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All Journal Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung Jurnal Ilmiah Kedokteran Wijaya Kusuma Majalah Kedokteran Andalas
Dorta Simamora
Laboratorium Genetika Medik, Pusat Penelitian Biologi Molekuler Fakultas Kedokteran Universitas Wijaya Kusuma Surabaya
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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INTERLEUKIN-6 (IL-6) LEVEL AS AN INDIKATOR OF PROGRESIVITY RHEUMATOID ARTHRITIS (RA) DISEASE Tania, Putu Oky Ari; Simamora, Dorta; Parmasari, Wahyuni Dyah; Rahmawati, Febtarini
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 3, No 1 (2014): Edisi Maret 2014
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rheumatoid Arthritis (RA) is autoimmune disease that attacks joints and synovial tissues. Rheumatoid Arthritis is characterized by inflammation that involves production of cytokines such as Interleukin 6 (IL-6). Increasing of interleukin 6 has correlation with activity and progressivity of the disease. This research was designed to know whether IL- 6 could be used as indicator progressivity of RA. The method that used for measuring IL-6 level is ELISA (Enzyme Linked Immunosorbent Assay), using the principle antigen-antibody binding. In early stage there were 9 persons (S01, S02, S03, S04, S07, S08, S09, S10 and S14) with of IL-6 level (mean : 3.640 pg/mL), intermediate stage there were 3 persons (S06, S13 and S15) with IL-6 level (mean : 12.453 pg/mL), late stage : 3 persons (S05, S11 and S12) with IL-6 level (mean : 59.349 pg/mL).
Potensi Jus Buah Naga Merah (Hylocereus polyrhizus) terhadap Perbaikan Jaringan Organ Otak Tikus (Rattus norvegicus) Diabetes Putra, I Gede Gelgel Bayu Surya; Simamora, Dorta
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 8, No 2 (2019): EDISI SEPTEMBER 2019 (available online since Oktober 2019)
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (983.726 KB) | DOI: 10.30742/jikw.v8i2.622

Abstract

Diabetes mellitus is a metabolic disease characterized by hyperglycemia due to disturbances in insulin secretion, insulin action, or both. The purpose of this study was to analyze the effect of the red dragon fruit juice (Hylocereus polyrhizus) to repair brain organ histopathologic female wistar rats (Rattus norvegicus Wistar strain) with diabetes. This research is a true experiment research with randomized control - group pretest - posttest design and were conducted at the in Vivo Laboratory of Wijaya Kusuma University Surabaya. Twenty five female wistar rats were randomly divided into 5 groups, normal rats group KA (without alloxan induction), diabetic rats group KB (alloxan induction and without given any red dragon fruit juices), treatment group KC (alloxan induction +  2 gr/2,5ml of red dragon fruit juices), treatment group KD (alloxan induction + 4 gr/2,5ml of red dragon fruit juices) and treatment group KE (alloxan induction + 8 gr/2,5ml of red dragon fruit juices). The study was conducted during the period of 16 days with standard feeding adaptations for 7 days. On day 16, all the rats were terminated. Glucose meter is used to measure the levels of blood glucose in rats. Histopathologic observation of rat brain tissue using a microscope Olympus CX21 with Haematoksilin eosin (HE) staining. The results showed the best dose of red dragon fruit juices to decrease blood glucose levels compare between KB and KE with significance p-value = 0.000 (? < 0.05), the blood glucose levels decreased in diabetic rats significantly between KB and KE also decreased in rat brain tissue necrosis with p-value = 0.000 (? <0.05).
Kombinasi Ekstrak Batang Talikuning dan Artemisin sebagai Obat Antimalaria terhadap Plasmodium berghei Muti'ah, Roihatul; Enggar F, Loeki; Winarsih, Sri; Soemarko, Soemarko; Simamora, Dorta
Jurnal Kedokteran Brawijaya Vol 26, No 1 (2010)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (645.931 KB) | DOI: 10.21776/ub.jkb.2010.026.01.6

Abstract

ABSTRAKMalaria, penyakit menular dengan tingkat kematian yang tinggi sekarang menghadapi penurunan kemanjuran obat obat pilihan pertama yaitu kombinasi artemisin dan amodiaquine. Talikuning (Anamirta cocculus), adalah jamu tradisional yang secara empiris digunakan sebagai antimalaria di Papua telah disarankan untuk meningkatkan efektivitas kombinasi obat antimalaria. Talikuning batang dan akar mengandung alkaloid kuartener yang dianggap memiliki aktivitas fisiologis sebagai antimalaria. Penelitian ini bertujuan untuk memahami efek antimalaria dari ekstrak batang talikuning dan kombinasinya dengan artemisin pada derajat parasitemia mencit terinfeksi Plasmodium berghei. Peritoneal tikus yang terinfeksi dengan Plasmodium berghei ANKA 106 dan dibagi menjadi 11 kelompok perlakuan, kontrol negatif, kontrol positif; artemisin dosis 0,04 mg / g BB; talikuning dosis: 0,001 mg / g BB; 0,01 mg / g BB; 0, 1 mg / g BB, dan 1 mg / g BB. Dan kombinasi artemisin talikuning dosis: 0,001 mg / g BB; 0,01 mg / g BB, 0,1 mg / g BB; and1 mg / g BB. Pengobatan dimulai pada hari 0 di mana derajat parasitemia mencapai 5-15% dan dilanjutkan selama 7 hari, observasi parasitemia dilakukan pada hari 3, 5 dan 7 hari. Hasil penelitian menunjukkan ekstrak talikuning induk  bisa menghambat pertumbuhan Plasmodium berghei secara signifikan (p <0,05) terhadap kontrol dengan ED50 dari 0,043 mg / g BB tikus yang setara dengan 4,7 mg / kg BB manusia. Namun demikian, kombinasi dosis-talikuning artemisin tidak menunjukkan perbedaan yang signifikan dibandingkan dengan pengobatan monoterapi artemisin (p> 0,05) baik pada hari 3, 5 dan 7 hari pasca terapi. Hal ini menunjukkan bahwa pemberian kombinasi-talikuning artemisin tidak berbeda dari penyediaan artemisin monoterapi.Kata Kunci : Antimalaria,  artemisin, derajat parasitemia, Plasmodium berghei, talikuning
INTERVENSI CYTOADHERENCE SEBAGAI PELUANG UNTUK PENCEGAHAN DAN TERAPI MALARIA BERAT Simamora, Dorta; Sumitro, Sutiman B.; Fitri, Loeki Enggar
Jurnal Kedokteran Brawijaya Vol 22, No 2 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.666 KB) | DOI: 10.21776/ub.jkb.2006.022.02.3

Abstract

Abstract  In  malarial  infection,  erythrocytes  infected  wih  Plasmodium  falciparum  bind  to  endothelial  vascular  (endothelial cytoadherence). This binding is implicated in the forming of sequestre and  rosette that affects the vascular circulation, and thus injures  the capillary wall. This mechanism is important in pathogenesis of malarial due to dysfunction of several organs. There are several receptors of  cytoadherence in human e.g Thrombospondine (TSP), CD-36, ICAM-1, and ELAM-1 as well as specific ligands of the parasite  e.g  Plasmodium falciparum Erythrocyte Membrane Protein-1 (PfEMP-1), 220 kDa protein of Pf60, Pf332, sequestrin, Pfaldhesin and STEVOR.  PfEMP-1 has been revealed as a molecule that is responsible for pathogenesis of severe malaria. This protein can pass parasitophorous vacoular membrane (PVM) of the parasite by attaching its molecule to carry Protein Export Elemen (PEXEL) and than go to the surface of erythrocytes in combination with specific helper protein in maeurer cleft.  The intervention on cytoadherence process through blocking of specific ligand directly or blocking the translocation of this ligand to the surface of erythrocytes might be important in regulating the outcome of malarial infection.  Key words : Cytoadherence, severe malaria, intervention, adhesion molecule.
RESISTENSI OBAT MALARIA: MEKANISME DAN PERAN OBAT KOMBINASI OBAT ANTIMALARIA UNTUK MENCEGAH Simamora, Dorta; Fitri, Loeki Enggar
Jurnal Kedokteran Brawijaya Vol 23, No 2 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1046.929 KB) | DOI: 10.21776/ub.jkb.2007.023.02.4

Abstract

Multi drug resistance (MDR) of malariae parasite iscaused by decreasing ability of some antimalarial  drugs examples chloroquine, mefloquine, antifolate and atovaquone  to Plasmodium. Resistance to artemisin has not beenfound yet. Antimalaria drug resistance happened because some factors especially : genetic factor (gene mutation)  of the Plasmodium. Mechanism pathway of resistance differsin location, because the different target drug action. Resistance migh be reduce by using combination therapy. Key words: resistance, Plasmodium falciparum, antimalaria combination
Efek Kombinasi Ekstrak Anamirta cocculus dan Artemisin terhadap Penurunan Jumlah Sel Apoptosis Jaringan Paru Mencit Malaria Fitri, Loeki Enggar; Syamsuri, Dara Dasawulansari; Simamora, Dorta; Soemarko, Soemarko; Mintaroem, Karyono
Majalah Kedokteran Bandung Vol 45, No 2 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1715.153 KB)

Abstract

Plasmodium dalam eritrosit akan menginduksi respons imun, berupa produksi radikal bebas yang dapat menyebabkan kerusakan jaringan paru. Kombinasi ekstrak Anamirta cocculus dan artemisin diharapkan dapat mencegah komplikasi akibat radikal bebas yang dihasilkan oleh sel imun maupun artemisin. Studi eksperimental dengan metode post test control group design only dilakukan di Laboratorium Parasitologi dan Biomedik Fakultas Kedokteran Universitas Brawijaya periode Juni–Oktober 2010 untuk membuktikan efek pemberian kombinasi ekstrak A. cocculus dan artemisin terhadap jumlah sel apoptosis jaringan paru mencit galur Balb/C yang diinfeksi Plasmodium berghei. Mencit dibagi dalam 6 kelompok, kelompok 1 terdiri atas 9 ekor mencit normal, kelompok 2 mencit yang diinfeksi P. berghei tanpa terapi, kelompok 3 mencit yang diinfeksi P. berghei dan diterapi artemisin dosis 0,04 mg/gBB, serta 3 kelompok perlakuan mencit diinfeksi P. berghei dan diterapi kombinasi artemisin 0,04 mg/gBB dengan ekstrak A. cocculus 0,01 mg/gBB; 0,1 mg/gBB; dan 1 mg/gBB. Sel apoptosis dihitung dari ekspresi caspase-3 pada pewarnaan imunohistokimia. Pemberian ekstrak A. cocculus dosis 0,01 mg/gBB; 0,1 mg/gBB; dan 1 mg/gBB serta artemisin selama 3 hari menurunkan jumlah sel apoptosis secara signifikan dibandingkan dengan kelompok artemisin monoterapi (p=0,00; 0,026; 0,000). Hubungan lama terapi dengan ekspresi caspase-3 menunjukkan pada kelompok terapi kombinasi ekstrak A. cocculus 0,01 mg/gBB dan artemisin 0,04 mg/gBB memiliki korelasi positif yang signifikan (p=0,013). Simpulan, terapi jangka pendek kombinasi ekstrak A. cocculus dan artemisin mempunyai efek yang lebih baik dibandingkan dengan pemberian artemisin monoterapi dalam hal penurunan jumlah sel apoptosis jaringan paru. [MKB. 2013;45(2):69–77]Kata kunci: Anamirta cocculus, apoptosis sel paru, artemisin,caspase-3, malariaThe Effect of Anamirta cocculus Extract and Artemisin Combination in Decreasing Number of Apoptotic Lung Cells of Malaria Infected MiceThe presence of Plasmodium in erythrocytes will induce immune responses, including the production of free radicals which can lead to lung tissue cells damage. Combination therapy of Anamirta cocculus extract and artemisin is expected to prevent complications caused by free radicals produced by immune cells and artemisin. This experimental study which using post test control group design only was done in Laboratory of Parasitology and Biomedic Faculty of Medicine, Brawijaya University on June–October 2010 to prove the effect of combination therapy of A. cocculus extract and artemisin towards the number of apoptotic lung tissue cells of Balb/C mice infected by Plasmodium berghei. Mice were divided into 6 groups, the first normal Balb/C mice, the second mice infected by P. berghei without treatment, the third mice infected by P. berghei and treated with artemisin 0.04 mg/g BW, and 3 combination contains mice infected by P. berghei and treated with a combination of artemisin 0.04 mg/ gBW and A.cocculus extract 0.01 mg/gBW, 0.1 mg/gBW, 1 mg/ gBW respectively. The apoptotic cells of lung tissue were counted from the expression of caspase-3 in immunohistochemical staining. In day 3 combination A. cocculus extract dose 0.01 mg/gBW, 0.1 mg/gBW, 1 mg/ gBW and artemisin reduced the number of apoptotic cells significantly compared to the artemisin monotherapy (p=0.00, 0.026, 0.000). There was a positive corelation between the length of treatment and the expression of caspase-3 (p=0.013) on group that treated with combination of A. cocculus extract 0.01 mg/gBW and artemisin 0,04 mg/gBB. In conclusion, the short treatment of combination A. cocculus extract and artemisin has a better effect than artemisin monotherapy in decreasing number of apoptotic lung tissue cells. [MKB. 2013;45(2):69–77]Key words: Anamirta cocculus, artemisinin, caspase 3, lung apoptotic cell, malaria DOI: http://dx.doi.org/10.15395/mkb.v45n2.91
Analisis dan Prediksi Uji Biokimia Fungsi Liver pada Pemberian Ekstrak Kunyit Putih (Curcuma zedoaria) Setelah dan Sebelum Paparan Parasetamol Dosis Tinggi Puja Ayu Misuari; Satya Yudhayana; Putu Oky Ari Tania; Dorta Simamora
Majalah Kedokteran Andalas Vol 45, No 3 (2022): Online July 2022
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v45.i3.p308-315.2022

Abstract

Kegagalan fungsi liver yang akut banyak disebabkan oleh penyalahgunaan parasetamol atau Acetaminophren. Parasetamol dosis tinggi menyebabkan nekrosis liver dan kegagalan fungsi liver. Pada liver, parasetamol diubah menjadi N-acetyl-para-benzo-quinine imine (NAPQI), yang meningkatkan stress oksidatif dan disfungsi mitokondria. Disfungsi liver ditandai dengan peningkatan enzim SGOT dan SGPT serta rasio keduanya. Penggunaan rimpang Curcuma zedoaria dipercaya dapat memberikan proteksi terhadap liver. Kandungan antioksidan fenolik di dalamnya memiliki kemampuan menangkal radikal hidroksil, sehingga diharapkan dapat memulihkan stres oksidatif, melindungi terhadap paparan parasetamol. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak rimpang Curcuma zedoaria setelah dan sebelum pemberian parasetamol dosis yang tinggi, melalui kadar enzim SGOT dan SGPT, serta rasio keduanya. Penelitian ini menggunakan 24 ekor tikus jantan dewasa (Rattus norvegicus) yang dibagi menjadi 4 kelompok yaitu KN; KP yang diberikan parasetamol dosis tinggi 1,35 g/ Kg BB; kelompok perlakuan yang diberi ekstrak Curcuma zedoaria dosis 105mg/ 200 g BB pada 2 jam sesudah (P1) dan sebelum pemberian parasetamol (P2). Analisis data menggunakan uji Kruskal-Wallis dan ANOVA dengan nilai p sebesar 0,019 dan 0,027. Penurunan kadar SGOT dan SGPT terendah pada kelompok P2 yaitu 156,06 IU dan 151,90 IU. Rasio SGOT/SGPT tertinggi pada KP sebesar 2,21 IU/L.
Interleukin-6 (IL-6) Level as an Indikator of Progresivity Rheumatoid Arthritis (RA) Disease Putu Oky Ari Tania; Dorta Simamora; Wahyuni Dyah Parmasari; Febtarini Rahmawati
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 3, No 1 (2014): Edisi Maret 2014
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.884 KB) | DOI: 10.30742/jikw.v3i1.44

Abstract

Rheumatoid Arthritis (RA) is autoimmune disease that attacks joints and synovial tissues. Rheumatoid Arthritis is characterized by inflammation that involves production of cytokines such as Interleukin 6 (IL-6). Increasing of interleukin 6 has correlation with activity and progressivity of the disease. This research was designed to know whether IL- 6 could be used as indicator progressivity of RA. The method that used for measuring IL-6 level is ELISA (Enzyme Linked Immunosorbent Assay), using the principle antigen-antibody binding. In early stage there were 9 persons (S01, S02, S03, S04, S07, S08, S09, S10 and S14) with of IL-6 level (mean : 3.640 pg/mL), intermediate stage there were 3 persons (S06, S13 and S15) with IL-6 level (mean : 12.453 pg/mL), late stage : 3 persons (S05, S11 and S12) with IL-6 level (mean : 59.349 pg/mL).
Pengaruh Pemberian Minuman Energi Terhadap Kadar Serum Kreatinin Rattus Norvegicus Budi Santo; Dorta Simamora
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 10, No 1 (2021): MARET 2021
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.255 KB) | DOI: 10.30742/jikw.v10i1.1045

Abstract

The energy drink is a beverage that is increasingly being used by the community with the aim to improve work performance, vitality, and concentration. The energy drink is also suspected to affect kidney function. This study aims to determine whether there is influence of various brands of energy drinks (M1, M2, M3, M4) of the serum creatinine levels in male rats Rattus norvegicus.This is a study with randomized post test only control group design. The study used male rats Rattus norvegicus as many as 25 animals that were divided into 5 groups. The control group, the group P1 = M1 energy drinks, group P2 = M2 energy drinks, group P3 = M3 energy drinks, group P4 = M4 energy drinks. The energy drinks were given as many as 2.5 ml / 200 g weight / day for 1 month. Cardiac blood sampling performed to measure the levels of serum creatinine and then the results were analyzed using One-Way Anova to compare the levels of serum creatinine in each group of this study. Results of One-Way Anova test obtained by sig α = 0.000 which means there is significant difference between the treatment group and the control group. It can be concluded that the administration of energy drinks to the Rattus norvegicus would be altered the kidney function. It is caused by various substances contained in energy drinks such as taurine, sugar, vitamin B, caffeine, and other supplementary materials.
Potensi Jus Buah Naga Merah (Hylocereus polyrhizus) terhadap Perbaikan Jaringan Organ Otak Tikus (Rattus norvegicus) Diabetes I Gede Gelgel Bayu Surya Putra; Dorta Simamora
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 8, No 2 (2019): EDISI SEPTEMBER 2019 (available online since Oktober 2019)
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (983.726 KB) | DOI: 10.30742/jikw.v8i2.622

Abstract

Diabetes mellitus is a metabolic disease characterized by hyperglycemia due to disturbances in insulin secretion, insulin action, or both. The purpose of this study was to analyze the effect of the red dragon fruit juice (Hylocereus polyrhizus) to repair brain organ histopathologic female wistar rats (Rattus norvegicus Wistar strain) with diabetes. This research is a true experiment research with randomized control - group pretest - posttest design and were conducted at the in Vivo Laboratory of Wijaya Kusuma University Surabaya. Twenty five female wistar rats were randomly divided into 5 groups, normal rats group KA (without alloxan induction), diabetic rats group KB (alloxan induction and without given any red dragon fruit juices), treatment group KC (alloxan induction +  2 gr/2,5ml of red dragon fruit juices), treatment group KD (alloxan induction + 4 gr/2,5ml of red dragon fruit juices) and treatment group KE (alloxan induction + 8 gr/2,5ml of red dragon fruit juices). The study was conducted during the period of 16 days with standard feeding adaptations for 7 days. On day 16, all the rats were terminated. Glucose meter is used to measure the levels of blood glucose in rats. Histopathologic observation of rat brain tissue using a microscope Olympus CX21 with Haematoksilin eosin (HE) staining. The results showed the best dose of red dragon fruit juices to decrease blood glucose levels compare between KB and KE with significance p-value = 0.000 (α < 0.05), the blood glucose levels decreased in diabetic rats significantly between KB and KE also decreased in rat brain tissue necrosis with p-value = 0.000 (α <0.05).
Co-Authors A.A. Ketut Agung Cahyawan W Budi Santo Dara Dasawulansari Syamsuri Febtarini Rahmawati Febtarini Rahmawati I Gede Gelgel Bayu Surya Putra Karyono Mintaroem Loeki Enggar Fitri Muti'ah, Roihatul Puja Ayu Misuari Putra, I Gede Gelgel Bayu Surya Putu Oky Ari Tania R Abyseka Prayogo Satya Yudhayana Soemarko Soemarko Sutiman B. Sumitro Wahyuni Dyah Parmasari Wahyuni Dyah Parmasari, Wahyuni Dyah