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Biomonitoring Escherichia coli and Coliform Contamination in Abalone (Haliotis squamata) Cultivation Pond in Musi Village, Gerokgak Sub-District, Buleleng-Bali. Soegianto, Agoes; Wiradana, Putu Angga; Yusup, Deny Suhernawan
Aquacultura Indonesiana Vol 20, No 1 (2019)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.013 KB) | DOI: 10.21534/ai.v20i1.143

Abstract

Abalone (Haliotis squamata) is one of the fisheries commodities that have high economic value. This study was conducted to investigate contamination of Escherichia coli and coliform in Abalone cultivation ponds in the Musi Village area, Buleleng Regency, Bali. An experiment was carried out by taking water samples in the abalone pond every week for one month. The results showed that there was no difference between the total number of Escherichia coli and coliform. Water samples that were positive for coliform in the determination test had no differences in each sample with total coliform values (Colonies / 100mL) of 4, 3, 7 and 9 (MPN / 100mL) respectively. While the total number of Escherichia coli is shown after being grown in EMBA selective media which are 4, 3, 7, 4 (MPN / 100mL) respectively. Meanwhile, when compared with the number referenced from the Decree of the State Minister of Environment Number 51 of 2004 concerning Sea Water Quality Standards, the quality of seawater for aquatic cultivation in this study is still classified as Safe (<1000 MPN / 100mL).
Daya Dukung dan Laju Pertumbuhan Microcystis Hasil Isolasi dari Waduk Sutami pada Berbagai Variasi Konsentrasi Nitrat dan Fosfat dalam Medium Selektif B-12 Retnaningdyah, Catur; Suharjono, Suharjono; Soegianto, Agoes; Irawan, Bambang
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.522 KB) | DOI: 10.24002/biota.v15i3.2590

Abstract

The main objective of this research was to calculate the carrying capacity and growth rate ofisolated Microcystis result in Sutami reservoir on a variety of nitrate and phosphateconcentrations in the B-12 selective medium. Research was conducted in the laboratory withpure experiments using completely randomized factorial design with factors of nitrateconcentration variation (8, 16, 32, and 64 ppm) and phosphate (0.2, 0.4, 0.8 and 1.6 ppm) in B-12medium. Repetition of the study was conducted three times at the same time. Microcystispopulation abundance which was counted every day until the stationary phase (day +30) wasused to calculate the rate of growth (β) and maximum abundance of Microcystis can besupported by each medium treatment (γ). The results showed that the growth rate of Microcystiswas not significantly influenced by levels of phosphate in the medium but significantly positivelycorrelated with increasing nitrate concentration in the medium. Carrying capacity or themaximum abundance (γ) of Microcystis was influenced by the combination of nitrate andphosphate in the B12 medium. Concentration of phosphate 0.4 ppm in medium combined withnitrate 8−64 ppm could support the highest abundance of Microcystis.
Blooming Stimulation of Microcystis in Sutami Reservoir Using Nutrients Nitrate and Phosphate in Different ratio Retnaningdyah, Catur; Suharjono, Suharjono; Soegianto, Agoes; Irawan, Bambang
Journal of Tropical Life Science Vol 1, No 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Microalgae Cyanobacteria from the genus Microcystis has the potential to bloom in Sutami reservoirs that located in Malang, Indonesia at a particular time. Microcystis is a notorious species because it can produce toxins that are dangerous to other organisms. The objective of this research is to determine the influence of different ratios of Nitrate and Phosphate on the growth of Microcystis in Sutami reservoir. The study was conducted from April to November 2009 and was carried out in situ in the reservoir Sutami with quasi experiment using a completely randomized design. Sutami reservoir water which already contained plankton community therein, including Microcystis were treated with five variations ratio of Nitrate and Phosphate (10, 20, 40, 80, and 160) and untreated water as control. Experiments carried out by using a transparent plastic bag diameter 1 m and 1.5 m long, inserted into the water and be immersed in the water by using bamboo. Calculation of Microcystis abundance and physic-chemical quality of water is done every three days for a month. The results showed that the higher concentration of orthophosphate in the media water of Sutami reservoir have the maximum abundance of Microcystis. Abundance of Microcystis grown in Sutami reservoirs have positively correlated with actual levels of nitrate, nitrite, total phosphate, conductivity, pH, temperature, ratio of Nitrate to Phosphate treatment and water KMnO4 value.Keywords: Blooming Microcystis, the ratio of nutrients Nitrate to Phosphate, Sutami reservoir
PENGARUH EDTA DALAM MENGHILANGKAN KADAR KADMIUM YANG TERAKUMULASI DALAM BERBAGAI ORGAN UDANG Macrobrachium sintangense de Man Agoes Soegianto; M. Yusuf Alamudi; Miftakhul Ulum; Eko W. Putro; Nining D. Widyayanti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/412

Abstract

The objective of experiment is to know capability of EDTA to reduce the concentration of cadmium, which accumulated in Macrobrachium sintangense organ. Two groups of shrimps were exposed to 30 mg/L and to freshwater (control) during 7 days and continue with EDTA 0.1 and 1 ppm during 7 days. After finish the experiment, gill, hepatopancreas, carapace and abdominal muscle dissected and destructed with concentrated HNO3 to be analyzed the concentration of cadmium which is accumulated in those organs. The result showed that the concentration of cadmium in gill of control shrimp are 1.696 ± 0.3920 mg/Kg, hepatopancreas 1.1810 ± 0.545 mg/Kg, carapac 0.1025 ± 0.1524 mg/Kg, and abdomen 0.201 ± 0.349 mg/Kg. The concentration of cadmium in exposed shrimps which treated with 0.1 ppm of EDTA are 66.4090 ± 3.22 mg/Kg in gill, 26.4791 ± 0.86 mg/Kg in hepatopancreas, 8.8039 ± 0.66 mg/Kg in carapac; and 3.6324 ± 0.13 mg/Kg in abdomen. The concentration of cadmium in exposed shrimps which treated with 1 ppm of EDTA are 34.8809 ± 4.24 mg/Kg in gills, 9.7290 ± 1.03 mg/Kg in hepatopancreas; 4.6574 ± 0.34 mg/Kg in carapac; and 1.8546 ± 0.43 mg/Kg in abdomen. The greater elimination of cadmium occurs at the shrimp, which is treated with 1 ppm of EDTA.
Pattern of Biogas Production and Removal of Chemical Oxygen Demand in Semi Continues Hybrid Anaerobic Reactor Oktavitri, Nur Indradewi; Soegianto, Agoes; Rahman, T.; Saadah, Bidayatus; Trianingtyas, Deavy
IPTEK Journal of Proceedings Series Vol 1, No 1 (2014): International Seminar on Applied Technology, Science, and Arts (APTECS) 2013
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j23546026.y2014i1.229

Abstract

Anaerobic process had been used for treated highly organic substances, such as canteen wastewater  which had COD around 5000 mg/L. This research aims to investigate pattern of biogas production and COD removal in semi continue anaerobic process. HRT (Hydraulic Retention Times) were used in this research 1,5 hours. The reactor was contained of four cell coulombs from PVC, with height 1 meter. Gravel as media support in hybrid anaerobic reactor. The height of media support is 50 cm. This reactor operated for 14 days. Parameters had been observed are biogas production and COD.. Biogas production after reactor operated 7 days 0,6594 mL and after reactor operated 14 days is 1,4758 mL. COD removals are 38,89 mg/L after operated 7 days and 42,86 mg/L after operated 14 days. In conclusion, COD removal increased after reactor operated 7 and 14 days. The biogas production increased after 7 and 14 operated days. Increasing of biogas production more drastically than COD removal
Utilization of Fertilizers for Improving The Process Rate of Organic Material Degradation in Anaerobic Reactor Adelia Anju Asmara; Agoes Soegianto; Trisnadi Widyaleksono Catur Putranto; Hairul Amin; Nur Indradewi Oktavitri
IPTEK Journal of Proceedings Series Vol 1, No 1 (2014): International Seminar on Applied Technology, Science, and Arts (APTECS) 2013
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j23546026.y2014i1.225

Abstract

This study aimed to assess utilization of fertilizers as nutrient in the anaerobic reactor. Nutrient is one important characteristic of waste for the treatment. Nutrient is required for the growth and reproduction process of microorganisms which involved in the anaerobic process. The wastewater used in this research is tofu wastewater. Gandasil B fertilizer has N 6 mg/l and P 20 mg/l. While for Gandasil D fertilizer has N 20 mg/l and P 15mg/l. Anaerobic reactor has 1000 ml of capacity which contain 600 ml of wastewater, 100 ml of liquid fertilizer, and 300 ml of space blank. Anerobic reactor observed for 14 days with 5 observed parameters. Those parameters are TSS, VSS, COD, N, and P. After 14 days, added of Gandasil B fertilizer decreased the concentration of TSS, VSS, COD, N, and P amounted to 57,56%, 26,84%, 80,08%, 96,75%, and 93,18%. compared to the 0th day. While the addition of Gandasil D fertilizer decreased the parameters amounted to 3,48%, 6,21%, 86,72%, 58,10%, and 90,78%. In conclusion, Gandasil B fertilizer in the anaerobic reactor improves the rate of organic material degradation more effectively than Gandasil D fertilizer.Keywords - Anaerobic reactor, COD, fertilizer, N, nutrient, P, TSS, VSS
KANDUNGAN LOGAM NON ESENSIAL (Pb, Cd dan Hg) DAN LOGAM ESENSIAL (Cu, Cr dan Zn) PADA SEDIMEN DI PERAIRAN TUBAN GRESIK DAN SAMPANG JAWA TIMUR Moch. Syaifullah; Yuniar Ade Candra; Agoes Soegianto; Bambang Irawan
Jurnal Kelautan Vol 11, No 1 (2018)
Publisher : Department of Marine Sciences, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jk.v11i1.4497

Abstract

Logam berat seperti Pb, Cd, Hg, Cu, Cr dan Zn diperairan akan turun kemudian mengendap membentuk sedimen, beberapa organisme yang mencari makan didasar perairan akan mempunyai peluang besar untuk terpapar logam berat ini. Penelitian ini bertujuan untuk mengetahui kandungan logam berat non esensial Pb, Cd, Hg dan logam berat esensial Cu, Cr, Zn pada sedimen di perairan Tuban, Gresik dan Sampang. Kandungan logam berat pada sedimen ini menggunakan Atomic Absorption Spectrometry (AAS). Hasil penelitian menunjukkan bahwa kandungan logam non esensial timbal (Pb) yakni 3,3687 ± 0,5257 mg/kg-1 di Gresik, 2,9443 ± 0,3608 mg/kg-1 di Tuban dan 3,4253 ± 0,3697 mg/kg-1 di Sampang. Kandungan Kadmium (Cd) 2,978 ± 0,224 mg kg-1 di Tuban, 2,955 ± 0,179 mg kg-1 di Gresik, 3,012 ± 0,148 mg kg-1 di Sampang. Kandungan merkuri (Hg) 0,000029 ± 0,000012 mg kg-1 di Tuban, 0,000033 ± 0,000012 mg kg-1 di Gresik, 0,000034 ± 0,000045 mg kg-1 di Sampang. Adapun hasil analisis Kandungan logam esensial. Kandungan Tembaga (Cu) 0,0780 ± 0,0069 mg/kg-1 di Tuban, 0,0740 ± 0,0118 mg/kg-1 di Gresik dan 0,0750 ± 0,0069 mg/kg-1 di Sampang. Kandungan Kromium (Cr) 0,000033 ± 0,0000045 mg/kg-1 di Tuban, 0,000034 ± 0,0000011 mg/kg-1, 0,000024 ± 0,000009 mg/kg-1 di Sampang. Kandungan seng (Zn) 6,983 ± 0,278 mg kg-1 di Tuban, 5,873 ± 0,233 mg kg-1 di Gresik, 5,172 ± 0,233 mg kg-1 di Sampang.Kata kunci: logam non esensial, logam esensial, sedimenNON-ESSENTIAL METAL CONTENT (PB, CD AND HG) AND ESSENTIAL METALS (CU, CR AND ZN) IN SEDIMENTS IN THE WATERS OF TUBAN GRESIK AND SAMPANG, EAST JAVAABSTRACTHeavy metals such as Pb, Cd, Hg, Cu, Cr and Zn in the water will drop then settle to form sediments, some organisms that feed on the basis of water will have a great chance of being exposed to this heavy metal. This study aims to determine the content of non-essential heavy metals Pb, Cd, Hg and essential heavy metals Cu, Cr, Zn in sediments in the waters of Tuban, Gresik and Sampang. The heavy metal content in this sediment uses Atomic Absorption Spectrometry (AAS). The results showed non-essential metal content of lead (Pb) was 3.3687 ± 0.5257 mg / kg-1 in Gresik, 2.9443 ± 0.3608 mg / kg-1 in Tuban and 3.4253 ± 0.3697 mg / kg-1 in Sampang. The content of Cadmium (Cd) is 2.978 ± 0.224 mg kg-1 in Tuban, 2.955 ± 0.179 mg kg-1 in Gresik, 3.012 ± 0.148 mg kg-1 in Sampang. The content of mercury (Hg) is 0.000029 ± 0.000012 mg kg-1 in Tuban, 0.000033 ± 0.000012 mg kg-1 in Gresik, 0,000034 ± 0,000045 mg kg-1 in Sampang. The results of the analysis of the content of essential metals. Copper (Cu) content 0.0780 ± 0.0069 mg / kg-1 in Tuban, 0.0740 ± 0.0118 mg / kg-1 in Gresik and 0.0750 ± 0.0069 mg / kg-1 in Sampang. Chromium content (Cr) 0,000033 ± 0,0000045 mg / kg-1 in Tuban, 0,000034 ± 0,0000011 mg / kg-1, 0,000024 ± 0,000009 mg / kg-1 in Sampang. The zinc content (Zn) is 6.983 ± 0.278 mg kg-1 in Tuban, 5.873 ± 0.233 mg kg-1 in Gresik, 5.172 ± 0.233 mg kg-1 in Sampang.Keywords: non-essential metals, essential metals, sediments
Daya Dukung dan Laju Pertumbuhan Microcystis Hasil Isolasi dari Waduk Sutami pada Berbagai Variasi Konsentrasi Nitrat dan Fosfat dalam Medium Selektif B-12 Catur Retnaningdyah; Suharjono Suharjono; Agoes Soegianto; Bambang Irawan
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2590

Abstract

The main objective of this research was to calculate the carrying capacity and growth rate ofisolated Microcystis result in Sutami reservoir on a variety of nitrate and phosphateconcentrations in the B-12 selective medium. Research was conducted in the laboratory withpure experiments using completely randomized factorial design with factors of nitrateconcentration variation (8, 16, 32, and 64 ppm) and phosphate (0.2, 0.4, 0.8 and 1.6 ppm) in B-12medium. Repetition of the study was conducted three times at the same time. Microcystispopulation abundance which was counted every day until the stationary phase (day +30) wasused to calculate the rate of growth (β) and maximum abundance of Microcystis can besupported by each medium treatment (γ). The results showed that the growth rate of Microcystiswas not significantly influenced by levels of phosphate in the medium but significantly positivelycorrelated with increasing nitrate concentration in the medium. Carrying capacity or themaximum abundance (γ) of Microcystis was influenced by the combination of nitrate andphosphate in the B12 medium. Concentration of phosphate 0.4 ppm in medium combined withnitrate 8−64 ppm could support the highest abundance of Microcystis.
EFFECT OF SAPONIN-PODS EXTRACT ACACIA (ACACIA MANGIUM) TO HEMATOCRIT, HEMOGLOBIN AT TILAPIA (OREOCHROMIS NILOTICUS) Is Yuniar; Win Darmanto; Agoes Soegianto
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Problem of aquaculture shrimp, one of which is wild fish, competitor. Usually to eliminate the use of saponin from tea seed. saponin to kill fish competitors, (tilapia) are entered when filling water. Saponin to kill fish competitors, (tilapia) are entered when filling water. Saponin used from tea seed meal is imported. Indonesia is rich in biological resources of plants / substances that secondary metabolites naturally untapped optimal. The study was conducted in a laboratory scale with a completely randomized factorial design, using a series of six concentrations and three replicates for each species. LC50 analysis using probit analysis. Unfortunately, there was no scientific information for it. It has been done a laboratory work to study chemical compounds of pod Acacia (Acacia mangium) by phytochemical screening and measure those quantitatively. The result of these were that pod Acacia (Acacia mangium) contain alkaloid, flavonoid, tanin, saponin, steroid, and terpenoid. The results of this study indicate LC50-96h values for Pod Acacia mangium at salinity 0, 5, 10 and 15 promil is 5.487 ppm, 4.313 ppm, 3.985 ppm and 2.944. Value hemoglobin at 0promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 11.353±0.63)g/dL,10.607±0.18g/dL, 9.860±0.36 g/dL and 8.80±0.60. Value hemoglobin at 5promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 8.86±1.02 g/dL,7.76±0.943 g/dL, 7.42±0.71 g/dL and 4.68±0.53g/dL. Value hemoglobin at 10 promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 7.34±1.95 g/dL,6.07±0.755 g/dL, 5.23±1.34 g/dL and 3.23±035 g/dL. Value hemoglobin at 15promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 8.570±0.478 g/dL,5.18±0.876 g/dL, 3.42±0386 g/dL and 3.817±0.355g/dL. Value hematocrit at 0promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 35.033±3.32g/dL, 31.2±0.90 g/dL, 29.367±0.97 g/dL and26.60±1.345. Value hematocrit at 5promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 25.633±1.24 g/dL, 23.90±2.68 g/dL, 18.367±2.554 g/dL and 12.867±2.38 g/dL. Value hematocrit at 10 promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 21.567±6.134 g/dL,18.567±1.48 g/dL,14.767±3.46 g/dL and 10.53±3.38 g/dL. Value hematocrit at 15promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 26.100±1.42 g/dL, 15.667±2.79 g/dL,9.933± 2.04 g/dL and 11.933±1.19 g/dL. Saponin of acacia may lower hematocrit and hemoglobin an indication of damage to the red blood cells occurs hemolysis.
Analysis of White Feces Disease (WFD) caused by Vibrio sp. Bacteria and Dinoflagellata in Vannamei Shrimp (Litopenaeus vannamei) in Brackishwater Culture Pond Moh. Awaludin Adam; Irawati Mei Widiastuti; Ernawati Ernawati; Achmad Yani Yayan; Era Insivitawati; Yuliana Yuliana; Rini Fitriasari Pakaya; Agoes Soegianto; Ach. Khumaidi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 1 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i1.26684

Abstract

Highlight ResearchIndicated that white feces at Vanammei appeared along with the appearance of the disease besides its main trigger that is Vibrio sp.Increase the availability of carbon for photosynthesis process of phytoplankton.Clinical symptom of shrimp attacked by white feces disease is indicated by a change in intestine.AbstractShrimp disease that currently causes economic loss to shrimp farmers is White Feces Disease (WFD). This disease appeared due to several factors, such as poor pond management, unhealthy shrimp seed, and poor water quality which resulted in the appearance of Vibrio sp. bacteria and Dinoflagellate. This study aimed to analyze the cause of WFD outbreak in vannamei shrimp pond. The study method through direct experiment was applied in shrimp pond. Sampling was performed three times in each feeding tray to collect ten shrimps. Overall, sampling was performed twice a week. The Sample Survey Method was used to collect sample in this study. Result of study showed that clinical symptom was observed through changes in pattern and behavior of vannamei shrimp during culture. However, this observation resulted in insignificant data. Vannamei shrimp infected WFD tended to swim slower and often rose to the surface with body color turned red. Moreover, total organic matter (TOM) increased on week-7 along with the increasing growth of plankton, particularly from the Dinoflagellate group. However, bacterial growth of Vibrio sp. on week-7 was insignificant, yet many shrimps were found dead with white feces during that period. The result of analysis indicated that white feces disease was caused by Dinoflagellate besides the main trigger, namely, Vibrio sp.