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Evaluation of Plant Growth Promoting Rhizobacteria as a Protecting Agent Against Cucumber Mosaic Virus and Chilli Veinal Mottle Virus on Chillipepper MUHAMMAD TAUFIK; SRI HENDRASTUTI HIDAYAT; GEDE SUASTIKA; SIENTJE MANDANG SUMARAW; SRIANI SUJIPRIHATI
HAYATI Journal of Biosciences Vol. 12 No. 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.974 KB) | DOI: 10.4308/hjb.12.4.139

Abstract

This study was conducted to evaluate the effectiveness of plant growth promoting rhizobacteria (PGPR) in protecting chillipepper plant from infection of cucumber mosaic virus (CMV) and chilli veinal mottle virus (ChiVMV). Seven isolates of PGPR, i.e. BC1, BTP2H, BTP3G, BTP3O BTP1, BTP2D, and T1F were applied as seed treatment and soil drench. Plants height, number of branch, and fruits weight were measured every one and ten weeks after virus inoculation. Virus concentration in plants and disease incidence were confirmed by enzyme-linked immunosorbant assay (ELISA). Results showed that inoculation with PGPR improved the seed germination. Eight days after sowing, the percentage of PGPR treated seed germination reached 50-84%; whereas those of untreated seed reached only 18%. In general, PGPR treatment significantly reduced (p < 0.05) the effect of virus infection on plant growth. Two PGPR isolates, i.e. BTP1 and BTP2H, maintained fruit weight of infected plants as good as those of healthy plants. Based on ELISA, PGPR was able to inhibit the disease incidence. The BTP3O and BTP2D isolates even protected the plant from ChiVMV infection. Concentration of salicylic acid and peroxidase were relatively higher on plants treated with PGPR than those without PGPR treatment. This gave an indication that PGPR may act as induction agents for systemic acquired resistance. Therefore, PGPR treatment is a promising strategy to control viral diseases on chillipepper.
Critical Period of Tomato toAlternariasokuri (Ell. &amp; G. Martin) Sor. infection and its determinant. Determination of critical period (susceptible period) of plant is one of the strategies in Integrated Pest Management (LPM), because it can reduce or minimize pesticide use. Study about the critical penendo of tomato was cam&#039;ed out in the field of Bangbayang village, in the Cicwug district of Sukubumi. Tomato plant cultivar Zntan at dzremnt age (50, 60, 70 and 80 &amp;ys after sowing [DAS Sientje Mandang Sumaraw
Buletin Hama dan Penyakit Tumbuhan Vol. 11 No. 2 (1999): Buletin Hama dan Penyakit Tanaman
Publisher : Buletin Hama dan Penyakit Tumbuhan

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Abstract

Critical Period of Tomato toAlternariasokuri (Ell. &amp; G. Martin) Sor. infection and its determinant. Determination of critical period (susceptible period) of plant is one of the strategies in Integrated Pest Management (LPM), because it can reduce or minimize pesticide use. Study about the critical penendo of tomato was cam&#039;ed out in the field of Bangbayang village, in the Cicwug district of Sukubumi. Tomato plant cultivar Zntan at dzremnt age (50, 60, 70 and 80 &amp;ys after sowing [DAS] were inoculated with 15 ml of Id s p o ~ l suspension of Alternaria solani per plant, that have been added with Agristick 2% and llveen 80 2%. Observation that was conducted include disease severity, the height of plant, dry weight of plant biomass and total Jiuit production. Leaves of diferent age (50, 60, 70, 80 DAS) at d@rent part of plant (lower, middle and upper canopy) were analyzedfor totalprotein by Auto Analyzer ZZMethod, totalphenol by Follin- Denis Method and total sugar content by L u ~ h o o rMl ethod. The result showed that the older plant have the higher disease severity. The same trend can be seenjkm the age of leaves i.e. leaves fLom the lower part ofplants (olakr) am more susceptible than the middle and upper leaves. The critical period of the plant is at the age of 50 - 60 DAS. TotalJiuit production is not determined by disease severity, instead it is more determined by the age of plant infected. l%em is a tendency that the interaction among total protein and phenol content efect the susceptibility of the plant to the pathogen as can be seen the following regression equation: Y = 167 - 3.1 9 X2 - 22.58 X3 (R= 0.76).