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SNP8NRG433E1006 NEUREGULIN-1 GENETIC VARIATION IN BATAKS ETHNIC WITH SCHIZOPHRENIA PARANOID AND HEALTHY CONTROL Effendy, Elmeida; Loebis, Bahagia; Amir, Nurmiati; Siregar, Yahwardiah
BALI MEDICAL JOURNAL Vol 3 No 2 (2014)
Publisher : BALI MEDICAL JOURNAL

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Abstract

The neuregulin 1 (NRG1) gene which influences the development of white matterconnectivity has been associated with schizophrenia. It influences neuronal migration, synaptogenesis,gliogenesis, neuron-glia communication, myelination, and neurotransmission in the brain and others.NRG1 is located in 8p13, and it is frequently replicated in schizphrenia. SNP8NRG433E1006 geneNRG1 is one of core at risk haplotype of schizphrenia. This study looked forward differencesSNP8NRG433E1006 neuregulin 1 between Bataks ethnic with schizophrenia paranoid and Bataksethnic healthy control. Methods: Batak ethnic with schizophrenia paranoid were recruited andinterviewed with semi-structured MINI ICD-X to establish the diagnosis. All the eligible subjectswere requested their permission for blood sampling. Healthy Batak ethnic were also recruited bymathcing the age and gender. The blood samples went through DNA isolation, Nested PCR, and DNAsequencing. Results: Ninety three subjects were recruited, but only 74 blood samples weresuccesfully sequenced. We found three types of polymorphisms, i.e. G/A allele at base pair (bp) 76,G/T allele at bp 112, and deletion at bp 110 in Batak ethnic with schizophrenia. There were two kindsequences at bp 113-116 in Batak ethnics, and Batak ethnics with ATCG were at higher risk forhaving schizophrenia. This study support that NRG1 is a schizophrenia-susceptibility gene.
Short-term intravenous zoledronic acid in severe osteogenesis imperfecta : A report of three siblings of children Lim, Hadyanto; Zein, Umar; Siregar, Yahwardiah; Marpaung, Blondina; Sinaga, Jaminsen
Medical Journal of Indonesia Vol 17, No 2 (2008): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.322 KB) | DOI: 10.13181/mji.v17i2.310

Abstract

This report documented the clinical and biochemical side effects on the first dose of intravenous zoledronic acid therapy in three siblings with severe osteogenesis imperfecta. Zoledronic acid was administered in 50 ml 0.9% saline solution over a period of 30 minutes. All patients had fever during the first 6 to 48 hours after the first infusion. There were no renal side effects, apart from asymptomatic hypocalcemia and hypophosphatemia at 48 and 72 hours after zoledronic acid infusion. The minimal clinical side effects were easily manageable. (Med J Indones 2008; 17: 127-30)Keywords: zoledronic acid, osteogenesis imperfecta, side effects
ASPERGILLUS FUMIGATUS PADA SPUTUM PENDERITA BATUK KRONIK MENGGUNAKAN METODE PCR DAN KULTUR Thristy, Isra; Siregar, Yahwardiah
Majalah Kedokteran Bandung Vol 48, No 2 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.224 KB) | DOI: 10.15395/mkb.v48n2.760

Abstract

Kecepatan tumbuh jamur yang bertambah pada manusia dapat terjadi akibat berbagai penggunaan obat-obatan, alat-alat kesehatan invasif, juga terdapat faktor predisposisi berupa penyakit kronik yang berat termasuk penyakit keganasan. Aspergilus fumigatus merupakan salah satu spesies jamur sistemik yang dapat menginfeksi manusia. Penggunaan polymerase chain reaction (PCR) untuk mendeteksi jamur merupakan diagnostik yang optimal. Tujuan penelitian ini adalah menganalisis Aspergillus fumigatus menggunakan PCR dan kultur pada sputum penderita batuk kronik. Penelitian ini menggunakan 51 sampel sputum penderita batuk kronik yang berasal dari Rumah Sakit Haji Adam Malik Medan, periode Mei 2012?Februari 2013 diteliti dengan metode deskriptif cross-sectional. Sputum diperiksa menggunakan metode PCR dan dilakukan kultur sebagai gold standard pemeriksaan jamur. Hasil penelitian dari 51 sampel didapatkan pemeriksaan PCR positif 35 sampel (69%) dan PCR negatif 16 sampel (31%). Pada hasil kultur dijumpai kultur positif 29 sampel (57%) dan kultur negatif 22 sampel (43%). Simpulan dari hasil penelitian ini adalah dari 51 sputum penderita batuk kronik ternyata 69% mengandung jamur Aspergillus fumigatus dan keadaan ini diperkuat dengan hasil kulturnya. Pemeriksaan PCR dapat dipergunakan sebagai salah satu pemeriksaan jamur yang cepat dan tepat. [MKB. 2016;48(2):78?83]Kata kunci: Aspergillus fumigatus, batuk kronis, kultur, PCRAspergillus Fumigatus in Sputum of Patients with Chronic Cough Using PCR and Culture MethodAbstractThe increasing prevalence of fungal growth in humans may occur as a result of the use of various drugs, invasive medical devices, and also the presence of predisposing factors like severe chronic diseases, including malignant disease. Aspergillus fumigatus is a systemic fungal species that can infect human beings. Polymerase chain reaction (PCR) is the most optimum diagnostic method to detect fungus. The purpose of this study was to analyze the presence of Aspergillus fumigatus by using PCR and culture method in sputum of patients with chronic cough.  The sample of this study consisted of sputum from 51 patients who visited Haji Adam Malik Hospital in Medan. The examination applied the cross-sectional descriptive method. Sputum was examined using PCR and culture as the gold standard fungal examination. The results from the 51 samples showed that positive PCR results (69%) were found in 35 patients and negative results were found in 16 patients (31%). The culture results showed that positive cultures were seen in 29 samples (57%) and negative cultures were found in 22 samples (49%).  it is concluded that from 51 patients with chronic cough sputum, 69%  turn out to have Aspergillus fumigatus fungus and this result is reinforced by the culture results. PCR can be used as a quick and precise inspection method for detecting fungus. [MKB. 2016;48(2):78?83]Key words: Aspergillus fumigatus, chronic cough, culture, PCR
Association of ATP-binding cassette sub-family B member 1 gene C3435T polymorphism with neutropenia in breast cancer patients treated with chemotherapy Syarifah, Siti; Siregar, Kamal B.; Siregar, Yahwardiah
Medical Journal of Indonesia Vol 25, No 3 (2016): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.982 KB) | DOI: 10.13181/mji.v25i3.1326

Abstract

Background: Neutropenia is the most common adverse event of breast cancer chemotherapy which can be life threatening due to opportunistic infection, neutropenic episodes may lead to delay or reduction of drug doses which may compromise treatment outcomes. In this study, we investigated the association of ATP-binding cassette sub-family B member 1 (ABCB1) gene C3435T polymorphism with the grading of neutropenia in breast cancer patients who treated with doxorubicin-taxan.Methods: 72 Indonesian female breast cancer patients from Haji Adam Malik Hospital who had been diagnosed and treated with doxorubicin-taxane regimen were selected for this cohort study. DNA was extracted from peripheral leucocytes and ABCB1 C3435T polymorphism was analyzed with PCR-RFLP. Patient data were collected from patient’s medical record for 3 cycles of chemotherapy. Association between ABCB1 C3435T polymorphism with neutropenia was assessed using Kruskal-Wallis test. Decline of absolute neutrophil count was assessed using Wilcoxon test. Genotype deviation and allele frequencies were also determined by Hardy-Weinberg Equilibrium.Results: The frequencies of ABCB1 C3435T genotype for wildtype (CC), heterozygous (CT) and homozygous mutant (TT) was 22 (30.6%), 38 (52.8%) and 12 (16.7%) respectively. No association were found between ABCB1 C3435T polymorphism and the grading of neutropenia (p>0.05). There was a difference on the average of absolute neutrophil count after the first chemotherapy and after the third chemotherapy (p<0.05). There was no significant deviation of allele and genotype frequency from Hardy-Weinberg Equilibrium.Conclusion: ABCB1 C3435T polymorphism had no association with the grading of neutropenia in breast cancer patients treated with doxorubicin-taxane regimen, however there was a trend of absolute neutrophil count declining during the 3 cycles of chemotherapy.
miR-21 Expression in Grade I, II, and III Breast Cancer -, Juwita; Ompusunggu, Henny E.S.; Nauphar, Donny; Pardede, Sumondang M.; Lim, Hadyanto; Siregar, Yahwardiah
Indonesian Journal of Medicine Vol 1, No 1 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: Breast cancer is the second largest cancer in the world.The gold standard for breast cancer diagnosis and prognosis is grade assessment (mitotic, nuclear pleiomorphism, tubular formation), based on breast tissue histopathology. However, this assessment has limitation in that it is subjective and semi quantitative. A genetic biomarker known as  microRNA (miRNA) may regulate human gene expression by targetting mRNA. One of the miRNA, i.e. miR-21, is able to suppress tumor suppressor mRNA, resulting in increased cellular proliferation andneoplastic transformation, and decreased apoptosis. This study aimed to investigate the expression of miR-21in breast cancer with histopathologic grade I, II, and III.Subjects and Method: This was a descriptive study with cross-sectional design involving paraffin block (FFPE) samples of ductal-type breast cancer tissue. Sixty four samples were obtained from the department of Anatomical Pathology, Haji Adam Malik Hospital, Medan. Patients history were obtained through medical records followed by sample processing (Total RNA Isolation, cDNA Synthesis, and Examination of miR-21 Expression using Real-Time qPCR). MiR-21 expression from every hystopathologic grade was obtained using Fold Change Calculation.Results: Breast cancer occurred more frequently in age group of 30-49 years old. Based on the histopathologic grade, most breast cancer was found in grade II. Analysis of miR-21 expression showed that miR-21 expression was higher in grade III compared to other grade.Conclusion: Breast cancer occured more frequently in the productive age group.Most breast cancer were found in grade II. Increased miR-21 expression in grade III may suggest that miR-21 is an oncomiR, which plays an important role in the development of malignant breast cancer.Keywords: breast cancer, histopathology grade, miRNA, miR-21.Correspondence: Juwita. Masters Program in Biomedical Sciences, Faculty of Medicine, North SumateraUniversity, Medan. Mobile: 08125262339.Indonesian Journal of Medicine (2016), 1(1): 26-33https://doi.org/10.26911/theijmed.2016.01.01.04
MicroRNA-155 Tissue Expression in Patients with Breast Cancer at Dr Haji Adam Malik Hospital, Medan Ompusunggu, Henny Erina Saurmauli; -, Juwita; Nauphar, Donny; Pardede, Sumondang M.; Lim, Hadyanto; Siregar, Yahwardiah
Indonesian Journal of Medicine Vol 1, No 1 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: MicroRNA (miRNA) is noncoding-RNA that comprises 18-22 nucleotides. miRNA binds its gene targets in the 3-untranslated region (3-UTR), causing direct degradation of mRNA and repressed translation of RNA. MicroRNA regulates various biological processes, including cell proliferation, apoptosis, cell growth, cell differentiation, and metabolism. Various changes have been identified miRNA expression in different types of cancers. This study aimed to describe the expression of miR-155 in patients with breast cancer tissue in Haji Adam Malik Hospital in Medan.Subjects and Method: This was a descriptive study. A total sample was 64 paraffin tissue blocks was taken from female patients with breast cancer at the surgical oncology unit, Haji Adam Malik Hospital in 2013-2014. RNA sample were isolated and checked using a spectrophotometer followed by cDNA synthesis. Then this sample was analyzed for miR-155 expression by using Real Time qPCR. Histopathological grade data were obtained from the medical record.Results: The miR-155 expression in the breast cancer patients is higher than that in the UniSp6 RNA Spike-in (internal controls). miR-155 expression was 29.64, 28.44, 29.22, in grade-I, grade-II, grade-III breast cancer, respectively. UniSp6 RNA Spike-in was 22.30, 22.89, 23.10, respectively in the control groups. Thus, increased of miR-155 expression was the highest in grade I breast cancer patients.Conclusion: Increased miR-155 expression can be used as a biomarker for early diagnosis and prognosis of breast cancer.Keywords: miRNA, miR-155, breast cancer.Correspondence: Henny Erina Saurmauli Ompusunggu. Department of Cellular and Molecular Biology, Faculty of Medicine, HKBP Nommensen University, Medan. Email: ompusunggu.henny@gmail.comIndonesian Journal of Medicine (2016), 1(1): 20-25https://doi.org/10.26911/theijmed.2016.01.01.03
Relationship between Body Mass Index, Age, and Muscular Endurance among Soccer Players in Medan, North Sumatra Sarah, Dewi Maya; Siregar, Yahwardiah; Eyanoer, Putri Chairani
Indonesian Journal of Medicine Vol 4, No 1 (2019)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: Maintaining body mass index is one of the social problems, especially for young athletes. This study aimed to determine the relationship of body mass index (BMI) and age to muscular endurance in soccer athletes in Medan.Subjects and Method: This was a cross-sectional study soccer clubs in Medan, North Sumatera, Indonesia, in April 2018. A sample of 96 soccer players was selected for this study. The dependent variable was muscular endurance. The independent variables were BMI and age. Body weight was measured by weight scale. Body height was measured by microtoise. The other data were collected by questionnaire. Muscle endurance was assessed by 1 minute push up test. The data were analyzed by Person product moment.Results: BMI was positively correlated with muscle endurance but it was not statistically significant (r = 0.09; p = 0.390). Age was positively correlated with muscle endurance and it was statistically significant (r= 0.62; p= 0.001).Conclusion: BMI and age are positively correlated with muscle endurance among soccer players.Keywords: BMI, muscle endurance, soccer athleteCorrespondence: Dewi Maya Sarah. Masters Program in Biomedics, Faculty of Medicine, Universitas Sumatera Utara. Jl. Dokter Mansur, Medan, Sumatera Utara, Indonesia. Email: dewidewi­maya­sarah­@ymail.com. Mobile: 081295583909.Indonesian Journal of Medicine, (2019), 4(1): 21-27https://doi.org/10.26911/theijmed.2019.04.01.04
Hubungan Kadar Vitamin D Plasma dengan Indeks Mitosis pada Pasien Kanker Payudara Pratiwi, Astrid Siska; Siregar, Yahwardiah
Sriwijaya Journal of Medicine Vol. 1 No. 3 (2018): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

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Abstract

Breast cancer is the most common cancer suffered by women and one of the biggest causes of death for women worldwide. Later, nutrition became one of the risk factors in breast cancer outcome, one of them is vitamin D. Calcitriol is the active form of vitamin D as an anti-proliferation, and inhibits tumor growth. Uncontrolled proliferation is one of the characteristics of cancer cells. Tumor growth rates become prognostic markers that can be evaluated by correlating at cellular levels such as mitosis. The aimed of this study was to analyze the relationship between vitamin D levels and mitosis index in breast cancer patients. Research with cross sectional design gathered 50 breast cancer patients who were newly diagnosed and had never received chemotherapy. The study was conducted from January 2017 - August 2018 at the Department of Surgical Oncology, RSUPH. Adam Malik Medan. Examination of plasma vitamin D levels is done by ELISA technique at the Lab. Faculty of Medicine Universitas Sumatera Utara. The mitosis index data was obtained from the medical record of the research subjects reviewed by anatomical pathology specialists at the Lab. Anatomical Pathology of Faculty of Medicine Universitas Sumatera Utara. Statistical test uses Fisher Exact. The results of the study 48% of subjects had vitamin D sufficiency, 38% insufficiency and 14% had deficiency. The mean vitamin D levels in all of the study subjects were insufficiency (27.93ng / ml). In this study there was no significant relationship between plasma vitamin D levels and mitosis index in breast cancer patients p = 0.062. The insignificant relationship between vitamin D levels and mitotic index in this study shows that vitamin D not a single factors to influence the mitosis index in breast cancer patients.
Hubungan Kadar Vitamin D Plasma dengan Indeks Mitosis pada Pasien Kanker Payudara Pratiwi, Astrid Siska; Siregar, Yahwardiah
Sriwijaya Journal of Medicine Vol. 1 No. 3 (2018): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

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Abstract

Breast cancer is the most common cancer suffered by women and one of the biggest causes of death for women worldwide. Later, nutrition became one of the risk factors in breast cancer outcome, one of them is vitamin D. Calcitriol is the active form of vitamin D as an anti-proliferation, and inhibits tumor growth. Uncontrolled proliferation is one of the characteristics of cancer cells. Tumor growth rates become prognostic markers that can be evaluated by correlating at cellular levels such as mitosis. The aimed of this study was to analyze the relationship between vitamin D levels and mitosis index in breast cancer patients. Research with cross sectional design gathered 50 breast cancer patients who were newly diagnosed and had never received chemotherapy. The study was conducted from January 2017 - August 2018 at the Department of Surgical Oncology, RSUPH. Adam Malik Medan. Examination of plasma vitamin D levels is done by ELISA technique at the Lab. Faculty of Medicine Universitas Sumatera Utara. The mitosis index data was obtained from the medical record of the research subjects reviewed by anatomical pathology specialists at the Lab. Anatomical Pathology of Faculty of Medicine Universitas Sumatera Utara. Statistical test uses Fisher Exact. The results of the study 48% of subjects had vitamin D sufficiency, 38% insufficiency and 14% had deficiency. The mean vitamin D levels in all of the study subjects were insufficiency (27.93ng / ml). In this study there was no significant relationship between plasma vitamin D levels and mitosis index in breast cancer patients p = 0.062. The insignificant relationship between vitamin D levels and mitotic index in this study shows that vitamin D not a single factors to influence the mitosis index in breast cancer patients.
Correlation of ACE Gene Polymorphism and Hypertension in Stroke Ischemic Patients Chandra, Vincent Alexander; Siregar, Yahwardiah; Arina, Cut Aria
Sumatera Medical Journal Vol. 3 No. 1 (2020): Sumatera Medical Journal (SUMEJ)
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/sumej.v3i1.3310

Abstract

Stroke is the third highest cerebrovascular disease in the world with high mortality and disability rate that is mostly dominated by ischemic stroke. Genetic factor that had been reported to have an indirect effect in increasing the incidence of ischemic stroke is ACE gene polymorphism. ACE gene polymorphism is characterized by the insertion marked by letter (I) or deletion marked by letter (D) on intron 16, chromosome 17. ACE gene polymorphism has drawn a lot of attention from scientists and had been reported to have an indirect effect in increasing the ischemic stroke incidence through pathogenesis of hypertension and atherosclerosis. In this study, 78 subjects of ischemic stroke consist of 43 subjects with hypertension and 35 subjects with normotension. I allele of ACE gene polymorphism was more dominant than D allele in hypertensive ischemic stroke patients (72.1% > 27,9%) and this dominance was also seen in the incidence of hypertension vs normotension (55.4% > 44.6%). However, the correlation of ACE gene polymorphism with the incidence of hypertension was not statistically significant when compared based on its genotype (p=0.280) and allele (p=0.948).