sumatera. The isolated DNA was then amplified using a pair of primers L and R with PCR technique which produced a fragment with a length of 847 bp. The services of 1st Base Singapore are used for sequencing the amplified product. The results of the research on the identification of the early exon 10 FSHR gene in Pesisir cattle were found that there were 5 SNPs at positions --53T>C, +17A>G, +650C>T, +706A>C and +707 ins>A in some introns 9 to exon 10 first part. The results showed that the genotypic frequency of the Pesisir cattle population was in Hardy-Weinberg imbalance and polymorphisms were found in the early exon 10 FSHR gene diversity.