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Bacteria are microbes which have an ability to live wherever there is a life. Some of the bacteria are saprophyte and some are parasitic. But most of the bacteria have not been identified or cultured; therefore the benefits are still unknown. Methanogenic bacteria are one of the saprophyte bacteria. These bacteria produces methane, a biogas as an alternative fuel in the future. Most of methanogenic bacteria are uncultured, however a few of them are found in the sewage of coconut water. The o Ardi Kapahang; Maria Bintang; D.D. Sastraatmadja; Dedy Duryadi Solichin
Forum Pasca Sarjana Vol. 30 No. 1 (2007): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Bacteria are microbes which have an ability to live wherever there is a life. Some of the bacteria are saprophyte and some are parasitic. But most of the bacteria have not been identified or cultured; therefore the benefits are still unknown. Methanogenic bacteria are one of the saprophyte bacteria. These bacteria produces methane, a biogas as an alternative fuel in the future. Most of methanogenic bacteria are uncultured, however a few of them are found in the sewage of coconut water. The objectives of this research are to isolate, characterize and identify the methanogenic bacteria that lived in coconut water. The method of this research was fermentation, analysis characterization, and identification of methanogenic bacteria. First, methanogenic bacteria were isolated from coconut water by fermentation. The samples were from four places in Minahasa, which are Rasi (I), Koka (II), Amurang (III), and Lola (IV) and one place in Bogor (V). Secondly, the methane produced from fermentation was analysed by gas chromatography and the bacteria can be characterized by Bergey’s method. The next step is the identification which was conducted by isolating the DNA, amplifying the DNA by PCR, then sequencing the DNA with BioEdit Sequence Aligment. As the result, high and stable methane was produced in Rasi (I) and Amurang (III). The characteristic of the bacteria are red colony (M) and white colony (P). The shape of the colonies is circles, gram positive, basil shape, mesofile, positive of catalase and citrate, positive of sugar fermentation, gelatin, casein and starch hydrolysis also lived in Nutrient Broth with pH 5.7-6.8. The sequencing of isolate P resulting in nucleotide composition of G 31.25%, C 20.58%, A 27.11% and T 21.04% while isolate M are G 31.34%, C 20.31%, A 27.02% and T 21.32%. The identification of isolate M is equal with Clostridium tyrobutyricum (100 %) and isolate P is very close with Clostridium tyrobutyricum (99 %). Key words: bacteria, methane, coconut water

α-Glucosidase Inhibitor Activity of some Indonesian Syzygium Extracts Emma J. Pongoh; Rymond J Rumampuk; Astin Lukum; Ardi Kapahang
Jambura Journal of Chemistry Vol 3, No 2 (2021): August
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34312/jambchem.v3i2.11416

The leaves and fruits of Indonesian Syzygium spp such as white pakoba, red pakoba and bombongan have been extracted by ethanol. The ethanolic extract was partitioned with polar solvent gradient using n-hexane, ethyl acetate and n-butanol repeatedly. Each fraction was tested for antidiabetic activity and exhibited varying degrees of antidiabetic activity when tested with α-glucosidase method. Antidiabetic test indicated that the ethyl acetate and butanol fractions of the leaves of pakoba white, red pakoba and bombongan has potential as a source of active compounds.

Penghambatan Oksidasi Lipid Minyak Ikan Cakalang (Katsuwonus pelamis) Oleh Air Jahe (Zingiber officinale var. rubrum) Selama Penyimpanan Dingin Ilevena Rolima Josef; Ardi Kapahang; Dokri Gumolung
Jurnal Kimia Fullerene Vol 4 No 2 (2019): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Fish is one of the main foods for humans that are rich of proteins, fatty, and minerals that are good for health. Unsaturated fatty acids are very easily oxidized by the presence of catalysts such as oxygen, heat, light, and the presence of metals. Ginger contains antioxidants which can inhibit oxidation of fat or oil. This study aims to inhibit fat oxidation of skipjack fish with the addition of ginger. Skipjack fish soaked in ginger water with a concentration of 10%, 20%, 30% and the control, then saved for 0, 2, 4 days. The fish was extracted by the wet rendering method and then analyzed with 2 parameters, peroxide value and free fatty acids with the titration method. The best concentration in inhibiting lipid oxidation of skipjack fish is at a concentration of 10% and 30%. The peroxide value of cakalang oils is still below the IFOS standard of 3.75 meq O2/kg and the percentage of free fatty acids is still below the standard of 1.5%

Karakterisasi Asap Cair Hasil Pirolisis Sampah Styrofoam Dengan Katalis Zeolite Olke Wisje Lumingkewas; I Dewe Ketut Anom; Ardi Kapahang
Jurnal Kimia Fullerene Vol 6 No 2 (2021): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v6i2.351

The purpose this achieved in this research is 1.To get a few percent of liguid smoke products acquired from the pyrolysis of Styrofoam waste. 2.To know the effect of using zeolite in the waste pyrolysis process styrofoam to the speed of formation of liquid smoke product .3. To characterize the physical and chemical properties of liquid smoke from pyrolysis of syrofoam wast. The research method used to convert Styrofoam waste into liquid smoke is the pyrolisis method. For characterize of liquid smoke from pyrolysis using equipment: a set of pyrolysis tools, Fenske Viscometer Oswald, Picnometer, Boilling Point Meter and a set of KG-SM alloy. The results showed that the pyrolysis of 100 grams of Styrofoam wa ste using zeolite catalyst produced an average of 69,33% liquid smoke whereas the pyrolysis of Styrofoam waste without using a catalyst produces average 61,67 % liquid smoke. Time and temperature of pyrolysis have the same influence, the higher value, the more liquid smoke is produced. the length of ime for pyrolysis of styrofoam waste uing zeolite catalyst laste for 4,5 hours, while the pyrolysis of Styrofoam without a catalyst laste for 3,5 hours. Based of the physical ang chemical properties of liquid smoke, the pyrolysis of Styrofoam waste using a catalyst or without using a catalyst can both be classified into gasoline.

Pengaruh Proses Pengeringan Tradisional terhadap Komposisi Proksimat Nike Ikan Payangkah (Ophieleotris aporos) dari Danau Tondano Sofia Satriani Krisen; Ardi Kapahang
Jurnal Kimia Fullerene Vol 4 No 2 (2019): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

The research on effect of traditional drying process againt proximate composition of the payangkah fish nike has been done. A number of payangkah fish nike samples has been taken from the Tondano lake, divided into two parts, i,e, fresh samples and other parts were traditionally dried at temperature range of 37°C for 24 hours. The proximate composition of wet and dried samples respectively specified was water content of 83.157%, protein 13.128%, lipid 1.48%, ash 2.033%, crude fiber 0.65% and carbohydrate 0.2%. While for dry samples the water content of 9.59%, protein 67.434%, ash 5.065% and crude fiber 0.64%. The results shows that traditional drying process has positive influence against some parameters on proximate composition of the payangkah fish nike.

Penggunaan Media Video dan Powerpoint Pada Mata Pelajaran Kimia Materi Larutan Penyangga Dalam Pembelajaran Daring Ainurridha Musafir; Ardi Kapahang; Jenny Kumajas
Oxygenius Journal Of Chemistry Education Vol 3 No 2 (2021): Oxygenius: Journal of Chemistry Education
Publisher : Chemistry Department, Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/ojce.v3i2.297

The purpose of this research to see is there are differences in student chemistry learning outcomes on buffer solution material on the use of video media with PowerPoint in online learning. The design used in this research is Pretest-Posttest Control Group Design. This research was conducted at SMA N 1 Kotamobagu with class XI MIA A as experimental class 1 (video media) and class XI MIA B (media PowerPoint) as experimental class 2. Normality testing using the Liliefors test showed that the data for both classes were normally distributed, and homogeneity testing using the similarity test of variance showed both classes were homogeneous. Hypothesis testing using two-way Independent Sample T-test with acceptance criteria H0 is -ttable<tcount<+ttable. It is obtained that tcount = -2.09 and ttable = 1.68 which means tcount is in the rejection region of H0, so hypothesis H0 is rejected and H1 is accepted. Therefore, it can be concluded that there are differences in student learning outcomes when using video media using PowerPoint media.

Efektivitas Strategi REACT Terhadap Hasil belajar Siswa Pada Materi Larutan Penyangga Di Kelas XI SMA Negeri 2 Langowan Zurisaday Taidi; Ardi Kapahang; Meiske Naomi Mamuaja
Oxygenius Journal Of Chemistry Education Vol 1 No 2 (2019): Oxygenius : Journal of Chemistry Education
Publisher : Chemistry Department, Universitas Negeri Manado

Chemistry is a science that studies materials that are generally abstract in nature so many students find it difficult to understand the concept of chemistry which causes a lack of interaction between real life and chemical knowledge. This study aims to determine the effectiveness of the REACT strategy in improving student learning outcomes in buffering material at SMA Negeri 2 Langowan. The research method used is Quasi Experiment Design with research design is Posttest-only Control Group Design. The population is all students of class XI Science in SMA Negeri 2 Langowan even semester 2017/2018. The sample of this study were all students of class XI IPA 1, amounting to 25 people as the experimental class and all students of class XI IPA 2, amounting to 21 people as the control class. Data collected is processed from the results of Posttest. Data were analyzed using the Independent t-test formula to test the research hypotheses and obtained tcount> ttable (7.99> 2.02), so it can be concluded that the Contextual Teaching and Learning (CTL) learning method with REACT strategy proved to be effective against improving student learning outcomes in buffer material in class XI IPA of SMA Negeri 2 Langowan.

Penerapan Metode POGIL Pada Materi Konsep Mol Di Kelas X IPA SMA Negeri 2 Langowan Gloria Viarosa Manampiring; Ignatius Santoso; Ardi Kapahang
Oxygenius Journal Of Chemistry Education Vol 1 No 2 (2019): Oxygenius : Journal of Chemistry Education
Publisher : Chemistry Department, Universitas Negeri Manado

The purpose of this study was to determine the application of the POGIL method to the learning outcomes of Grade X Science students on the mole concept material.This research was implemented in class X IPA 1 and X IPA 2 SMA Negeri 2 Langowan school year 2018/2019. The method used is experimental method with post – test only control design research. In this study applied different methods on 2 classes namely POGIL class and lecture class with the number of students of each class of 23 students. Student learning Data is derived from the post-test value. Data analysis uses test-T with a significant level of α = 0.05. Analysis of student learning statistics test results obtained the value T > T table or 4.31 > 1.68. So that the H1 can be concluded accepted and H0 rejected, this shows that students ' learning outcomes with the application of the POGIL method are greater than the student learning outcomes by the lecture method on the concept of Mole material.

Peningkatan Hasil Belajar Siswa dengan Model Course Review Horay Metode Problem Posing pada Materi Larutan Elektrolit dan Nonelektrolit di Kelas X MIPA SMA Negeri 1 Tomohon Maria Pangkey; Meytij Rampe; Ardi Kapahang
Oxygenius: Journal Of Chemistry Education Vol 4 No 2 (2022): Oxygenius : Journal of Chemistry Education
Publisher : Chemistry Department, Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/ojce.v4i2.445

This study aims to improve student learning outcomes on electrolyte and nonelectrolyte solutions through the application of the course review horay cooperative learning model with the problem posing method. The course review horay model of the problem posing method is a model that can enable students to search and find answers to a problem and the learning process also becomes more interesting. This research is classroom action research (CAR) which is conducted in 2 cycles, where each cycle consists of planning, action, observation, and reflection stages. The research subjects were all students of class X MIPA 1 at SMA Negeri 1 Tomohon, which consisted of 28 students consisting of 18 female students and 10 male students. Data collection techniques were carried out through observation and tests. Cycle I, the percentage of students' mastery classically is 78.57%. Cycle II the percentage of classical completeness to 100%. There was an increase in individual learning outcomes, with 22 students who finished studying in the first cycle to 28 students who finished learning in the second cycle. The results showed that the course review horay learning model with the problem posing method on electrolyte and nonelectrolyte solutions improved student learning outcomes.

Studi Senyawa Metabolit Sekunder Dari Fraksi Etil Asetat Daun Pare Hutan (Momordica balsamina L.) claudia christy senduk; Rymond Rumampuk; Ardi Kapahang; Emma Pongoh
Jurnal Kimia Fullerene Vol 8 No 2 (2023): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v8i2.562

Daun pare hutan (Momordica balsamina L) merupakan tanaman asli wilayah yang beriklim tropis yang tumbuh liar dan dimanfaatkan oleh masyarakat sebagai obat herbal. Penelitian ini dilakukan untuk mempelajari, senyawa metabolit sekunder yang terkandung dalam fraksi etil asetat daun pare hutan (momordica balsamina L.), dengan cara di isolasi dan di identifikasi. Proses isolasi dilakukan menggunakan metode maserasi, untuk pemisahan dan pemurnian senyawa menggunakan metode Kromatografi Lapis Tipis (KLT) dan Kromatografi Kolom Gravitasi sehingga diperoleh isolat (F1.6.1) dengan berat sampel 1,4 mg. Identifikasi senyawa mengunakan Nuclear Magnetic Resonance Proton (1H-NMR) dan Nuclear Magnetic Resonance Carbon (13C-NMR). Hasil identifikasi isolat ditentukan sebagai suatu senyawa carbinol dengan usulan nama struktur 2,2,3,3-tetramethylcyclohexane-1,1-diol dengan rumus molekul C10H18O2.

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