Pudji Srianto
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Forty healthy and non-pregnant Fries Holland dairy cows which had received oestrus synchronization were divided randomly into four experimental groups. Artificial inseminations (AI) were implemented 8-10 hours post estrous using frozen semen from the same breed. The first three groups were injected with 100, 150 and 200 mg medroxy progesterone acetate (MPA) i.m., respectively, three days post AI, while the other group was injected with placebo as control group. Madura cattle embryos were transfe Imam Mustofa; Laba Mahaputra; Pudji Srianto; Suzanita Utama
Media Veteriner Vol. 6 No. 1 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

Forty healthy and non-pregnant Fries Holland dairy cows which had received oestrus synchronization were divided randomly into four experimental groups. Artificial inseminations (AI) were implemented 8-10 hours post estrous using frozen semen from the same breed. The first three groups were injected with 100, 150 and 200 mg medroxy progesterone acetate (MPA) i.m., respectively, three days post AI, while the other group was injected with placebo as control group. Madura cattle embryos were transfered contralaterally seven days post AI. The percentage of pregnancy in groups which had received 100 and 150 mg MPA was 37.5%r, and in group received 200 mg MPA and control was 55.6%, and 60%, (P>0.05), respectively. Calving percentages of the 100, 150, 200 mg MPA and the control groups were 25,37.5,55.6 and 60% respectively (P>0.05). All birth gave single calve.
Peningkatan Ekspresi Vascular Endothel Growth Factor dan Mitogen Activating Protein Kinase Plasenta Tikus yang Dipapar Carbon Black ((INCREASING EXPRESSION ON VASCULAR ENDOTHELIAL GROWTH FACTOR AND MITOGEN ACTIVATING PROTEIN KINASE IN PLACENTA RATS EXPOSE Viski Fitri Hendrawan; Widjiati Widjiati; Suherni Susilowati; Pudji Srianto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.745 KB) | DOI: 10.19087/jveteriner.2017.18.1.59

Abstract

Carbon black is one of the components of particulate matter (PM), known as cytotoxic, causing inflammationin the respiratory and cardiovascular system, and able to pass through the placental barrier, which may affect the fetus. The effects caused by pollutants depend on the intensity and time length of the exposure.The objective of this study is to identify Vascular Endothelial Growth Factor (VEGF) and Mitogen Activating Protein Kinase (MAPK) as an indicator of maternal placental inflammation in pregnant rats (Rattus norvegicus) exposed to carbon black with immunohistochemical technique. This study was a laboratory experiment with three treatment groups and seven replications using pregnant female white rats (Rattus norvegicus). Pregnant female rats were exposed to carbon black with a dosage of 523 mg/m3 and 1064 mg/m3 during pregnancy of day 6 up to day 11. The next phase was the observation and examination of the expressions of VEGF and MAPK by immunohistochemical staining toward placenta of the white rats exposed to carbon black. The result of the study showed an increase in the expression of VEGF in the placenta of the rats exposed to carbon black with a dosage of 523 mg/m3 and 1064 mg/m3 per inhale during the mid-period (6-11) of pregnancy. In addition, there was also an increasein the expression of MAPK on the placenta of the rats exposed to carbon black with a dosage of 523 mg/m3and 1064 mg/m3 per inhale during the mid-period (day 6-11) of pregnancy. In conclusion, the expression of VEGF and MAPK are increased along with the increased dosage of carbon black and age of gestation. ABSTRAK Carbon black merupakan salah satu komponen partikulat matter (PM) yang diketahui bersifat sitotoksik serta menyebabkan terjadinya inflamasi pada sistem pernapasan dan kardiovaskular serta dapat melewati barier plasenta fetus sehingga dapat memengaruhi fetus. Efek yang ditimbulkan oleh polutan tergantung dari besarnya paparan dan lama waktu paparan. Penelitian ini bertujuan untuk mengidentifikasi Vascular Endothel Growth Factor (VEGF) dan Mitogen Activating Protein Kinase (MAPK) sebagai indikator imflamasi pada plasenta maternal tikus (Rattus norvegicus) yang dipapar carbon black dengan teknik imunohistokimia. Penelitian ini dilakukan dengan tiga kelompok perlakuan dan tujuh kali ulangan yang menggunakan tikus betina bunting. Tikus betina bunting dipapar dengan carbon black dosis 523 mg/m3 dan 1064 mg/m3 selama kebuntingan hari ke-6 sampai 11. Tahap berikutnya adalah pengamatan dan pemeriksaan ekspresi VEGF dan MAPK dengan pewarnaan imunohistokimia terhadap plasenta tikus putih yang telah dipapar dengan carbon black. Hasil penelitian adalah terjadi peningkatan ekspresi VEGF pada plasenta tikus yang dipapar carbon black dosis 523 mg/m3 dan 1064 mg/m3 per inhalasi selama masa pertengahan (6-11) kebuntingan. Selain itu terjadi peningkatan ekspresi MAPK pada plasenta tikus yang dipapar carbon black dosis 523 mg/m3 dan 1064 mg/m3 per inhalasi selama masa pertengahan(6-11) kebuntingan. Simpulan penelitian ini adalah ekspresi VEGF dan MAPK meningkat seiring dengan peningkatan dosis carbon black dan umur kebuntingan.
PENGARUH pH LENDIR MUKOSA VAGINA SAAT BIRAHI TERHADAP PERSENTASE KEBUNTINGAN (Conception Rate) PADA SAPI PERAH DI KUD TANI WILIS KABUPATEN TULUNGAGUNG DAN KSU TUNAS SETIA BARU KABUPATEN PASURUAN Andaru Rizki; Pudji Srianto; Endang Suprihati; Trilas Sardjito; Ismudiono Ismudiono; Mohammad Anam Al Arif
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.312 KB) | DOI: 10.20473/ovz.v8i2.2019.154-158

Abstract

The purpose of this research is to determine the effect of mucos acidity of vaginal mucous when estrus towards conception rate at KUD Tani Wilis Tulungagung District and KSU Tunas Setia Baru Pasuruan District. Measured samples were taken from 30 dairy cattles in each KUD. Mucos acidity of vaginal mucous were obtained by using a digital acidity meter before insemination was done on dairy cattle. After measuring the acidity from mucosal vagina mucous, artificial insemination has done on estrus dairy cattle. On the 60th day after insemination, rectal palpation was done to check the conception rate. Research result from 30 dairy cattle on each KUD showed that mucos acidity of vaginal mucous has signifficant effect towards conception rate. Mucos acidity of vaginal mucous range 6.5-8.5 with the highest range of conception rate in KSU Tunas Setia Baru Pasuruan District 7.5-7.7 and in KUD Tani Wilis Tulungagung District with the highest range of conception range 7.2-7.4 and 7.5-7.7.
CONCEPTION RATE DAN SERVICE PER CONCEPTION PADA SAPI PERANAKAN LIMOUSIN DAN SAPI PERANAKAN ONGOLE HASIL IB DI KECAMATAN PILANGKENCENG KABUPATEN MADIUN Bhaskara, Alfiutha Propha; Suprayogi, Tri Wahyu; Darsono, Roesno; Srianto, Pudji; Sardjito, Trilas; Hariadi, Mas’ud
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.328 KB) | DOI: 10.20473/ovz.v7i2.2018.152-155

Abstract

The study was conducted to know conception rate and service per conception of Limousin Cross breed and Ongole Cross Breed in Pilangkenceng, Madiun. Data were collected for the periode of Oktober 2016 to February 2017 from inseminators in Pilangkenceng district. The data were taken by primary and secondary. Primary data retrieval were done by direct observation, which includes several variables, where the variables include: the identity of the breeder, feeding and drinking. The secondary data were obtained by recording the farmer’s artificial insemination card. All data was analyzed to find a percentage of conception rate (CR) and find number of service per conception (S/C). Then S/C were analyzed with T-test adn CR were analyzed with chi-square test. The results of this research showed that the service per conception’s average of limousin cross breed and ongole cross breed is 1,61 and 1,72. The average of conception rate limousin cross breed and ongole cross breed is 53% % and 59 %. The result showed no significant difference (p>0,05) on CR and S/C between limousin cross breed and ongole cross breed. The conclusion of this research showed that service per conception and conception rate of ongole cross breed in Pilengkenceng Madiun is better than limousin cross breed but there’s no significant difference (p>0,05).
PERBANDINGAN BODY CONDITION SCORE, SERVICES PER CONCEPTION, CALVING INTERVAL, DAN DAYS OPEN SAPI FRIESIAN HOLSTEIN (FH) DI KEMITRAAN GREENFIELD DENGAN KUD TANI WILIS SENDANG Aghid Cahya P.; Sri Mulyati; Suwarno Suwarno; Imam Mustofa; Pudji Srianto; Budi Utomo
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.497 KB) | DOI: 10.20473/ovz.v8i2.2019.149-153

Abstract

The purpose of this study was to determine the comparison of Body Condition Score, Services Per Conception, Calving Interval, and Days Open cow friesian holstein (FH) in Greenfield Partnership and Tani Wilis Sendang Cooperation. The material used in this research is dairy cattle that have been inseminated in Greenfield Partnership and KUD Tani Wilis with secondary data retrieval of reproduction notes during IB until partus and quisioner distributed by farmers and inseminators. The exploratory survey method used is secondary data obtained from the record reproduction inseminator. The observed variables BCS, S/C, CI and DO . The data was processed using descriptive analysis to get a number BCS, S/C, CI and DO in Greenfield Partnership and Tani Wilis Sendang Cooperation. The results showed that the average value of BCS at Greenfield Partnership is 4.86 and at Tani Wilis Cooperation is 4.74. The average value of S/C in Greenfield Partnership is 4.36 and at Tani Wilis Sendang Cooperation is 2.06. The average value CI at Greenfield Partnership is 463.00 and at Tani Wilis Sendang is 414.40. The average value DO at Greenfield Partnership is 64.84 and at Tani Wilis Sendang is 88.00. It can be concluded that the comparison of BCS in both places is not significantly different while for the S/C of Tani WilisSendang Cooperation is better than Greenfield Partnership, the CalvingInterval in both places is still less good because it is far from the standard CI value, and for the Days Open Greenfield partnership is better thanTaniWilisSendang Cooperation.
Kualitas spermatozoa post-thawing pejantan sapi Friesian Holstein pada umur yang berbeda Widya Aulia Ristiani; Muhammad Yunus; Tri Wahyu Suprayogi; Pudji Srianto; Imam Mustofa; Rimayanti Rimayanti
Ovozoa : Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (647.153 KB) | DOI: 10.20473/ovz.v9i1.2020.12-16

Abstract

This study aimed to determine the post-thawing spermatozoa quality of Holstein-Friesian (HF) bulls at different ages. The samples were HF frozen semen of 3, 5, and 8-years -old age group produced by the Singosari National Artificial Insemination Center.  Nine samples of each group were randomly purposive sampling, thawed in 37ºC water for 15 seconds, and assessed on the percentage of viability, motility, and abnormality of spermatozoa. The obtained data were analyzed using the ANOVA method, followed by Duncan's multiple range test at a 95% confidence level. The result showed that post-thawing spermatozoa viability in 5 and 8-year-old HF bull were 67 ± 6.67%, and 78 ± 7.59, both were higher (p <0.05) compared to 62.78 ± 5.76% in HF bulls 3-yearsold group. Likewise, the motility of those bull aged 5 and 8 years (69.44 ± 6.82 and 65.00 ± 9.68%, respectively) were higher (p <0.05) compared to 55, 56 ± 5.83% in 3-year-old HF bull. Meanwhile, the percentage of post-thawing spermatozoa abnormalities in the three age groups were 4.33 ± 1.96%, 5.89 ± 1.90%, and 5.33 ± 1.41%, not significantly different (p> 0.05) respectively. It concluded that the best quality of post-thawing HF semen based on the percentage of viability, motility, and morphologic abnormality was collected from 5-8 years old bull. Nevertheless, spermatozoa from the three age goups still meet the Indonesia standard quality of frozen semen for artificial insemination.
PERSENTASE KEJADIAN KAWIN BERULANG SAPI PERAH YANG TELAH DI IB (INSEMINASI BUATAN) TINGKAT PETERNAK DESA NYAWANG KIDUL DI KUD TANI WILIS KECAMATAN SENDANG KABUPATEN TULUNGAGUNG Arda Wahyu Setyadi; Pudji Srianto; Oky Setyo Widodo; Wurlina Wurlina; Abdul Samik; Tjuk Imam Restiadi
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.664 KB) | DOI: 10.20473/ovz.v8i2.2019.164-168

Abstract

Dairy cows farm business level of KUD Tani Wilis in Nyawang Kidul, Sendang district, Tulungagung city has some problems resulting low livestock productivity. One of the causes of low levels of dairy reproductivity is the incidence of repeat breeder. Repeat breeder is a condition of a female cow that failed to breed after being mated three or more times with a normal and healthy fertilized bull. The main purpose of this research is to know the risk factor of repeat breeder in dairy cows at farmer level of KUD Tani Wilis in nyawang kidul, Sendang district, Tulungagung city. The type of research is descriptive research. This research was using 32 dairy cows from 6 breeders, the selection of cattles used Simple Random Sampling method. The collection data using questionnaires and direct observations in farms resulted that the evidence rate of dairy cattle's repeat breeder in this research is 45.7%. The results also showed that the influence of knowledge of farmers on disease or reproductive disorders positively correlated with the evidence of repeat breader.
KADAR MILK UREA NITROGEN (MUN) PADA SAPI PERANAKAN FRIESIAN HOLSTEIN (PFH) BERDASARKAN CALVING INTERVAL YANG BERBEDA Hanif Sabekti Pratama; Imam Mustofa; Widya Paramita Lokapirnasari; Pudji Srianto; Rimayanti Rimayanti; Suzanita Utama
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.387 KB) | DOI: 10.20473/ovz.v8i1.2019.1-4

Abstract

Milk urea nitrogen (MUN) analys may be used as a signal potential problem in feeding program. The aim of this research was to know levels of milk urea nitrogen with different calving interval. This research was using purposive sampling in KUD Tani Wilis, Sendang, Tulungagung. The sample was obtained from breeder as much as 16 samples, grouped into 2 groups, group 1 calving interval ≤370 and group 2 calving interval ≥450. This samples tested in Surabaya laboratory of health (Balai Besar Laboratorium Kesehatan Surabaya). The result showed that MUN not signifcanly different with different calving interval (p>0,05). The conclusion of this research was MUN did not related with different calving interval.
UJI BERBAGAI ALAT DETEKSI BIRAHI TERHADAP ANGKA KEBUNTINGAN PADA SAPI PERAH DI KOPERASI TUNAS SETIA BARU KABUPATEN PASURUAN Silvia Rani Andriyanti; Mas’ud Hariadi; Roesno Darsono; Pudji Srianto; wurlina wurlina; Tjuk Imam Restiadi
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.453 KB) | DOI: 10.20473/ovz.v7i2.2018.156-159

Abstract

This research aims to determine the use of various estrus detection devices before artificial insemination as measured by pregnancy rate. The researcher used a sample of 60 cows were used in this research which is divided into four groups as a control and treatment groups (Thermopin®, Hauptner and Draminski groups), and each cow was detected for the sign of estrus by three devices that were Thermopin®, Hauptner and Draminski prion when doing an artificial insemination. The cows were observed to 45 days after artificial insemination for observing pregnancy. This research method is the exploration scope to prove the effect of various estrus detection tool against pregnancy rates. The result of ANOVA test, there is the effect of using a various estrus detection devices against pregnancy rates. Samples tested by Draminski with average (1.53±0.51) resulted in a 53,3% pregnancy rate, the samples were tested with Hauptner with average (1.67±0,48) resulted in pregnancy rate 66,7%. Without treatment or control, sample with an average of (1.67±0,48) resulted in a  73,3% pregnancy rate. While samples were tested with Thermopin® with average (2.00±0,00) resulted in a 100% pregnancy rate. In conclusion, Thermopin® the best detection tool than other estrus detection devices.
CONCEPTION RATE DAN SERVICE PER CONCEPTION PADA SAPI BALI HASIL INSEMINASI BUATAN DI KABUPATEN SUMBAWA BARAT TAHUN 2017 Asih Deskayanti; Trilas Sardjito; Agus Sunarso; Pudji Srianto; Tri Wahyu Suprayogi; Herry Agoes Hermadi
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.879 KB) | DOI: 10.20473/ovz.v8i2.2019.159-163

Abstract

The study was conducted to know Conception Rate and Service per Conception of  Bali cattle acceptors in west Sumbaw district. Data were collected fot the periode of January to September 2017 from inseminator in west Sumbawa district. The data were taken by primary and secondary data. Primary data retrieval is done by direct observation, which includes several variables, where the variable include the identitiy of the breeder, the cages, feeding, drinking and knowladge of breeders. The secondary data were obtained by recording of the officer insemination. All data was analyzed to find a percentage of Conception Rate and find number of Service per Conception (S/C) by analyzed descriptive of SPSS. Risults of this research showed that Conception Rate and Service per Conception’s average of Bali Cattle is 60 % and 1,7. The conculusion of this research showed that Conception Rate is lower than normal value and Service per Conception of Bali cattle in West Sumbawa district is better. This research was expected to be a reference for the next artificial insemination programmed by the government.