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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia Buletin Hama dan Penyakit Tumbuhan Agrikultura Jurnal Natur Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Hortikultura Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Agrotek Tropika JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Entomologi Indonesia Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Proceedings Series on Physical & Formal Sciences
SRI HENDRASTUTI HIDAYAT
Departemen Proteksi Tanaman, Fakultas Pertanian, IPB Jl. Kamper Kampus IPB Dramaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Veterinary

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Cover Jurnal Fitopatologi Vol. 18 No. 1, Januari 2022 Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 18 No 1 (2022)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.1.i

Abstract

This editorial contains the front cover, editorial page, and back cover of the Indonesian Journal of Phytopathology Vol. 18 No. 1, Januari 2022
Kemampuan Kolonisasi Cendawan Endofit dan Peningkatan Pertumbuhan Bibit Cabai Evan Purnama Ramdan; Efi Toding Tondok; Suryo Wiyono; Sri Hendrastuti Hidayat; Widodo Widodo
Proceedings Series on Physical & Formal Sciences Vol. 2 (2021): Prosiding Seminar Nasional Fakultas Pertanian dan Perikanan
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (395.798 KB) | DOI: 10.30595/pspfs.v2i.175

Abstract

Endophytic fungi are fungi that live in healthy plant tissues without causing disease symptoms. Endophytic fungi in chili have been tested as both biocontrol agents and growth promoters, but their colonization has not been reported. Therefore, this study aimed to determine the level of colonization of endophytic fungi and its effect on the growth of chili seedlings. A total of 8 endophytic fungi were prepared at a density of 2.8 × 106 CFU mL-1. Then the endophytic fungus was inoculated 2 times, first by soaking the seeds, and secondly by watering the endophytic fungus suspension on chili seedlings aged 3 weeks after sowing. Endophytic fungi were re-isolated on chili seedlings that were 4 weeks old after sowing on the roots and stems to determine their colonization ability. Chili seeds were then maintained for up to 4 weeks after transplanting to observe their growth. The results showed that the endophytic fungal colonization ranged from 26-60% on the chili root, while at the base of the stem it was 20-40% with a different pattern of colonization distribution. In addition, endophytic fungus colonization was also able to increase the shoot height and root length of chili seedlings.
Identification of mealybugs on Piper nigrum as vector of Piper yellow mottle virus (Badnavirus: Caulimoviridae) Miftakhurohmah Miftakhurohmah; Sri Hendrastuti Hidayat; Kikin Hamzah Mutaqin; Bonny Poernomo Wahyu Soekarno; Dono Wahyuno
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 2 (2022): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.222144-153

Abstract

Piper yellow mottle virus (PYMoV) is the dominant virus that causes mottle disease in black pepper (Piper nigrum). Two species of mealybugs, Ferrisia virgata and Planococcus minor have been reported as vectors of PYMoV. A different species of mealybug that has never been reported before was found in black pepper. Molecular approaches including total DNA isolation of mealybug collected from the field, mealybugs identification by DNA barcode, detection of PYMoV in single mealybugs, were conducted as an approach to identify the potential of mealybugs as PYMoV vector in the field. Mealybugs were collected from black pepper plants in Cimanggu (Bogor, West Java) and Sukamulya (Sukabumi, West Java). Characters of adult females were observed for morphological identification. Molecular-based identification of the mealybugs and PYMoV involved the following procedures: total DNA isolation, DNA amplification, nucleotide sequencing and sequence analysis. Three species of mealybugs, P. minor, F. virgata and Paracoccus marginatus were confirmed by morphological and molecular identification. This is the first report for the occurrence of P. marginatus in black pepper plants. PYMoV was successfully detected from field samples of F. virgata, P. minor and P. marginatus. This finding indicates the potential of insect vectors for disease spread and distribution.
Meristem Culture Method for Virus Elimination from Shallot Bulb Prabawati Hyunita Putri; Diny Dinarti; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 16 No 4 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.4.185-189

Abstract

Meristem Culture Method for Virus Elimination from Shallot Bulb Shallot farmers in Indonesia have commonly grown bulbs from the previous planting season as seed sources for the next season. This may cause the accumulation of viruses in bulbs which in turn lowering plant productivity. A research was conducted to optimize the meristem culture method combined with thermotherapy (30 ºC) to produce virus-free shallot bulbs. Shallot bulbs of cv. Bima Curut that had been confirmed infected by Potyvirus and Carlavirus were used as explant sources. Bulb sterilization was carried out using bactericides, fungicides, and sodium hypochlorite. Meristem shoots of 0.6 to 0.7 mm in size were isolated from bulbs and plant successively in the shoot, root, and bulb induction medium to form mini bulbs. The mini bulbs were then planted in the screen house for 2 to 2.5 months until micro bulbs were formed. Virus detection by reverse transcription-polymerase chain reaction method confirmed that plantlets at the multiplication stage and plants in the screen house were free of Potyvirus and Carlavirus infestations. This study showed that the meristem culture method combined with thermotherapy is a potential approach for producing virus-free shallot bulbs.
Identification of Phytoplasmas on Carrot (Daucus carota L.) and Leafhopper Associated with Yellow Disease in Bogor and Bandung, West Java Isti Wulandari; Kikin Hamzah Mutaqin; Giyanto Giyanto; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 16 No 4 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.4.157-165

Abstract

Gejala kuning mirip seperti infeksi fitoplasma ditemukan pada pertanaman wortel di Jawa Barat. Penelitian bertujuan mengidentifikasi fitoplasma pada tanaman wortel dan serangga wereng yang berasosiasi dengan gejala kuning di Bogor dan Bandung. Wereng yang ditemukan pada pertanaman wortel di Bogor terdiri atas 5 spesies wereng daun (Hemiptera: Cicadellidae), yaitu Balclutha incisa (Matsumura), Orosius argentatus (Evans), Cicadulina bipunctata (Matsumura), Empoascanara indica (Datta), Exitianus indicus (Distant), dan 1 spesies wereng batang (Hemiptera: Delphacidae), yaitu Sogatella furcifera (Horváth). Deteksi fitoplasma pada tanaman dan wereng dilakukan dengan nested-PCR, sikuensing, dan analisis DNA sikuen. Nested-PCR berhasil mengamplifikasi DNA target, yaitu gen 16S rRNA fitoplasma dari tanaman dan wereng. Analisis identitas matriks sikuen fitoplasma asal tanaman wortel di Bogor menunjukkan homologi tertinggi dengan fitoplasma dari grup 16SrII, yaitu Peanut witches’-broom phytoplasma; sedangkan sampel asal Bandung menunjukkan homologi tertinggi dengan fitoplasma dari grup 16SrI, yaitu Ca. Phytoplasma asteris dan Tomato big bud phytoplasma. Analisis identitas matriks sikuen fitoplasma asal wereng di Bogor (B. incisa, S. furcifera) menunjukkan homologi tertinggi dengan fitoplasma dari grup 16SrII, yaitu Cactus witches’-broom phytoplasma; sedangkan fitoplasma asal C. bipunctata dari Bogor menunjukkan homologi tertinggi dengan fitoplasma grup 16SrI, yaitu Ca. Phytoplasma asteris. Tulisan ini merupakan laporan pertama fitoplasma yang menginfeksi tanaman wortel di Bogor dan Bandung.
Insect Vector and Seedborne Transmission of Papaya ringspot virus Sri Hendrastuti Hidayat; Tutik Harmiyati; Abdul Muin Adnan
Jurnal Fitopatologi Indonesia Vol 18 No 3 (2022)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.3.101-106

Abstract

Penyakit bercak bercincin pada pepaya yang disebabkan oleh Papaya ringspot virus (PRSV) dilaporkan sudah menyebar luas di Indonesia. Pemencaran penyakit ini diketahui terjadi melalui bibit tanaman terinfeksi dan serangga vektor kutudaun. Penelitian dilakukan untuk menguji efisiensi penularan PRSV melalui dua spesies kutudaun, yaitu Aphis gossypii dan Myzus persicae dan membuktikan bahwa PRSV tidak dapat ditularkan melalui biji. Penularan PRSV isolat Medan melalui kutudaun dilakukan pada tanaman pepaya var. California dengan periode makan akuisisi dan periode makan inokulasi masing-masing selama 10 menit. Minimal 5 ekor A. gossypii dan 10 ekor M. persicae diperlukan untuk keberhasilan penularan PRSV. Penularan PRSV melalui A. gossypii menghasilkan insidensi penyakit yang lebih tinggi dan gejala penyakit yang lebih parah dibandingkan dengan penularan melalui M. persicae. Bibit pepaya dari biji yang berasal dari buah yang menunjukkan gejala bercak bercincin tidak menimbulkan gejala penyakit dan tidak diperoleh fragmen DNA spesifik PRSV pada deteksi PRSV menggunakan metode reverse transcription polymerase chain reaction. Hasil penelitian ini mengonfirmasi potensi kutudaun sebagai vektor PRSV dan membuktikan bahwa PRSV tidak bersifat tular biji.
Infrared Thermography for Early Detection of Pepper yellow leaf curl virus on Chili Plants Triyani Dumaria; Sri Hendrastuti Hidayat; Purnama Hidayat
Jurnal Fitopatologi Indonesia Vol 19 No 1 (2023)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.1.1-10

Abstract

Infrared Thermography for Early Detection of Pepper yellow leaf curl virus on Chili Plants Observations of plant pests and diseases are generally carried out by looking for visual symptoms for each disease target. Agricultural technology 4.0 began to be used for the development of plant disease detection methods. It was reported that there were differences in color and temperature between diseased and healthy plants which could be recorded by a thermal camera. This study aimed to determine the potential of the FLIR One Pro-IOS thermal camera to record differences in color and temperature between viral-infected and healthy chili plants. Chili plants in the greenhouse that were inoculated with Pepper yellow leaf curl virus (PYLCV) experienced an increase in temperature 3 days after inoculation (28.62 ℃) compared to plants that were not inoculated with PYLCV (27.32 ℃). Digital image recording of chili leaf samples from the field showed that plants infected by multiple viruses (Chilli veinal mottle virus, Pepper mottle virus and PYLCV) has higher temperature than those infected with a single virus. The lowest and highest mean temperatures were recorded in plant samples infected with PepMV (17.74 ℃) and mixed infected by PYLCV and ChiVMV (25.68 ℃). Digital images of virus-infected plants tend to show a predominance of bright yellow, while virus-free plants showed a predominance of dark purple. Further analysis confirmed higher digital numbers for diseased plant images than healthy plants. The thermography method has the potential to be an early detection method because it can detect viral infection before visual symptoms appear.
PENILAIAN KEPARAHAN GEJALA VIRUS PADA Capsicum frutescens BERBASIS INDEKS VEGETASI DAN PENGAMATAN VISUAL DI LAPANGAN Muhammad Taufik; Asmar Hasan; Sri Hendrastuti Hidayat; Ayu Kartini Parawansa; Arifin Tasrif
Jurnal Agrotek Tropika Vol 11, No 1 (2023): JURNAL AGROTEK TROPIKA VOL 11, FEBRUARI 2023
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v11i1.6063

Abstract

Infeksi virus dapat menyebabkan gejala yang bervariasi pada tanaman. Penilaian tingkat keparahan gejala virus tanaman di lapangan umumnya menggunakan metode konvensional berbasis pengamatan visual. Seiring dengan perkembangan teknologi digital saat ini, telah dikembangkan pula metode penilaian terkini berbasis analisis citra digital. Namun informasi terkait kepresisian dan keakurasian kedua metode penilaian tersebut masih sangat terbatas khususnya penilaian keparahan gejala yang disebabkan oleh virus. Penelitian ini bertujuan untuk mengevaluasi hasil penilaian keparahan penyakit berdasarkan metode konvensional dan analisis citra digital berbasis NDVI (Normalized Difference Vegetation Index) serta membandingkan akurasi kedua metode penilaian tersebut dalam mengestimasi variabel produksi tanaman. Survei lapangan dilakukan dengan melakukan perekaman citra dan pengamatan secara visual terhadap gejala serangan virus pada tajuk tanaman cabai. Dilanjutkan dengan pengukuran bobot buah sampel tanaman, dan analisis citra digital tanaman. Hasil penelitian menunjukkan bahwa penilaian keparahan gejala virus menggunakan metode analisis citra digital berbasis indeks vegetasi NDVI lebih presisi (standar deviasi < 0,04) dibandingkan metode pengamatan visual (standar deviasi > 4,00), khususnya pada fase generatif. Selain itu, metode indeks vegetasi NDVI juga lebih akurat dalam mengestimasi bobot buah cabai karena koefisien regresi bertanda positif dengan nilai R2 > 0,90.
Application of Guano Filtrate on Infection of Pepper yellow leaf curl virus in Chilli Plants Azmi Khoirin Nada; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 16 No 5 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.5.191–199

Abstract

Pepper yellow leaf curl virus (PYLCV) adalah anggota genus Begomovirus, yang menyebabkan penyakit daun keriting kuning pada tanaman cabai di Indonesia. Virus ini ditularkan oleh vektor Bemisia tabaci (Hemiptera: Aleyrodidae). Penelitian dilakukan untuk mengetahui potensi guano dalam menekan penyakit daun keriting kuning pada cabai. Percobaan lapangan dilakukan menggunakan rancangan faktorial dalam rancangan acak kelompok dengan 2 faktor. Faktor pertama adalah kultivar cabai (‘Gelora’, ‘Bara’, dan ‘Pelita 8’) dan faktor kedua adalah perlakuan filtrat guano (sebelum inokulasi virus, 1 minggu setelah inokulasi virus, 2 minggu setelah inokulasi virus, inokulasi virus tanpa guano, dan tanpa inokulasi virus atau guano). Inokulasi virus dilakukan menggunakan B. tabaci. Secara umum, gejala muncul 1 hingga 3 minggu setelah inokulasi meskipun periode inkubasi bervariasi antara kultivar cabai. Gejala mosaik hijau dengan daun keriting sebagian besar ditemukan pada ‘Gelora’, sedangkan gejala menguning dengan daun melengkung ke atas sebagian besar ditemukan pada ‘Bara’ dan ‘Pelita 8’. Infeksi Begomovirus pada tanaman yang menunjukkan gejala telah dikonfirmasi melalui metode polymerase chain reaction. Perlakuan guano tidak menyebabkan penghambatan pada insidensi dan keparahan penyakit; demikian juga, tidak memengaruhi tinggi tanaman dan periode berbunga.
Intensity of Main Disease in Several Superior Sugarcane Clones at Krebet Baru Sugar Factory, Malang Sakinah Inayatur Rizqiyah; Titiek Yulianti; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 18 No 6 (2022)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.6.231-238

Abstract

Tebu (Saccharum officinarum) merupakan tanaman perkebunan penting sebagai penghasil utama gula di Indonesia. Penurunan produksi gula tebu dalam beberapa tahun terakhir tidak sejalan dengan permintaan gula tebu yang kian meningkat. Pemuliaan tanaman diarahkan untuk mendapatkan varietas-varietas tebu unggul yang diharapkan memiliki produktivitas tinggi dan tahan terhadap faktor-faktor gangguan yang dapat menurunkan produktivitas tanaman. Pengamatan penyakit mosaik bergaris (Sugarcane streak mosaic virus/SCSMV), pokahbung (Fusarium moniliforme), dan luka api (Sporisorium scitamineum) dilakukan pada 14 klon tebu unggul di PG Krebet Baru, Malang. Pengamatan intensitas penyakit dilakukan setiap 2 minggu selama 3 bulan dan sampel tanaman yang menunjukkan gejala penyakit dideteksi penyebab penyakitnya di laboratorium. Gejala penyakit mosaik bergaris terjadi pada tiga klon tebu dengan intensitas antara 8.33% dan 63.89%; sedangkan gejala penyakit pokahbung dan luka api ditemukan pada 11 dan 14 klon tebu dengan intensitas berturut-turut 2.78% sampai 22.22% dan 11.11% sampai 25%. Konfirmasi keberadaan SCSMV diperiksa dengan metode polymerase chain reaction; sedangkan cendawan F. moniliforme dan S. scitamineum melalui isolasi jaringan dan pengamatan jaringan meristem. Secara umum insidensi penyakit tergolong rendah pada klon 8 dan 12 sehingga klon tersebut dapat direkomendasikan sebagai klon potensial yang digunakan dalam mengendalikan penyakit utama tebu.
Co-Authors . SUDARSONO Abdul Muin Adnan ALI NURMANSYAH Amelia Feryna Bulan Dini Ana Septiana Saputri Anas Dinurrohman Susila Aqlima , Aqlima, nFN Arifin Tasrif Asmar Hasan Asniwita Asniwita Astri Windia Wulandari Wulandari ATI SRI DURIAT AUNU RAUF Ayu Kartini Parawansa Azmi Khoirin Nada Bambang S. Purwoko Bambang Sapta Purwoko Bonny Poernomo Wahyu Soekarno Bonny Poernomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Budi Tjahjono Darni Rambu D. Siala Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Diny Dinarti Diny Dinarti Dono Wahyuno Dono Wahyuno DWI SUBEKTI Dwi Subekti Dwi Wiyati Nurul Septariani Dwiwiyati Nurul Septariani Efendi, Darda Efi Toding Tondok Efi Toding Tondok Eliza Suryati Rusli Endang Nurhayati Endang Nurhayati Erika Rosminim Purba Evan P. Ramdan Evan Purnama Ramdan Evan Purnama Ramdan Evan Purnama Ramdan Gede Suastika GEDE SUASTIKA Gede Suastika Gede Suastika Gede Suastika GEDE SUASTIKA Giyanto Giyanto Hamdayanty Hamdayanty Hari Priwiratama Harwan Susetio Heri Harti Ifa Manzila Ifa Manzila Ika Mariska Ika Mariska Irsan Nuhantoro Ishak Manti ISHAK MANTI Isti Wulandari Jamsari Jamsari Jati Adiputra John Thomas Jumanto Harjosudarmo Jumanto Harjosudarmo JUMANTO HARJOSUDARMO Jumsu Trisno Kadwati Kadwati Kikin H Mutaqin KIKIN HAMZAH MUTAQIN Ladja, Fausiah T. Laksono Trisnantoro Listihani, Listihani Meity S Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Melinda . Memen Surahman Miftakhurohmah Miftakhurohmah Miftakhurohmah Mimi Sutrawati Muhamad Syukur Muhammad Herman Muhammad Taufik MUHAMMAD TAUFIK Naimatul Farida Neni Gunaeni Nissa Fawwaz Adilah NOOR AIDAWATI ORAWAN CHATCHAWANKAN PANICH Prabawati Hyunita Putri Puji Lestari Purnama Hidayat PURNAMA HIDAYAT Purwoko, Bambang Sapto Purwono Purwono Rahayuwati, Sat Rahmi Yunianti Rahmi Yunianti Redy Gaswanto Redy Gaswanto, Redy Reymas M.R. Ruimassa Rina Rachmawati Rita Noveriza Rita Noveriza Rita Noveriza RITA NOVERIZA Rokhana Faizah Roy Ibrahim Rusmilah Suseno RUSMILAH SUSENO Rustiani, Ummu S. Saiful Akhyar Lubis Sakinah Inayatur Rizqiyah Sari Nurulita Sarsidi S astrosumarjo Sarsidi Sastrosumarjo Sarsidi Sastrosumarjo Sherli Anggraini Sientje Mandang Sumaraw Siti Hafsah Siti Shofiya Nasution Slamet Susanto Sobir Sobir Sobir Sobir Soemartono Sosromarsono Soemartono Sosromarsono Sri Hartati Sri Hartati Sri Hartati Sri Sulandari Sri Sulandari Sri Sulandari Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Suryo Wiyono Susanti Mugi Lestari Susanti Mugi Lestari Taufik, Muh Tega Kintasari Titiek Yulianti TRI ASMIRA DAMAYANTI TRI JOKO SANTOSO Trimuri Habazar TRIMURTI HABAZAR Triyani Dumaria Tutik Harmiyati Ummu S. Rustiani Ummu Salamah Rustiani UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Utomo Kartosuwondo Utomo Kartosuwondo UTOMO KARTOSUWONDO Vinsen Willi Wardhana Wayan Winasa Widodo Widodo . Widodo Widodo Widodo Widodo Widodo Widodo Willing Bagariang Yudia Nurhaelena Zahratul Millah