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All Journal Jurnal Teknologi Dan Industri Pangan Zuriat Jurnal AGROTEKNOLOGI Jurnal Ilmu Dasar e-Journal Pustaka Kesehatan Berkala Ilmiah Pertanian BERKALA SAINSTEK Agrovigor: Jurnal Agroekoteknologi Indonesian Journal of Agricultural Science UNEJ e-Proceeding Jurnal Bioteknologi & Biosains Indonesia (JBBI) Journal of Livestock Science and Production Indonesian Journal of Chemistry Jurnal Bioindustri (Journal of Bioindustry)
Tri Agus Siswoyo
Program Studi Magister Bioteknologi, Universitas Jember, Jl. Kalimantan, Kampus Tegal Boto, Jember 68121, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Mathematics Physics Veterinary

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Effectiveness of Added L-Ascorbyl Palmitate Antioxidant Synthezed Enzymatically in Coconut Oil Tri Agus Siswoyo; Martiyas Pujirahayu
Jurnal ILMU DASAR Vol 10 No 1 (2009)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.621 KB)

Abstract

L-ascorbyl palmitate (AsA-Pal-Enz) was synthesized by using the immobilized lipase from Aspergillus niger. The antioxidative activity of AsA-Pal-Enz was investigated in coconut oil at different temperatures within a range of 30(RT)-120oC. The effectiveness of AsA-Pal-Enz in coconut oil was monitored by the rate of formation of hydroperoxides, conjugated dienes (K232) and decomposition of hydroperoxides (K270). Samples in storage experiments were periodically removed and analyzed for peroxide value, K232, K270 and radical scavenging activity (DPPHr). Application of AsA-Pal-Enz have markedly reduced the rate of peroxidation in coconut oil during incubation time at RT, 60 and 80oC but at 120oC the rate of peroxidation slightly increased. It has shown that the effectiveness of AsA-Pal-Enz antioxidant in coconut oil was strongly depend on temperature and the time of incubation.
Isolation and Characterization of the Expression of Gene for Sucrose Transporter Proteins in Sugarcane Plant (Saccharum officinarum) Harianti Novita; Tri Agus Siswoyo; Bambang Sugiharto
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.143 KB)

Abstract

Sucrose as the major transported form of fixed carbon must be translocated from source tissue to the sites of consumption and storage or sink tissues. The translocation of sucrose is facilitated by some distinct sucrose transporters proteins (SUT). To study sucrose transporters in sugarcane, we had conducted isolation and characterization of gene encoding sucrose transporter protein. The isolation was performed with RT-PCR method using total RNA isolated from sugarcane leaf and primer designed from conservative region of SUTcDNAs of SoSUT2A (accession number AY65599), OsSUT-1(accession number AAP54842, OsSUT-1 mRNA (accession number XM 46477). Based on the conservative amino acids sequences of QILQQFA and MGKTEPV, the corresponding sequences of the primers for RT-PCR were: P1, (forward) 5’CAGATCCTTCAACAGTTCGC-3’ and P2 (reverse) 5’-TGCCCTTTGTCTCCGGAACC-3’, respectively. Agarose gel electrophoresis shown a clear single 0.5 kb cDNA band of the PCR product. Thus, the DNA was cloned into pGEMT vector (Promega) for further analysis. Sequence determination of the PCR product revealed a nucleotides sequence of 543 bp in length and has a high homology around 89%, 87.3 % and 84.8 % with maize ZmSUT-1, sugarcane SoSUT 2A and rice OsSUT-1 mRNA, respectively. We designated the cDNA as SoSUT2 and the nucleotide sequence have been submitted to GenBank data base under accession number bankit 734628. By using PSORT analysis the fragment of cDNA-SoSUT2 encoded protein may be located in the endoplasmic reticulum. To have a better understanding, the expression of SoSUT2 gene in sugarcane was determinate by RT-PCR method using total RNA isolated from leaf, petioles, stem and root and visualized the PCR product in agarose gel electrophoresis. Based on the cDNA bands intensity, it can be illustrated that the expression of SoSUT2 gene were found highest in sugarcane leafs then petioles and stem, but the expression was not found in root. Although the SoSUT2-cDNA has not been isolated in full size, the results suggest the presence of gene family of SUT in sugarcane.
Antioxidant Activity Study And Α-glucosidase Inhibitor Phenolic Leaf Extract Bungur (Lagerstroemia speciosa) From Meru National Park Betiri Jainur Rochman; Tri Agus Siswoyo; A.A. Istri Ratnadewi
Jurnal ILMU DASAR Vol 17 No 1 (2016)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.651 KB) | DOI: 10.19184/jid.v17i1.2672

Abstract

Study activities phenolic extract of bungur leaf as an antioxidant and inhibitor for the enzyme α-glucosidase was performed by spectrophotometric method. The extraction was carried out by three solvents with different polarity level in order to get three types of extracts namely hexane extract Bungur (HB), ethyl acetate extract Bungur (EAB), and the methanol extract Bungur (MB). The units, used in the analysis, is the total phenolic that standardized in gallic acid. The antioxidant activity of phenolic extracts Bungur leaf can be seen from its ability to reduce free radicals through the damping test DPPH radical, superoxide anion, and hydroxyl. Potential extract phenolic leaf Bungur as an inhibitor of α-glucosidase were analyzed by inhibition of α-glucosidase, but in this study also tested the inhibition of the enzyme α-amylase because both of these enzymes worked on the digestive system and hydrolyze carbohydrates. The results generally showed that the methanol extract of leaves Bungur potential as antioxidants that compared with standard vitamin C. In addition, it had as well as potential for inhibitors of α-glucosidase and α-amylase that compared akarbosa standard, which is expected to be a natural antidiabetic agent.Keywords: phenolic, bungur, antioxidant, inhibitor, α-amilase, α-glukosidase 
TEKNOLOGI PEMANFAATAN LIMBAH PADAT PENGOLAHAN KERIPIK SINGKONG MENJADI PAKAN PELET AYAM PEDAGING DI DESA BARATAN KABUPATEN JEMBER Listya Purnamasari; Nurul Pratiwi; Tri Agus Siswoyo
Journal of Livestock Science and Production Vol 2, No 1 (2018): Journal of Livestock Science and Production
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jalspro.v2i1.687

Abstract

      Baratan Village, Patrang Sub-district, Jember District has a high potential production of cassava chips by Home Industry (IRT). Solid waste of cassava (cassava husk) from cassava processing into cassava chips has not been utilized properly. Cassava husk can be used as raw material for animal feed. The purpose of this program is the empowerment of the community of Baratan IRT in the processing of cassava husk into broiler feed and to increase the value of the waste and can improve the economic of the IRT in Baratan Village. Cassava husk has a deficiency because of the antinutrient content (Cyanide Acid) and dangerous when consumed. The methods used in this program are counseling and chipping of cassava with chipping tools, counseling and training of utilitation of cassava waste, counseling and training of broiler feed formulation, pelet making, mentoring, monitoring and evaluation. The result of this program is the IRT Baratan Village Jember Regency is able to process the solid waste from processing of cassava chips into broiler feed (pelet). Guidebooks can be used by IRT well so they can treat the waste into feed independently. IRT cassava chips as a partner of this program can implement and apply the technology that has been given and strive to always continuous in the manufacture of feed and pellet product development in an effort to improve the welfare and sustainability of the program.Keywords: cassava waste, pelet feed, Baratan Village
Thermodynamic and Thermomicroscopy Study of Atorvastatin Calcium-Succinic Acid Binary Mixtures Yudi Wicaksono; Budipratiwi Wisudyaningsih; Frida Oktaningtias Widiarthi; Tri Agus Siswoyo
Indonesian Journal of Chemistry Vol 17, No 3 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.374 KB) | DOI: 10.22146/ijc.25089

Abstract

Binary mixtures of pharmaceuticals significantly affect the physical and chemical properties of each component. The aim of this work was to explore the thermal behavior and solid state transformation of binary mixture of atorvastatin calcium and succinic acid. The thermodynamics of binary mixtures of atorvastatin calcium - succinic acid were determined by differential scanning calorimeter. Meanwhile, thermomicroscopy and microstructure were determined by a polarized microscope equipped with a heating stage and camera. The results showed that melting points of atorvastatin calcium and succinic acid respectively were 159.35 and 188.51 °C. The solid-liquid phase diagram of atorvastatin calcium - succinic acid indicates the existence of two eutectic points at 136.57 °C and 120.96 °C respectively on the mole fraction of atorvastatin calcium 0.3 and 0.5. Tamman diagram accurately shows mole fraction of atorvastatin calcium at eutectic point 0.33 and 0.46 respectively for eutectic points 130.0 °C and 134.0 °C. Determination of Jackson’s roughness parameter showed a value of atorvastatin calcium, succinic acid and eutectic mixtures > 2 which indicates that the interfaces of remelting crystals were smooth. Microstructure of remelting crystal of atorvastatin calcium and succinic acid respectively was irregular form and crossed plates. The results of thermomicroscopy of binary mixtures of atorvastatin calcium-succinic acid were consistent with differential scanning calorimetry curves and solid-liquid phase diagram.
Preparation and Characterization of a Novel Cocrystal of Atorvastatin Calcium with Succinic Acid Coformer Yudi Wicaksono; Dwi Setyawan; Siswandono Siswandono; Tri Agus Siswoyo
Indonesian Journal of Chemistry Vol 19, No 3 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (21.219 KB) | DOI: 10.22146/ijc.35801

Abstract

Preparation and characterization of a novel cocrystal of atorvastatin calcium with succinic acid coformer were successfully performed. This research aims to modify the crystalline form of atorvastatin calcium through cocrystallization with succinic acid coformer. The cocrystal was prepared by a solvent evaporation method and characterized by Powder X-Ray Diffraction (PXRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The atorvastatin calcium-succinic acid cocrystal has new crystalline peaks at 2θ of 12.9, 18.2 and 26.7° indicating the formation of a new crystalline phase. The cocrystal showed the melting point at 205.7 °C with an enthalpy of fusion 30.2 J/g which is different from the initial components. The FTIR spectra of cocrystal showed the shifting of absorption peaks of groups of initial components indicating of formation of atorvastatin calcium-succinic acid cocrystal through acid–amide intermolecular hydrogen bond interactions. The solubility and dissolution test showed that the cocrystal has solubility and dissolution rate significantly higher than the solubility and dissolution rate of pure atorvastatin calcium.
PENGARUH CEKAMAN KEKERINGAN TERHADAP EKSPRESI GEN KETAHANAN OSCATA DAN OSAPX1 PADA PADI TOLERAN KEKERINGAN Fariza Oktaviani; Irma Novita Sari; Tri Handoyo; Tri Agus Siswoyo; Mohammad Ubaidillah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.196 KB) | DOI: 10.29122/jbbi.v8i2.4857

Abstract

Rice is widely cultivated in Indonesia, where one of the problems is drought. Rice plant growth can be inhibited due to a lack of water, which could cause oxidative stress. One mechanism for self-defence involves activating antioxidative genes. This study aims to determine the regulatory response of OsCATA and OsAPX1 resistance genes in rice under drought stress. The rice varieties used were those already pre-tested, including Siak Raya, Sertani 1, Indragiri, IR64. Drought treatments were 0% (control) and 15% PEG 6000. Results indicated that the interaction of rice variety treatment and drought stress had a highly significant effect on the root length and chlorophyll content but no significant effect on the plant height. Also, the gene expression of OsCATA and OsAPX1 increased in rice plants exposed to drought stress. Variety Sertani 1 was recommended due to their high average increase in root length, reduced average plant height and chlorophyll content, as well as increased gene expression in conditions of drought stress. Padi banyak dibudidayakan di Indonesia, di mana salah satu permasalahannya adalah kekeringan. Pertumbuhan tanaman padi dapat terhambat akibat kekurangan air, yang dapat menyebabkan terjadinya stres oksidatif. Salah satu mekanisme pertahanan diri adalah mengaktifkan gen antioksidatif. Penelitian ini bertujuan mengetahui respons regulasi gen ketahanan OsCATA dan OsAPX1 pada padi yang mengalami cekaman kekeringan. Benih varietas padi yang digunakan sudah diuji pendahuluan sebelumnya, antara lain Siak Raya, Sertani 1, Indragiri, IR64. Perlakuan kekeringan yaitu 0% (kontrol) dan 15% PEG 6000. Hasil penelitian menunjukkan interaksi perlakuan varietas padi dengan cekaman kekeringan signifikan terhadap panjang akar, dan total klorofil namun tidak signifikan terhadap tinggi tanaman. Ekspresi gen OsCATA dan OsAPX1 meningkat pada tanaman padi yang mengalami cekaman kekeringan. Tanaman yang direkomendasikan adalah varietas Sertani 1 yang memiliki peningkatan rata-rata panjang akar yang tinggi, penurunan rata-rata tinggi tanaman, dan kandungan klorofil rendah, serta meningkatnya ekspresi gen pada kondisi cekaman kekeringan.
PENGARUH KONSENTRASI DAN WAKTU APLIKASI CaCl2 TERHADAP FISIKOKIMIA BUAH TOMAT (Lycopersicum esculentum Mill.) Lina Asri Wulandari; Tri Agus Siswoyo; Kacung Hariyono
JURNAL BIOINDUSTRI (JOURNAL OF BIOINDUSTRY) Jurnal Bioindustri: VOL. 2 NO. 1 (2019)
Publisher : Universitas Trilogi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31326/jbio.v2i1.481

Abstract

Tomato was classified as a climacteric fruit with a fast ripening period andthen followed by the increase of fruit softening. The inhibition of fruit softening was needed to maintain the quality and shelf life of the fruit. One of the way to inhibit the tomatoes softening was calcium application. However, the best concentration and timing of calcium application for tomato was still limited. The aimed of research was to determine the best combination of concentration and time of application of calcium chloride (CaCl2) to the physicochemical of tomato. Experiment was arranged in factorial completely randomized design (CRD) with two factors and three replications. First factor was concentration of CaCl2 which consisted of 5 levels (0 M; 0.3 M; 0.6 M; 0.9 M; 1.2 M), while the second factor was application time of CaCl2 (pre- and post-harvesting). The results showed that there was no interaction between concentration and application time of CaCl2 to the physicochemical of tomatoes. Treatment of CaCl2 1.2 M could increased fruit calcium content, inhibited weight loss and fruit softness, increased total titrated acid content, and inhibited the degradation of vitamin C. The application time of CaCl2 in pre- and post-harvest was not significantly different in term of physicochemical properties. Keywords: fruit softness, quality, weight loss, shelf life, vitamin C
PERUBAHAN SENYAWA DAN AKTIVITAS ANTIOKSIDAN PADA RIMPANG JAHE (Zingiber officinale) SELAMA FASE TUMBUH TUNAS Arbi Bahtiar Boedi Iman Halanobis; Tri Agus Siswoyo
Berkala Ilmiah Pertanian Vol 5 No 1 (2022): Februari
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (298.697 KB) | DOI: 10.19184/bip.v5i1.33153

Abstract

Jahe (Zingiber officinale) merupakan tanaman rempah dan herbal yang dimanfaatkan sebagai minuman atau campuran pada bahan pangan. Jahe memiliki nilai jual yang tinggi karena banyaknya permintaan konsumen baik untuk memenuhi kebutuhan rumah tangga maupun sebagai bahan baku perusahaan jamu dan makanan. Permasalahan utama pada budidaya jahe adalah sulitnya menjaga ketersediaan rimpang benih bermutu dalam jumlah cukup pada waktu yang diperlukan. Permasalah tersebut disebabkan oleh rendahnya mutu bahan tanaman, seperti umur panen dan penyimpanan yang tidak tepat. Jahe mengandung senyawa antioksidan alami yang secara farmakologis cukup tinggi dan mampu menghambat radikal bebas. Senyawa antioksidan yang terdapat pada jahe yaitu senyawa fenolik yang berupa golongan flavonoid, turunan asam sinamat, kumarin, dan asam-asam organik polifungsional. Senyawa fenolik pada jahe yang berperan sebagai antioksidan terdiri dari gingerol dan shogaol. Berdasarkan penelitian sebelumnya menyatakan bahwa penyimpanan pada rimpang jahe berpengaruh dalam kandungan senyawa antioksidan pada rimpang jahe. Begitu pula dengan seiring bertambahnya periode pertumbuhan tunas rimpang jahe maka aktivitas antioksidannya semakin meningkat. Penelitian ini bertujan untuk mengetahui mengenai pengaruh umur rimpang pada umur tumbuh tunas terhadap perubahan senyawa dan aktivitas antioksidan. Data yang diperoleh yaitu pada umur rimpang jahe berpengaruh terhadap perubahan senyawa dan aktivitas antioksidan pada rimpang jahe. Umur tunas jahe yang telah melalui proses penyimpanan tidak berpengaruh terhadap perubahan senyawa dan aktivitas antioksidan pada rimpang jahe.
Pengaruh Cekaman Salinitas Terhadap Aktivitas Katalase dan Pertumbuhan Bibit Melinjo (Gnetum gnemon L.) Reza Septyan Pratama; Tri Agus Siswoyo
Berkala Ilmiah Pertanian Vol 5 No 3 (2022): Agustus
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (812.734 KB) | DOI: 10.19184/bip.v5i3.16080

Abstract

ABSTRACTMelinjo plant (Gnetum gnemon L.) is one of the plants that has the potential to be developed, especially inthe tropics. Melinjo plants have a protein content of 9-10%. Reactive Oxygen Species (ROS) have increasedaccumulation in plant cells offset by increased antioxidants, which serve to protect against cell damage causedby osmotic pressure in plant tissue. Salinity stress can be induced through the application of Sodium Chloride(NaCl). This research was conducted to determine the response of growth and activity of the enzyme catalase(CAT) of melinjo seeds to the duration of NaCl application. The study was conducted from January tocompletion in the Green House and plant analysis laboratory at the Faculty of Agriculture, University of Jember.The experimental method used was the Completely Randomized Design (CRD) of NaCl application treatment of0, 5, 10, 15, and 20%. with a treatment duration of application of 45 days and repeated as many as 5 replications.NaCl application of 0 - 20% significantly affects plant growth, especially on plant height, root length, and wetweight parameters. When the condition of the plant experiences salinity stress, it affects the percentage of acidreduction 2,2'- Azinobis (3-ethylbenzatiazolin) - 6-sulfonate (ABTS) has the highest value in the P3 treatment of26.08%. The highest CAT antioxidant activity in P4 treatment was 1.02 units / mg protein.Keywords: NaCl, protein, antioxsidant, Gnetum gnemon L.ABSTRAKTanaman melinjo (Gnetum gnemon L.) merupakan salah satu tanaman yang berpotensi dikembangkankhususnya di daerah tropis. Tanaman melinjo mempunyai kandungan protein sebesar 9-10%. Reactive OxygenSpesies (ROS) mengalami peningkatan akumulasi didalam sel tanaman diimbangi dengan meningkatnyaantioksidan, yang berfungsi melindungi dari kerusakan sel akibat dari tekanan osmotik didalam jaringantanaman. Cekaman salinitas dapat diinduksikan melalui aplikasi Natrium Chlorida (NaCl). Penelitian inidilakukan untuk mengetahui respon pertumbuhan dan aktivitas enzim katalase (CAT) bibit melinjo terhadaplama aplikasi NaCl. Penelitian dilaksanakan pada bulan Januari sampai selesai di Green House dan laboratoriumanalisis tanaman Fakultas Pertanian Universitas Jember. Metode percobaan yang digunakan adalah RALperlakuan aplikasi NaCl 0%, 5%, 10%, 15%, dan 20% dengan perlakuan lama aplikasi 45 hari dan diulangsebanyak 5 ulangan. Lama aplikasi NaCl 0% - 20% berpengaruh nyata terhadap pertumbuhan tanamankhususnya pada parameter tinggi tanaman, panjang akar, berat basah tanaman. Pada saat kondisi tanamanmengalami stress salinitas mempengaruhi persentase peredaman Asam 2,2’- Azinobis (3-etilbenzatiazolin)- 6-sulfonat (ABTS) memiliki nilai tertinggi pada perlakuan P3 sebesar 26,08%. Aktivitas antioksidan CAT tertinggipada perlakuan P4 sebesar 1,02 unit/mg protein.Kata Kunci: NaCl, protein, antioksidan, melinjo
Co-Authors A.A. Istri Ratnadewi Adrian Syawaluddin Siregar Adrian Syawaluddin Siregar Ali Santosa Anandini Aulia Safiera Aprila Iga Mufidah Arbi Bahtiar Boedi Iman Halanobis Ardine Kumalasari Bambang Kusmanadhi Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sukowardojo Bambang Sukowardojo Budipratiwi Wisudyaningsih Budipratiwi Wisudyaningsih Djenal . Djenal Djenal Dwi Setyawan Endah Puspitasari Erfan Efendi Fariza Oktaviani Fitra Dea Wafa Frida Oktaningtias Widiarthi Harianti Novita Ika Oktavianawati Irma Novita Sari Jainur Rochman Kacung Hariyono Kartika Tari Azhar Kumalasari, Ardine Lina Asri Wulandari listya purnamasari Martiyas Pujirahayu Mohammad Ubaidillah Nabila Nur Aisyah Al Ayyubi Norry Eka Palupi Nur Fauziah Matra Nurul Pratiwi Rahmawati Rahmawati Restanto, Didik Pudji Reza Septyan Pratama Sholeh Avivi Sigit Soeparjono Siswandono, Siswandono Sri Hartatik Tri Ardiyati TRI HANDOYO Untung Murdiyanto Untung Murdiyanto Untung Murdiyanto Wuryanti Handayani Yagus Wijayanto Yeni Tri Puspitaningrum Yudi Wicaksono