Lisa Soegianto
Fakultas Farmasi Unika Widya Mandala Surabaya

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Uji Aktivitas Antibakteri dan Antikuorum Sensing Fraksi dari Ekstrak Etanol Daun Salam (Eugenia polyantha Wight.) Ardani, Yonas Bianityo; Soegianto, Lisa; Wijaya, Sumi
Jurnal Farmasi Sains dan Terapan Vol 1, No 1 (2013)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1153.419 KB) | DOI: 10.33508/jfst.v1i1.2343

Abstract

Eugenia polyantha is being used as empirical plant for antiseptic, anthelmintic, anesthetic, keratolytic and also has properties protein in bacterial cells. The antibacterial activity of extract of Eugenia polyantha has been proved in previous study, therefore this research was conducted to know the antibacterial and antiquorum sensing from fractions of ethanolic extract of Eugenia polyantha. The extraction was prepared by maceration and fraction was prepared by liquid-liquid fractionation method with n-hexane (non polar), ethyl acetate (semipolar) and water (polar). Bioautography method with thin layer chromatography and chloroform as mobile phase was conducted to determine the active fraction. The diameter of inhibition zone was measured or determined by diffusion method, while concentration of minimum inhibition (MIC) and bactericidal concentration (MBC) were determined using broth and agar dilution methods. Antiquorum sensing activity for P. aeruginosa was carried on, using pigment survey and motility method~ The antibacterial activities showed that ethyl acetate fraction 2596 gave diameter of growth inhibition zone greater than others, 1:1.48 mm against S. aureus and 9.03 mm against P. aeruginosa. The MIC of ethyl acetate fraction gave value of :1.56% against S. aureus and 12.5% against P. Aeruginosa, meanwhile the MBC was 3.1396 against S. aureus and more than 12.5% against P. aeruginosa. The result showed that ethyl acetate fraction has potential antiquorum sensing activity on concentration of 2.5 %. The active fraction of ethanolic extract of Eugenia polyantha which has antibacterial and antiquorum sensing activity was ethyl acetate fraction.
Effect of Various Concentration of Vegetable Protein in Hair Mask on The Hair Texture Helen, Ivony; Hadinoto, Idajani; Hadisoewignyo, Lannie; Soegianto, Lisa
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Effect of various concentrations of vegetable protein in hair mask on the hair texture including hair smoothness, hair shining, hair strength, and morphology of hair had been studied. Concentrations of vegetable protein are made 5%, 7.5%, and 10%. The hair mask was evaluated such as organoleptic, pH, viscosity, and allergy test. Hair mask used in six subjects that have damage hair for 8 times every 2 days. Then, the hair mask was evaluated such as hair smooth and hair shine that used tree trained panel and analyzed using non parametric method Q Cochran. Hair strength was evaluated using autograph  then  the  results  were    analyzes  using  ANOVA  test  (p<0.05),  while  hair morphology  was  evaluated  using  scanning  microscope  electron  (SEM).  The experimental  results  showed  that  vegetable  protein  in  hair  mask  increased  hair smoothness, hair strength, and morphology of damaged hair but did not give effect for hair shining. Various concentrations of vegetable protein (5%, 7.5%, and 10%) on hair mask given the same effect in hair texture (hair smoothness, hair strength, and hair morphology).
Perbandingan Antibakteri dari Ekstrak Etanol dan Fraksi Ekstrak Etanol Tanaman Ceguk (Quisqualis indica L.) Terhadap Staphylococcus aureus dan Escherichia coli Valeri, Sepvan; Soegianto, Lisa; Wijaya, Sumi
Jurnal Farmasi Sains dan Terapan Vol 2, No 2 (2015)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1033.663 KB) | DOI: 10.33508/jfst.v2i2.722

Abstract

Tanaman Ceguk merupakan tanaman yang berasal dari Myanmar dan Malaysia. Tanaman ini memiliki beberapa efek farmakologi antara lain immunomodulator, antihiperlipidemia, antipiretik, antioksidan dan antibakteri. Aktivitas antibakteri sebelumnya telah diteliti pada bagian bunga. Pada penelitian ini ekstrak etanol dan fraksi (n-heksana, etil asetat dan air) dari daun tanaman ceguk diuji aktivitas antibakterinya terhadap Staphylococcus aureus dan Escherichia coli. Fraksi aktif terpilih ditentukan dengan bioautografi kontak. Pengujian aktivitas antibakteri meliputi KHM (Kadar Hambat Minimum) dan KBM (Kadar Bunuh Minimum) dilakukan dengan metode mikrodilusi dan trifenil tetrazolium klorida 1% sebagai indikator visual. Golongan senyawa fraksi aktif ditentukan dengan uji kualitatif KLT dengan menggunakan penampak noda. Ekstrak dan fraksi n-heksana tanaman ini menghambat pertumbuhan pada bakteri Gram positif yaitu Staphylococcus aureus dengan nilai KHM 500 ppm pada fraksi n-heksana dan 1000 ppm untuk ekstrak. Ekstrak dan fraksi n-heksana tanaman ini tidak mampu membunuh Staphylococcus aureus dan Escherichia coli. Berdasarkan uji KLT, golongan senyawa fraksi aktif pada fraksi n-heksana yang diduga memiliki potensi sebagai antibakteri adalah golongan triterpenoid dengan harga Rf 0,63. Kata kunci : antibakteri, ceguk, fraksi, kadar hambat minimum, kadar bunuh minimum.
Uji Efek Sedasi dan Durasi Waktu Tidur Ekstrak Etanol Herba Putri Malu (Mimosa microphylla D.) pada Mencit (Mus musculus) Galur Swiss Webster Muliadi, Yeremia Kevin; Tamayanti, Wahyu Dewi; Soegianto, Lisa
Jurnal Farmasi Sains dan Terapan Vol 2, No 2 (2015)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1098.919 KB) | DOI: 10.33508/jfst.v2i2.719

Abstract

Sejak dahulu tanaman Putri Malu banyak digunakan oleh masyarakat sebagai obat batu ginjal, radang saluran nafas, anti mikroba, peluruh dahak, antipiretik, antiinflamasi, diuretik. Namun, tanaman Putri Malu dengan jenis spesies Mimosa microphylla D. masih jarang dilakukan penelitian serta masih sedikit literatur yang ada, terutama aktivitasnya dalam menimbulkan efek sedasi bila dibandingkan dengan tanaman Putri Malu dari spesies Mimosa pudica L. Oleh sebab itu dilakukan penelitian untuk menguji efek sedasi ekstrak herba Putri Malu (Mimosa microphylla D.) terhadap mencit (Mus musculus) galur Swiss webster untuk mengetahui potensi sedatifnya. Herba Putri Malu Mimosa microphylla D. diekstraksi dengan cara maserasi dengan etanol 96% dan diberikan kepada mencit jantan galur Swiss webster sebanyak 25 ekor yang dibagi dalam 5 kelompok dengan dosis pemberian 600 mg/kgBB, 1200 mg/kgBB, dan 2400 mg/kgBB masing-masing 1 ml/20 gBB. Penelitian ini dilakukan dengan berbagai metode: holeboard, evasion box, platform, rotarod untuk melakukan uji efek sedasi, uji induksi mula tidur, dan uji durasi waktu tidur, serta sebagai pembanding digunakan fenobarbital 30 mg/kgBB. Dari hasil percobaan uji sedatif, diketahui bahwa dosis 600 mg/kgBB ekstrak etanol herba Putri Malu (Mimosa microphylla D.) memiliki aktivitas sedatif terbaik dan pemberian pada 1200 mg/kgBB mampu memperpanjang durasi tidur mencit, namun ekstrak etanol herba Putri Malu (Mimosa microphylla D.) tidak mampu memperpendek waktu mula tidur mencit. Ekstrak etanol herba Putri Malu (Mimosa microphylla D.) memiliki aktivitas dalam menimbulkan efek sedatif, memperpanjang durasi waktu tidur, namun tidak mampu memperpendek waktu mula tidur mencit. Kata kunci: Mimosa microphylla D., efek sedatif, durasi waktu tidur, waktu mula tidur.
Pengaruh Pemberian Infus Kelopak Kering Rosella (Hibiscus sabdariffa) Terhadap Kadar Kolesterol Total Serum Darah Tikus Hiperkolesterolemia Octavia, Stefani Kartika; Surdijati, Siti; Soegianto, Lisa
Jurnal Farmasi Sains dan Terapan Vol 2, No 2 (2015)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (939.618 KB) | DOI: 10.33508/jfst.v2i2.715

Abstract

Rosella (Hibiscus sabdariffa) merupakan salah satu tanaman bermanfaat sebagai bahan makanan, minuman maupun bahan obat. Kelopak Rosella berkhasiat sebagai antihipertensi, antidiabetes, antikolesterol, terapi gangguan liver dan asam urat. Penelitian ini bertujuan untuk mengetahui apakah ada pengaruh yang signifikan dari pemberian infus kelopak kering Rosella dalam menurunkan kadar kolesterol total serum darah tikus hiperkolesterolemia. Penelitian ini menggunakan 25 tikus putih jantan galur Wistar yang diberi propiltiourasil 0,01% dan makanan tinggi kolesterol selama 2 minggu. Kemudian dibagi dalam lima kelompok, yaitu kontrol (PGA 3%), pembanding (Simvastatin 0,9 mg/kgBB) dan 3 kelompok uji yang diberi infus Rosella dosis 125 mg/kgBB, 250 mg/kgBB dan 500 mg/kgBB, secara oral selama 2 minggu. Data dianalisis dengan uji statistik Anova dilanjutkan uji Tukey HSD 5%. Perhitungan korelasi digunakan untuk mengetahui hubungan antara peningkatan dosis dengan penurunan kolesterol total. Tidak ada perbedaan bermakna pada setiap pasangan kelompok dengan signifikansi >0,05 untuk semua kelompok kecuali antara K1 dan K5 (signifikansi
Karakterisasi dan Uji Aktivitas Antibakteri dari Fungi Endofit Ranting Tanaman Manggis (Garcinia mangostana L.) terhadap Escherichia coli dan Staphylococcus aureus Widjaja, Kevin; Soegianto, Lisa; Ervina, Martha
Jurnal Farmasi Sains dan Terapan Vol 6, No 1 (2019)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2304.672 KB) | DOI: 10.33508/jfst.v6i1.2007

Abstract

Resistance is commonly caused by improper usage of antibiotics, so the treatment of the infection always needs a new and more potent compounds. Endophytic microbes are microbes that all or part of its life stay in the tissues of plant, which established a mutually beneficial relationship between them. In this research, isolation of endophytic fungi was conducted by sticking the sterilized twigs of mangosteen to the media Malt Extract Agar obtained three pure endophytic fungi colonies. The antibacterial activity assay with direct inoculation method at media Plate Count Agar which was inoculated with bacteria and showed that the antibacterial activity against Escherichia coli ATCC 8739 was one isolate (ER2) with inhibition average ratio 1.42 and against Staphylococcus aureus ATCC 6538 was three isolates (ER1, ER2, and ER3) with inhibition average ratio 1.66; 1.37; and 1.89. The fungus were observed characteristics of the macroscopic, microscopic and biochemical assay. ER1 and ER3 isolates are suspected as a Penicillium genus with specific characteristics such as the conidiophore, fialid, metulae, and conidiospore. ER2 isolate was suspected as a Rhizoctonia genus because there are nearly right-angled hyphae and monilioid cells.
Uji Aktivitas Antimikroba Ekstrak Etanol Daun Cayratia trifolia terhadap Staphylococcus aureus dan Candida albicans Sari, Rizki Sari Endah; Soegianto, Lisa; Hermanu, Liliek S,
Jurnal Farmasi Sains dan Terapan Vol 5, No 1 (2018)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.719 KB) | DOI: 10.33508/jfst.v5i1.2052

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Cayratia trifolia is a wild plant that is easily found in the lowlands, tropical and subtropical regions. Cayratia trifolia is known to be efficacious as an antibacterial, antifungal, antiprotozoa, hypoglycemia, anticancer and diuretic. This study aims to determine whether the ethanol extract of Cayratia trifolia leaves has antimicrobial activity against Candida albicans and Staphylococcus aureus using well diffusion method; to determine the value of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) using microdilution method; and to determine the secondary metabolite groups which have antimicrobial activity against Staphylococcus aureus and Candida albicans. In this study bioautography method was carried out to determine the secondary metabolite group that can inhibit microbial growth. Ethanol extract of Cayratia trifolia (Galing-galing) leaves has no antimicrobial activity against Candida albicans. The MIC value of the ethanol extract of Cayratia trifolia (Galing-galing) leaves against Staphylococcus aureus is at a concentration of 125 ppm, while the MBCvalue is at a concentration of 250 ppm. The group of compounds that have antibacterial activity against Staphylococcus aureus is polyphenol, meanwhile flavonoid suspected has activity against Candida albicans.
Studi pendahuluan: Korelasi Aktivitas Antikolesterol dengan Aktivitas Antioksidan Ekstrak Etanol Daun Salam (Syzygium polyanthum) Wijaya, Sumi; Yonas, Stefani Maureen Kasih; Hartanti, Lanny; Setiawan, Henry K.; Soegianto, Lisa
Jurnal Farmasi Sains dan Terapan Vol 5, No 2 (2018)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2982.965 KB) | DOI: 10.33508/jfst.v5i2.2143

Abstract

Bay leaf leaves (Syzygium polyanthum) are known to have anti-cholesterol activity, with the mechanism inhibited HMG-CoA Reductase enzyme. The purpose of this study was to determine the correlation between anticholesterol activity of bay leaf ethanol extract obtained by percolation method and its antioxidant activity. The parameter used to measure anticolesterol activity is the IC50 value. Antioxidant activity was evaluated by DPPH and Frap methods. The results showed that the ethanolic extract of bay leaves had an IC50 value of 49.50 ± 0.70 ppm. Ethanol extract of bay leaf showed antioxidant activity with IC50 value of 888.0835 ppm and equality of values with 1 mM FeSO4 of 295 ppm. Based on the results of the analysis, the anticholesterol activity of Bay leaf ethanol extract in inhibiting the HMG-CoA Reductase enzyme did not have a linear correlation with its antioxidant activity.
Uji Potensi Antibakteri dan Antibiofilm Minyak Atsiri Umbi Teki (Cyperus rotundus L.) terhadap Staphylococcus aureus ATCC 6538 Uthari, Ni Made; Soegianto, Lisa; Hermanu, Liliek S.
Jurnal Farmasi Sains dan Terapan Vol 4, No 2 (2017)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1384.532 KB) | DOI: 10.33508/jfst.v4i2.2183

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Infectious disease is a disease that is caused by an invasion of pathogenic microorganisms in the body. Biofilm is a group of microorganisms that are able to differentiate and proliferate in the complex way as well as being able to communicate between cells through a matrix of polysaccharides. In chronic infection, biofilm has an important role to maintain the bacteria which can cause resistance to antibiotics. Teki (Cyperus rotundus L.) is one of the plants that has many benefits for different treatment. The aim of this study was to determine the antibacterial activity and inhibition of biofilm activity of essential oil of Cyperus rotundus L. tubers against Staphylococcus aureus. Essential oil was extracted by distillation using Stahl with water as the solvent and the yield of essential oil obtained was 0.22%. Determination of antibacterial activity and antibiofilm activity were carried out by microdilution method using microplate 96 U-PVC bottom wells against Staphylococcus aureus which is equivalent to the solution standard of ½ Mc Farland I. The results of antibacterial activity tests showed that MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) values were 0.05% (v/v) and 12.5% (v/v). Antibiofilm activity test showed that the essential oil of Cyperus rotundus L. tubers is able to inhibit 89.01% of the biofilm formation of Staphylococcus aureus on the concentration of 0.09% (v/v).
Isolasi dan Karakterisasi Fungi Endofit dari Daun Tanaman Bintaro (Cerbera odollam) yang Berpotensi sebagai Antibakteri Wonowijaya, Suwandi; Soegianto, Lisa
Jurnal Farmasi Sains dan Terapan Vol 5, No 2 (2018)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.53 KB) | DOI: 10.33508/jfst.v5i2.2144

Abstract

The need of antibiotics is increasing, spurred to find compounds that have antimicrobial activity from natural ingredients. One way to obtain antimicrobial compounds is through endophytic fungi. This study aimed to isolate and characterize endophytic fungi obtained from the leaves of the plant Bintaro (Cerbera odollam) that potential as an antibacterial against Staphylococcus aureus and Escherichia coli. Leaf surface was sterilized by soaking in 70% alcohol for 2 minutes, 5.3% sodium hypochlorite for 5 minutes, 70% alcohol for 1 minute, rinsed with sterile distilled water and dried using sterile wipes. Bintaro leaf that have been sterilized was puton Potato Dextrose Agar (PDA) and then incubated at room temperature for 7-14 days. The growing fungi was inoculated in Potato Dextrose Yeast (PDY) for 7-14 days at room temperature based on the macroscopic difference. Endophytic fungi that have been purein macroscopic way was tested antibacterial activity on Staphylococcus aureus and Escherichia coli and continued to macroscopic and microscopic observation. Obtained three types of endophytic fungi were isolated from bintaro leaves (Cerbera odollam) with the code EB 1, EB 2 and EB 3 that suspected from genus Fusarium, Geotrichum and Aspergillus. The antibacterial activity test show that results of three endophytic fungi obtained has no antibacterial activity against Staphylococcus aureus and Escherichia coli.